HPLC Column Configurator

Find and compare HPLC Columns with our HPLC Column Configurator

With the help of our Liquid Chromatography Column Configurator, you can easily find the HPLC column you are looking for. Choose from over 130,000 HPLC columns from more than 30 quality manufacturers and brands, such as Agilent Technologies, YMC, Grace, Chiral, Machery-Nagel and many more. In addition, we offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative.

By using the drop-down menus on the left, you can easily select the column description, packing material, particle size and other specifications as well as the desired manufacturer. If you are unable to find a suitable HPLC column or have any questions, please feel free to contact us.

For use in regulated environments, our HPLC columns are labeled with the universal USP code. Further information on official methods and HPLC column comparisons can be found on the United States Pharmacopeia (USP) website. Furthermore, we have a list of USP column suggestions which can be downloaded here.

Sort By

Items 1-50 of 103583

per page
Product Image of Column Storage Cabinet, 30 columns (up to 300mm length)
Column Storage Cabinet, 30 columns (up to 300mm length)
Item number: AAColStore30
Brand: Altmann Analytik

In stock

$482.07 $535.63
Product Image of HPLC Column Symmetry C18 100Å 5µm 4.6x250mm
HPLC Column Symmetry C18 100Å 5µm 4.6x250mm
Item number: WTWAT054275
Brand: Waters
$1,160.00
Product Image of HPLC column CHIRALPAK AGP, 100 x 3 mm, 5 µm
HPLC column CHIRALPAK AGP, 100 x 3 mm, 5 µm
Item number: DAIC30783
Brand: Chiral
$1,850.00
Product Image of HPLC Column NUCLEODUR 100-5 C18 ec 250x4,6mm
HPLC Column NUCLEODUR 100-5 C18 ec 250x4,6mm
Item number: MN760002.46
Brand: Macherey-Nagel

In stock

$605.00
Product Image of HPLC Column Xbridge C18 130Å 250 mm 4.6 mm 5 µm, 1 - 12pH Range, Low Silan. Act., 185 m2/g, EndCapped, Spher., 18% Carb. Load
HPLC Column Xbridge C18 130Å 250 mm 4.6 mm 5 µm, 1 - 12pH Range, Low Silan. Act., 185 m2/g, EndCapped, Spher., 18% Carb. Load
Item number: WT186003117
Brand: Waters
$1,260.00
Product Image of HPLC Column Kromasil 100-5-C8 4.6 x 250 mm
HPLC Column Kromasil 100-5-C8 4.6 x 250 mm
Item number: KSM05CMA25
Brand: Kromasil

In stock

$534.00
Product Image of HPLC Column Hibar 250x4.6mm, Purospher STAR RP-18 endcapped, 5µm
HPLC Column Hibar 250x4.6mm, Purospher STAR RP-18 endcapped, 5µm
Item number: MC1514560001
Brand: Merck

In stock

$1,462.28
Product Image of HPLC Column Primesep A 2.1x50mm 5 µm 100 Å
HPLC Column Primesep A 2.1x50mm 5 µm 100 Å
Item number: SLA-21.050.0510
Brand: SIELC

In stock

$1,400.40
Product Image of HPLC Column YMC-Pack ODS-AQ, 12 nm, S-5µm, 250 x 4.6 mm
HPLC Column YMC-Pack ODS-AQ, 12 nm, S-5µm, 250 x 4.6 mm
Item number: YMAQ12S05-2546WT
Brand: YMC
$969.00
Product Image of HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm
Item number: SU53829-U
Brand: Supelco

In stock

$1,332.00
Product Image of HPLC Column Nucleosil 100 C18, 5.0 µm, 4.6 x 250 mm, 15% Carbon, endcapped
HPLC Column Nucleosil 100 C18, 5.0 µm, 4.6 x 250 mm, 15% Carbon, endcapped
Item number: AANNS1C9-5025046
Brand: Altmann Analytik

