SPME

SPME (Solid Phase Micro Extraction) is a procedure of sampling. Later the samples can be analyzed via GC or HPLC. The sampling by SPME, which no solvent, was developed and patented by the chemist Janusz Pawlizyn. Marketing is carried out by the only licensee SUPELCO, which was part of the Sigma-Aldrich group and is now part of Merck.

For more Information about the SPME and coating materials, please click here.

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Product Image of Bruker/Varian 1078/1079 Inlet liners, SPME 0.75 mm ID, length 54 mm, 5 pc/pak
Bruker/Varian 1078/1079 Inlet liners, SPME 0.75 mm ID, length 54 mm, 5 pc/pak
Item number: SG092117
Brand: Trajan (SGE)
€175.90
Product Image of Combo PK of SPME Fibers Fiber Length of 10mm
Combo PK of SPME Fibers Fiber Length of 10mm
Item number: RE27483
Brand: Restek
€888.00
Product Image of Merlin Microseal High Pressure Replacement Septum
Merlin Microseal High Pressure Replacement Septum
Item number: RE22782
Brand: Restek
€248.00
Product Image of Merlin Microseal Adapter Kit for Varian 1177 SPME System
Merlin Microseal Adapter Kit for Varian 1177 SPME System
Item number: RE22780
Brand: Restek
€712.00
Product Image of Merlin Microseal Adapter Kit for Varian 1078 and 1079 SPME Systems
Merlin Microseal Adapter Kit for Varian 1078 and 1079 SPME Systems
Item number: RE22779
Brand: Restek
€674.00
Product Image of SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs Siltek Deact., 5/PAK
SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs Siltek Deact., 5/PAK
Item number: RE22279-214.5
Brand: Restek
€224.00
Product Image of SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs Base Deact., 5/PAK
SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs Base Deact., 5/PAK
Item number: RE22279-210.5
Brand: Restek
€164.00
Product Image of SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs IP Deact., 5/PAK
SPME Liner 0.75mm x 5.0 x 95 for Shimadzu GCs IP Deact., 5/PAK
Item number: RE22279
Brand: Restek
€147.00
Product Image of SPME Liner 0.75mm x 6.30 x 74 for Varian GCs IP Deact., 5/PAK
SPME Liner 0.75mm x 6.30 x 74 for Varian GCs IP Deact., 5/PAK
Item number: RE21113
Brand: Restek
€220.00
Product Image of SPME Liner 0.75mm x 6.35 x 78.5 for Agilent GCs IP Deact., 5/PAK
SPME Liner 0.75mm x 6.35 x 78.5 for Agilent GCs IP Deact., 5/PAK
Item number: RE21111
Brand: Restek
€193.00
Product Image of Liner, Ultra Inert, straight, wool, 0.8 mm, Varian/Bruiker, for SPME, 5 pc/PAK
Liner, Ultra Inert, straight, wool, 0.8 mm, Varian/Bruiker, for SPME, 5 pc/PAK
Item number: AG8004-0176
Brand: Agilent Technologies
€187.00
Product Image of SPME Fiber Assembly PEG, Smart, 60 µm, 23 Gauge, for PAL 3
SPME Fiber Assembly PEG, Smart, 60 µm, 23 Gauge, for PAL 3
Item number: SU548676-U
Brand: Supelco
€268.71
Product Image of SPME Fiber Assembly Carboxen/PDMS, Smart, 85 µm, 23 Gauge, for PAL 3
SPME Fiber Assembly Carboxen/PDMS, Smart, 85 µm, 23 Gauge, for PAL 3
Item number: SU548551-U
Brand: Supelco
€268.71
Product Image of Supel BioSPME 96-Pin Device, C18, 10 pc/PAK
Supel BioSPME 96-Pin Device, C18, 10 pc/PAK
Item number: SU59683-U
Brand: Supelco
€4,458.75
Product Image of Supel BioSPME 96-Pin Device, C18
Supel BioSPME 96-Pin Device, C18
Item number: SU59680-U
Brand: Supelco
€646.29
Product Image of Supel BioSPME 96-Pin Device Positioning Adapter
Supel BioSPME 96-Pin Device Positioning Adapter
Item number: SU59686-U
Brand: Supelco
€611.49
Product Image of SMART SPME Arrow 1.5 mm: PDMS phase thickness 250 µm, phase length 20 mm, black, 3 pc/PAK
SMART SPME Arrow 1.5 mm: PDMS phase thickness 250 µm, phase length 20 mm, black, 3 pc/PAK
Item number: TF36SA25P3-SM
Brand: Thermo Fisher Scientific
€1,775.18
Product Image of SMART SPME Arrow 1.5 mm: PDMS, phase thickness 250 µm, phase length 20 mm, black
SMART SPME Arrow 1.5 mm: PDMS, phase thickness 250 µm, phase length 20 mm, black
Item number: TF36SA25P1-SM
Brand: Thermo Fisher Scientific
€637.24
Product Image of SMART SPME Arrow 1.10 mm: DVB/PDMS, phase thickness 120 µm, phase length 20 mm, purple, 3 pc/PAK
