HPLC Column Viva Biphenyl, 300Å, 5 µm, 50 x 2.1 mm

Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.

Since 2005, the Restek Biphenyl has offered a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention — especially for dipolar, unsaturated, or conjugated solutes — and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses.