Sigma-Aldrich HPLC Columns

Supelco HPLC Columns

Supelco HPLC columns are available in different configurations:

Column Description: Ascentis, Bioshell, Discovery, Supelcosil, Supelcoguard

Packing Material: CN, C8, C18, Amide, Phenyl Hexyl

Pore Size: 90Å, 100Å, 120Å, 300Å

If you have any questions regarding Supelco HPLC columns, please do not hesitate to contact our product experts. To compare HPLC columns of various manufacturers within seconds, you may use our very own HPLC Column Configurator.

 
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Product Image of HPLC Column Supelcosil™ Suplex™ pKb-100, 5 µm, 25 cm x 4.6 mm
HPLC Column Supelcosil™ Suplex™ pKb-100, 5 µm, 25 cm x 4.6 mm
Item number: SU58934
Brand: Supelco

In stock

€785.57
Product Image of HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
Item number: SU53829-U
Brand: Supelco

In stock

€931.00 €980.00
Product Image of HPLC Column Ascentis Express AQ-C18, 90 Å, 2.7 µm, 75 x 3 mm
HPLC Column Ascentis Express AQ-C18, 90 Å, 2.7 µm, 75 x 3 mm
Item number: SU577329-U
Brand: Supelco
€850.11
Product Image of HPLC-Column Discovery BIO Wide Pore C8, 300A, 10µm, 25 cm x 21.2 mm
HPLC-Column Discovery BIO Wide Pore C8, 300A, 10µm, 25 cm x 21.2 mm
Item number: SU567225-U
Brand: Supelco
€3,893.25
Product Image of HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x  4.6 mm, 3 pc/PAK
HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x 4.6 mm, 3 pc/PAK
Item number: SU63334-U
Brand: Supelco
€691.03
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 4.6 mm
Item number: SU63329-U
Brand: Supelco
€1,141.88
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 4.6 mm
Item number: SU63328-U
Brand: Supelco
€1,042.91
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x 4.6 mm
Item number: SU63327-U
Brand: Supelco
€1,071.34
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 4.6 mm
Item number: SU63325-U
Brand: Supelco
€833.96
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 4.6 mm
Item number: SU63324-U
Brand: Supelco
€713.40
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 4.6 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 4.6 mm
Item number: SU63322-U
Brand: Supelco
€713.40
Product Image of HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x  3.0 mm, 3 pc/PAK
HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x 3.0 mm, 3 pc/PAK
Item number: SU63315-U
Brand: Supelco
€691.03
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 3.0 mm
Item number: SU63314-U
Brand: Supelco
€1,141.88
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 3.0 mm
Item number: SU63313-U
Brand: Supelco
€1,042.91
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x  3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x 3.0 mm
Item number: SU63311-U
Brand: Supelco
€1,071.34
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 3.0 mm
Item number: SU63308-U
Brand: Supelco
€833.96
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 3.0 mm
Item number: SU63307-U
Brand: Supelco
€713.40
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 3.0 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 3.0 mm
Item number: SU63306-U
Brand: Supelco
€713.40
Product Image of HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x  2.1 mm, 3 pc/PAK
HPLC Guard Cartridge BIOshell IgG, 1000A, C4, 2.7 µm, 5 mm x 2.1 mm, 3 pc/PAK
Item number: SU63291-U
Brand: Supelco
€691.03
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 15 cm x 2.1 mm
Item number: SU63289-U
Brand: Supelco
€1,141.88
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 10 cm x 2.1 mm
Item number: SU63288-U
Brand: Supelco
€1,042.91
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x  2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 7.5 cm x 2.1 mm
Item number: SU63284-U
Brand: Supelco
€1,185.38
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 5 cm x 2.1 mm
Item number: SU63283-U
Brand: Supelco
€833.96
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 3 cm x 2.1 mm
Item number: SU63282-U
Brand: Supelco
€713.40
Product Image of HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 2.1 mm
HPLC Column BIOshell IgG, 1000A, C4, 2.7 µm, 2 cm x 2.1 mm
Item number: SU63281-U
Brand: Supelco
€713.40
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 400 mm
Item number: SUK5176-U
Brand: Supelco
€750.89
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 330 mm
Item number: SUK5175-U
Brand: Supelco
€730.97
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 50 mm x 250 mm
Item number: SUK5174-U
Brand: Supelco
€697.00
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 400 mm
Item number: SUK5173-U
Brand: Supelco
€468.57
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 330 mm
Item number: SUK5172-U
Brand: Supelco
€460.37
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 250 mm
Item number: SUK5171-U
Brand: Supelco
€439.29
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 150 mm
HPLC Column Omnifit EZ Glass, I.D. x L 35 mm x 150 mm
Item number: SUK5170-U
Brand: Supelco
€411.17
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 400 mm
Item number: SUK5169-U
Brand: Supelco
€316.29
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 330 mm
Item number: SUK5168-U
Brand: Supelco
€308.09
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 250 mm
Item number: SUK5167-U
Brand: Supelco
€256.54
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 150 mm
HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 150 mm
Item number: SUK5166-U
Brand: Supelco
€256.54
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 100 mm
HPLC Column Omnifit EZ Glass, I.D. x L 25 mm x 100 mm
Item number: SUK5165-U
Brand: Supelco
€244.83
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 400 mm
Item number: SUK5164-U
Brand: Supelco
€233.11
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 330 mm
Item number: SUK5163-U
Brand: Supelco
€226.09
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 250 mm
Item number: SUK5162-U
Brand: Supelco
€212.03
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 150 mm
HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 150 mm
Item number: SUK5161-U
Brand: Supelco
€192.11
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 100 mm
HPLC Column Omnifit EZ Glass, I.D. x L 15 mm x 100 mm
Item number: SUK5160-U
Brand: Supelco
€181.57
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 400 mm
Item number: SUK5159-U
Brand: Supelco
€190.94
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 330 mm
Item number: SUK5158-U
Brand: Supelco
€210.86
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 250 mm
Item number: SUK5157-U
Brand: Supelco
€207.34
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 150 mm
HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 150 mm
Item number: SUK5156-U
Brand: Supelco
€181.57
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 100 mm
HPLC Column Omnifit EZ Glass, I.D. x L 10 mm x 100 mm
Item number: SUK5155-U
Brand: Supelco
€166.34
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 400 mm
HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 400 mm
Item number: SUK5154-U
Brand: Supelco
€274.11
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 330 mm
HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 330 mm
Item number: SUK5153-U
Brand: Supelco
€255.37
Product Image of HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 250 mm
HPLC Column Omnifit EZ Glass, I.D. x L 6.6 mm x 250 mm
Item number: SUK5152-U
Brand: Supelco
€202.66

