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The method of photometry is frequently used to determine the exact concentration of colored solutions. This application of light measurement is based on the principle of attenuation of the light on passing through the sample solution. The spectrum of light reaches wavelengths between 390 and 770 nanometers. The extent of the subsequent light attenuation is, under specified conditions, a calculation factor for determining the concentration. Generally when applying the photometric method either infrared or ultraviolet light is used.

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Photometry

 

Basics of photometry

Quantitative analysis

The main application purpose of photometry takes place when the concentration of sample solutions is to be determined. This method is based on the Lambert-Beer's law, which describes the relationship between the absorbance of the sample at a certain wavelength and the concentration thereof. The measurement of the concentration of dissolved substances is designed to be as quickly and relatively simple as possible.

Qualitative analysis

The term of qualitative analysis is described as a so-called identity resolution. It is used less frequently and has a not quite as high significance as the quantitative analysis. Not many substances show characteristic absorption maxima and thus release information on their purity under certain conditions. As quick reference here is the analysis of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) - the photometry is used regularly for this purpose.