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In addition to regular maintenance of the HPLC and UHPLC system it is recommended to use an inline-filter to protect the column and the plant. We offer  inline-filters of world-known manufacturers such as Agilent Technologies, Vici Jour and Upchurch Scientific.To keep HPLC and UHPLC systems free of contaminants that can damage the analytical column or even the entire system, the highest purity for all solutions that come into direct contact with the plant is necessary.  The housing of the inline-filter is made of PEEK or stainless steel with a frit in the interior and standard fittings at both ends. There are inline-filters, which are completely replaced if necessary, or a variant in which only the frit must be replaced.

If you have any questions while choosing a product, feel free to contact us.

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HPLC Inline-Filter

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  1. Upchurch Scientific

    Inline MicroFilter Assembly 1µm, Stainless Steel- 5/pk Single

    Regular Price: $146.24

    Special Price $138.93

    sku: UPM-135-1
  2. Upchurch Scientific

    Stainless Steel Inline Solvent Filter w/20µm Frit

    Regular Price: $91.86

    Special Price $87.27

    sku: UPA-313
  3. Upchurch Scientific

    Inline MicroFilter Assembly 5 Fittings

    Regular Price: $157.28

    Special Price $149.42

    sku: UPM-520
  4. Upchurch Scientific

    Inline VHP Filter 0.2µm

    Regular Price: $469.52

    Special Price $446.04

    sku: UPVHP-505
  5. Agilent Technologies

    Inline Filter, stainless steel, 0,2 µm, 4,6 mm, for RRLC, con. capill.

    Regular Price: $333.06

    Special Price $316.41

    sku: AG5067-1553
  6. Vici Jour

    Sure-Guard, disposable In-Line Filter, TI frit 0.5 um

    Regular Price: $22.74

    Special Price $21.60

    sku: JR-0611-TI05
  7. Upchurch Scientific

    Inline Filter, solvent filter, female end

    Regular Price: $47.69

    Special Price $45.31

    sku: UPA-314-01
  8. Agilent Technologies

    Inline Filter, stainless steel, 0,2 µm, 2,1 mm, for RRLC, con. capill.

    Regular Price: $261.32

    Special Price $248.25

    sku: AG5067-1551
  9. Agilent Technologies

    Inline Filter, stainless steel, LC-2010/20AD/AB/AT SHM

    Regular Price: $137.07

    Special Price $130.22

    sku: AG8001-0808
  10. Upchurch Scientific

    Stainless Steel Inline Solvent Filter, 2µm

    Regular Price: $91.86

    Special Price $87.27

    sku: UPA-314
  11. Upchurch Scientific

    Inline Filter Metal Free 2µm, for 1/16'' OD

    Regular Price: $33.96

    Special Price $32.26

    sku: UPA-424
  12. Upchurch Scientific

    Inline Filter Metal Free 10µm, for 1/16'' OD

    Regular Price: $30.68

    Special Price $29.15

    sku: UPA-425
  13. Upchurch Scientific

    Inline MicroFilter Kit PEEK 0.5µm

    Regular Price: $173.06

    Special Price $164.41

    sku: UPM-525
  14. QLA

    Inline Filter, housing assembly

    Regular Price: $99.53

    Special Price $94.55

    sku: QLFILASY-DK
  15. SGE

    LC-ILFF IN-LINE FILTER

    Regular Price: $41.81

    Special Price $39.72

    sku: SG202009
  16. Agilent Technologies

    Inline Filter, 2 µm, low dispersion

    Regular Price: $228.02

    Special Price $216.62

    sku: AG01090-68702
  17. Agilent Technologies

    Inline Filter, Universal-Kit: 2 frits, frit holder, connection capill.

    Regular Price: $288.23

    Special Price $273.82

    sku: AG01090-68703
  18. Agilent Technologies

    Inline Filter, 0,5 µm, u-LC

    Regular Price: $252.36

    Special Price $239.74

    sku: AG5064-8273
  19. Agilent Technologies

    Inline Filter, glass, 10 µm, kit

    Regular Price: $1,055.54

    Special Price $1,002.76

    sku: AG5065-4500
  20. Agilent Technologies

    In-line SS filter for G1311A

    Regular Price: $393.27

    Special Price $373.61

    sku: AGG1311-60006
  21. Upchurch Scientific

    Inline Filter, body .006in thru

    Regular Price: $52.82

    Special Price $50.18

    sku: UPM-520-01
  22. Upchurch Scientific

    Inline Filter, solvent filter, male end

    Regular Price: $39.93

    Special Price $37.93

    sku: UPA-314-02
  23. Vici Jour

    Sure-Guard, disposable In-Line Filter,SS frit 0.5 um

    Regular Price: $23.87

    Special Price $22.68

    sku: JR-0611-SS05
  24. Vici Jour

    Sure-Guard, disposable In-Line Filter,SS frit 2.0 um

    Regular Price: $23.87

    Special Price $22.68

    sku: JR-0611-SS2
  25. Vici Jour

    Sure-Guard, disposable In-Line Filter, TI frit 2.0 um

    Regular Price: $22.74

    Special Price $21.60

    sku: JR-0611-TI2
  26. Vici Jour

    Filter, PEEK, In-Line, complete, frit Ti PEEK-encased 5 um

    Regular Price: $74.46

    Special Price $70.74

    sku: JR-68256
  27. Vici Jour

    Filter, SS, In-Line ID 0.25 mm, complete, frit SS 0.5 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68330-05
  28. Vici Jour

