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Before the development of reversed phases the normal phase chromatography (NP) was the most established separation method in analytics. Therefore normal phase HPLC columns by all world-known manufacturers are offered in wide range at the analytics-shop.com. Please, use our HPLC column configurator for more HPLC Columns choices. If you have any questions regarding normal phase HPLC columns or columns manufacturers contact us for expert assistance & advice.  Here you will find all brands from one source and at 2% online discount.

The following gives a small overview of the selection in our shop:

  • Manufacturer: Agilent, Grace, Thermo Fisher, Waters, etc.
  • Packing: Alltima, Hypersil,SunFire, Zorbax, etc.
  • Pore Size: 60 Å, 80 Å, 100 Å, 120 Å, 300 Å, etc.

 

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Normal Phase (NP)

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  1. Altmann Analytik

    HPLC Column Lichrosorb 60 Si 5µm 125x4

    Regular Price: $599.51

    Special Price $569.53

    sku: AANLS6S-5012540
  2. Altmann Analytik

    HPLC Column Lichrospher 100 Si 10µm 250x4

    Regular Price: $599.51

    Special Price $569.53

    sku: AANLI1S-10025040
  3. Altmann Analytik

    HPLC Column Lichrospher 100 Si 5µm 250x4

    Regular Price: $599.51

    Special Price $569.53

    sku: AANLI1S-5025040
  4. Altmann Analytik

    HPLC Column Lichrospher 60 Si 5µm 125x4

    Regular Price: $424.65

    Special Price $403.42

    sku: AANLI6S-5012540
  5. Grace

    Apex Silica Column 250x4.6mm 5µ old no. 4M25300

    Regular Price: $649.54

    Special Price $617.06

    sku: GR5111024
  6. Altmann Analytik

    HPLC Column Lichrosorb 60 Si 5µm 100x4,6

    Regular Price: $599.51

    Special Price $569.53

    sku: AANLS6S-5010046
  7. Altmann Analytik

    HPLC Column Lichrosorb 60 Si 5µm 250x4

    Regular Price: $722.48

    Special Price $686.36

    sku: AANLS6S-5025040
  8. Grace

    Genesis Silica Column 250x4.6mm 4µ old no. FM25961E

    Regular Price: $789.70

    Special Price $750.22

    sku: GR5109784
  9. Grace

    Apollo Silica Column 250x4.6mm 5µ old no. 36513

    Regular Price: $575.69

    Special Price $546.91

    sku: GR5126769
  10. Grace

    Apollo Silica Column-W 250x4.6mm 5µ old no. 36523

    Regular Price: $575.69

    Special Price $546.91

    sku: GR5126775
  11. Grace

    Alltima Silica Rocket 53x7mm 3µ old no. 43877

    Regular Price: $705.31

    Special Price $670.04

    sku: GR5127016
  12. Grace

    Alltima Silica Column 150x10mm 5µ old no. 81117

    Regular Price: $1,540.21

    Special Price $1,463.20

    sku: GR5129224
  13. Grace

    Alltima Silica Column 150x4.6mm 3µ old no. 81409

    Regular Price: $869.57

    Special Price $826.09

    sku: GR5129316
  14. Grace

    Alltima Silica Column-W 150x4.6mm 3µ old no. 81410

    Regular Price: $869.57

    Special Price $826.09

    sku: GR5129317
  15. Grace

    AlltimaHP Silica Column 150x3mm 3µ old no. 87619

    Regular Price: $872.59

    Special Price $828.96

    sku: GR5129532
  16. Grace

    AlltimaHP Silica Column 150x3mm 5µ old no. 87620

    Regular Price: $872.59

    Special Price $828.96

    sku: GR5129533
  17. Grace

    AlltimaHP Silica Column 150x4.6mm 3µ old no. 87786

    Regular Price: $917.80

    Special Price $871.91

    sku: GR5129626
  18. Grace

    AlltimaHP Silica Column 150x2.1mm 3um

    Regular Price: $923.83

    Special Price $877.64

    sku: GR5129628
  19. Grace

