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Hamilton HPLC Columns

Hamilton is not only a reputed producer of syringes but also of HPLC columns of outstanding quality. We have the whole range of 193 different polymer-based HPLC columns for Reversed Phase, Anion Ex- change, Cation exchange and Ion exchange separations. Besides HPLC columns of Hamilton the analytics-shop.com also offers GC autosampler syringes and spare syringes. Here you can get an overview of the product range by Hamilton:

Phase: PRP-1, PRP-3, PRP-X100, PRP-X110, PRP-X500, C18, C8, etc.

Packing Material: X100, X110/110S, X500, X600, etc.

Particle Size: 3 µm, 5 µm, 7 µm, 10 µm, etc.

 

If you have any questions about products by Hamilton, please do not hesitate to contact us or use our very own HPLC columns configurator.

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Hamilton HPLC Columns

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  1. Hamilton

    HPLC Column PRP-X200, 100Å, 10 µm, 4.1 x 150 mm

    Regular Price: €706.94

    Special Price €671.60

    SKU: HT79441
  2. Hamilton

    HPLC Column PRP-1, 100Å, 5 µm, 4.1 x 150 mm

    Regular Price: €733.92

    Special Price €697.22

    SKU: HT79444
  3. Hamilton

    HPLC Column PRP-1, 100Å, 5 µm, 7 x 305 mm

    Regular Price: €1,244.39

    Special Price €1,182.17

    SKU: HT79795
  4. Hamilton

    HPLC Guard Cartridge HC-75 PRP-X400 H(+), 12-20 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €332.28

    Special Price €315.67

    SKU: HT79134
  5. Hamilton

    HPLC Guard Column Starter Kit, HxSil C8, 100Å, 10 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge

    Regular Price: €408.38

    Special Price €387.96

    SKU: HT79135
  6. Hamilton

    HPLC Guard Column Starter Kit, HxSil C18, 100Å, 10 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge

    Regular Price: €408.38

    Special Price €387.96

    SKU: HT79137
  7. Hamilton

    HPLC Guard Cartridge HxSil C18, 100Å, 10 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €387.19

    Special Price €367.83

    SKU: HT79138
  8. Hamilton

    HPLC Guard Column Starter Kit, PRP-X400, 7 µm, 2.1 x 20 mm, 1 Holder, 2 Cartridges

    Regular Price: €464.24

    Special Price €441.02

    SKU: HT79224
  9. Hamilton

    HPLC Guard Cartridge Silica, 10 µm, 2.1 x 20 mm, 5 pc/PAK

    Regular Price: €288.94

    Special Price €274.49

    SKU: HT79450
  10. Hamilton

    HPLC Guard Cartridge HxSil C8, 100Å, 10 µm, 2.1 x 20 mm, 5 pc/PAK

    Regular Price: €300.50

    Special Price €285.47

    SKU: HT79451
  11. Hamilton

    HPLC Guard Cartridge HxSil C18, 100Å, 10 µm, 2.1 x 20 mm, 5 pc/PAK

    Regular Price: €288.94

    Special Price €274.49

    SKU: HT79452
  12. Hamilton

    HPLC Guard Column Starter Kit, Silica, 10 µm, 2.1 x 20 mm, 1 Holder, 2 Cartridges

    Regular Price: €263.90

    Special Price €250.70

    SKU: HT79457
  13. Hamilton

    HPLC Guard Column Starter Kit, HxSil C8, 100Å, 10 µm, 4.6 x 20 mm, 1 Holder, 2 Cartridges

    Regular Price: €261.98

    Special Price €248.88

    SKU: HT79458
  14. Hamilton

    HPLC Guard Column Starter Kit, HxSil C18, 100Å, 10 µm, 4.6 x 20 mm, 1 Holder, 2 Cartridges

    Regular Price: €261.01

    Special Price €247.96

    SKU: HT79459
  15. Hamilton

    HPLC Guard Cartridge PRP-X500 C(2+), 12-20 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €332.28

    Special Price €315.67

    SKU: HT79865
  16. Hamilton

    HPLC Guard Column PRP-1, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79286
  17. Hamilton