    Filter, SS, In-Line ID 0.25 mm, complete, frit SS 2 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68330-2
  29. Vici Jour

    Filter, SS, In-Line ID 0.25 mm, complete, frit SS 5 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68330-5
  30. Vici Jour

    Filter, SS, In-Line ID 0.75 mm, complete, frit SS 0.5 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68331-05
  31. Vici Jour

    Filter, SS, In-Line ID 0.75 mm, complete, frit SS 2 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68331-2
  32. Vici Jour

    Filter, SS, In-Line ID 0.75 mm, complete, frit SS 5 um, with nuts and ferrules

    Regular Price: $126.50

    Special Price $120.18

    sku: JR-68331-5
  33. Agilent Technologies

    Inline Filter, 0,5 µm, PEEK, kit

    Regular Price: $444.51

    Special Price $422.28

    sku: AG5067-1582
  34. Agilent Technologies

    Inline Filter, pressure relief valve kit

    Regular Price: $1,509.02

    Special Price $1,433.57

    sku: AGG4212-68001
  35. Agilent Technologies

    Inline Filter, 1-M-120

    Regular Price: $197.27

    Special Price $187.41

    sku: AGUCM520
  36. Agilent Technologies

    Quat. Pump In-Line Filter upgrade kit

    Regular Price: $261.32

    Special Price $248.25

    sku: AGG7104-68000
  37. Vici Jour

    Sure-Guard, disposable In-Line Filter, SS frit 0.5 um, Waters type

    Regular Price: $36.19

    Special Price $34.38

    sku: JR-0641-SS05
  38. Vici Jour

    Sure-Guard, disposable In-Line Filter, SS frit 2 um, Waters type

    Regular Price: $36.19

    Special Price $34.38

    sku: JR-0641-SS2
  39. Vici Jour

    Sure-Guard, disposable In-Line Filter, Ti frit 0.5 um, Waters type

    Regular Price: $36.19

    Special Price $34.38

    sku: JR-0641-TI05
  40. Vici Jour

    Sure-Guard, disposable In-Line Filter, Ti frit 2 um, Waters type

    Regular Price: $34.43

    Special Price $32.71

    sku: JR-0641-TI2
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HPLC inline filters for your laboratory

Use of inline filters in chromatography

Impurities can not only get into the system from outside but also from the system itself. The main sources are mainly the piston seals the pump or the rotor seal the injection valve, which can produce abrasion with increasing operating time. This abrasion can lead to deposits / blockage on the stationary phase or in the HPLC system.

In addition to regular maintenance of the system it is recommended to use an inline filter to protect the column and the plant. But what is the inline filter and why makes its use a lot of sense? And what is important in choosing the right inline filter?

The in-line filter can be positioned at various locations of the HPLC system. If the inline filter is installed between the pump and the injector, particles and impurities are removed from the eluent. Likewise, abrasion of the piston seal is intercepted, so that the rotor seal of the injection valve is protected from abrasion and fine particles. When positioning directly in front of the column after the injection valve and contaminants from the sample are removed and abrasion of the rotor seals.

In addition to the inline filter it is usually recommended to use a guard column. Molecules that can be attached irreversibly to the main column can not be retained with the inline filter. Inline filters are available in various designs. In addition to housing, which is either made of PEEK or stainless steel can also choose between different fittings. These depend on the place where the inline filter should be installed. Thus, there are also housing, which are provided for the direct connection to the analytical column.

Accessories for the inline filters: frits

The frits for the inline filters are usually made of stainless steel and are often surrounded by a ring of PEEK. This serves as a seal ring, while minimizing the dead volume of the inline filter, so that no negative effects such as peak broadening can be expected. Stainless steel frits can be used for the current analysis. For applications requiring high corrosion resistance and biocompatibility, frits made of other materials such as Titanium or polymers are available. Also, the surrounding ring can be made of other materials, such as of PTFE. The user then has the possibility to choose a matching inline filter depending on the sample to be analyzed and the chemical properties of the mobile phase.

Typical pore sizes of the frits are 2 and 0.5 microns. When using columns with a particle size of <5 micron, frits with 0.5 microns should be used. For UHPLC systems and columns with particle sizes below 2 microns using frits with a pore size of 0.2 microns is recommended. The maximum pressure is 1000 bar and they have a low dead volume.

In the long term, the use of inline filters in the HPLC analysis has many advantages and saves money. Maintenance costs will be reduced and the facility and the separation column with usage of the inline filter will guarantee an extended service life.