HPLC Column Configurator

Find and compare HPLC Columns with our HPLC Column Configurator

With the help of our Liquid Chromatography Column Configurator, you can easily find the HPLC column you are looking for. Choose from over 140,000 HPLC columns from more than 40 well-knowned manufacturers and brands, such as Agilent Technologies, Grace, Merck Millipore, Thermo Scientific, Waters etc. In addition, we offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative.

There are many different manufacturers of HPLC columns. If you are looking for a particular HPLC column for your laboratory analysis, we can provide you with an independent offer of comparable columns from different manufacturers. If you are not satisfied with the performance of a column, our sales team will be able to offer you alternatives to suit your requirements.

By using the drop-down menus on the left, you can easily select the column description, packing material, particle size and other specifications as well as the desired manufacturer. If you are unable to find a suitable HPLC column or have any questions, please feel free to contact us.

For use in regulated environments, our HPLC columns are labeled with the universal USP code. Further information on official methods and HPLC column comparisons can be found on the United States Pharmacopeia (USP) website. Furthermore, we have a list of USP column suggestions which can be downloaded here.

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Product Image of Column Storage Cabinet, 30 columns (up to 300mm length)
Column Storage Cabinet, 30 columns (up to 300mm length)
Item number: AAColStore30
Brand: Altmann Analytik

In stock

€401.72 €446.36
Product Image of HPLC Column Chromolith Performance RP-18 endcapped 100x4.6mm, monolithic ready-to-use HPLC Column
HPLC Column Chromolith Performance RP-18 endcapped 100x4.6mm, monolithic ready-to-use HPLC Column
Item number: MC1021290001
Brand: Merck

In stock

€1,077.24
Product Image of UHPLC Column YMC-TriArt C18, 1,9µm, 75 x 3.0 mm
UHPLC Column YMC-TriArt C18, 1,9µm, 75 x 3.0 mm
Item number: YMTA12SP9-L503PT
Brand: YMC
€873.00
Product Image of HPLC Column PROTEIN KW-803, 300 Å, 5 µm, 8 x 300 mm
HPLC Column PROTEIN KW-803, 300 Å, 5 µm, 8 x 300 mm
Item number: SDF6989103
Brand: Shodex

In stock

€1,512.08
Product Image of HPLC Column Asahipak ODP-50 4E, 250 Å, 5 µm, 4.6 x 250 mm
HPLC Column Asahipak ODP-50 4E, 250 Å, 5 µm, 4.6 x 250 mm
Item number: SDF7620003
Brand: Shodex

In stock

€721.46 €901.83
Product Image of ProPac WCX-10 Analytical Column(4 x 250 mm)
ProPac WCX-10 Analytical Column(4 x 250 mm)
Item number: TF054993
Brand: Thermo Fisher Scientific

In stock

€1,152.95 €1,281.06
Product Image of HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm
HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm
Item number: WT186003034
Brand: Waters

In stock

€825.30 €917.00
Product Image of HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm
HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm
Item number: WTWAT046980
Brand: Waters

In stock

€789.00
Product Image of HPLC Column Symmetry C8 100Å 5µm 4.6x250mm
HPLC Column Symmetry C8 100Å 5µm 4.6x250mm
Item number: WTWAT054270
Brand: Waters

In stock

€881.00
Product Image of HPLC Column YMC-Pack ODS-AQ, 12 nm, S-5µm, 250 x 4.6 mm
HPLC Column YMC-Pack ODS-AQ, 12 nm, S-5µm, 250 x 4.6 mm
Item number: YMAQ12S05-2546WT
Brand: YMC

In stock

€754.00
Product Image of HPLC Column ZORBAX RR, SB-C18, 80Å, 3,5 µm, 4,6 x 100 mm
HPLC Column ZORBAX RR, SB-C18, 80Å, 3,5 µm, 4,6 x 100 mm
Item number: AG861953-902
Brand: Agilent Technologies
€612.00 €680.00
Product Image of HPLC Column CHIRALPAK® AD-H, 250 x 4,6 mm, 5 µm
HPLC Column CHIRALPAK® AD-H, 250 x 4,6 mm, 5 µm
Item number: DAIC19325
Brand: Chiral

