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With increasing sensitivity of HPLC systems, it becomes essential to use high-purity solvents (overview of the purity levels) as mobile phases in HPLC. We offer solvents with various purity levels from J.T. Baker and Merck:

  HPLC grade Gradient grade LC-MS grade
Solvents 2-Propanol, Acetonitrile
Methanol, Water 
Acetone, Dichlormethane,
Isooctane, Tetrahydrofuran 
2-Propanol, Acetonitrile,
Methanol, Water 
Properties  - suitable for isocratic separations
- designed for UV or fluorescence
  detection
- low fluorescence, slight waste steam
  pressure
- low acidity and alkalinity 
- high UV-permeability for UV-Vis
  and fluorescence measurements
- slight influence on the baselinie of UV
   detection
- suitable for highly sensitive measure-
   ments
- decreasing of trace minerals, organics and ions
- very low ambient noise
- suitable for for measurements with high sensitivity  

 

Please click here for more information on the purity levels of solvents.

Please note: The takeover of Sigma-Aldrich by the company Merck was accompanied by provisions of the cartel office. To meet those provisions, the sale of Fluka solvents (formerly part of Sigma) has now been passed on to Honeywell. You will still be able to purchase solvents from the brand Fluka in our shop.

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Solvents for HPLC

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  1. Merck

    Water for chromatography LiChrosolv, 2,5 L

    Regular Price: €38.50

    Special Price €18.98

    SKU: MC1153332500
    In Stock
  2. Riedel-de Haen

    ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, Glass Bottle, 4 x 2.5 L

    Regular Price: €981.18

    Special Price €126.47

    SKU: RD34851-4X2.5L
    In Stock
  3. Riedel-de Haen

    Methanol, LC-MS CHROMASOLV(R), ≥99.9%, Glass Bottle, 4 x 2.5 L

    Regular Price: €225.79

    Special Price €131.59

    SKU: RD34966-4X2.5L
    In Stock
  4. Riedel-de Haen

    ACETONITRILE LC-MS CHROMASOLV, Glass Bottle, 4 x 2.5 L

    Regular Price: €1,057.32

    Special Price €188.73

    SKU: RD34967-4X2.5L
    In Stock
  5. Merck

    Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L

    Regular Price: €74.20

    Special Price €20.61

    SKU: MC1060072500
    In Stock
  6. Applichem

    2-Propanol HPLC grade (for electrochemical detection),2,5 L

    €133.20
    SKU: AP221090.1612
    In Stock
  7. Applichem

    Acetonitrile (HPLC-gradient grade) PAI-ACS,1 L

    €77.20
    SKU: AP221881.1611
    In Stock
  8. Applichem

    Acetonitrile (HPLC-gradient grade) PAI-ACS,2,5 L

    Regular Price: €161.70

    Special Price €32.00

    SKU: AP221881.1612
    In Stock
  9. Applichem

    Acetone (UV-IR-HPLC-GPC) PAI-ACS,1 L

    €42.10
    SKU: AP361007.1611
    In Stock
  10. Applichem

    Acetone (UV-IR-HPLC-GPC) PAI-ACS,2,5 L

    €79.50
    SKU: AP361007.1612
    In Stock
  11. Applichem

    Acetic acid 100 % HPLC grade,1 L

    €44.90
    SKU: AP361008.1611
    In Stock
  12. Applichem

    2-Propanol (HPLC) PAI,1 L

    €62.52
    SKU: AP361090.1611
    In Stock
  13. Applichem

    2-Propanol (HPLC) PAI,2,5 L

    €122.28
    SKU: AP361090.1612
    In Stock
  14. Applichem

    Methanol (UV-IR-HPLC-HPLC isocratic) PAI-ACS,1 L

    €24.80
    SKU: AP361091.1611
    In Stock
  15. Applichem

    Methanol (UV-IR-HPLC-HPLC isocratic) PAI-ACS,2,5 L

    €46.60
    SKU: AP361091.1612
    In Stock
  16. Applichem

    Cyclohexane HPLC grade,2,5 L

    €140.30
    SKU: AP361250.1612
    In Stock
  17. Applichem

    Chloroform HPLC grade,2,5 L

    €89.30
    SKU: AP361252.1612
    In Stock
  18. Applichem

    Dichloromethane stabilized with ~20 ppm of amylene1 L

    €53.90
    SKU: AP361254.1611
    In Stock
  19. Applichem

    Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,1 L

