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Reversed Phase (RP) Chromatography Columns

Reversed phase LC columns by top HPLC columns manufacturers are available at Analytics Shop. Now, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar (hydrophobic) stationary phase. Most of these protocols use as the mobile phase an aqueous blend of water with a miscible, polar organic solvent, such as acetonitrile or methanol. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica (sometimes called ODS) is the most popular type of reversed-phase HPLC packing. Reversed-phase HPLC is very widely applicable for small molecule analyses. Reversed- phase HPLC is a good choice for peptide and protein separations when short chain alkyl stationary phases are used.

Reversed phase HPLC is the first choice for:

  • Neutral and non-polar compounds with a molecular weight less than 2000 Da
  • Homologs and Benzologs
  • Weak acids and bases
  • Strong acids and bases (ion pair HPLC)
  • Proteins and peptide

Narrow down your HPLC column search dimentions & quickly find an equivalent column in our HPLC Column Configurator.

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Reversed Phase (RP)

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  1. Agilent Technologies

    HPLC Column ZORBAX SB-C18, 80Å, 1,8 µm, 3 x 50 mm, 600 bar, SPL

    €592.00
    SKU: AG884950-576
  2. Waters

    HPLC Column Symmetry C8 100Å 5µm 4.6x250mm

    €710.00
    SKU: WTWAT054270
    In Stock
  3. Waters

    HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm

    €638.00
    SKU: WTWAT046980
    In Stock
  4. Waters

    HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm

    Regular Price: €754.00

    Special Price €678.60

    SKU: WT186003034
    In Stock
  5. Agilent Technologies

    HPLC Column ZORBAX RR, SB-C18, 80Å, 3,5 µm, 4,6 x 100 mm

    Regular Price: €544.00

    Special Price €489.60

    SKU: AG861953-902
    In Stock
  6. Waters

    HPLC Column Atlantis dc18 3µm 4.6 x 150mm

    €710.00
    SKU: WT186001342
  7. Thermo Fisher Scientific

    HPLC Column BDS-HYPERSIL-C18250x4.6mm 5µm

    €548.47
    SKU: TF28105-254630
    In Stock
  8. ACE

    HPLC Column ACE EXCEL 3 C18-PFP 150x2.1mm

    €622.00
    SKU: ACEXL-1110-1502U
    In Stock
  9. ACE

    HPLC Column ACE 5 C18 100x4.6mm

    €553.00
    SKU: ACACE-121-1046
    In Stock
  10. Agilent Technologies

    HPLC Column ZORBAX Rx-C18, 80Å, 5 µm, 4,6 x 250 mm

    €620.00
    SKU: AG880967-902
    In Stock
  11. Agilent Technologies

    HPLC Column ZORBAX Rx-C8, 80Å, 5 µm, 4,6 x 250 mm

    €620.00
    SKU: AG880967-901
    In Stock
  12. Agilent Technologies

    HPLC Column ZORBAX RR, Rx-C8, 80Å, 3,5 µm, 4,6 x 150 mm

    €651.00
    SKU: AG863953-906
    In Stock
  13. Altmann Analytik

    HPLC Column Nucleosil 100Å C18 5µm 250x4.6mm

    Regular Price: €419.75

    Special Price €362.25

    SKU: AANNS1C9-5025046
    In Stock
  14. Altmann Analytik

    HPLC Column Nucleosil 100Å C18 5µm 125x4mm

    Regular Price: €299.00

    Special Price €263.93

    SKU: AANNS1C9-5012540
    In Stock
  15. Altmann Analytik

    HPLC Column Hypersil 120Å ODS 5µm 250x4.6mm

    €517.00
    SKU: AANHSO-5025046
    In Stock
  16. Altmann Analytik

    HPLC Column Hypersil BDS C18 5µm 33x4,6 mm, endcapped, 120A

    €247.50
    SKU: AANHSBC9-503346
    In Stock
  17. Altmann Analytik

    HPLC Column Spherisorb 80Å ODS-2 5µm 125x4.6mm, endc.