In stock

€1,738.50 €1,830.00
Product Image of HPLC Column EC 125/4.6 NUCLEOSIL 100-5 C18
HPLC Column EC 125/4.6 NUCLEOSIL 100-5 C18
Item number: MN720002.46
Brand: Macherey-Nagel
€317.70 €353.00
Product Image of Reprosil Universal Reversed Phase (RP) Guard Column Cartridge 10x4mm for Columns with 3, 4 and 4.6mm ID, 5/PAK
Reprosil Universal Reversed Phase (RP) Guard Column Cartridge 10x4mm for Columns with 3, 4 and 4.6mm ID, 5/PAK
Item number: AAVRP-501040-5
Brand: Altmann Analytik
€233.33
Product Image of HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 125 mm, 12% Carbon
HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 125 mm, 12% Carbon
Item number: AANLI6RS-5012540
Brand: Altmann Analytik

In stock

€576.00 €640.00
Product Image of HPLC Column HYPERSIL BDS C18 5µm 250x4,6mm
HPLC Column HYPERSIL BDS C18 5µm 250x4,6mm
Item number: TF28105-254630
Brand: Thermo Fisher Scientific

In stock

€592.17 €696.67
Product Image of HPLC Column Vydac 214TP C4 250x4.6mm 5µm, old no: 5103926
HPLC Column Vydac 214TP C4 250x4.6mm 5µm, old no: 5103926
Item number: GR214TP54
Brand: Avantor

In stock

€1,020.00
Product Image of HPLC Column Nucleosil 100 C18, 5.0 µm, 4 x 125 mm, 15% Carbon, endcapped
HPLC Column Nucleosil 100 C18, 5.0 µm, 4 x 125 mm, 15% Carbon, endcapped
Item number: AANNS1C9-5012540
Brand: Altmann Analytik

In stock

€417.60 €464.00
Product Image of HPLC Column Osaka Soda (Shiseido) CAPCELL PAK C18 SG 120Å 5µm, 250mm x 4.6mm
HPLC Column Osaka Soda (Shiseido) CAPCELL PAK C18 SG 120Å 5µm, 250mm x 4.6mm
Item number: SHI-12513
Brand: Osaka Soda (former Shiseido)
€692.36 €769.29
Product Image of HPLC Column Discovery® C18, 5 µm, L x ID, 150 x 4.6 mm
HPLC Column Discovery® C18, 5 µm, L x ID, 150 x 4.6 mm
Item number: SU504955
Brand: Supelco
€932.53 €981.61
Product Image of HPLC Column Atlantis dc18 3µm 4.6 x 150mm
HPLC Column Atlantis dc18 3µm 4.6 x 150mm
Item number: WT186001342
Brand: Waters

In stock

€881.00
Product Image of HPLC Column YMC-Pack Pro C18, 12nm, S-3µm, 150 x 4.6 mm
HPLC Column YMC-Pack Pro C18, 12nm, S-3µm, 150 x 4.6 mm
Item number: YMAS12S03-1546WT
Brand: YMC
€834.00
Product Image of HPLC Column Inertsil ODS-3 100Å, 3µm, 4.6 x 150mm
HPLC Column Inertsil ODS-3 100Å, 3µm, 4.6 x 150mm
Item number: GL5020-01771
Brand: GL Sciences

In stock

€551.00 €580.00
Product Image of HPLC Column CHIRALPAK AGP, 100 x 4 mm, 5 µm
HPLC Column CHIRALPAK AGP, 100 x 4 mm, 5 µm
Item number: DAIC30713
Brand: Chiral

