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High purity solvents for HPLC become increasingly important as mobile phase caused by increasing sensitivity of HPLC systems. Therefore the analytics-shop.com offers solutions with different levels of purity from J.T. Baker and Merck:

  HPLC grade Gradient grade LC-MS grade
Solutions  2-Propanol, Acetonitril
Methanol, Water 
Aceton, Dichlormethan,
Isooctan, Tetrahydrofuran 
2-Propanol, Acetonitril,
Methanol, Water 
Properties  -suitable for isocratic separations
- designed for UV or fluorescence
   detection

- low fluorescence, slight waste steam
   pressure

- low acidity and alkalinity 
- high UV permeability for UV-Vis
   and fluorescence measurements
- slight influence on the baselinie of UV
   detection
- suitable for highly sensitive measure-
   ments
- decreasing of trace minerals, organics and ions
- very low ambient noise
- suitable for for measurements with high sensitivity  

For more information about the levels of purity please click here.

Attention: The takeover of Sigma-Aldrich by the company Merck was accompanied by provisions of the cartel office. To meet those provisions, the sale of Fluka solvents (formerly part of Sigma) was now passed on to Honeywell. In the Analytics-Shop you will continue to receive any solvents of the brand Fluka.

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Solvents for HPLC

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  1. Water, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L Water, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L
    Sale
    J.T. Baker

    Water, Baker HPLC Analyzed, HPLC Gradient Grade

    Regular Price: €140.48

    Special Price €69.00

    SKU: JB4218.2500
    In Stock
  2. Methanol, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L Methanol, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L
    Sale
    J.T. Baker

    Methanol, Baker HPLC Analyzed, HPLC Gradient Grade

    Regular Price: €231.00

    Special Price €75.00

    SKU: JB8402.2500
    In Stock
  3. Acetonitril, Baker HPLC Analyzed, Ultra Gradient HPLC Grade 4 x 2,5L Acetonitril, Baker HPLC Analyzed, Ultra Gradient HPLC Grade 4 x 2,5L
    Sale
    J.T. Baker

    Acetonitril, Baker HPLC Analyzed, Ultra Gradient

    Regular Price: €864.36

    Special Price €149.00

    SKU: JB9017.2500
    In Stock
  4. 2-Propanol (Iso-Propanol) by Baker HPLC, 4 x 2,5L glass bottle 2-Propanol (Iso-Propanol) by Baker HPLC, 4 x 2,5L glass bottle
    Sale
    J.T. Baker

    2-Propanol (Iso-Propanol) JT Baker HPLC

    Regular Price: €427.52

    Special Price €192.00

    SKU: JB9095.2500
    In Stock
  5. Merck Millipore

    Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €68.50
    SKU: MC1000142500
    In Stock
  6. Acetonitrile hypergrade for LC-MS LiChrosolv, , 4 x 2,5 L Acetonitrile hypergrade for LC-MS LiChrosolv, , 4 x 2,5 L
    Sale
    Merck Millipore

    Acetonitrile hypergrade for LC-MS LiChrosolv, , 4 x 2,5 L

    Regular Price: €892.00

    Special Price €215.00

    SKU: MC1000292500
    In Stock
  7. Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L
    Sale
    Merck Millipore

    Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L

    Regular Price: €868.00

    Special Price €157.00

    SKU: MC1000302500
    In Stock
  8. Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L
    Sale
    Merck Millipore

    Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L

    Regular Price: €270.00

    Special Price €75.00

    SKU: MC1060072500
    In Stock
  9. Methanol hypergrade for LC-MS LiChrosolv, 2,5 L Methanol hypergrade for LC-MS LiChrosolv, 2,5 L
    Sale
    Merck Millipore

    Methanol hypergrade for LC-MS LiChrosolv, 2,5 L

    Regular Price: €278.00

    Special Price €132.00

    SKU: MC1060352500
    In Stock
  10. Merck Millipore

    Ethanol gradient grade for liquid chromatography LiChrosolv

    €123.55
    SKU: MC1117272500
    In Stock
  11. Merck Millipore

    Water for chromatography LiChrosolv, 1 L

    €16.90
    SKU: MC1153331000
    In Stock
  12. Water for chromatography LiChrosolv, 2,5 L Water for chromatography LiChrosolv, 2,5 L
    Sale
    Merck Millipore

    Water for chromatography LiChrosolv, 2,5 L

    Regular Price: €140.00

    Special Price €69.00

    SKU: MC1153332500
    In Stock
  13. Acetonitril, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L Acetonitril, Baker HPLC Analyzed, HPLC Gradient Grade 4 x 2,5L
    Sale
    J.T. Baker