In stock

$560.00
Product Image of HPLC Column Asahipak ODP-50 4E, 250 Å, 5 µm, 4.6 x 250 mm
HPLC Column Asahipak ODP-50 4E, 250 Å, 5 µm, 4.6 x 250 mm
Item number: SDF7620003
Brand: Shodex
$1,052.00
Product Image of HPLC Column Reprosil Chiral-AMS, 1000Å, 10.0 µm, 4.6 x 250 mm
HPLC Column Reprosil Chiral-AMS, 1000Å, 10.0 µm, 4.6 x 250 mm
Item number: AANRSCHMS-10025046
Brand: Dr. Maisch

In stock

$1,290.00
Product Image of HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 250 mm, 12% Carbon
HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 250 mm, 12% Carbon
Item number: AANLI6RS-5025040
Brand: Altmann Analytik

In stock

$630.00
Product Image of HPLC Column CHIRALPAK® AD-3, 250 x 4,6 mm, 3 µm
HPLC Column CHIRALPAK® AD-3, 250 x 4,6 mm, 3 µm
Item number: DAIC19525
Brand: Chiral
$1,710.00
Product Image of HPLC Column ACE 3 C18 150x4.6mm
HPLC Column ACE 3 C18 150x4.6mm
Item number: ACACE-111-1546
Brand: ACE

In stock

$1,105.00
Product Image of HPLC Column Poroshell 120 EC-C18, 2,7 µm, 3 x 100 mm
HPLC Column Poroshell 120 EC-C18, 2,7 µm, 3 x 100 mm
Item number: AG695975-302
Brand: Agilent Technologies
$868.00
Product Image of HPLC Column EC 125/4.6 NUCLEOSIL 100-5 C18
HPLC Column EC 125/4.6 NUCLEOSIL 100-5 C18
Item number: MN720002.46
Brand: Macherey-Nagel
$625.00
Product Image of HPLC Column ZIC-HILIC 150x4.6mm, 3.5µm, 100Å PEEK HPLC Column SeQuant(TM)
HPLC Column ZIC-HILIC 150x4.6mm, 3.5µm, 100Å PEEK HPLC Column SeQuant(TM)
Item number: MC1504440001
Brand: Merck

In stock

$1,536.00
Product Image of HPLC Column Nucleosil 100 C18, 5.0 µm, 4 x 125 mm, 15% Carbon, endcapped
HPLC Column Nucleosil 100 C18, 5.0 µm, 4 x 125 mm, 15% Carbon, endcapped
Item number: AANNS1C9-5012540
Brand: Altmann Analytik

In stock

$406.00
Product Image of HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4.6 x 125 mm, 12% Carbon
HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4.6 x 125 mm, 12% Carbon
Item number: AANLI6RS-5012546
Brand: Altmann Analytik

In stock

$511.00
Product Image of HPLC Column Hypersil ODS, 120Å, 5.0 µm, 4.6 x 250 mm, 10% Carbon, endcapped
HPLC Column Hypersil ODS, 120Å, 5.0 µm, 4.6 x 250 mm, 10% Carbon, endcapped
Item number: AANHSO-5025046
Brand: Altmann Analytik

In stock

$655.20
Product Image of HPLC Column Hypersil BDS C18, 120Å, 5.0 µm, 4.6 x 100 mm, 11% Carbon, endcapped
HPLC Column Hypersil BDS C18, 120Å, 5.0 µm, 4.6 x 100 mm, 11% Carbon, endcapped
Item number: AANHSBC9-5010046
Brand: Altmann Analytik

In stock

$582.00
Product Image of HPLC Column Superspher RP18-endc., 100Å, 4.0 µm, 4 x 125 mm, 21,5% Carbon, endcapped
HPLC Column Superspher RP18-endc., 100Å, 4.0 µm, 4 x 125 mm, 21,5% Carbon, endcapped
Item number: AANSUR9E-4012540
Brand: Altmann Analytik