    €106.60
    SKU: AP361736.1611
    In Stock
  20. Applichem

    Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L

    Regular Price: €221.40

    Special Price €199.26

    SKU: AP361736.1612
    In Stock
  21. Applichem

    N,N-Dimethylformamide HPLC grade,1 L

    €71.20
    SKU: AP361785.1611
    In Stock
  22. Applichem

    Acetonitrile (UV-IR-HPLC-isocratic) PAI-ACS,1 L

    €74.20
    SKU: AP361881.1611
    In Stock
  23. Applichem

    Acetonitrile (UV-IR-HPLC-isocratic) PAI-ACS,2,5 L

    Regular Price: €147.60

    Special Price €132.84

    SKU: AP361881.1612
    In Stock
  24. Applichem

    Dimethyl sulfoxide HPLC grade,1 L

    €82.60
    SKU: AP361954.1611
    In Stock
  25. Applichem

    n-Heptane (UV-IR-HPLC-HPLC preparative) PAI,2,5 L

    €187.10
    SKU: AP362062.1612
    In Stock
  26. Applichem

    Isooctane (UV-IR-HPLC) PAI-ACS,2,5 L

    €138.00
    SKU: AP362064.1612
    In Stock
  27. Applichem

    Diethyl ether HPLC grade,1 L

    €64.30
    SKU: AP362551.1611
  28. Applichem

    n-Hexane HPLC grade (95 % n-Hexan),2,5 L

    €113.40
    SKU: AP363242.1612
  29. Applichem

    Trifluoroacetic acid HPLC grade,25 ml

    €66.40
    SKU: AP363317.1606
    In Stock
  30. Applichem

    Tetrabutylammonium dihydrogen phosphate HPLC grade,25 g

    €569.40
    SKU: AP367038.1606
    In Stock
  31. Applichem

    Acetoxy valerenic acid HPLC grade,25 mg

    €565.00
    SKU: APA2051.0025
  32. Applichem

    Isoquercitrin HPLC grade,20 mg

    €329.00
    SKU: APA2064.0020
    In Stock
  33. Applichem

    Glucotropaeolin HPLC grade,50 mg

    €580.90
    SKU: APA5300.0050
    In Stock
  34. J.T. Baker

    Water, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L

    Regular Price: €36.72

    Special Price €24.75

    SKU: JB4218.2500
    In Stock
  35. J.T. Baker

    Methanol, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L

    Regular Price: €60.07

    Special Price €24.25

    SKU: JB8402.2500
    In Stock
  36. J.T. Baker

    Acetonitril, Baker HPLC Analyzed, Ultra Gradient HPLC Grade 2,5L

    Regular Price: €195.68

    Special Price €42.25

    SKU: JB9017.2500
    In Stock
  37. J.T. Baker

    2-Propanol (Iso-Propanol) by Baker HPLC, 2,5L glass bottle

    Regular Price: €132.78

    Special Price €76.49

    SKU: JB9095.2500
    In Stock
  38. Merck

    Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L

    Regular Price: €75.40

    Special Price €64.09

    SKU: MC1000142500
    In Stock
  39. Merck

    Acetonitrile hypergrade for LC-MS LiChrosolv, 2,5 L

    Regular Price: €269.00

    Special Price €57.00

    SKU: MC1000292500
    In Stock
  40. Merck

    Methanol hypergrade for LC-MS LiChrosolv, 2,5 L

    Regular Price: €76.40

    Special Price €36.27

    SKU: MC1060352500
    In Stock
  41. Merck

    2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L

    €166.80
    SKU: MC1096345000
    In Stock
  42. Merck

    Ethanol gradient grade for liquid chromatography LiChrosolv, 2,5 L

    Regular Price: €165.12

    Special Price €140.35

    SKU: MC1117272500
    In Stock
  43. Merck

    Water for chromatography LiChrosolv, 1 L

    €18.70
    SKU: MC1153331000
    In Stock
  44. J.T. Baker

    Methanol, Baker HPLC Analyzed, LC-MS Grade 2,5L

    Regular Price: €65.58

    Special Price €53.75

    SKU: JB9822.2500GL
    In Stock
  45. J.T. Baker

    Water for HPLC, LC-MS Grade, 1L glass bottle

    Regular Price: €35.65

    Special Price €30.04

    SKU: JB9825.1000GL
    In Stock
  46. Riedel-de Haen

    ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, Glass Bottle, 2.5 L

    Regular Price: €282.09

    Special Price €38.14

    SKU: RD34851-2.5L
  47. Riedel-de Haen

    Methanol G CHROMASOLV, gradient grade, for HPLC, ≥99.9%, Glass Bottle, 4 x 2.5 L

    Regular Price: €233.29

