TLC Plates

Thin-Layer Chromatography (TLC) Plates

The principle of thin-layer chromatography (TLC) has been known for over 100 years. TLC is still a very common and popular separation technique for qualitative and quantitative analysis in the laboratory. Like all chromatographic methods, the aim is to separate a compound into its chemical components. The success of this method is due to its simplicity, also in terms of sample preparation, low cost, versatility, fast development time, high sensitivity and excellent reproducibility. Thin-layer chromatography is also highly efficient: Up to 70 samples can be separated simultaneously on a TLC plate. TLC is used in many industries and research areas, e.g. clinical analysis, food chemistry and pharmaceutical production.

In our Analytics Shop you will find a large selection of TLC plates for your TLC analysis. TLC plates are available in a variety of materials like aluminium oxide, cellulose, diatomaceous earth, silica gel, Sil Gel, Silica Gel, with different pore sizes and modifications.

TLC accessories from all well-known manufacturers can also be found in the Analytics Shop. Please contact us if you have any questions on our products.

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Product Image of TLC-plates Nano-SIL C18-50, 10x10cm 25 pc/PAK
TLC-plates Nano-SIL C18-50, 10x10cm 25 pc/PAK
Item number: MN811054
Brand: Macherey-Nagel
€374.00
Product Image of TLC-plates Nano-SIL C18-100, 10x10cm, 25 pc/PAK
TLC-plates Nano-SIL C18-100, 10x10cm, 25 pc/PAK
Item number: MN811052
Brand: Macherey-Nagel
€374.00
Product Image of TLC-plates Nano-SIL PAH, 10x20cm, 50 pc/PAK
TLC-plates Nano-SIL PAH, 10x20cm, 50 pc/PAK
Item number: MN811051
Brand: Macherey-Nagel
€525.00
Product Image of TLC-plates Nano-SILGUR-20 UV254, 10x10cm, 25 pc/PAK
TLC-plates Nano-SILGUR-20 UV254, 10x10cm, 25 pc/PAK
Item number: MN811042
Brand: Macherey-Nagel
€238.00
Product Image of TLC-plates Nano-SILGUR-20, 10x10cm, 25 pc/PAK
TLC-plates Nano-SILGUR-20, 10x10cm, 25 pc/PAK
Item number: MN811032
Brand: Macherey-Nagel
€238.00
Product Image of TLC-plates Nano-SIL-20 UV254, 10x20cm, 50 pc/PAK
TLC-plates Nano-SIL-20 UV254, 10x20cm, 50 pc/PAK
Item number: MN811023
Brand: Macherey-Nagel
€432.00
Product Image of TLC-plates Nano-SIL-20 UV254, 10x10cm, 25 pc/PAK
TLC-plates Nano-SIL-20 UV254, 10x10cm, 25 pc/PAK
Item number: MN811022
Brand: Macherey-Nagel
€184.00
Product Image of TLC-plates Nano-SIL-20 UV254, 5x5cm, 100 pc/PAK
TLC-plates Nano-SIL-20 UV254, 5x5cm, 100 pc/PAK
Item number: MN811021
Brand: Macherey-Nagel
€241.00
Product Image of TLC-plates Nano-SIL-20, 10x20cm, 50 pc/PAK
TLC-plates Nano-SIL-20, 10x20cm, 50 pc/PAK
Item number: MN811013
Brand: Macherey-Nagel
€432.00
Product Image of TLC-plates Nano-SIL-20, 10x10cm, 25 pc/PAK
TLC-plates Nano-SIL-20, 10x10cm, 25 pc/PAK
Item number: MN811012
Brand: Macherey-Nagel
€184.00
Product Image of TLC-plates Nano-SIL-20, 5x5cm, 100 pc/PAK
TLC-plates Nano-SIL-20, 5x5cm, 100 pc/PAK
Item number: MN811011
Brand: Macherey-Nagel
€241.00
Product Image of TLC-plates SILGUR-25-C UV254, 20x20cm, 25 pc/PAK
TLC-plates SILGUR-25-C UV254, 20x20cm, 25 pc/PAK
Item number: MN810123
Brand: Macherey-Nagel
€249.00
Product Image of TLC-plates SIL G-25 TENSIDE, 20x20cm, 25 pc/PAK
TLC-plates SIL G-25 TENSIDE, 20x20cm, 25 pc/PAK
Item number: MN810063
Brand: Macherey-Nagel
€226.00
Product Image of TLC-plates SILCEL-Mix-25 UV254, 20x20cm, 25 pc/PAK
TLC-plates SILCEL-Mix-25 UV254, 20x20cm, 25 pc/PAK
Item number: MN810043