In stock

$768.00
Product Image of HPLC Column Spherisorb ODS-2, 80Å, 5.0 µm, 4 x 150 mm, 12% Carbon, endcapped
HPLC Column Spherisorb ODS-2, 80Å, 5.0 µm, 4 x 150 mm, 12% Carbon, endcapped
Item number: AANSSO2-5015040
Brand: Altmann Analytik

In stock

$453.60
Product Image of HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 125 mm, 12% Carbon
HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 125 mm, 12% Carbon
Item number: AANLI6RS-5012540
Brand: Altmann Analytik

In stock

$462.00
Product Image of HPLC Column Lichrospher 100 RP18, 5.0 µm, 4 x 125 mm, 21% Carbon
HPLC Column Lichrospher 100 RP18, 5.0 µm, 4 x 125 mm, 21% Carbon
Item number: AANLI1R9-5012540
Brand: Altmann Analytik

In stock

$418.60
Product Image of Universal Reversed Phase (RP) PreColumn Cartridge 5x2mm for Columns with 2 mm ID, 5/PAK
Universal Reversed Phase (RP) PreColumn Cartridge 5x2mm for Columns with 2 mm ID, 5/PAK
Item number: AAVRP-500520-5
Brand: Altmann Analytik

In stock

$280.00
Product Image of Universal Reversed Phase (RP) PreColumn Cartridge 20x4mm for Columns with 3, 4 and 4,6 mm ID, 5/PAK
Universal Reversed Phase (RP) PreColumn Cartridge 20x4mm for Columns with 3, 4 and 4,6 mm ID, 5/PAK
Item number: AAVRP-201040-5
Brand: Altmann Analytik

In stock

$330.00
Product Image of HPLC Guard Column Nucleosil 100 C18, 5.0 µm, 4 x 10 mm, 5pc/PAK
HPLC Guard Column Nucleosil 100 C18, 5.0 µm, 4 x 10 mm, 5pc/PAK
Item number: AAVNS1C9-501040-5
Brand: Altmann Analytik

In stock

$240.00
Product Image of HPLC Column Superspher RP18, 100Å, 4.0 µm, 4.6 x 300 mm, 21% Carbon
HPLC Column Superspher RP18, 100Å, 4.0 µm, 4.6 x 300 mm, 21% Carbon
Item number: AANSUR9-4030046
Brand: Altmann Analytik

In stock

$1,352.00
Product Image of HPLC Column Ultrasep ES Pharm-RP8, 100Å, 5.0 µm, 3 x 250 mm
HPLC Column Ultrasep ES Pharm-RP8, 100Å, 5.0 µm, 3 x 250 mm
Item number: AANUSEPR8E-5025030
Brand: Altmann Analytik

In stock

$468.00
Product Image of HPLC Column Repromer 1000 RPS, 8.0 µm, 4.6 x 250 mm, polymer phase with PSDVB matrix
HPLC Column Repromer 1000 RPS, 8.0 µm, 4.6 x 250 mm, polymer phase with PSDVB matrix
Item number: AANRM0RS-8025046
Brand: Altmann Analytik

In stock

$840.00
Product Image of HPLC Column Hypersil BDS C18, 120Å, 5.0 µm, 4.6 x 33 mm, 11% Carbon, endcapped
HPLC Column Hypersil BDS C18, 120Å, 5.0 µm, 4.6 x 33 mm, 11% Carbon, endcapped
Item number: AANHSBC9-503346
Brand: Altmann Analytik

In stock

$480.00
Product Image of HPLC Column Equisil BDS C8, 120Å, 5.0 µm, 4.6 x 33 mm, 7% Carbon, endcapped
HPLC Column Equisil BDS C8, 120Å, 5.0 µm, 4.6 x 33 mm, 7% Carbon, endcapped
Item number: AANEQBC8-503346
Brand: Altmann Analytik

In stock

$228.00
Product Image of HPLC Column Peerless Basic 100 C18EC, 5.0 µm, 4.6 x 150 mm
HPLC Column Peerless Basic 100 C18EC, 5.0 µm, 4.6 x 150 mm
Item number: AANPLB1C9-5015046
Brand: Altmann Analytik

In stock

Product Image of HPLC Column Hypersil Chiral-ZT, 5 µm, 4.6 x 250 mm
HPLC Column Hypersil Chiral-ZT, 5 µm, 4.6 x 250 mm
Item number: TF43605-254530
Brand: Thermo Fisher Scientific
$2,027.00
Product Image of HPLC Guard MaxPeak Premier Protein SEC, 250Å, 2.5 µm, 4.6 x 30 mm
HPLC Guard MaxPeak Premier Protein SEC, 250Å, 2.5 µm, 4.6 x 30 mm
Item number: WT186009969
Brand: Waters
$420.00
Product Image of HPLC Column Genesis 4µm Phenyl 150x3.9 mm
HPLC Column Genesis 4µm Phenyl 150x3.9 mm
Item number: GRGEN-4PH-150DE
Brand: Avantor

In stock

$780.00
Product Image of HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 4.6 x 150 mm
HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 4.6 x 150 mm
Item number: WT186010334
Brand: Waters
$1,480.00
Product Image of HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 4.6 x 50 mm
HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 4.6 x 50 mm
Item number: WT186010332
Brand: Waters
$1,040.00
Product Image of HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 2.1 x 100 mm
HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 2.1 x 100 mm
Item number: WT186010330
Brand: Waters
$1,270.00
Product Image of HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 2.1 x 50 mm
HPLC Column XBridge Premier Protein BEH C4 300 Å, 2.5 µm, 2.1 x 50 mm
Item number: WT186010329
Brand: Waters
$1,040.00
Product Image of U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 150 mm
U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 150 mm
Item number: WT186010328
Brand: Waters
$1,590.00
Product Image of U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 100 mm
U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 100 mm
Item number: WT186010327
Brand: Waters
$1,480.00
Product Image of U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 50 mm
U(H)PLC Column ACQUITY Premier Protein BEH C4 300 Å, 1.7 µm, 2.1 x 50 mm
Item number: WT186010326
Brand: Waters
$1,380.00
Product Image of U(H)PLC Column ACQUITY Premier Protein SEC, 250 Å, 1.7 µm, 4.6 x 300 mm
U(H)PLC Column ACQUITY Premier Protein SEC, 250 Å, 1.7 µm, 4.6 x 300 mm
Item number: WT186009964
Brand: Waters
$4,300.00
Product Image of U(H)PLC Column ACQUITY Premier Protein SEC, 250 Å, 1.7 µm, 4.6 x 150 mm
U(H)PLC Column ACQUITY Premier Protein SEC, 250 Å, 1.7 µm, 4.6 x 150 mm
Item number: WT186009963
Brand: Waters
$3,000.00
Product Image of HPLC Column XBridge Premier Protein SEC,  250Å, 2.5 µm, 7.8 x 150 mm
HPLC Column XBridge Premier Protein SEC, 250Å, 2.5 µm, 7.8 x 150 mm
Item number: WT186009961
Brand: Waters
$2,700.00
Product Image of HPLC Column XBridge Premier Protein SEC,  250Å, 2.5 µm, 4.6 x 300 mm
HPLC Column XBridge Premier Protein SEC, 250Å, 2.5 µm, 4.6 x 300 mm
Item number: WT186009960
Brand: Waters
$2,800.00

HPLC columns for every application

Find the right column from over 130,000 HPLC columns from more than 30 different manufacturers. We also offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative. Compare prices and the application possibilities of the various manufacturers. If you are unable to find a suitable HPLC column, please feel free to contact us.

Prior to the development of the Reversed Phase (RP), Normal Phase Chromatography was the most common separation mode. We offer Normal Phase (NP) HPLC columns by all well-known manufacturers here.

In Normal Phase Chromatography, the mobile phase is non-polar and the stationary phase is polar (common: silica gel). This technique relies on the interaction of analytes with polar functional groups on the surface of the stationary phase. This interaction is strongest when using non-polar solvents as the mobile phase. The least polar compounds elute first and the most polar compounds elute last.

Normal Phase Chromatography is a very effective separation method because a wide range of solvents can be used to fine-tune the selectivity of a separation. However, it has become less popular with many chromatographers because of the complexity involved. Under certain conditions, long equilibration times or reproducibility problems may occur. This is due mainly to the sensitivity of the technique to the presence of low concentrations of polar contaminants in the mobile phase. Controlling these problems effectively produces better chromatograms than reverse phase methods as the commonly used solvents have a lower viscosity.

HPLC columns for Reversed Phase (RP) Chromatography

In Reversed Phase Chromatography, the polarity of the phases are "reversed". The mobile phase is polar and the stationary phase is non-polar. As it is one of the most popular separation modes in chromatography, all related products of all well-known manufacturers are available here.

The non-polar side chains in Reversed Phase Chromatography columns are bound either to a polymer or to a structure made of silica gel, which leads to them being hydrophobic. The longer the chain, the more non-polar the phases are. Polar analytes are eluted from the column first, followed by the non-polar analytes. Reversed phase is more widely practised than normal phased as they can be used universally for polar and non-polar analytes. In addition, this method is very sensitive and flexible as small changes in the composition of the mobile phase (e.g. salts, pH, organic solvents) or the temperature can completely change the separation properties of the system. RP-HPLC is used especially in numerous applications of UV spectroscopy (LC-UV).

Chiral columns for Chiral/Enantiomeric chromatography

Unlike normal HPLC columns, chiral or enantiomeric HPLC can also be used to separate and determine chiral compounds. This requires special chiral stationary phases that have fixed chiral functionalities. In the Analytics Shop, we have over 1,400 different chiral columns from different manufacturers, including the high-quality columns from Chiral Technologies as well as lower-priced alternatives of the same quality from YMC and Dr. Maisch.

Special columns such as chiral or enantiomeric HPLC columns are required to separate chiral compounds. With suitable interaction, these enable the separation of the enantiomers which have almost identical physical and chemical properties. In the simplest case, an enantiomer has only one chiral center. In larger molecules, however, there are often enantiomers with several chiral centers, which must then all have the "opposite" configuration In contrast, diastereomers have at least one chiral center that is the same and at least one that is different in configuration. Diasteromers usually differ in their properties and can therefore often be better separated. Therefore, sometimes a diastereomer is synthesized from an enantiomer to achieve (better) separation. A conventional solvent is used as the eluent.

UHPLC and UPLC® columns

With Ultra High Performance Liquid Chromatography (UHPLC), dramatic increases in resolution, speed and sensitivity of HPLC can be achieved by using short and thin columns. The particles of the filling material have diameters of less than 2 µm, which results in an improved separation performance compared to standard HPLC. The increased total surface area of the filling material gives the analyte a higher adsorption ability. Analysis times are reduced due to the shorter columns and less solvent is required.

Columns for Hydrophilic Interaction Chromatography (HILIC)

Hydrophilic interaction chromatography (HILIC) is a popular alternative to normal phase and reverse phase chromatography. Similar to NP, in the HILIC method, polar stationary phases are used, but with mobile phases comparable to those used in RP chromatography. The mobile phase typically consists of a high organic content (usually acetonitrile) and a low proportion of aqueous solvent/buffer or another polar solvent. In the HILIC method, water is the strongest eluent. A HILIC column is particularly suitable for separating highly polar substances such as carbohydrates, amino acids, bases, and alkaloids. An extensive range of HILIC columns from established quality manufacturer YMC is available in our shop.