In stock

€1,235.00 €1,300.00
Product Image of HPLC Column EC 125/4 NUCLEOSIL 100-5 C18
HPLC Column EC 125/4 NUCLEOSIL 100-5 C18
Item number: MN720002.40
Brand: Macherey-Nagel
€317.70 €334.00
Product Image of HPLC Column EC 250/4 NUCLEOSIL 100-5 C8
HPLC Column EC 250/4 NUCLEOSIL 100-5 C8
Item number: MN720013.40
Brand: Macherey-Nagel
€407.00
Product Image of HPLC Column, EC 250/4.6 NUCLEOSIL 100-10 CN
HPLC Column, EC 250/4.6 NUCLEOSIL 100-10 CN
Item number: MN720024.46
Brand: Macherey-Nagel
€430.00
Product Image of HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
Item number: SU53829-U
Brand: Supelco

In stock

€911.06 €959.01
Product Image of HPLC Column Vydac 218TP C18 250x4.6mm 5µm, old no: 5103994
HPLC Column Vydac 218TP C18 250x4.6mm 5µm, old no: 5103994
Item number: GR218TP54
Brand: Avantor

In stock

€999.00
Product Image of HPLC Column Alltima C18 250x4.6mm 5µm old no: 88056
HPLC Column Alltima C18 250x4.6mm 5µm old no: 88056
Item number: GR5129678
Brand: Avantor

In stock

€819.90 €911.00
Product Image of HPLC Column Inertsil ODS-3 100Å, 5µm, 4.6 x 250mm
HPLC Column Inertsil ODS-3 100Å, 5µm, 4.6 x 250mm
Item number: GL5020-01732
Brand: GL Sciences

In stock

€560.50 €590.00
Product Image of HPLC Column CHIRALCEL® OD, 250 x 4,6 mm, 10 µm
HPLC Column CHIRALCEL® OD, 250 x 4,6 mm, 10 µm
Item number: DAIC14025
Brand: Chiral

In stock

€1,738.50 €1,830.00
Product Image of HPLC Column CHIRALPAK AD, 250 x 4.6 mm, 10 µm
HPLC Column CHIRALPAK AD, 250 x 4.6 mm, 10 µm
Item number: DAIC19025
Brand: Chiral

In stock

€1,738.50 €1,830.00
Product Image of HPLC Column PRP-X100, 100Å, 10 µm, 4.1 x 150 mm
HPLC Column PRP-X100, 100Å, 10 µm, 4.1 x 150 mm
Item number: HT79434
Brand: Hamilton
€867.83 €913.50
Product Image of HPLC Column SeQuant ZIC-HILIC, PEEK metallfrei, 3,5µm, 200Å, 150x2.1mm
HPLC Column SeQuant ZIC-HILIC, PEEK metallfrei, 3,5µm, 200Å, 150x2.1mm
Item number: MC1504480001
Brand: Merck
€1,250.00
Product Image of HPLC Column Hibar HR 50x2.1mm,Purospher STAR RP-18 endcapped, 3µm
HPLC Column Hibar HR 50x2.1mm,Purospher STAR RP-18 endcapped, 3µm
Item number: MC1506510001
Brand: Merck
€470.40 €588.00
Product Image of HPLC Cartridge LiChroCART 250x4mm, LiChrospher® 60 RP-select B, 5µm
HPLC Cartridge LiChroCART 250x4mm, LiChrospher® 60 RP-select B, 5µm
Item number: MC1508390001
Brand: Merck
€717.77
Product Image of UPLC Column Acquity BEH Phenyl 150x2.1mm 1.7 µm 130 Å, 1 - 12pH Range, Low Silan. Act., 185 m2/g, EndCapped, Spher., 15% Carb. Load
UPLC Column Acquity BEH Phenyl 150x2.1mm 1.7 µm 130 Å, 1 - 12pH Range, Low Silan. Act., 185 m2/g, EndCapped, Spher., 15% Carb. Load
Item number: WT186003378
Brand: Waters

In stock

€1,036.00
Product Image of HPLC Column ZORBAX RR, Eclipse, XDB-C8, 80Å, 3,5 µm, 4,6 x 150 mm
HPLC Column ZORBAX RR, Eclipse, XDB-C8, 80Å, 3,5 µm, 4,6 x 150 mm
Item number: AG963967-906
Brand: Agilent Technologies
€795.00
Product Image of HPLC Column Poroshell 120 EC-C18, 2,7 µm, 4,6 x 150 mm
HPLC Column Poroshell 120 EC-C18, 2,7 µm, 4,6 x 150 mm
Item number: AG693975-902
Brand: Agilent Technologies