Brand: Macherey-Nagel
€266.00
Product Image of TLC-plates SILGUR-25 UV254, 20x20cm, 25 pc/PAK
TLC-plates SILGUR-25 UV254, 20x20cm, 25 pc/PAK
Item number: MN810023
Brand: Macherey-Nagel
€200.00
Product Image of TLC-plates SILGUR-25 UV254, 10x20cm, 50 pc/PAK
TLC-plates SILGUR-25 UV254, 10x20cm, 50 pc/PAK
Item number: MN810022
Brand: Macherey-Nagel
€249.00
Product Image of TLC-plates SILGUR-25, 20x20cm, 25 pc/PAK
TLC-plates SILGUR-25, 20x20cm, 25 pc/PAK
Item number: MN810013
Brand: Macherey-Nagel
€200.00
Product Image of TLC-plates SILGUR-25, 10x20cm 50 pc/PAK
TLC-plates SILGUR-25, 10x20cm 50 pc/PAK
Item number: MN810012
Brand: Macherey-Nagel
€249.00
Product Image of TLC-plates SIL G-25 UV 254+366, 20x20cm 25 pc/PAK
TLC-plates SIL G-25 UV 254+366, 20x20cm 25 pc/PAK
Item number: MN809123
Brand: Macherey-Nagel
€171.00
Product Image of TLC-plates SIL G-25 UV 254+366, 10x20cm 50 pc/PAK
TLC-plates SIL G-25 UV 254+366, 10x20cm 50 pc/PAK
Item number: MN809122
Brand: Macherey-Nagel
€203.00
Product Image of TLC-plates SIL G-25 UV 254+366, 5x20cm 100 pc/PAK
TLC-plates SIL G-25 UV 254+366, 5x20cm 100 pc/PAK
Item number: MN809121
Brand: Macherey-Nagel
€230.00
Product Image of TLC-plates SIL G-200 UV254, 20x20cm, 12 pc/PAK
TLC-plates SIL G-200 UV254, 20x20cm, 12 pc/PAK
Item number: MN809083
Brand: Macherey-Nagel
€269.00
Product Image of TLC-plates SIL G-200, 20x20cm 12 pc/PAK
TLC-plates SIL G-200, 20x20cm 12 pc/PAK
Item number: MN809073
Brand: Macherey-Nagel
€269.00
Product Image of TLC-plates SIL G-100 UV254, 20x20cm, 15 pc/PAK
TLC-plates SIL G-100 UV254, 20x20cm, 15 pc/PAK
Item number: MN809063
Brand: Macherey-Nagel
€249.00
Product Image of TLC-plates SIL G-100, 20x20cm, 15 pc/PAK
TLC-plates SIL G-100, 20x20cm, 15 pc/PAK
Item number: MN809061
Brand: Macherey-Nagel
€249.00
Product Image of TLC-plates SIL G-50 UV254, 20x20cm, 20 pc/PAK
TLC-plates SIL G-50 UV254, 20x20cm, 20 pc/PAK
Item number: MN809053
Brand: Macherey-Nagel
€238.00
Product Image of TLC-plates SIL G-50, 20x20cm, 20 pc/PAK
TLC-plates SIL G-50, 20x20cm, 20 pc/PAK
Item number: MN809051
Brand: Macherey-Nagel
€238.00
Product Image of TLC-plates SIL G-25 HR/UV254, 20x20cm, 25 pc/PAK
TLC-plates SIL G-25 HR/UV254, 20x20cm, 25 pc/PAK
Item number: MN809043
Brand: Macherey-Nagel
€204.00
Product Image of TLC-plates SIL G-25 HR, 20x20cm, 25 pc/PAK
TLC-plates SIL G-25 HR, 20x20cm, 25 pc/PAK
Item number: MN809033
Brand: Macherey-Nagel
€204.00
Product Image of TLC-plates SIL G-25 UV254, 2,5x7,5cm, 100 pc/PAK
TLC-plates SIL G-25 UV254, 2,5x7,5cm, 100 pc/PAK
Item number: MN809028.100
Brand: Macherey-Nagel
€206.00
Product Image of TLC-plates SIL G-25 UV254, 5x10cm, 200 pc/PAK
TLC-plates SIL G-25 UV254, 5x10cm, 200 pc/PAK
Item number: MN809027.200
Brand: Macherey-Nagel
€301.00
Product Image of TLC-plates SIL G-25 UV254, 5x10cm, 50 pc/PAK
TLC-plates SIL G-25 UV254, 5x10cm, 50 pc/PAK
Item number: MN809027
Brand: Macherey-Nagel
€139.00
Product Image of TLC-plates SIL G-25 UV254, 20x20cm, 25 pc/PAK
TLC-plates SIL G-25 UV254, 20x20cm, 25 pc/PAK
Item number: MN809023
Brand: Macherey-Nagel
€181.00
Product Image of TLC-plates SIL G-25 UV254, 10x20cm, 50 pc/PAK
TLC-plates SIL G-25 UV254, 10x20cm, 50 pc/PAK
Item number: MN809022
Brand: Macherey-Nagel
€203.00
Product Image of TLC-plates SIL G-25 UV254, 5x20cm, 100 pc/PAK
TLC-plates SIL G-25 UV254, 5x20cm, 100 pc/PAK
Item number: MN809021
Brand: Macherey-Nagel
€230.00
Product Image of TLC-plates SIL G-25 UV254, 10x10cm, 25 pc/PAK
TLC-plates SIL G-25 UV254, 10x10cm, 25 pc/PAK