    AlltimaHP Silica Column 150x4.6mm 5µ old no. 87797

    Regular Price: $926.84

    Special Price $880.50

    sku: GR5129635
  20. Grace

    AlltimaHP Silica Column 150x2.1mm 5µ old no. 87799

    Regular Price: $917.80

    Special Price $871.91

    sku: GR5129637
  21. Grace

    Alltima Silica Column 250x4.6mm 5µ old no. 88171

    Regular Price: $944.93

    Special Price $897.68

    sku: GR5129707
  22. Grace

    Alltima Silica Column 250x4.6mm 10um

    Regular Price: $794.22

    Special Price $754.51

    sku: GR5129732
  23. Grace

    Prevail Silica Column 100x2.1mm 3µ old no. 43805

    Regular Price: $776.13

    Special Price $737.32

    sku: GR5126981
  24. Grace

    Prevail Silica Column 150x2.1mm 5µ old no. 99273

    Regular Price: $928.35

    Special Price $881.93

    sku: GR5131385
  25. Grace

    Prevail Silica Column 250x4.6mm 5µ old no. 99277

    Regular Price: $984.12

    Special Price $934.91

    sku: GR5131389
  26. Grace

    Prevail Silica Column 150x3mm 3µ old no. 99341

    Regular Price: $940.41

    Special Price $893.39

    sku: GR5131438
  27. Grace

    Genesis SI Column 150x10mm 4µ old no. 8P15961

    Regular Price: $1,828.06

    Special Price $1,736.66

    sku: GR5153730
  28. Altmann Analytik

    HPLC Column Equisil Si 5µm 100x2

    Regular Price: $299.75

    Special Price $284.76

    sku: AANEQS-5010020
  29. Altmann Analytik

    HPLC Column Equisil Si 5µm 100x3

    Regular Price: $284.38

    Special Price $270.16

    sku: AANEQS-5010030
  30. Altmann Analytik

    HPLC Column Equisil Si 5µm 100x4

    Regular Price: $284.38

    Special Price $270.16

    sku: AANEQS-5010040
  31. Altmann Analytik

    HPLC Column Equisil Si 5µm 100x4,6

    Regular Price: $338.18

    Special Price $321.27

    sku: AANEQS-5010046
  32. Altmann Analytik

    HPLC Column Equisil Si 5µm 125x2

    Regular Price: $338.18

    Special Price $321.27

    sku: AANEQS-5012520
  33. Altmann Analytik

    HPLC Column Equisil Si 5µm 125x3

    Regular Price: $322.81

    Special Price $306.67

    sku: AANEQS-5012530
  34. Altmann Analytik

    HPLC Column Equisil Si 5µm 125x4

    Regular Price: $322.81

    Special Price $306.67

    sku: AANEQS-5012540
  35. Altmann Analytik

    HPLC Column Equisil Si 5µm 125x4,6

    Regular Price: $376.61

    Special Price $357.78

    sku: AANEQS-5012546
  36. Altmann Analytik

    HPLC Column Equisil Si 5µm 150x2

    Regular Price: $368.93

    Special Price $350.48

    sku: AANEQS-5015020
  37. Altmann Analytik

    HPLC Column Equisil Si 5µm 150x3

    Regular Price: $353.56

    Special Price $335.88

    sku: AANEQS-5015030
  38. Altmann Analytik

    HPLC Column Equisil Si 5µm 150x4

    Regular Price: $353.56

    Special Price $335.88

    sku: AANEQS-5015040
  39. Altmann Analytik

    HPLC Column Equisil Si 5µm 150x4,6

    Regular Price: $399.67

    Special Price $379.69

    sku: AANEQS-5015046
  40. Altmann Analytik

    HPLC Column Equisil Si 5µm 250x2

    Regular Price: $415.04

    Special Price $394.29

    sku: AANEQS-5025020
  41. Altmann Analytik

    HPLC Column Equisil Si 5µm 250x3

    Regular Price: $445.79

    Special Price $423.50

    sku: AANEQS-5025030
  42. Altmann Analytik

    HPLC Column Equisil Si 5µm 250x4

    Regular Price: $445.79

    Special Price $423.50

    sku: AANEQS-5025040
  43. Altmann Analytik

    HPLC Column Equisil Si 5µm 250x4,6

    Regular Price: $453.47

    Special Price $430.80

    sku: AANEQS-5025046
  44. Altmann Analytik

    HPLC Column Equisil Si 5µm 300x2