    Special Price €83.63

    SKU: RD34885-4X2.5L
    In Stock
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Information about the different purity levels for HPLC 

 For modern HPLC high purity solutions as mobile phases are increasingly needed. Our consumers can mainly choose between three different classes of purity for their individual analysis, HPLC grade, Gradient grade and LC-MS grade.

But whats the difference between the different qualities referring chromatography and between other high purity solvents? And why is it important to choose a solvent with reference to the performed application?

All qualities have the same basic material and a nominal high purity with low traces of xenobiotics (99.8 - 99.9 %, depending on the product) in common. But especially these minimal residue can cause problems in HPLC depending in the technic of detection, so that in this case "pure" is not equal to "pure".

The following describes the differences of the three levels of solvent purity and the reasons for using every quality for your application. 

HPLC grade

HPLC grade describes a solutions quality, that is mainly desinged for isocratic separations applying UV and fluorescence detection. These solvents are especially characerized by high purity combined with high UV permeability at all relevant wave lengths, low inherent fluorescence and waste steam pressure as well as low acidity and alkalinity.

The solvents undergo special treatment stages to remove potentially interferring substances. Thereby disturbing peaks are minimized and a low ambient noise can be enabled.

After the treatment these solutions are tested specificly for aptitude for fluorescence detection in HPLC. Through this quality controll continous purity of solvents as well as reproducability for your measurements can be guaranteed.

Gradient grade

For chromatographical separation in HPLC besides isocratic also gradient elutions are often applied. At these composition of mobile phase doesn´t change wuring measurement., so e.g. of reversed phase chromatography the organic part increases. Using solvents, that are not specially designed for gradient separation, can cause a distinct increasing of the base line at UV detection and can be sensitive for sensitivity of the method.

To ensure optimal chromatography taking gradient into account Gradient grade is further cleaned up. Besides suitability for UV and fluorescence detection all solutions are specially tested for applicability for gradiental separation, so that a charge consistent quality can be guaranteed for Gradient grade solutions.

The following figure shows a gradient profile to demonstrade the extraordinary results that can be attained with acetonitril Gradient grade. Only a low drift of the base line can be recorded.

Fig. 1: Drift of the base line using Gradient grade as solvent. 

LC-MS grade

With the success of (ultra) high pressure liquid chromatography mass spectroscopy (UHPLC-MS) many new requirements arised to the solvent used because of high detection sensitivity.

This way the focus by using mass spectroscopy lies especially on high ionisation efficience with concurrent low ion background. Therefore the solvents are applicable cleaned up. Thereby removing trace elements, organics and (metall) ions has first priority. These can cause an increased ambient noise and adduct formation with the substances, that should be analysed. The result is a decreased detection sensability.

For quality controll all solvents are tested versus Reserpin (m/z 609.49) as standard. The following figure shows the scan of a positiv and negativ ESI and APCi modus. The whole tested mass area displays a low noise ambient as well as no signals cause by impurities.

Fig. 2: Noise ambient of LC-MS measurements with LC-MS grade solvents.

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