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In addition to regular maintenance of the HPLC and UHPLC system it is recommended to use an inline-filter to protect the column and the plant. We offer  inline-filters of world-known manufacturers such as Agilent Technologies, Vici Jour and Upchurch Scientific.To keep HPLC and UHPLC systems free of contaminants that can damage the analytical column or even the entire system, the highest purity for all solutions that come into direct contact with the plant is necessary.  The housing of the inline-filter is made of PEEK or stainless steel with a frit in the interior and standard fittings at both ends. There are inline-filters, which are completely replaced if necessary, or a variant in which only the frit must be replaced.

If you have any questions while choosing a product, feel free to contact us.

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HPLC Inline-Filter

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  1. Upchurch Scientific

    Precolumn Filter, PEEK, 2µm, 1pc/PAK

    sku: UPA-355
  2. Upchurch Scientific

    VHP Replacement Filter, 0.2µm, 1 pc/PAK

    sku: UPVHP-506
  3. Waters

    Filter (Last Drop)W, 2µm SS

    sku: WTPSL901290
  4. Waters

    Filter (Last Drop)W, 10µm SS

    sku: WTPSL901291
  5. Waters

    Filter (Last Drop)W, 2µm PAT

    sku: WTPSL901292
  6. Waters

    Filter (Last Drop)W, 5µm PAT

    sku: WTPSL901294
  7. Waters

    In-Line precolumn Filter kit

    sku: WTWAT084560
  8. Waters

    Precolumn Filter Insert Assembly

    sku: WTWAT088084
  9. Upchurch Scientific

    Precolumn Filter Assy 2µm, 1pc/PAK

    sku: UPA-315
  10. Upchurch Scientific

    SemiPrep Inline Filter Assembly, 2µm, 1pc/PAK

    sku: UPA-410
  11. Upchurch Scientific

    Inline Filter Assembly, 0.5µm, 1pc/PAK

    sku: UPA-431
  12. Upchurch Scientific

    VHP Inline Filter, 0.2µm, 1pc/PAK

    sku: UPVHP-505
  13. Supelco

    Inline Filter, stainless steel, 0,2 µm

    sku: SU52246-U
  14. Supelco

    Inline Filter, 2 µm, high pressure

    sku: SU55078
  15. Supelco

    Inline Filter, 0,5 µm, high pressure

    sku: SU55079
  16. Supelco

    Inline Filter, check valve

    sku: SU55085-U
  17. Upchurch Scientific

    Precolumn Filter, 0.5µm, 1,3µl swept volume, 1pc/PAK

    sku: UPA-316
  18. Upchurch Scientific

    Precolumn Filter, 0.5µm, 0, 84µl swept volume, 1pc/PAK

    sku: UPA-318
  19. Upchurch Scientific

    SemiPrep Filter Assembly, 10 µm, 1pc/PAK

    sku: UPA-330
  20. Upchurch Scientific

    Precolumn Filter, PEEK, 0.5µm, 1pc/PAK

    sku: UPA-356
  21. Upchurch Scientific

    SemiPrep Filter Assembly, 10µm, 1pc/PAK

    sku: UPA-360
  22. Upchurch Scientific

    SemiPrep Inline Filter Assembly, 10µm, 1pc/PAK

    sku: UPA-411
  23. Upchurch Scientific

    Low Pressure Inline Filter, 2 µm, for 1/16'' OD

    sku: UPA-415
  24. Upchurch Scientific

    Inline Filter Assembly, 2µm, 1pc/PAK

    sku: UPA-430
  25. Upchurch Scientific

    SemiPrep Filter, 5µm, for 3/16'' OD, 1pc/PAK

    sku: UPA-510
  26. Upchurch Scientific

    NanoFilter Capsule, 1µm, Titaniumfrit, 2 pc/PAK

    sku: UPM-126
  27. Upchurch Scientific

    Precolumn MicroFilter Assembly, 1pc/PAK

    sku: UPM-510
  28. Upchurch Scientific

    Mini MicroFilter Assembly, 1µm, 5 capsules, 1pc/PAK

    sku: UPM-533
  29. Upchurch Scientific

    Precolumn MicroFilter, 0.5µm, with 5 SSfrits, 1pc/PAK

    sku: UPM-550
  30. Upchurch Scientific

    Stainless Steel Conductive Capsule 1µm, 2 pc/PAK

    sku: UPM-128
  31. Agilent Technologies

    Inline Filter, stainless steel, LC-2010/20AD/AB/AT SHM

    sku: AG8001-0808
  32. Upchurch Scientific

    Inline Filter, metal free, 2µm, for 1/16'' OD, 1pc/PAK

    sku: UPA-424
  33. Upchurch Scientific

    Inline MicroFilter Assembly, with 5 fittings, 1pc/PAK

    sku: UPM-520
  34. Upchurch Scientific

    Inline MicroFilter Kit, PEEK, 0.5µm, for 1/32'' OD

    sku: UPM-525
  35. PerkinElmer

    Inlet HPLC Filter, 2 µm

    sku: PE09923405
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HPLC inline filters for your laboratory

Use of inline filters in chromatography

Impurities can not only get into the system from outside but also from the system itself. The main sources are mainly the piston seals the pump or the rotor seal the injection valve, which can produce abrasion with increasing operating time. This abrasion can lead to deposits / blockage on the stationary phase or in the HPLC system.

In addition to regular maintenance of the system it is recommended to use an inline filter to protect the column and the plant. But what is the inline filter and why makes its use a lot of sense? And what is important in choosing the right inline filter?

The in-line filter can be positioned at various locations of the HPLC system. If the inline filter is installed between the pump and the injector, particles and impurities are removed from the eluent. Likewise, abrasion of the piston seal is intercepted, so that the rotor seal of the injection valve is protected from abrasion and fine particles. When positioning directly in front of the column after the injection valve and contaminants from the sample are removed and abrasion of the rotor seals.

In addition to the inline filter it is usually recommended to use a guard column. Molecules that can be attached irreversibly to the main column can not be retained with the inline filter. Inline filters are available in various designs. In addition to housing, which is either made of PEEK or stainless steel can also choose between different fittings. These depend on the place where the inline filter should be installed. Thus, there are also housing, which are provided for the direct connection to the analytical column.

Accessories for the inline filters: frits

The frits for the inline filters are usually made of stainless steel and are often surrounded by a ring of PEEK. This serves as a seal ring, while minimizing the dead volume of the inline filter, so that no negative effects such as peak broadening can be expected. Stainless steel frits can be used for the current analysis. For applications requiring high corrosion resistance and biocompatibility, frits made of other materials such as Titanium or polymers are available. Also, the surrounding ring can be made of other materials, such as of PTFE. The user then has the possibility to choose a matching inline filter depending on the sample to be analyzed and the chemical properties of the mobile phase.

Typical pore sizes of the frits are 2 and 0.5 microns. When using columns with a particle size of <5 micron, frits with 0.5 microns should be used. For UHPLC systems and columns with particle sizes below 2 microns using frits with a pore size of 0.2 microns is recommended. The maximum pressure is 1000 bar and they have a low dead volume.

In the long term, the use of inline filters in the HPLC analysis has many advantages and saves money. Maintenance costs will be reduced and the facility and the separation column with usage of the inline filter will guarantee an extended service life.