    Acetonitril, Baker HPLC Analyzed, HPLC Gradient Grade

    Regular Price: €633.36

    Special Price €114.00

    SKU: JB9012.2500GL
    In Stock
  14. Acetonitril LC-MS, 4 x 2,5L glass bottle Acetonitril LC-MS, 4 x 2,5L glass bottle
    Sale
    J.T. Baker

    Acetonitril LC-MS, 2,5L glass bottle

    Regular Price: €1,073.48

    Special Price €209.00

    SKU: JB9821.2500GL
    In Stock
  15. J.T. Baker

    Water for HPLC, LC-MS Grade, 6 x 1L glass bottle

    Regular Price: €204.60

    Special Price €168.00

    SKU: JB9825.1000GL
    In Stock
  16. Methanol, Baker HPLC Analyzed, LC-MS Grade 4 x 2,5L Methanol, Baker HPLC Analyzed, LC-MS Grade 4 x 2,5L
    Sale
    J.T. Baker

    Methanol, Baker HPLC Analyzed, LC-MS Grade

    Regular Price: €316.28

    Special Price €149.00

    SKU: JB9822.2500GL
    In Stock
  17. METHANOL, LC-MS CHROMASOLV(R), >=99.9%, 4 x 2,5 L METHANOL, LC-MS CHROMASOLV(R), >=99.9%, 4 x 2,5 L
    Sale
    Riedel-de Haen

    METHANOL, LC-MS CHROMASOLV(R), >=99.9%

    Regular Price: €206.96

    Special Price €112.00

    SKU: RD34966-4X2.5L
    In Stock
  18. ACETONITRILE LC-MS CHROMASOLV., 4 x 2,5 L ACETONITRILE LC-MS CHROMASOLV., 4 x 2,5 L
    Sale
    Riedel-de Haen

    ACETONITRILE LC-MS CHROMASOLV.

    Regular Price: €969.20

    Special Price €172.00

    SKU: RD34967-4X2.5L
    In Stock
  19. Riedel-de Haen

    ACETONE, 99.9+%, HPLC GRADE

    €42.71
    SKU: RD270725-1L
    In Stock
  20. ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, 4 x 2,5 L ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, 4 x 2,5 L
    Sale
    Riedel-de Haen

    ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE

    Regular Price: €882.02

    Special Price €111.50

    SKU: RD34851-4X2.5L
    In Stock
  21. METHANOL G CHROMASOLV FUER DIE GRADIENTE, 4 x 2,5 L METHANOL G CHROMASOLV FUER DIE GRADIENTE, 4 x 2,5 L
    Sale
    Riedel-de Haen

    METHANOL G CHROMASOLV FUER DIE GRADIENTE

    Regular Price: €209.71

    Special Price €41.94

    SKU: RD34885-4X2.5L
    In Stock
  22. ACETONITRILE G CHROMASOLV, FOR HPLC,SUPER GRADIENT GRADE, 4 x 2,5 L ACETONITRILE G CHROMASOLV, FOR HPLC,SUPER GRADIENT GRADE, 4 x 2,5 L
    Sale
    Riedel-de Haen

    ACETONITRILE G CHROMASOLV, FOR HPLC,SUPER GRADIENT GRADE

    Regular Price: €1,130.80

    Special Price €770.00

    SKU: RD34998-4X2.5L
    In Stock
  23. Merck Millipore

    Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €62.00
    SKU: MC1000142511
  24. Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L
    Sale
    AppliChem

    Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L

    Regular Price: €205.80

    Special Price €174.93

    SKU: AP361736.1612
  25. Riedel-de Haen

    WATER, HPLC GRADE, 1 L

    €22.98
    SKU: RD270733-1L
  26. AppliChem

    Acetic acid 100 % HPLC grade,2,5 L

    €86.37
    SKU: AP361008.1612
  27. AppliChem

    Chloroform HPLC grade,2,5 L

    €74.30
    SKU: AP361252.1612
  28. AppliChem

    iso-Hexane HPLC grade,2,5 L

    €132.60
    SKU: AP365261.1612
  29. Fluka

    FORMIC ACID 50%, 500 ml

    €130.56
    SKU: FL09676-500ML
  30. Fluka

    ORTHO-PHOSPHORIC ACID 85%, FOR HPLC

    €57.26
    SKU: FL79606-500ML
  31. Merck Millipore

    2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €76.50
    SKU: MC1096342500
  32. Merck Millipore

    2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €250.00
    SKU: MC1096346010
  33. Fluka

    SODIUM ACETATE ANHYDROUS, FOR HPLC, 50 g

    €46.70
    SKU: SA71185-50G
  34. Riedel-de Haen

    HEXANE, CHROMASOLV, FOR HPLC, >=95%

    €143.91
    SKU: RD270504-2.5L
  35. Riedel-de Haen

    N,N-DIMETHYLACETAMIDE, 99.9+%, HPLC GRADE

    €101.09
    SKU: RD270555-1L
  36. Riedel-de Haen

    ACETONE, CHROMASOLV PLUS, FOR HPLC, 99.