Item number: MN809020
Brand: Macherey-Nagel
€76.70
Product Image of TLC-plates SIL G-25, 5x10cm, 200 pc/PAK
TLC-plates SIL G-25, 5x10cm, 200 pc/PAK
Item number: MN809017.200
Brand: Macherey-Nagel
€301.00
Product Image of TLC-plates SIL G-25, 5x10cm, 50 pc/PAK
TLC-plates SIL G-25, 5x10cm, 50 pc/PAK
Item number: MN809017
Brand: Macherey-Nagel
€139.00
Product Image of TLC-plates SIL G-25, 20x20cm, 25 pc/PAK
TLC-plates SIL G-25, 20x20cm, 25 pc/PAK
Item number: MN809013
Brand: Macherey-Nagel
€181.00
Product Image of TLC-plates SIL G-25, 10x20cm, 50 pc/PAK
TLC-plates SIL G-25, 10x20cm, 50 pc/PAK
Item number: MN809012
Brand: Macherey-Nagel
€203.00
Product Image of TLC-plates SIL G-25, 5x20cm, 100 pc/PAK
TLC-plates SIL G-25, 5x20cm, 100 pc/PAK
Item number: MN809011
Brand: Macherey-Nagel
€230.00
Product Image of TLC-plates CEL 400-10, 20x20cm, 25 pc/PAK
TLC-plates CEL 400-10, 20x20cm, 25 pc/PAK
Item number: MN808073
Brand: Macherey-Nagel
€184.00
Product Image of TLC-plates CEL 400-10, 10x20cm, 50 pc/PAK
TLC-plates CEL 400-10, 10x20cm, 50 pc/PAK
Item number: MN808072
Brand: Macherey-Nagel
€256.00
Product Image of TLC-plates CEL 300-50 UV254, 20x20cm, 20 pc/PAK
TLC-plates CEL 300-50 UV254, 20x20cm, 20 pc/PAK
Item number: MN808063
Brand: Macherey-Nagel
€308.00
Product Image of TLC-plates CEL 300-50, 20x20cm, 20 pc/PAK
TLC-plates CEL 300-50, 20x20cm, 20 pc/PAK
Item number: MN808053
Brand: Macherey-Nagel
€308.00
Product Image of TLC-plates CEL 300-25 UV254, 20x20cm, 25 pc/PAK
TLC-plates CEL 300-25 UV254, 20x20cm, 25 pc/PAK
Item number: MN808043
Brand: Macherey-Nagel
€308.00
Product Image of TLC-plates CEL 300-25, 20x20cm, 25 pc/PAK
TLC-plates CEL 300-25, 20x20cm, 25 pc/PAK
Item number: MN808033
Brand: Macherey-Nagel
€308.00
Product Image of TLC-plates CEL 300-10 UV254, 20x20cm, 25 pc/PAK
TLC-plates CEL 300-10 UV254, 20x20cm, 25 pc/PAK
Item number: MN808023
Brand: Macherey-Nagel
€184.00
Product Image of TLC-plates CEL 300-10, 20x20cm, 25 pc/PAK
TLC-plates CEL 300-10, 20x20cm, 25 pc/PAK
Item number: MN808013
Brand: Macherey-Nagel
€184.00
Product Image of TLC-plates ALOX-100 UV254, 20x20cm, 15 pc/PAK
TLC-plates ALOX-100 UV254, 20x20cm, 15 pc/PAK
Item number: MN807033
Brand: Macherey-Nagel
€243.00

Selection of the Solvent
The solvent is selected according to elution strength. With the help of a quick test you can test different solvents and find the suitable one. 

Sample Application
It is common to use a glass capillary as a dot or short line. Too wide substance zones during application lead to poor separation, as the zones become even wider during separation. After application, the solvent is allowed to evaporate, which can be accelerated by using a dryer.

Development
A common method is to use a development chamber. The eluent is poured into the bottom of the chamber.  The saturation of the chamber with the solvent vapour is necessary for a reproducible running distance. The TLC plate with the sample is then positioned in the chamber. The separation is complete when the eluent has risen to the upper end of the TLC plate. Now the TLC plate can be removed and dried accordingly.

Evaluation
The evaluation depends on the aim of the chromatographic analysis. For qualitative determinations it is easiest to run comparison substances. Quantitative evaluations are possible via corresponding calibration measurements. The area of the substance spots is used or a photometric evaluation is carried out on the layer. However, the latter requires more equipment.