In stock

€748.80 €832.00
Product Image of HPLC Column Prevail Organic Acid 150x4.6mm 5µm, old no: 88640
HPLC Column Prevail Organic Acid 150x4.6mm 5µm, old no: 88640
Item number: GR5129777
Brand: Avantor
€1,230.00
Product Image of HPLC Column EC 250/4 NUCLEOSIL 100-5 C18 HD
HPLC Column EC 250/4 NUCLEOSIL 100-5 C18 HD
Item number: MN720280.40
Brand: Macherey-Nagel
€655.00
Product Image of HPLC Column EC 250/3 NUCLEOSIL 100-5 C18 AB 250x3mm
HPLC Column EC 250/3 NUCLEOSIL 100-5 C18 AB 250x3mm
Item number: MN720936.30
Brand: Macherey-Nagel
€555.00
Product Image of HPLC Column, EC 150/3 NUCLEOSHELL PFP, 2.7 µm
HPLC Column, EC 150/3 NUCLEOSHELL PFP, 2.7 µm
Item number: MN763536.30
Brand: Macherey-Nagel
€720.00
Product Image of HPLC Cartridge LiChroCART 250x4mm, ChiraDex, 5µm
HPLC Cartridge LiChroCART 250x4mm, ChiraDex, 5µm
Item number: MC1513330001
Brand: Merck

In stock

€1,490.00
Product Image of HPLC Column ZORBAX SB-Aq, 80Å, 3,5 µm, 3 x 100 mm, Hardware Kit: 82888-91 Recommended Guard Cart.
HPLC Column ZORBAX SB-Aq, 80Å, 3,5 µm, 3 x 100 mm, Hardware Kit: 82888-91 Recommended Guard Cart.
Item number: AG861954-314
Brand: Agilent Technologies

In stock

€680.00
Product Image of HPLC Column with customized packing LiChrospher® 60 Si, 5µm, Hibar RT 250x4mm, customized packing
HPLC Column with customized packing LiChrospher® 60 Si, 5µm, Hibar RT 250x4mm, customized packing
Item number: MC1004247109
Brand: Merck
€1,137.14
Product Image of HPLC Cartridge LiChroCART 250x4mm, Superspher® 60 RP-8
HPLC Cartridge LiChroCART 250x4mm, Superspher® 60 RP-8
Item number: MC1160100001
Brand: Merck
€734.40 €918.00
Product Image of HPLC Cartridge LiChroCART 125x4mm, Superspher® 100 RP-18 endcapped
HPLC Cartridge LiChroCART 125x4mm, Superspher® 100 RP-18 endcapped
Item number: MC1168550001
Brand: Merck
€490.20 €817.00
Product Image of HPLC Cartridge LiChroCART 250x4mm, Superspher® 60 RP-8 endcapped
HPLC Cartridge LiChroCART 250x4mm, Superspher® 60 RP-8 endcapped
Item number: MC1168570001
Brand: Merck
€734.40 €918.00

Find the right column from over 140,000 HPLC columns from more than 50 different manufacturers such as Agilent, Macherey-Nagel or Merck. We also offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative. Compare prices and the application possibilities of the various manufacturers. If you are unable to find a suitable HPLC column, please feel free to contact us.

Prior to the development of the Reversed Phase (RP), Normal Phase Chromatography was the most common separation mode. We offer Normal Phase (NP) HPLC columns by all well-known manufacturers here.

In Normal Phase Chromatography, the mobile phase is non-polar and the stationary phase is polar (common: silica gel). This technique relies on the interaction of analytes with polar functional groups on the surface of the stationary phase. This interaction is strongest when using non-polar solvents as the mobile phase. The least polar compounds elute first and the most polar compounds elute last.