Columns for Supercritical Fluid Chromatography (SFC)

Significant advantages of Supercritical Liquid Chromatography (SFC) columns compared to HPLC columns:

  1. Inexpensive: reduced solvent comsumption
  2. Environmentally friendly
  3. Time-saving: quick separation with high resolution
  4. Flexible: chiral and achiral phases

Supercritical fluids show low viscosities and higher diffusivities when used as a mobile phase, resulting in narrower peaks due to rapid diffusion and faster elution and less pressure drop through the column. Supercritical fluids combine the benefits of liquids and gases, hence enabling the SFC technique to combine the best aspects of HPLC and gas chromatography (GC). In most cases, a supercritical fluid such as carbon dioxide is used as the mobile phase. The low viscosity of supercritical carbon dioxide enables analytical separations that are 3-5 times faster than those for normal phase HPLC. The speed of SFC separations, the preservation of organic solvents and more concentrated product fractions make SFC a preferred chromatographic technique for separating and purifying chemical mixtures.

The use of high-performance preparative columns (internal diameter of 10 - 50 mm) with a large number of particle sizes from 3 - 20 μm leads to the quick separation and recovery of cleaned components.

Nano HPLC columns

Nano HPLC uses columns with very small inner diameters. Columns with internal diameters of 75 µm, 100 µm or 150 µm are commonly used. Due to the reduction in the internal diameter, injection and flow rates must be reduced which is particularly advantageous when only small or diluted sample quantities are available. The smaller nano HPLC columns lead to an increased sensitivity with lower solvent consumption. Nano columns are able to maintain a high concentration of the injected sample and to direct approx. 40-50 % of the sample to the detector. Nano HPLC has a high separation efficiency compared to the traditional HPLC technique.

Columns for Gel Permeation Chromatography (GPC) & Size Exclusion Chromatography (SEC)

GPC/SEC (Gel Permeation / Size Exclusion Chromatography) is a separation method in which the analytes are separated on the basis of their size, or rather their hydrodynamic volume. GPC/SEC columns are packed with very small porous beads. The smaller molecules can enter the pores more easily and therefore spend more time in these pores, eluting last. Conversely, larger molecules spend little if any time in the pores and are eluted quickly. The GPC columns are filled with a microporous packing material and gel, hence the name gel permeation.

Ion Exchange Chromatography (IEX)

Ion exchange chromatography (IEX), today often referred to simply as ion chromatography (IC), separates molecules based on the respective charged groups on the surface of a protein. IC is used for the separation or purification of proteins and antibodies. However, it is also popular for oligonucleotides, sugar or RNA molecules, etc. The main advantages of IC are speed, sensitivity, selectivity and simultaneity. It should be noted that, depending on the separation column, only cations or anions can be separated.

The pH value, the concentration or the gradient are important aspects affecting the results of IEC.

The stationary phase usually consists of a polymer resin such as polystyrene, cellulose, cross-linked polyacrylamide or polydextran. A distinction is made between strong and weak anion or cation exchangers.

Permitted changes according to USP (United States Pharmacopeia)

The U.S. Pharmacopeia Convention is a scientific non-profit company that sets the standards for ingredients, concentration, quality and purity of medicines, food ingredients and food supplements that are manufactured, distributed and consumed all over the world. According to USP regulations, the following deviations may occur:

USP Chapter

USP Chapter Values
Column Length ± 70 %
Column Inner Diameter can be changed if the flow rate is kept constant.
Particle Size - 50 %
Flow Rate ± 50 %
Ratio of components
in the mobile phase
± 30 % or ± 10 %
pH value of the mobile phase ± 0,2
Salt concentration in the buffer ± 10 %
Column Temperature ± 10° C
Wave Length of the UV-Vis detector ± 3 nm
Injection Volume can be reduced until precision and detection limits are reached.


