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Reversed Phase (RP) Chromatography Columns

Reversed phase LC columns by top HPLC columns manufacturers are available at Analytics Shop. Now, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar (hydrophobic) stationary phase. Most of these protocols use as the mobile phase an aqueous blend of water with a miscible, polar organic solvent, such as acetonitrile or methanol. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica (sometimes called ODS) is the most popular type of reversed-phase HPLC packing. Reversed-phase HPLC is very widely applicable for small molecule analyses. Reversed- phase HPLC is a good choice for peptide and protein separations when short chain alkyl stationary phases are used.

Reversed phase HPLC is the first choice for:

  • Neutral and non-polar compounds with a molecular weight less than 2000 Da
  • Homologs and Benzologs
  • Weak acids and bases
  • Strong acids and bases (ion pair HPLC)
  • Proteins and peptide

Narrow down your HPLC column search dimentions & quickly find an equivalent column in our HPLC Column Configurator.

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Reversed Phase (RP)

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  1. Agilent Technologies

    HPLC Column ZORBAX SB-C18, 80Å, 1,8 µm, 3 x 50 mm, 600 bar, SPL

    €592.00
    SKU: AG884950-576
  2. Waters

    HPLC Column Symmetry C8 100Å 5µm 4.6x250mm

    €710.00
    SKU: WTWAT054270
    In Stock
  3. Waters

    HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm

    €638.00
    SKU: WTWAT046980
    In Stock
  4. Waters

    HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm

    Regular Price: €754.00

    Special Price €678.60

    SKU: WT186003034
    In Stock
  5. Agilent Technologies

    HPLC Column ZORBAX RR, SB-C18, 80Å, 3,5 µm, 4,6 x 100 mm

    Regular Price: €544.00

    Special Price €489.60

    SKU: AG861953-902
    In Stock
  6. Waters

    HPLC Column Atlantis dc18 3µm 4.6 x 150mm

    €710.00
    SKU: WT186001342
  7. Thermo Fisher Scientific

    HPLC Column BDS-HYPERSIL-C18250x4.6mm 5µm

    €548.47
    SKU: TF28105-254630
    In Stock
  8. Agilent Technologies

    HPLC Column ZORBAX Rx-C18, 80Å, 5 µm, 4,6 x 250 mm

    €620.00
    SKU: AG880967-902
    In Stock
  9. Agilent Technologies

    HPLC Column ZORBAX Rx-C8, 80Å, 5 µm, 4,6 x 250 mm

    €620.00
    SKU: AG880967-901
  10. Agilent Technologies

    HPLC Column ZORBAX RR, Rx-C8, 80Å, 3,5 µm, 4,6 x 150 mm

    €651.00
    SKU: AG863953-906
    In Stock
  11. Altmann Analytik

    HPLC Column Spherisorb ODS-2 5µm 150x4,6

    €379.50
    SKU: AANSSO2-5015046
    In Stock
  12. Altmann Analytik

    HPLC Column Nucleosil 100 C18 5µm 250x4,6

    Regular Price: €402.50

    Special Price €362.25

    SKU: AANNS1C9-5025046
    In Stock
  13. Altmann Analytik

    HPLC Column Nucleosil 100 C18 5µm 125x4

    Regular Price: €293.25

    Special Price €263.93

    SKU: AANNS1C9-5012540
  14. Altmann Analytik

    HPLC Column Hypersil ODS 5µm 250x4,6

    €517.00
    SKU: AANHSO-5025046
    In Stock
  15. Altmann Analytik

    HPLC Column Hypersil ODS 5µm 125x4,6

    Regular Price: €396.00

    Special Price €356.40

    SKU: AANHSO-5012546
    In Stock
  16. Altmann Analytik

    HPLC Column Hypersil BDS C18 5µm 100x4,6