Normal Phase Chromatography is a very effective separation method because a wide range of solvents can be used to fine-tune the selectivity of a separation. However, it has become less popular with many chromatographers because of the complexity involved. Under certain conditions, long equilibration times or reproducibility problems may occur. This is due mainly to the sensitivity of the technique to the presence of low concentrations of polar contaminants in the mobile phase. Controlling these problems effectively produces better chromatograms than reverse phase methods as the commonly used solvents have a lower viscosity.

HPLC columns for Reversed Phase (RP) Chromatography

In Reversed Phase Chromatography, the polarity of the phases are "reversed". The mobile phase is polar and the stationary phase is non-polar. As it is one of the most popular separation modes in chromatography, all related products of all well-known manufacturers are available here.

The non-polar side chains in Reversed Phase Chromatography columns are bound either to a polymer or to a structure made of silica gel, which leads to them being hydrophobic. The longer the chain, the more non-polar the phases are. Polar analytes are eluted from the column first, followed by the non-polar analytes. Reversed phase is more widely practised than normal phased as they can be used universally for polar and non-polar analytes. In addition, this method is very sensitive and flexible as small changes in the composition of the mobile phase (e.g. salts, pH, organic solvents) or the temperature can completely change the separation properties of the system. RP-HPLC is used especially in numerous applications of UV spectroscopy (LC-UV).

Chiral columns for Chiral/Enantiomeric chromatography

Unlike normal HPLC columns, chiral or enantiomeric HPLC can also be used to separate and determine chiral compounds. This requires special chiral stationary phases that have fixed chiral functionalities. At Analytics-Shop, we have over 3,300 different chiral columns from different manufacturers, including the high-quality columns from Chiral Technologies as well as lower-priced alternatives of the same quality from YMC and our own Altmann Analytik brand.

Special columns such as chiral or enantiomeric HPLC columns are required to separate chiral compounds. With suitable interaction, these enable the separation of the enantiomers which have almost identical physical and chemical properties. Enantiomers are molecules with a chiral center. Diastereomers are molecules with two or more chiral centers, differ in their chemical properties and can be separated using chromatography. The separation of enantiomers is based on the formation of diastereomers and their interaction with other chiral molecules like chiral stationary phases (e.g. silica gels) in chromatographic separations. A conventional solvent is used as the eluent.

UHPLC and UPLC® columns

With Ultra High Performance Liquid Chromatography (UHPLC), dramatic increases in resolution, speed and sensitivity of HPLC can be achieved by using short and thin columns. The particles of the filling material have diameters of less than 2 µm, which results in an improved separation performance compared to standard HPLC. The increased total surface area of the filling material gives the analyte a higher adsorption ability. Analysis times are reduced due to the shorter columns and less solvent is required.

Columns for Hydrophilic Interaction Chromatography (HILIC)

Hydrophilic interaction chromatography (HILIC) is a popular alternative to normal phase and reverse phase chromatography. Similar to NP, buffer systems are used as stationary phases in HILIC mode. These are aqueous buffer systems with organic solvents, e.g. B. acetonitrile. In HILIC mode, water is the strongest eluant. HILIC columns are suitable for the separation of polar compounds such as carbohydrates, polar metabolites and hydrophilic compounds regardless of charge and molecular size. An extensive range of HILIC columns from established quality manufacturer YMC is available in our shop.

Columns for Supercritical Fluid Chromatography (SFC)

Significant advantages of Supercritical Liquid Chromatography (SFC) columns compared to HPLC columns:

  • Inexpensive: reduced solvent comsumption
  • Environmentally friendly
  • Time-saving: quick separation with high resolution
  • Flexible: chiral and achiral phases

Supercritical fluids show low viscosities and higher diffusivities when used as a mobile phase, resulting in narrower peaks due to rapid diffusion and faster elution and less pressure drop through the column. Supercritical fluids combine the benefits of liquids and gases, hence enabling the SFC technique to combine the best aspects of HPLC and gas chromatography (GC). In most cases, a supercritical fluid such as carbon dioxide is used as the mobile phase. The low viscosity of supercritical carbon dioxide enables analytical separations that are 3-5 times faster than those for normal phase HPLC. The speed of SFC separations, the preservation of organic solvents and more concentrated product fractions make SFC a preferred chromatographic technique for separating and purifying chemical mixtures.