SMART SPME Arrow 1.10 mm: DVB/PDMS, phase thickness 120 µm, phase length 20 mm, purple, 3 pc/PAK
Item number: TF36SA12E3-SM
Brand: Thermo Fisher Scientific
€1,480.00
Product Image of SMART SPME Arrow 1.10 mm: Carbon WR/PDMS, phase thickness 120 µm, phase length 20 mm, light blue, 3 pc/pack
SMART SPME Arrow 1.10 mm: Carbon WR/PDMS, phase thickness 120 µm, phase length 20 mm, light blue, 3 pc/pack
Item number: TF36SA12B3-SM
Brand: Thermo Fisher Scientific
€1,480.00
Product Image of SMART SPME Arrow 1.10 mm: Carbon WR/PDMS, phase thickness 120 µm, phase length 20 mm, light blue
SMART SPME Arrow 1.10 mm: Carbon WR/PDMS, phase thickness 120 µm, phase length 20 mm, light blue
Item number: TF36SA12B1-SM
Brand: Thermo Fisher Scientific
€531.02
Product Image of SMART SPME Arrow 1.10 mm: DVB/C-WR/PDMS, phase thickness 120 µm, phase length 20 mm, dark gray, 3 pc/PAK
SMART SPME Arrow 1.10 mm: DVB/C-WR/PDMS, phase thickness 120 µm, phase length 20 mm, dark gray, 3 pc/PAK
Item number: TF36SA11T3-SM
Brand: Thermo Fisher Scientific
€1,480.00
Product Image of SMART SPME Arrow 1.10 mm: DVB/C-WR/PDMS, phase thickness 120 µm, phase length 20 mm, dark gray
SMART SPME Arrow 1.10 mm: DVB/C-WR/PDMS, phase thickness 120 µm, phase length 20 mm, dark gray
Item number: TF36SA11T1-SM
Brand: Thermo Fisher Scientific
€531.02
Product Image of SMART SPME Arrow 1.10 mm: PDMS, phase thickness 100 µm, phase length 20 mm, red, 3 pc/PAK
SMART SPME Arrow 1.10 mm: PDMS, phase thickness 100 µm, phase length 20 mm, red, 3 pc/PAK
Item number: TF36SA10P3-SM
Brand: Thermo Fisher Scientific
€1,480.00
Product Image of SMART SPME Arrow 1.10 mm: PDMS, phase thickness 100 µm, phase length 20 mm, red
SMART SPME Arrow 1.10 mm: PDMS, phase thickness 100 µm, phase length 20 mm, red
Item number: TF36SA10P1-SM
Brand: Thermo Fisher Scientific
€531.02
Product Image of SMART SPME Arrows 1.10 mm SET, 5 St/Set
SMART SPME Arrows 1.10 mm SET, 5 St/Set
Item number: TF36SA10M5-SM
Brand: Thermo Fisher Scientific
€2,577.93
Product Image of SMART SPME Arrow 1.10 mm: acrylate, phase thickness 100 µm, phase length 20 mm, grey
SMART SPME Arrow 1.10 mm: acrylate, phase thickness 100 µm, phase length 20 mm, grey
Item number: TF36SA10A1-SM
Brand: Thermo Fisher Scientific
€531.02
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 100µm, Fiber Length 10mm, Red, 3 pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 100µm, Fiber Length 10mm, Red, 3 pc/PAK
Item number: TF36SP10P3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 100µm, Fiber Length 10mm, Red, 1 pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 100µm, Fiber Length 10mm, Red, 1 pc/PAK
Item number: TF36SP10P1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of Collection Of Five Different Smart SPME Fibers, Length Of 10mm, Fiber Thickness of PDMS, 100mm DVB/PDMS 65mm Carbon WR/PDMS 95mm Acrylate 85mm DVB/Carbon WR/PDMS 50/30 µm
Collection Of Five Different Smart SPME Fibers, Length Of 10mm, Fiber Thickness of PDMS, 100mm DVB/PDMS 65mm Carbon WR/PDMS 95mm Acrylate 85mm DVB/Carbon WR/PDMS 50/30 µm
Item number: TF36SP08M5-SM
Brand: Thermo Fisher Scientific
€1,488.74
Product Image of Smart SPME Fiber, DVB / PDMS (Divinylbenzene / Polydimethylsiloxane), Fiber Thickness 65µm, Fiber Length 10Mm, Violet, 3pc/PAK
Smart SPME Fiber, DVB / PDMS (Divinylbenzene / Polydimethylsiloxane), Fiber Thickness 65µm, Fiber Length 10Mm, Violet, 3pc/PAK
Item number: TF36SP06E3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Smart SPME Fiber, DVB / PDMS (Divinylbenzene / Polydimethylsiloxane), Fiber Thickness 65µm, Fiber Length 10Mm, Violet, 1pc/PAK
Smart SPME Fiber, DVB / PDMS (Divinylbenzene / Polydimethylsiloxane), Fiber Thickness 65µm, Fiber Length 10Mm, Violet, 1pc/PAK
Item number: TF36SP06E1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of Smart SPME Fiber, DVB / Carbon WR / PDMS (Divinylbenzene / Carbon Wide Range / Polydimethylsiloxane), Fiber Thickness 50/30µm, Fiber Length 10mm, Dark Gray, 3pc/PAK
Smart SPME Fiber, DVB / Carbon WR / PDMS (Divinylbenzene / Carbon Wide Range / Polydimethylsiloxane), Fiber Thickness 50/30µm, Fiber Length 10mm, Dark Gray, 3pc/PAK
