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Chromatography
HPLC Columns
Hilicon HPLC Columns

Hilicon HPLC Columns

product category

Package specification The packing specification describes the material of the stationary phase. The name indicates whether a functional group is bound to the carrier material (silica gel, polymer) and, if so, which functional group. Depending on the bound functional group (e.g. C18 chain), this results in the separation mode of the column (reversed phase).

mode

Length Depending on the type of application or system, different lengths of HPLC columns are used. The length of the column together with the internal diameter defines the column volume. For preparative separations, for example, columns from 250 mm in length with a larger internal diameter are often used; this allows more material to be separated in one run. If you have any questions about suitable HPLC column dimensions for your analysis, please do not hesitate to contact us.

Inner diameter In addition to the length of the column, the inner diameter (ID) determines the column volume. The larger the internal diameter, the larger the volume and the higher the consumption of solvent. The inner diameter also influences the concentration of the analyte in the detector. For analyses where only a small amount of sample is available, it is advisable to use the smallest possible inner diameter. If an existing method is optimized from a larger to a smaller inner diameter, it must be ensured that the flow is adjusted for a comparable separation so that a constant linear velocity u is obtained

Particle Size The choice of particle size for the column depends initially on the HPLC system used. Particle sizes <2µm can be used with a UHPLC. In an HPLC system, which can operate at pressures of up to 400 bar, particles of 5 µm are commonly used. The smaller the particle in the column, the higher the resulting back pressure in the HPLC system.

Pore size

pH Range HPLC columns have a certain pH stability. The manufacturer usually specifies a recommended pH value range for a particular column at which the column can be operated in the long term. A pH value >7 is considered critical for silica gels, as they gradually dissolve. This dissolution is slowed down by a special polymer coating or the introduction of hybrid material. If a pH value >8 is used during the routine, it is advisable to use a polymer-based material, for example.

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Product Image of HPLC Column iHILIC-(P) Classic, HILIC, SS, 200 Å, 5 µm, 3 x 100 mm

HPLC Column iHILIC-(P) Classic, HILIC, SS, 200 Å, 5 µm, 3 x 100 mm

Item number: HIL164-103-0510

Brand: Hilicon

$1,281.28

Excl. 0% VAT

Items 151-151 of 151

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