    Regular Price: $461.16

    Special Price $438.10

    sku: AANEQS-5030020
  45. Altmann Analytik

    HPLC Column Equisil Si 5µm 300x3

    Regular Price: $476.53

    Special Price $452.70

    sku: AANEQS-5030030
  46. Altmann Analytik

    HPLC Column Equisil Si 5µm 300x4

    Regular Price: $476.53

    Special Price $452.70

    sku: AANEQS-5030040
  47. Altmann Analytik

    HPLC Column Equisil Si 5µm 300x4,6

    Regular Price: $522.65

    Special Price $496.52

    sku: AANEQS-5030046
  48. Altmann Analytik

    HPLC Column Equisil Si 5µm 40x2

    Regular Price: $245.95

    Special Price $233.65

    sku: AANEQS-504020
  49. Altmann Analytik

    HPLC Column Equisil Si 5µm 50x2

    Regular Price: $269.01

    Special Price $255.56

    sku: AANEQS-505020
  50. Altmann Analytik

    HPLC Column Equisil Si 5µm 50x3

    Regular Price: $230.58

    Special Price $219.05

    sku: AANEQS-505030
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General Information about Normal Phase Chromatography

Normal phase chromatography uses the interaction of the analyte with polar functional groups on the surface of the stationary phase. These interactions are strongest when using nonpolar solutions as mobile phase. Furthermore normal phase chromatography is a very efficient separation method, cause of the large range of solvents that can be used to adust selectivity of the each separation. Through the high complexity this methods popularity decreased until today.
Under certain conditions long equilibrium time or problem with reproducability can occure caused by the sensitivity of this technique toward low concentrations of polar components in the mobile phase. Controling these problems provides normally better chromatograms as reversed phase chromatography caused by the lower viscosity  of common solvents.
Using normal phase chromatography means nonpolar mobile phases and polar stationary phases. The normal phases are made out of silica gels or aluminium oxide, which enable pure adsorption separation at the polar OH groups. The essential separation mechanism is based on varying adsorption of analyte molecules on the stationary phase´s surface.

Grace HPLC Columns - Alltima

AlltimaTM HP columns combine Grace´s best phase chemistries with high-purity silica. The result is one product family with the selectivity and performance needed to help overcome your most challenging separation needs. The column´s most important properties are better peak symmetry, long column life, the possibility for microbore or critical analysis and multiple selectivity options.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 3, 5  100 

Agilent HPLC Columns - Prep-Sil

Agilent Prep-Sil HPLC columns are made out of ultra pure type B silica with a specific surface of 400 m2/g. These HPLC columns are characterized by a high loading capacityand and result in symmetry peaks even for basic components. Furthermore Agilent Prep-Sil HPLC columns are stable in a pH range of 0.8 - 8.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 5, 10 100

 Agilent HPLC Columns - Zorbax

Zorbax SIL is a small diameter, completely porous silica particle that is used for high performance ad- sorption chromatography. Using a patented process, Zorbax SIL particles are produced by the agglutina- tion of colloidal silica to form spherical particles of uniform diameter and pore size. Columns are loaded to a uniform bed density using a proprietary, high- pressure, slurry-loading technique.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 5, 10 70