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Reversed Phase (RP) Chromatography Columns

Reversed phase LC columns by top HPLC columns manufacturers are available at Analytics Shop. Now, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar (hydrophobic) stationary phase. Most of these protocols use as the mobile phase an aqueous blend of water with a miscible, polar organic solvent, such as acetonitrile or methanol. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica (sometimes called ODS) is the most popular type of reversed-phase HPLC packing. Reversed-phase HPLC is very widely applicable for small molecule analyses. Reversed- phase HPLC is a good choice for peptide and protein separations when short chain alkyl stationary phases are used.

Reversed phase HPLC is the first choice for:

  • Neutral and non-polar compounds with a molecular weight less than 2000 Da
  • Homologs and Benzologs
  • Weak acids and bases
  • Strong acids and bases (ion pair HPLC)
  • Proteins and peptide

Narrow down your HPLC column search dimentions & quickly find an equivalent column in our HPLC Column Configurator.

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Reversed Phase (RP)

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  1. Agilent Technologies

    HPLC Column Zorbax SB-C18 1.8 µm, 3.0x50mm, 80 Å

    €592.00
    SKU: AG884950-576
  2. Agilent Technologies

    HPLC Column Zorbax SB-C18 3.5 µm, 4.6x100mm, 80 Å

    Regular Price: €544.00

    Special Price €489.60

    SKU: AG861953-902
    In Stock
  3. Waters

    HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm

    Regular Price: €739.00

    Special Price €665.10

    SKU: WT186003034
    In Stock
  4. Waters

    HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm

    Regular Price: €618.00

    Special Price €556.20

    SKU: WTWAT046980
    In Stock
  5. Waters

    HPLC Column Symmetry C8 100Å 5µm 4.6x250mm

    €704.00
    SKU: WTWAT054270
    In Stock
  6. Grace

    Vydac 214TP C4 HPLC Column 250x4.6mm 5µ, old no. 5103926

    Regular Price: €760.00

    Special Price €684.00

    SKU: GR214TP54
    In Stock
  7. Thermo Fisher Scientific

    250x4.6mm 5µ Hypersil BDS C18

    €537.72
    SKU: TF28105-254630
    In Stock
  8. Waters

    HPLC Column Atlantis dc18 3µm 4.6 x 150mm

    €694.00
    SKU: WT186001342
  9. Grace

    Vydac 218TP C18 HPLC Column 250x4.6mm 5µ, old no. 5103994

    Regular Price: €1,067.14

    Special Price €960.30

    SKU: GR218TP54
  10. Waters

    HPLC Column XBridge Phenyl 150 mm 4.6 mm 3.5 µm 135 Å

    €739.00
    SKU: WT186003335
    In Stock
  11. Waters

    UPLC Column Acquity BEH Phenyl 150x2.1mm 1.7 µm 130 Å

    €851.00
    SKU: WT186003378
    In Stock
  12. Waters

    HPLC Column Atlantis T3 C18 100 mm 3 mm 3 µm 100 Å

    €642.00
    SKU: WT186003722
    In Stock
  13. Waters

    HPLC Column Atlantis T3 C18 150 mm 3 mm 3 µm 100 Å

    €680.00
    SKU: WT186003723
    In Stock
  14. Waters

    HPLC Column XBridge Phenyl 250 mm 4.6 mm 3.5 µm 135 Å

    €807.00
    SKU: WT186003965
    In Stock
  15. Waters

    HPLC Column ACCQ-TAG 3.9X150mm

    €711.00
    SKU: WTWAT052885
    In Stock
  16. Agilent Technologies

    HPLC Column Agilent HC-C18(2), 4.6 x 250 mm, 5µm, 170Å

    €382.00
    SKU: AG588905-902
    In Stock
  17. Agilent Technologies

    HPLC Column Zorbax Eclipse x DB-C18, 5µm, 4.6 x 150 mm cart

    €491.00
    SKU: AG7995118-595
    In Stock