Item number: TF36SP05T3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Smart SPME Fiber, DVB / Carbon WR / PDMS (Divinylbenzene / Carbon Wide Range / Polydimethylsiloxane), Fiber Thickness 50/30µm, Fiber Length 10mm, Dark Gray,  1 pc/PAK
Smart SPME Fiber, DVB / Carbon WR / PDMS (Divinylbenzene / Carbon Wide Range / Polydimethylsiloxane), Fiber Thickness 50/30µm, Fiber Length 10mm, Dark Gray, 1 pc/PAK
Item number: TF36SP05T1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 30µm, Fiber Length 10mm, Golden, 3 pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 30µm, Fiber Length 10mm, Golden, 3 pc/PAK
Item number: TF36SP03P3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 30µm, Fiber Length 10mm, Golden, 1 pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 30µm, Fiber Length 10mm, Golden, 1 pc/PAK
Item number: TF36SP03P1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 7µm, Fiber Length 10mm, Green, 3 pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane) Fiber Thickness 7µm, Fiber Length 10mm, Green, 3 pc/PAK
Item number: TF36SP01P3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Smart SPME Fiber, PDMS (Polydimethylsiloxane), Fiber Thickness 7µm, Fiber Length 10mm, Green,  1pc/PAK
Smart SPME Fiber, PDMS (Polydimethylsiloxane), Fiber Thickness 7µm, Fiber Length 10mm, Green, 1pc/PAK
Item number: TF36SP01P1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of Conversion Kit with 1.1mm Merlin Microseal Kit for Split/Splitless Injector Agilent 8890 Split/Splitless Injector
Conversion Kit with 1.1mm Merlin Microseal Kit for Split/Splitless Injector Agilent 8890 Split/Splitless Injector
Item number: RE28934
Brand: Restek
€3,220.00
Product Image of Split/splitless weldment and septum nut, for Agilent 8890 Split/Splitless Injector, Modified for Arrow
Split/splitless weldment and septum nut, for Agilent 8890 Split/Splitless Injector, Modified for Arrow
Item number: RE28933
Brand: Restek
€3,000.00
Product Image of Vial, screw top, headspace, Clear, Round Bottom, 10 mL, 23 x 46 mm
Vial, screw top, headspace, Clear, Round Bottom, 10 mL, 23 x 46 mm
Item number: AG5188-5392
Brand: Agilent Technologies
€78.00
Product Image of Liner, UI, straight 0.75 mm ID 25pc/PAK
Liner, UI, straight 0.75 mm ID 25pc/PAK
Item number: AG5190-7045
Brand: Agilent Technologies
€872.00
Product Image of Liner,  Ultra Inert, straight, 0.75 mm ID, 5 pc/PAK
Liner, Ultra Inert, straight, 0.75 mm ID, 5 pc/PAK
Item number: AG5190-4056
Brand: Agilent Technologies
€201.00
Product Image of Liner, GC, Ultra Inert, straight, 0.75 mm ID, recommended for SPME injections
Liner, GC, Ultra Inert, straight, 0.75 mm ID, recommended for SPME injections
Item number: AG5190-4048
Brand: Agilent Technologies
€54.00
Product Image of SMART SPME Arrows Carbon WR, phase thickness 95µm, phase length 10 mm, darkblue, 1 pc/Pak
SMART SPME Arrows Carbon WR, phase thickness 95µm, phase length 10 mm, darkblue, 1 pc/Pak
Item number: TF36SP09C1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 85µm, phase length 10 mm, grey, 3 pc/Pak
SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 85µm, phase length 10 mm, grey, 3 pc/Pak
Item number: TF36SP08A3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 85µm, phase length 10 mm, grey, 1 pc/Pak
SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 85µm, phase length 10 mm, grey, 1 pc/Pak
Item number: TF36SP08A1-SM
Brand: Thermo Fisher Scientific
€316.36
Product Image of SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 100µm, phase length 20 mm, grey, 3 pc/Pak
SMART SPME Arrows Acrylate (Polyacrylate), phase thickness 100µm, phase length 20 mm, grey, 3 pc/Pak
Item number: TF36SA10A3-SM
Brand: Thermo Fisher Scientific
€1,480.00
Product Image of Smart SPME-Arrow, 1.5 mm, 120 µm DVB/CarbonWR/PDMS, 3 pc/PAK
Smart SPME-Arrow, 1.5 mm, 120 µm DVB/CarbonWR/PDMS, 3 pc/PAK
Item number: AG5610-5864
Brand: Agilent Technologies
€2,244.00
Product Image of Smart SPME-Arrow, 1.1 mm, 120 µm DVB/CarbonWR/PDMS, 3 pc/PAK
Smart SPME-Arrow, 1.1 mm, 120 µm DVB/CarbonWR/PDMS, 3 pc/PAK
Item number: AG5610-5861
Brand: Agilent Technologies
€1,871.00