Permitted changes according to EP (European Pharmacopeia)

European Pharmacopeia Ph. Eur., Chapter 2.2.46

The European Pharmacopeia is a published collection of monographs describing the individual and general quality standards of ingredients, dosages and analytical methods of medicine. The aim is to set common quality standards across Europe to control the quality of medicines and other chemical products. According to the EP regulation, the following deviations can occur:

Isocratic elution

Isocratic elution Values
Column Length ± 70 %
Column Inner Diameter ± 25 %
Particle Size - 50 %
Flow Rate ± 50 %
Ratio to components
in the mobile phase
± 30% or ± 2% absolute
pH value of the mobile phase ± 0,2
Salt concentration in the buffer ± 10 %
Column Temperature ± 10° C
Wave Lenght of the detector ± 3 nm
Injection Volume can be reduced until precision and detection limits are reached.



Gradient elution

Gradient elution Values
Column Length ± 70 %
Column Inner Diameter ± 25 %
Particle Size no changes permitted
Flow Rate changes acceptable if column size is changed.
Ratio to components
in the mobile phase
small changes in the composition of the mobile phase and the (density) grade are acceptable if the system configuration still meets the requirements.
Dwell Volume gradient instants can be used to compensate for differences in dwell volume between different systems.
pH value of the mobile phase no changes permitted
Salt concentration in the buffer no changes permitted
Column Temperature ± 5° C
Wave Lenght of the detector no deviations permitted
Injection Volume can be reduced until precision and detection limits are reached.


HPLC column recommendations according to USP

Phase Name USP Number Column Recommendations
Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size L1 Potential Columns
Porous silica gel, 5 to 10 μm particle size L3 Potential Columns
Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size L7 Potential Columns
A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size L8 Potential Columns
Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size L9 Potential Columns
Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size L10 Potential Columns
Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size L11 Potential Columns
Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size L13 Potential Columns
Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger, 5 to 10 μm particle size L14 Potential Columns
Methylsilane groups chemically bound to completely porous silica gel, 3 to 10 μm particle size L15 Potential Columns
Dimethylsilane chemically bound to porous silica gel, 3 to 10 μm particle size L16 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form, 7 to 11 μm particle size L17 Potential Columns
Amino and cyano groups chemically bound to porous silica gel, 3 to 10 μm particle size L18 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form, 9μm particle size L19 Potential Columns
Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size L20 Potential Columns
Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size L21 Potential Columns
A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size L22 Potential Columns
Ion exchange resin of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, approximately 10 μm particle size L23 Potential Columns
Pack with the ability to separate compounds (in a molecular weight range of 100 to 5000 daltons (determined with polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Polymethacrylic resin cross-linked with polyhydroxilated ether (surface containing residual carboxyl group content) was found to be appropriate L25 Potential Columns
Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size L26 Potential Columns
Chiral ligand exchange material with L-proline copper complex covalently bound to broken silica gel, 5 to 10 μm particle size L32 Potential Columns
Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form, 9μm particle size L34 Potential Columns
Polymethacrylate gel pack with the ability to separate proteins in a molecular weight range between 2,000 and 40,000 daltons by molecular size L37 Potential Columns
Size exclusion Pack for water soluble paints based on methacrylate L38 Potential Columns
Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin L39 Potential Columns
Cellulose tris-3,5-dimethylphenylcarbamate on porous silica gel, 5 to 20 μm particle size L40 Potential Columns
Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel, 5 μm particle size L41 Potential Columns
Pentafluorophenyl groups are chemically bound to silica gel, 5 to 10 μm particle size L43 Potential Columns
High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size L47 Potential Columns
Amylose tris-3,5-dimethylphenylcarbamate on porous, spherical silica gel, 5 to 10 μm particle size L51 Potential Columns
Ovomucoid (chiral recognition protein). Chemically bound to silica particles, approximately 5μm particle size, 120 angstrom pore size L57 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the sodium (Na +) form, 7 to 11 μm particle size L58 Potential Columns
Spherical, porous silica gel with a covalent surface modification with alkylamide groups with end capping, 3-5 μm particle size L60 Potential Columns
C30 silane is bound to a completely porous silica gel, 3 to 15 μm L62 Potential Columns