    €46.99
    SKU: RD650501-1L
  37. Riedel-de Haen

    2-PROPANOL, CHROMASOLV PLUS, FOR HPLC,

    €135.77
    SKU: RD650447-2.5L
  38. Fluka

    POTASSIUM HYDROXIDE SOLUTION 45%, FOR HPLC

    €23.95
    SKU: FL03564-100ML
  39. Fluka

    Triethylammine, for HPLC, 10 x 2 ml

    €112.05
    SKU: FL17924-10X2ML
  40. Fluka

    ORTHO-PHOSPHORIC ACID 85%, FOR HPLC

    €23.64
    SKU: FL79606-100ML
  41. Fluka

    ORTHO-PHOSPHORIC ACID 50%, FOR HPLC

    €92.62
    SKU: FL79607-500ML
  42. Fluka

    SULFURIC ACID 50%, FOR HPLC, 100 ml

    €51.91
    SKU: FL84733-100ML
  43. J.T. Baker

    2-Propanol (Iso-Propanol) by Baker HPLC, 6 x 1L glass bottle

    Regular Price: €322.80

    Special Price €259.82

    SKU: JB9095.1000
  44. Merck Millipore

    Ethanol for spectroscopy Uvasol

    €186.55
    SKU: MC1009802500
  45. Merck Millipore

    Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €54.02
    SKU: MC1009831000
  46. Merck Millipore

    Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €50.52
    SKU: MC1009831011
  47. Merck Millipore

    Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur

    €205.10
    SKU: MC1009835000
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Information about the different levels of purity for HPLC 

 For modern HPLC high purity solutions as mobile phases are increasingly needed. Our consumers can mainly choose between three different classes of purity for their individual analysis, HPLC grade, Gradient grade and LC-MS grade.

But whats the difference between the different qualities referring chromatography and between other high purity solvents? And why is it important to choose a solvent with reference to the performed application?

All qualities have the same basic material and a nominal high purity with low traces of xenobiotics (99.8 - 99.9 %, depending on the product) in common. But especially these minimal residue can cause problems in HPLC depending in the technic of detection, so that in this case "pure" is not equal to "pure".

The following describes the differences of the three levels of solvent purity and the reasons for using every quality for your application. 

HPLC grade

HPLC grade describes a solutions quality, that is mainly desinged for isocratic separations applying UV and fluorescence detection. These solvents are especially characerized by high purity combined with high UV permeability at all relevant wave lengths, low inherent fluorescence and waste steam pressure as well as low acidity and alkalinity.

The solvents undergo special treatment stages to remove potentially interferring substances. Thereby disturbing peaks are minimized and a low ambient noise can be enabled.

After the treatment these solutions are tested specificly for aptitude for fluorescence detection in HPLC. Through this quality controll continous purity of solvents as well as reproducability for your measurements can be guaranteed.

Gradient grade

For chromatographical separation in HPLC besides isocratic also gradient elutions are often applied. At these composition of mobile phase doesn´t change wuring measurement., so e.g. of reversed phase chromatography the organic part increases. Using solvents, that are not specially designed for gradient separation, can cause a distinct increasing of the base line at UV detection and can be sensitive for sensitivity of the method.

To ensure optimal chromatography taking gradient into account Gradient grade is further cleaned up. Besides suitability for UV and fluorescence detection all solutions are specially tested for applicability for gradiental separation, so that a charge consistent quality can be guaranteed for Gradient grade solutions.

The following figure shows a gradient profile to demonstrade the extraordinary results that can be attained with acetonitril Gradient grade. Only a low drift of the base line can be recorded.

Fig. 1: Drift of the base line using Gradient grade as solvent. 

LC-MS grade

With the success of (ultra) high pressure liquid chromatography mass spectroscopy (UHPLC-MS) many new requirements arised to the solvent used because of high detection sensitivity.

This way the focus by using mass spectroscopy lies especially on high ionisation efficience with concurrent low ion background. Therefore the solvents are applicable cleaned up. Thereby removing trace elements, organics and (metall) ions has first priority. These can cause an increased ambient noise and adduct formation with the substances, that should be analysed. The result is a decreased detection sensability.

For quality controll all solvents are tested versus Reserpin (m/z 609.49) as standard. The following figure shows the scan of a positiv and negativ ESI and APCi modus. The whole tested mass area displays a low noise ambient as well as no signals cause by impurities.

Fig. 2: Noise ambient of LC-MS measurements with LC-MS grade solvents.

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