Whether conventional HPLC, UHPLC, or in the analysis of complex compounds, products of Sigma Aldrich are an
essential part of modern analysis. Through continuous development and innovation, including the Fused-Core Technology,
HPLC columns from Sigma-Aldrich are among the best in the world. In the following the best known and most common packing
materials are described in detail.

Supelco HPLC Columns: Column Material Ascentis:

Ascentis HPLC columns provide high purity, stability, and thus highly reproducible results. Modern binding processes
optimize the stability of the phase, while undesirable secondary interactions are minimized. Depending on the separation
problem you can choose from a wide range of possible phases to achieve the best separation result. Ascentis HPLC columns
are ideal for the use in the LC-MS as in all other modern applications and are scalable form analytic to preparative.

Phase Particel Size (μm) Pore Size (Å) pH-Range Carbon Load Endcapping
C18 3, 5, 10 100 2-8 25 Yes
C8 3, 5, 10 100 2-8 15 Yes
Phenyl 3, 5 100 2-8 19 Yes

Sigma-Aldrich HPLC Columns: Column Material Ascentis Express:

Based on Fused-Core ® technology, Ascentis Express HPLC columns offer significant advantages compared to similar columns with fully-porous particles. Ascentis Express provides the speed and effectiveness of <2 micron particles, but with half the pressure. Due the reduced pressure Ascentis Express columns can be used in conventional HPLC and LC-MS-, UPLC-, and ultra-high-pressure systems. The reduced pressure also allows the use of longer columns, enabling improved resolution. Ascentis Express columns combine all these significant properties with high load capacity and excellent durability.

Phase Particel Size (μm) Pore Size (Å) pH-Range Carbon Load Endcapping
C18 2.7, 5 90 2-9 25 Yes
C8 2.7, 5 90 2-9 15 Yes
RIP-Amide 2.7, 5 90 2-9 19 Yes

Supelco HPLC Columns: Column Material Discovery:

Supelco Discovery HPLC Columns provide excellent peak sharpening, combined with improved resolution and efficiency.
Available are a wide field of phases, scalable from preparative to the analytical. Discovery HS columns are specially
designed pharmaceutical analysis and purifications.

Phase Particel Size (μm) Pore Size (Å) pH-Range Carbon Load Endcapping
C18 5 180 2-8 12 Yes
C8 5 180 2-8 7,5 Yes
Cyano 5 180 2-8 4,5 Yes
HS C18 3, 5, 10 120 2-8 20 Yes

Supelco HPLC Columns: Column Material apHera:

Supelco HPLC columns apHera have been developed to combine the advantages of silica and polystyrene columns.
apHera HPLC columns offer the same separation efficiency, but additionally allow the benefits of using buffered and
alkaline solutions. Furthermore, they allow to work in a pH range of 2-12. apHera amino-column based on covalently
bound amino groups and are ideally optimized for use in the LC-MS. They also have been specially optimized for the
separation of mono-and oligosaccharides.

Phase Particel Size (μm) Pore Size (Å) pH-Range Carbon Load Endcapping
C18 5, 9, 13 300 2-12 17 -
C8 5 300 2-12 10 -
C4 5 300 2-12 6 -
NH2 5, 9, 13 300 2-13 0 -

Supelco HPLC Columns: Column Material Kromasil:

Kromasil silicon-based HPLC columns are scalable from analytical to industrial scale and offer at all sizes the same
excellent effectiveness. They are characterized by high chemical and mechanical stability, which results in long life
and high loading-capacity. Due to their excellent chemical stability, the reproducibility of the results obtained is
optimized, while minimizing unwanted effects.

Phase Particel Size (μm) Pore Size (Å) pH-Range Carbon Load Endcapping
C18 3.5, 5 100, 300 20 -
C8 3.5, 5, 7, 10 100, 300 12 -
C4 3.5, 5, 7, 10 100, 300 8 -
Silica 3.5, 5 60, 100 0 -


Supelco HPLC Columns: Column Material Supelcosil:

Supelcosil silica-based HPLC columns are available in over 40 different bonded phases, in a large range of particle sizes
and column configurations and from analytical to preparative scale. Depending on the separation problem, there is always
the right phase to receive the best possible speration result.
 
If there are any questions or problems with our products the appointment our competent team will help you personally and
without any obligation.