    €374.00
    SKU: AANHSBC9-5010046
    In Stock
  17. Altmann Analytik

    HPLC Column Spherisorb ODS-2 5µm 125x4,6, endc., 80A

    €345.00
    SKU: AANSSO2-5012546
    In Stock
  18. Altmann Analytik

    HPLC Column Lichrospher 60 RP-Select-B 5µm 250x4,6

    Regular Price: €455.00

    Special Price €409.50

    SKU: AANLI6RS-5025046
    In Stock
  19. Altmann Analytik

    HPLC Column Reprosil Pur 120 ODS-3 3µm 150x4,6

    €396.00
    SKU: AANRSPU93-3015046
    In Stock
  20. Altmann Analytik

    HPLC Column Reprosil 100 C8 4µm 125x4

    €348.00
    SKU: AANRS9C8-4012540
    In Stock
  21. Altmann Analytik

    HPLC Column Reprosil 80 ODS-1 3µm 100x4

    €336.00
    SKU: AANRS8O1-3010040
    In Stock
  22. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 250x3

    €546.25
    SKU: AANNS1C9A-5025030
    In Stock
  23. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 125x3

    €448.50
    SKU: AANNS1C9A-5012530
    In Stock
  24. Altmann Analytik

    HPLC Column Nucleosil 100 C18 10µm 20x4,6

    €166.75
    SKU: AANNS1C9-1002046
    In Stock
  25. Grace

    HPLC Column Prevail C18 50x4.6mm 3µm old no: 43829

    €574.12
    SKU: GR5126993
    In Stock
  26. Grace

    HPLC Column Alltima Phenyl 250x4.6mm 5µm old no: 88092

    Regular Price: €731.76

    Special Price €658.58

    SKU: GR5129692
    In Stock
  27. Grace

    HPLC Column Alltima C18 150x4.6mm 5µm old no: 88052

    Regular Price: €690.59

    Special Price €621.53

    SKU: GR5129675
    In Stock
  28. Shodex

    HPLC column Asahipak ODP-50 4E, 4,6x250mm 5 µm

    Regular Price: €875.56

    Special Price €744.23

    SKU: SDF7620003
    In Stock
  29. Grace

    HPLC Column Alltima C18 250x4.6mm 5µm old no: 88056 ---sale---

    Regular Price: €722.35

    Special Price €650.12

    SKU: GR5129678
    In Stock
  30. Grace

    HPLC Column VYDAC 201TP C18 250x4.6mm 5µm, old no: 201TP54

    Regular Price: €781.18

    Special Price €703.06

    SKU: GR5103821
    In Stock
  31. YMC

    UHPLC Column YMC-TriArt C18, 1,9µm, 75 x 3.0 mm

    €720.00
    SKU: YMTA12SP9-L503PT
    In Stock
  32. YMC

    HPLC Column YMC-Triart C18, analytical, nm, 3µm, 100 x 4.6 mm

    €544.00
    SKU: YMTA12S03-1046WT
    In Stock
  33. YMC

    HPLC Column YMC ProC18, analytical, 12 nm, 5µm, 250 x 4.6 mm

    €699.00
    SKU: YMAS12S05-2546WT
    In Stock
  34. YMC

    HPLC Column YMC ProC18, analytical, 12 nm, 5µm, 150 x 4.6 mm

    €588.00
    SKU: YMAS12S05-1546WT
    In Stock
  35. YMC

    HPLC Column YMC-Pack ODS-A, analytical, nm, 5µm, 250 x 4.6 mm

    €555.00
    SKU: YMAA12S05-2546WT
    In Stock
  36. Waters

    HPLC Column SYMMETRY C18 150 mm 4.6 mm 3.5 µm 100 Å

    Regular Price: €658.00

    Special Price €592.20

    SKU: WTWAT200632
    In Stock
  37. Waters

    HPLC Column NOVA-PAK C18 150 mm 3.9 mm 4 µm 60 Å

    €540.00
    SKU: WTWAT086344
    In Stock
  38. Waters

    HPLC Column SYMMETRY 100Å C18 75 mm 4.6 mm 3.5 µm 100 Å

    €576.00
    SKU: WTWAT066224
    In Stock
  39. Waters

    HPLC Column Symmetry C8 3.5µm 4.6X100mm

    €607.00
    SKU: WTWAT066204
    In Stock
  40. Waters

    HPLC Column Symmetry C18 100Å 5µm 4.6x250mm

    Regular Price: €710.00

    Special Price €639.00

    SKU: WTWAT054275
    In Stock
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