Samples which are to be analyzed by gas chromatography are usually filled in so-called autosampler vials, which are sealed with a crimp lid. For sampling, an SPME needle is pierced through the septum into the sample vial. An SPME needle looks similar to a normal cannula because it is also made of stainless steel. However, this does not serve to remove or inject fluids, but helps in penetrating the septum. There is a fused silica (fused silica) or glass fiber (StableFlex) in the needle, coated on the end. This coated fiber absorbs analyte molecules from the sample. The sample can either be taken directly from the liquid phase or from the gas phase above the liquid, the so-called head-space. If the sample is to be taken out of the gas phase, a volatility of the analyte has to be given, so that enrichment from the gas phase is possible. More information about Headspace analysis can be found here.

Desorption

Following the extraction of the analyte from the liquid sample, is the desorption in order to release the analytes from the coated fiber of the SPME needle. Different methods are suitable depending on the choice of the desired analytical method. If sample is getting analyzed by of gas chromatography, the desorption can be carried out without further intermediate steps within the sample inlet of the gas chromatograph. In this case, the so called thermal desorption is used, using the high temperature within the chromatograph to dissolve the absorbed analyte molecules from the fiber and to convert them into the gas phase. The steps of thermal desorption of the analytes and transfer into the gas phase for the gas-chromatographic separation are connected and thus analyte loss is avoided or at least minimized. The temperatures of the Inlet depend on the respective temperature during the chromatographic separation, but usually are between 100 and 300 ° C.

The absorbed analyte molecules can also be dissolved from the fiber with the aid of a solvent. This method is used if later analysis is to be carried out by HPLC (high pressure liquid chromatography).

Coating materials

Through the use of different coating materials, substance groups can be selectively absorbed. The commercially available adsorbing materials which differ greatly in their polarity include: carbowax, polydivinylbenzene, polyacrylate, carboxes and PDMS (polydimethylsiloxane). Depending on the analyte, combinations of different layer thicknesses and adsorbing materials are used.

The following table lists the fibers recommended by Merck for various types of analytes (MW = molar mass):

Analyt Type Recommended Fiber
Gases and low molecular weight compounds (MW 30-225) 75 µm/85 µm Carboxen/polydimethylsiloxane
Volatiles (MW 60-275) 100 µm polydimethylsiloxane
Volatiles, amines and nitro-aromatic compounds (MW 50-300) 65 µm polydimethylsiloxane/divinylbenzene
Polar semi-volatiles (MW 80-300) 85 µm polyacrylate
Non-polar high molecular weight compounds (MW 125-600) 7 µm polydimethylsiloxane
Non-polar semi-volatiles (MW 80-500) 30 µm polydimethylsiloxane
Alcohols and polar compounds (MW 40-275) 60 µm Carbowax (PEG)
Flavor compounds: volatiles and semi-volatiles, C3-C20 (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber
Trace compound analysis (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a 2 cm StableFlex fiber
Amines and polar compounds (HPLC use only) 60 µm polydimethylsiloxane/divinylbenzene

Advantages of SPME:

  • Solvent-free micro-extraction technology for almost all samples and matrices
  • easy to automate
  • compatible with GC
  • reusable
  • inexpensive
  • fast
  • does not destroy the sample

 

For further information on SPME we recommend the How to get started guide by Merck!

Here you can go back to the products.