    €356.50
    SKU: AANSSO2-5012546
    In Stock
  18. Altmann Analytik

    HPLC Column Lichrospher 60Å RP-Select-B 5µm 250x4mm

    Regular Price: €442.00

    Special Price €386.10

    SKU: AANLI6RS-5025040
    In Stock
  19. Altmann Analytik

    HPLC Column Lichrospher 60Å RP-Select-B 5µm 125x4.6mm

    Regular Price: €370.50

    Special Price €321.75

    SKU: AANLI6RS-5012546
    In Stock
  20. Altmann Analytik

    HPLC Column Lichrospher 60Å RP-Select-B 5µm 125x4mm

    Regular Price: €338.00

    Special Price €298.35

    SKU: AANLI6RS-5012540
    In Stock
  21. Altmann Analytik

    HPLC Column Spherisorb 80Å ODS-2 5µm 30x4mm

    €195.50
    SKU: AANSSO2-503040
  22. Altmann Analytik

    HPLC Column Reprosil Pur 120Å C8 3µm 100x4.6mm

    €354.00
    SKU: AANRSPU98-3010046
    In Stock
  23. Altmann Analytik

    HPLC Column Reprosil Pur 120Å ODS-3 3µm 150x4.6mm

    €420.00
    SKU: AANRSPU93-3015046
    In Stock
  24. Altmann Analytik

    HPLC Column Reprosil 100Å C8 4µm 125x4mm

    €354.00
    SKU: AANRS9C8-4012540
    In Stock
  25. Altmann Analytik

    HPLC Column Partisil ODS-3 10µm 20x4,6 mm, endcapped, 90A, Carbon 10%C

    €360.00
    SKU: AANPSO3-1002046
    In Stock
  26. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 250x3 mm, endcapped

    €546.25
    SKU: AANNS1C9A-5025030
    In Stock
  27. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 125x3 mm, endcapped

    €448.50
    SKU: AANNS1C9A-5012530
    In Stock
  28. Altmann Analytik

    HPLC Column Nucleosil 100 C18 5µm 200x4,6 mm, endcapped

    €396.75
    SKU: AANNS1C9-5020046
  29. Altmann Analytik

    HPLC Column Nucleosil 100 C18 10µm 20x4,6 mm, endcapped, 100A

    €166.75
    SKU: AANNS1C9-1002046
    In Stock
  30. Altmann Analytik

    HPLC Column Equisil Gold C18 3µm 150x3 mm, endcapped, 120A

    €456.00
    SKU: AANEQGC9-3015030
    In Stock
  31. Grace

    HPLC Column Prevail C18 50x4.6mm 3µm old no: 43829

    €604.00
    SKU: GR5126993
    In Stock
  32. Grace

    HPLC Column Alltima Phenyl 250x4.6mm 5µm old no: 88092

    Regular Price: €770.00

    Special Price €693.00

    SKU: GR5129692
    In Stock
  33. Grace

    HPLC Column Alltima C18 150x4.6mm 5µm old no: 88052

    Regular Price: €726.00

    Special Price €653.40

    SKU: GR5129675
    In Stock
  34. Shodex

    HPLC column Asahipak ODP-50 4E, 4,6x250mm 5 µm

    Regular Price: €875.56

    Special Price €744.23

    SKU: SDF7620003
    In Stock
  35. Grace

    HPLC Column Alltima C18 250x4.6mm 5µm old no: 88056

    Regular Price: €759.00

    Special Price €683.10

    SKU: GR5129678
    In Stock
  36. Grace

    HPLC Column AlltimaHP C18 250x4.6mm 5µm old no: 87680 ---sale---

    Regular Price: €780.00

    Special Price €702.00

    SKU: GR5129556
  37. Grace

    HPLC Column VYDAC 201TP C18 250x4.6mm 5µm, old no: 201TP54

    Regular Price: €822.00

    Special Price €739.80

    SKU: GR5103821
    In Stock
  38. YMC

    UHPLC Column YMC-TriArt C18, 1,9µm, 75 x 3.0 mm

    €720.00
    SKU: YMTA12SP9-L503PT
    In Stock
  39. YMC

    HPLC Column YMC ProC18, analytical, 12 nm, 5µm, 250 x 4.6 mm

    €699.00
    SKU: YMAS12S05-2546WT
    In Stock
  40. YMC

    HPLC Column YMC ProC18, analytical, 12 nm, 5µm, 150 x 4.6 mm

    €588.00
    SKU: YMAS12S05-1546WT
    In Stock
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