SFC is an environmentally friendly separation technique that makes use of CO2-based mobile phases. The use of high-performance preparative columns (internal diameter of 10 - 50 mm) with a large number of particle sizes from 3 - 20 μm leads to the quick separation and recovery of cleaned components.

Nano HPLC columns

Nano HPLC uses columns with very small inner diameters. Columns with internal diameters of 75 µm, 100 µm or 150 µm are commonly used. Due to the reduction in the internal diameter, injection and flow rates must be reduced which is particularly advantageous when only small or diluted sample quantities are available. The smaller nano HPLC columns lead to an increased sensitivity with lower solvent consumption. Nano columns are able to maintain a high concentration of the injected sample and to direct approx. 40-50 % of the sample to the detector. Nano HPLC has a high separation efficiency compared to the traditional HPLC technique.

Columns for Gel Permeation Chromatography (GPC) & Size Exclusion Chromatography (SEC)

Gel permeation chromatography (GPC) is particularly suitable for the separation of non-polar molecules and can be used for a wide range of solvents, from non-polar organics to aqueous applications. GPC/SEC columns are packed with very small porous beads. In GPC/SEC, the molecules are separated according to size (size exclusion chromatography, SEC). The smaller molecules can enter the pores more easily and therefore spend more time in these pores, eluting last. Conversely, larger molecules spend little if any time in the pores and are eluted quickly. The GPC columns are filled with a microporous packing material and gel, hence the name gel permeation.

Ion Exchange Chromatography (IEC)

Ion exchange chromatography (IEC) separates molecules based on the respective charged groups on the surface of a protein. Molecules undergo electrostatic interactions wirh opposite charges on the stationary phase matrix.

The pH value of the buffer, the buffer concentration, packing of the columns and the salt gradient, in which the salt ions compete with the desired proteins to bind to charged groups on the surface of the medium, are important aspects affecting the results of IEC. At any pH, a protein has a net positive or negative charge due to the state of charge of the amino acids. If this protein is positively charged, it will bind to negatively charged materials and vice versa. The separation is based essentially on the charge and size of the ions.

Resins such as polystyrene, cellulose and crosslinked polyacrylamide or polydextran gels are used as mediums for ion exchange. The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged and anion-exchange chromatography is when the stationary phase is positively charged. Ion exchange chromatography is usually performed at pH values in which the protein has a net charge opposite to that of the stationary phase matrix.

Columns with polar bound phases

Polar-bound phases are based on silica gels which have chains attached onto the silica structure with functional groups. Chromatography makes use of the difference in polarity to separate a mixture. Separation takes place through different mechanisms and often as a result of the combination of several processes like adsorption, distribution, ion exchange, molecular size exclusion etc.

Permitted changes according to USP (United States Pharmacopeia)

The U.S. Pharmacopeia Convention is a scientific non-profit company that sets the standards for ingredients, concentration, quality and purity of medicines, food ingredients and food supplements that are manufactured, distributed and consumed all over the world. According to USP regulations, the following deviations may occur:

USP Chapter

  1. Column Length± 70%
  2. Column Inner Diameter: can be changed if the flow rate is kept constant.
  3. Particle Size: - 50%
  4. Flow Rate± 50%
  5. Ratio of components in the mobile phase± 30% or ± 10%
  6. pH value of the mobile phase± 0,2
  7. Salt concentration in the buffer± 10%
  8. Column Temperature± 10°C
  9. Wave Length of the UV-Vis detector± 3 nm
  10. Injection Volume: can be reduced until precision and detection limits are reached.

