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HPLC columns for ion exchange chromatography

In ion exchange chromatography, the net charge and the charge distribution on the surface of a protein determine the interaction of the protein with the charged groups on the surface of the packing material. The surface charges of protein and packing material must be opposite, so that an interaction can take place. The modification of different parameters, such as the pH value of the buffer, the buffer composition, the gradient of the gradient, and the salt with which the gradient is built, enable a wide range of possibilities for optimizing a successful separation. At each pH, a protein has a positive or negative net charge due to the charge state of the amino acids. Accordingly, this protein, if positively charged, will bind either to negatively charged materials or otherwise to positively charged materials. The separation takes place essentially according to charge and size of the ions. As materials for the gel matrix, resins such as polystyrene, cellulose, and crosslinked polyacrylamide or polydextran gels are used. It is distinguished by strong and weak anion or cation exchangers. Ion exchange chromatography is normally performed at pH values which leave the protein in its active conformation.

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Ion-Exchanger (IEC)

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  1. Thermo Fisher Scientific

    ProPac SCX-10 Analytical Column (4 x 250 mm)

    €984.02
    SKU: TF054995
  2. Waters

    HPLC Column Protein-Pak Hi-Res CM 4.6 x 100mm

    €1,741.00
    SKU: WT186004929
  3. Altmann Analytik

    HPLC Column Partisil SAX 10µm 250x4

    €708.00
    SKU: AANPSSX-10025040
  4. Agilent Technologies

    HPLC Column Agilent Bio SA x , 4.6 x 250 mm, 5µm, SS

    €1,455.00
    SKU: AG5190-2465
  5. Hamilton

    PRP-X100 10µm 4.1x150mm

    €768.79
    SKU: HT79434
  6. Hamilton

    PRP-X200 10µm 4.1x150mm

    €740.52
    SKU: HT79441
  7. Hamilton

    PRP-X100 10µ 4.6x250mm PEEK

    €859.65
    SKU: HT79455
  8. Thermo Fisher Scientific

    ProPac SAX-10 Analytical Column (4 x 250 mm)

    €984.02
    SKU: TF054997
  9. Thermo Fisher Scientific

    ProPac SAX-10 Analytical Column (2 x 250 mm)

    €984.02
    SKU: TF063448
  10. Thermo Fisher Scientific

    ProPac SCX-10 Analytical Column (2 x 250 mm)

    €984.02
    SKU: TF063456
  11. Thermo Fisher Scientific

    ProSwift SAX-1S Monolith Column (4.6 x 50 mm)

    €872.86
    SKU: TF064293
  12. Thermo Fisher Scientific

    ProSwift SAX-1S (1 x 50 mm)

    €872.86
    SKU: TF068459
  13. Thermo Fisher Scientific

    100x4.6mm 3µ Hypersil GOLD SAX

    €636.16
    SKU: TF26303-104630
  14. Thermo Fisher Scientific

    150x2.1mm 3µ Hypersil GOLD SAX

    €702.20
    SKU: TF26303-152130
  15. Thermo Fisher Scientific

    150x3mm 3µ Hypersil GOLD SAX

    €702.20
    SKU: TF26303-153030
  16. Thermo Fisher Scientific

    50x2.1mm 5µ Hypersil GOLD SAX

    €555.64
    SKU: TF26305-052130
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