Cells for Fluorescence Spectroscopy

Production Informations for Cells made by Hellma:

Ultrasonic Machinig:

Many cell typed, especially flow-trough cells, require borings and cavities with highly complex shapes. With ultrasonic machining, which resembles the spark erosion procedure used in metall working, Hellma possesses a technology that allows to produce high-precision borings and cavities of 0,5 nm to 60 nm in the brittle glass material. The tools required for manufacturing borings of almost any imaginable cross-sectional shape are made in Hellma´s own machine-too, department.

Fusion Technology:

the process of direct fusion, which was developed by Hellma, and is almost always used nowadays ideally fulfills these requirements. However, it also presupposes that the surfaces to be fused together are polished and possess a flatness tolerance of less than 1 µm. Since no adhesives whatsoever are employed for fusing the glass parts, the seams display the same high resistance against chemicals and increased temperatures as the solid glass itself. Sinter glass fusing is only used in the few exceptional cases where direct fusion is impossible for technical manufacturing reasons.

Antireflection Coating:

When radiation passes through the cell, part of it is reflected by the exterior surfaces. The transmission is reduced by this reflected portion by approximately 8%. Vacuum evaporation of thin layers of a suitable material can reduce these reflections, thereby giving a higher transmission for the cell.
The standard is a multilayer antireflection coating that reduces reflection over a broad spectral range. Over a spectral range of between 440 nm and 650 nm the reduced reflectance is at most 0,4%. This coating is highly adhesive and resistant to both abrasion and climatic influences.

Mirror Coating:

For some purposes (e.g. fluorescence measurements) mirror coated cells are used. Both the rear window to the incident light and the left adjacent window are mirror-coated on the outside. The standard mirror coating consists of a layer of vacuum-evaporated aluminium that has a degree of reflection of mor than 80% over a spectral range between 250 nm and 2500 nm. This coating is covered with a protective layer which is extremly hard and durable. Additionally, the mirror coated window are protected against external scratches by a layer of black lacquer.
On request we can also supply metal coatings with different reflective properties and with the coated surfaces in other positions.

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