    HPLC Guard Column PRP-X100, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79287
  18. Hamilton

    HPLC Guard Column PRP-X200, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79288
  19. Hamilton

    HPLC Guard Column PRP-X300, 12-20 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79289
  20. Hamilton

    HPLC Guard Column PRP-X400, 7 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79290
  21. Hamilton

    HPLC Guard Column PRP-C18, 12-20 µm, 2.1 x 33 mm

    Regular Price: €375.63

    Special Price €356.85

    SKU: HT79291
  22. Hamilton

    HPLC Guard Column RCX-10, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79292
  23. Hamilton

    HPLC Guard Column PRP-X110, 12-20 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79293
  24. Hamilton

    HPLC Guard Column PRP-X800, 7 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79294
  25. Hamilton

    HPLC Guard Column PRP-3, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79295
  26. Hamilton

    HPLC Guard Column PRP-h5, 10 µm, 2.1 x 33 mm

    Regular Price: €375.63

    Special Price €356.85

    SKU: HT79296
  27. Hamilton

    HPLC Guard Column HxSil C8, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79298
  28. Hamilton

    HPLC Guard Column HxSil C18, 10 µm, 2.1 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79299
  29. Hamilton

    HPLC Guard Column PRP-1, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79912
  30. Hamilton

    HPLC Guard Column PRP-X100, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79913
  31. Hamilton

    HPLC Guard Column PRP-X200, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79914
  32. Hamilton

    HPLC Guard Column PRP-X300, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79915
  33. Hamilton

    HPLC Guard Column PRP-X400, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79916
  34. Hamilton

    HPLC Guard Column PRP-X400 H+, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79917
  35. Hamilton

    HPLC Guard Column PRP-C18, 12-20 µm, 4.6 x 33 mm

    Regular Price: €375.63

    Special Price €356.85

    SKU: HT79918
  36. Hamilton

    HPLC Guard Column PRP-X500 C+, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79919
  37. Hamilton

    HPLC Guard Column PRP-3, 12-20 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79920
  38. Hamilton

    HPLC Guard Column PRP-h5, 12-20 µm, 4.6 x 33 mm

    Regular Price: €375.63

    Special Price €356.85

    SKU: HT79921
  39. Hamilton

    HPLC Guard Column HxSil C8, 10 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79922
  40. Hamilton

    HPLC Guard Column HxSil C18, 10 µm, 4.6 x 33 mm

    Regular Price: €364.07

    Special Price €345.86

    SKU: HT79923
  41. Hamilton

    HPLC Column HxSil C8, 100Å, 5µm, 2.1 x 50 mm

    Regular Price: €462.30

    Special Price €439.19

    SKU: HT79107
  42. Hamilton

    HPLC Column HxSil C8, 100Å, 5µm, 2.1 x 100 mm

    Regular Price: €544.17

    Special Price €516.96

    SKU: HT79109
  43. Hamilton

    HPLC Column HxSil C8, 100Å, 3µm, 2.1 x 30 mm

    Regular Price: €489.28

    Special Price €464.82

    SKU: HT79116
  44. Hamilton

    HPLC Column HxSil C8, 100Å, 3µm, 2.1 x 50 mm

    Regular Price: €516.24

    Special Price €490.43

    SKU: HT79117
  45. Hamilton

    HPLC Guard Column Starter Kit, PRP-1, 12-20 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge

    Regular Price: €423.78

    Special Price €402.59

    SKU: HT79121
  46. Hamilton

    HPLC Guard Cartridge PRP-1, 100Å, 12-20 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €310.13

    Special Price €294.62

    SKU: HT79122
  47. Hamilton

    HPLC Guard Column Starter Kit, PRP-3, 12-20 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge

    Regular Price: €464.24

    Special Price €441.02

    SKU: HT79123
  48. Hamilton

    HPLC Guard Cartridge PRP-3, 100Å, 12-20 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €299.53

    Special Price €284.56

    SKU: HT79124
  49. Hamilton

    HPLC Guard Column Starter Kit, PRP-X100, 12-20 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge

    Regular Price: €423.78

    Special Price €402.59

    SKU: HT79125
  50. Hamilton

    HPLC Guard Cartridge PRP-X100, 100Å, 12-20 µm, 4.6 x 20 mm, 2 pc/PAK

    Regular Price: €310.13

    Special Price €294.62

    SKU: HT79126
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PRP

Hamilton Polymers - Poly(styrene divinylbenzene) or PS-DVB is a robust polymer material used as the base framework for all the Hamilton polymer HPLC packing materials. The high degree of cross-linking with divinylbenzene makes the polymer bead extremely durable for high pressure applications. The most important properties are:

- Can handle pressures over 5000psi
- Have a usable pH range of 1‐13 (allows the separation of biological samples in their natural state)
- Polymer columns can be cleaned with 0.1 M sodium hydroxide to remove strongly retained material.
- Swelling in organic solvents such as THF or chloroform is negligible because of the cross‐linking
- Can be operated at much higher temperatures as compared to silica based materials
- Excellent sample recoveries due to the lack of acidic silanol groups
- PS‐DVB resins are similar in retention characteristics to a C18 but do have a slightly different selectivity in some cases.

Phase
 
Particle Size
in µm 
Phase
 
Particle Size
in µm 
PRP-1 3, 5, 7, 10 PRP-h1 5
PRP-3 3, 10 PRP-h5 5

 

Anion Exchange Columns

Six polymeric column packings for the separation of inorganic and organic anions, DNA and carbohydrates are offered from Hamilton. The most important properties of each column are:

- X100: Polymeric column packing for separation of inorganic nad organic anions from 10 ppb to 500 ppm.
- X110/110S: Polymeric anion exchange column packing for separation of inorganic and organic anions from 20 ppb to 20 ppm.
- X500: Polymeric strong-base anion exchange packing with limited porosity for separation of proteins.
- X600: Polymeric weak-base anion exchange packing for gradient separation of proteins and DNA oligomers.
- RCX-10: Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates.
- RCX-30: Polymeric anion exchange packing for isocratic or gradient separation of complex carbohydrates. 

Phase
 
Particle Size
in µm 
Phase
 
Particle Size
in µm 
PRP-X100 3, 10 PRP-X600 7
PRP-X110 7 RCX-10 7
PRP-X110S 7 RCX-30 7
PRP-X500 7    

 

Cation Exchange Columns

A few polymeric column packings for separation of inorganic and organic cations, glyphosate, organic acids, and sugars are offered from Hamilton. The most important properties are:
- X200: Analyze inorganic and organic cations using conductivity or UV detection. Separate mono or divalent cations depending on mobile phase conditions from 20 ppb to 200 ppm.
- X400: Analyze glyphosate and its metabolite in drinking water. The PRP-X400 provides unique hydrophilic interaction separations.
- X800: Separate transition metals or mono and divalent cations in the same run.
- HC75: Calcium, hydrogen and lead form columns for the separation of mono and disaccharides, organic acids, sugars, and sugar alcohols.

Phase
 
Particle Size
in µm 
Phase
 
Particle Size
in µm 
PRP-X200 10 PRP-X800 7
PRP-X400 7 HC-75 9

 

Ion Exclusion Columns

Hamilton also offers one HPLC column for ion exclusion chromatography, PRP-X300. This HPLC column is a polymeric, ion exclusion column packing for the separation of alcohols and organic acids 

Phase
 
Particle Size
in µm 
PRP-X300 7

 

HX-Sil

Hamilton HxSil C8 and C18 columns exhibit greater retention than most columns. This allows the separation of compounds that are not sufficiently retained on other C8 or C18 columns.
- C8: Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C18.

- C18: Hamilton HxSil C18 columns are well end-capped for retention time reproducibility and durability. HxSil C18 columns exhibit greater retention than most columns and enhances the separation of compounds that are not sufficiently retained on other C18 columns.

Phase
 
Particle Size
in µm 
Phase
 
Particle Size
in µm 
C18 3, 5 C8 5

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