  18. Agilent Technologies

    HPLC Column Zorbax SB-C18 1.8 µm, 4.6x100mm, 80 Å

    €685.00
    SKU: AG828975-902
    In Stock
  19. Agilent Technologies

    HPLC Column Zorbax Rx-C8, 3.5 µm, 4.6x150mm, 80 Å

    €651.00
    SKU: AG863953-906
    In Stock
  20. Agilent Technologies

    HPLC Column Zorbax SB-C8, 3.5 µm, 4.6x150mm, 300 Å

    €620.00
    SKU: AG863973-906
    In Stock
  21. Agilent Technologies

    HPLC Column Zorbax RX -C8 Analytical 4.6 x 250mm, 5µm

    €620.00
    SKU: AG880967-901
    In Stock
  22. Agilent Technologies

    HPLC Column Zorbax RX -C18 Analytical 4.6 x 250mm, 5µm

    €620.00
    SKU: AG880967-902
    In Stock
  23. Agilent Technologies

    HPLC Column Zorbax SB-C18 5.0 µm, 4.6x250mm, 80 Å

    Regular Price: €620.00

    Special Price €558.00

    SKU: AG880975-902
    In Stock
  24. Agilent Technologies

    HPLC Column Zorbax SB-Phenyl, 5.0 µm, 4.6x250mm, 80 Å

    €620.00
    SKU: AG880975-912
    In Stock
  25. Agilent Technologies

    HPLC Column Zorbax SB-C18 5.0 µm, 4.6x150mm, 80 Å

    €566.00
    SKU: AG883975-902
    In Stock
  26. Agilent Technologies

    HPLC Column Zorbax SB-C8, 5.0 µm, 4.6x150mm, 80 Å

    €566.00
    SKU: AG883975-906
    In Stock
  27. Agilent Technologies

    HPLC Column Zorbax RRHD Eclipse Plus C18,2,1 x 50 mm, 1,8u, 1.8 µm

    €662.00
    SKU: AG959757-902
    In Stock
  28. Agilent Technologies

    HPLC Column Zorbax Eclipse x DB-C18 4.6 x 150,3.5u Rapid Resol

    €636.00
    SKU: AG963967-902
    In Stock
  29. Agilent Technologies

    HPLC Column Zorbax Eclipse x DB-C18 4.6 x 250, 5u Analytical

    €618.00
    SKU: AG990967-902
    In Stock
  30. Agilent Technologies

    HPLC Column Zorbax Eclipse x DB-C18 4.6 x 150 5u Analytical

    €565.00
    SKU: AG993967-902
    In Stock
  31. Macherey-Nagel

    HPLC Column, HPLC Column, EC 125/4 NUCLEOSIL 100-5 C18

    €273.00
    SKU: MN720002.40
    In Stock
  32. Macherey-Nagel

    HPLC Column, HPLC Column, EC 125/4.6 NUCLEOSIL 100-5 C18

    €288.00
    SKU: MN720002.46
  33. Macherey-Nagel

    HPLC Column, EC 250/4 NUCLEOSIL 100-5 C8

    €333.00
    SKU: MN720013.40
    In Stock
  34. Macherey-Nagel

    HPLC Column, HPLC Column, EC 250/4 NUCLEOSIL 100-5 C18

    €333.00
    SKU: MN720014.40
    In Stock
  35. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEOSIL 100-5 C18

    €352.00
    SKU: MN720014.46
    In Stock
  36. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEOSIL 100-10 C18

    €352.00
    SKU: MN720023.46
    In Stock
  37. Macherey-Nagel

    HPLC Column, EC 125/4.6 NUCLEOSIL 120-3 C18

    €413.00
    SKU: MN720040.46
    In Stock
  38. Macherey-Nagel

    HPLC Column, EC 150/4.6 NUCLEOSIL 100-5 C18

    €288.00
    SKU: MN720120.46
    In Stock
  39. Macherey-Nagel

    HPLC Column, EC 150/4.6 NUCLEOSIL 100-3 C18

    €413.00
    SKU: MN720949.46
    In Stock
  40. Macherey-Nagel

    HPLC Column, HPLC Column, EC 250/4 NUCLEODUR 100-5 C18 HPLC Column, EC

    Regular Price: €333.00

    Special Price €283.05

    SKU: MN760002.40
    In Stock
  41. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEODUR 100-5 C18 HPLC Column, EC

    Regular Price: €352.00

    Special Price €299.20

    SKU: MN760002.46
    In Stock
  42. Osaka Soda (former Shiseido)

    HPLC Column Osaka Soda (Shiseido) CAPCELL PAK C18 SG 120Å 5µm, 250mm x 4.6mm

    €716.80
    SKU: SHI-12513
    In Stock
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