Permitted changes according to EP (European Pharmacopeia)

European Pharmacopeia Ph. Eur., Chapter 2.2.46

The European Pharmacopeia is a published collection of monographs describing the individual and general quality standards of ingredients, dosages and analytical methods of medicine. The aim is to set common quality standards across Europe to control the quality of medicines and other chemical products. According to the EP regulation, the following deviations can occur:

Isocratic elution

  1. Column Length: ± 70%
  2. Column Inner Diameter: ± 25%
  3. Particle Size: - 50%
  4. Flow Rate: ± 50%
  5. Ratio to components in the mobile phase: ± 30% or ± 2% absolute
  6. pH value of the mobile phase: ±0,2
  7. Salt concentration in the buffer: ± 10%
  8. Column Temperature: ± 10°C
  9. Wave Lenght of the detector: ± 3 nm
  10. Injection Volume: can be reduced until precision and detection limits are reached.

Gradient elution

  1. Column Length: ± 70%
  2. Column Inner Diameter: ± 25%
  3. Particle Size: no changes permitted
  4. Flow Rate: changes acceptable if column size is changed
  5. Ratio to components in the mobile phase: small changes in the composition of the mobile phase and the (density) grade are acceptable if the system configuration still meets the requirements.
  6. Dwell Volume: gradient instants can be used to compensate for differences in dwell volume between different systems.
  7. pH value of the mobile phase: no changes permitted
  8. Salt concentration in the buffer: no changes permitted
  9. Column Temperature: ± 5°C
  10. Wave Lenght of the detector: no deviations permitted
  11. Injection Volume: can be reduced until precision and detection limits are reached.
Phase Name USP Number Column Recommendations
Octadecylsilane chemically bound to porous silica gel L1 Potential Columns
Porous silica gel L3 Potential Columns
Octylsilane was chemically bound to completely porous silica gel L7 Potential Columns
A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel L8 Potential Columns
Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger L9 Potential Columns
Nitrile groups chemically bound to porous silica gel L10 Potential Columns
Phenyl groups chemically bound to porous silica gel L11 Potential Columns
Trimethyl groups chemically bound to porous silica gel L13 Potential Columns
Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger L14 Potential Columns
Methylsilane groups chemically bound to completely porous silica gel L15 Potential Columns
Dimethylsilane chemically bound to porous silica gel L16 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form L17 Potential Columns
Amino and cyano groups chemically bound to porous silica gel, 3 to 10 μm particle size L18 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form L19 Potential Columns
Dihydroxypropane groups chemically bound to porous silica gel L20 Potential Columns
Stable, spherical styrene-divinylbenzene copolymer L21 Potential Columns
A cation exchange resin of porous polystyrene having sulfonic acid groups L22 Potential Columns
Ion exchange resin of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups L23 Potential Columns
Pack with the ability to separate compounds (in a molecular weight range of 100 to 5000 daltons (determined with polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Polymethacrylic resin cross-linked with polyhydroxilated ether (surface containing residual carboxyl group content) was found to be appropriate L25 Potential Columns
Methylsilane is chemically bound to completely porous silica gel L26 Potential Columns
Chiral ligand exchange material with L-proline copper complex covalently bound to broken silica gel L32 Potential Columns
Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form L34 Potential Columns
Polymethacrylate gel pack with the ability to separate proteins in a molecular weight range between 2,000 and 40,000 daltons by molecular size. L37 Potential Columns
Size exclusion Pack for water soluble paints based on methacrylate L38 Potential Columns
Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin L39 Potential Columns
Cellulose tris-3,5-dimethylphenylcarbamate on porous silica gel L40 Potential Columns
Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel L41 Potential Columns
Pentafluorophenyl groups are chemically bound to silica gel L43 Potential Columns
High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups L47 Potential Columns
Amylose tris-3,5-dimethylphenylcarbamate on porous, spherical silica gel L51 Potential Columns
Ovomucoid (chiral recognition protein). Chemically bound to silica particles L57 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the sodium (Na +) form L58 Potential Columns
Spherical, porous silica gel with a covalent surface modification with alkylamide groups with end capping L60 Potential Columns
C30 silane is bound to a completely porous silica gel L62 Potential Columns