0item(s)

You have no items in your shopping cart.

2% online discount,
easy payment
Reduced delivery costs
from $250
More than
50k products

Normal Phase (NP) HPLC Columns

In case your sample insoluble in mixtures of water and organic solvent, Analytics Shop offers a range of normal-phase columns that are ideal for the retention and separation of lipophilic compounds. Normal-phase HPLC columns are packed with non-bonded silica or with silica gel modified with polar groups. Normal-phase chromatography is quite useful for the separation of positional isomers that are difficult to separate in reversed-phase mode. Due to a rigid surface, compared to the more flexible carbon chains of reversed phases, the analytes are influenced by well-defined steric interaction with polar groups. Because NPC uses nonaqueous solvents, it is useful for the analysis of strongly hydrophobic molecules such as fats, and other highly lipophilic compounds that are not water-soluble. Its main application fields are separation of polyaromatic hydrocarbons, sterols, vitamins, chlorophylls, ceramides, and other lipid extracts.

Please, use our HPLC column configurator for more HPLC Columns choices. If you have any questions regarding normal phase HPLC columns or columns manufacturers contact us for expert assistance & advice.

Product was successfully added to your shopping cart.

Normal Phase (NP)

Set Ascending Direction

  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  1. Altmann Analytik

    HPLC Column Reprosil Chiral-AGP, 5 µm 100 x 2mm

    Regular Price: $1,860.01

    Special Price $1,767.01

    sku: AANRSAGP-5010020
  2. Altmann Analytik

    HPLC Column Reprosil Chiral-AGP, 5 µm 100 x 4mm

    Regular Price: $1,860.01

    Special Price $1,767.01

    sku: AANRSAGP-5010040
  3. Altmann Analytik

    HPLC Column Reprosil Chiral-AGP, 5 µm 150 x 2mm

    Regular Price: $2,490.26

    Special Price $2,365.75

    sku: AANRSAGP-5015020
  4. Altmann Analytik

    HPLC Column Reprosil Chiral-AGP, 5 µm 50 x 2mm

    Regular Price: $1,429.60

    Special Price $1,358.12

    sku: AANRSAGP-505020
  5. Altmann Analytik

    HPLC Column Reprosil Chiral-AA, 8 µm, 250 x 4.0mm

    Regular Price: $1,806.21

    Special Price $1,715.90

    sku: AANRSCHA-8025040
  6. Altmann Analytik

    HPLC Column Reprosil Chiral-AA, 8 µm, 250 x 4,6mm

    Regular Price: $2,113.65

    Special Price $2,007.97

    sku: AANRSCHA-8025046
  7. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-10010020
  8. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-10010030
  9. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-10010040
  10. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-10010046
  11. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-10015020
  12. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-10015030
  13. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-10015040
  14. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-10015046
  15. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 250 x 10mm, Semi-Prep

    Regular Price: $4,534.74

    Special Price $4,308.00

    sku: AANRSCHB-100250100
  16. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-10025020
  17. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-10025030
  18. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-10025040
  19. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-10025046
  20. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-5010020
  21. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-5010030
  22. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-5010040
  23. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHB-5010046
  24. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-5015020
  25. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-5015030
  26. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-5015040
  27. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHB-5015046
  28. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 250 x 10mm, Semi-Prep

    Regular Price: $4,534.74

    Special Price $4,308.00

    sku: AANRSCHB-50250100
  29. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-5025020
  30. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-5025030
  31. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-5025040
  32. Altmann Analytik

    HPLC Column Reprosil Chiral-BM, 5 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHB-5025046
  33. Altmann Analytik

    HPLC Column Reprosil Chiral-OH, 8 µm, 250 x 4.0mm

    Regular Price: $1,806.21

    Special Price $1,715.90

    sku: AANRSCHH-8025040
  34. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-10010020
  35. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-10010030
  36. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-10010040
  37. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-10010046
  38. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHJ-10015020
  39. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHJ-10015030
  40. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHJ-10015040
  41. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 150 x 4,6mm

    Regular Price: $1,421.91

    Special Price $1,350.81

    sku: AANRSCHJ-10015046
  42. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 250 x 10mm, Semi-Prep

    Regular Price: $3,919.86

    Special Price $3,723.87

    sku: AANRSCHJ-100250100
  43. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHJ-10025020
  44. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHJ-10025030
  45. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHJ-10025040
  46. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 250 x 4,6mm

    Regular Price: $1,652.49

    Special Price $1,569.87

    sku: AANRSCHJ-10025046
  47. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-5010020
  48. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 10 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-5010030
  49. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-5010040
  50. Altmann Analytik

    HPLC Column Reprosil Chiral-JM, 5 µm, 100 x 4,6mm

    Regular Price: $1,268.19

    Special Price $1,204.78

    sku: AANRSCHJ-5010046
Set Ascending Direction

  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

General Information about Normal Phase Chromatography

Normal phase chromatography uses the interaction of the analyte with polar functional groups on the surface of the stationary phase. These interactions are strongest when using nonpolar solutions as mobile phase. Furthermore normal phase chromatography is a very efficient separation method, cause of the large range of solvents that can be used to adust selectivity of the each separation. Through the high complexity this methods popularity decreased until today.
Under certain conditions long equilibrium time or problem with reproducability can occure caused by the sensitivity of this technique toward low concentrations of polar components in the mobile phase. Controling these problems provides normally better chromatograms as reversed phase chromatography caused by the lower viscosity  of common solvents.
Using normal phase chromatography means nonpolar mobile phases and polar stationary phases. The normal phases are made out of silica gels or aluminium oxide, which enable pure adsorption separation at the polar OH groups. The essential separation mechanism is based on varying adsorption of analyte molecules on the stationary phase´s surface.

Grace HPLC Columns - Alltima

AlltimaTM HP columns combine Grace´s best phase chemistries with high-purity silica. The result is one product family with the selectivity and performance needed to help overcome your most challenging separation needs. The column´s most important properties are better peak symmetry, long column life, the possibility for microbore or critical analysis and multiple selectivity options.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 3, 5  100 

Agilent HPLC Columns - Prep-Sil

Agilent Prep-Sil HPLC columns are made out of ultra pure type B silica with a specific surface of 400 m2/g. These HPLC columns are characterized by a high loading capacityand and result in symmetry peaks even for basic components. Furthermore Agilent Prep-Sil HPLC columns are stable in a pH range of 0.8 - 8.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 5, 10 100

 Agilent HPLC Columns - Zorbax

Zorbax SIL is a small diameter, completely porous silica particle that is used for high performance ad- sorption chromatography. Using a patented process, Zorbax SIL particles are produced by the agglutina- tion of colloidal silica to form spherical particles of uniform diameter and pore size. Columns are loaded to a uniform bed density using a proprietary, high- pressure, slurry-loading technique.

Phase       
 
Particle Size
[µm] 
Pore Size
[Å] 
Silica 5, 10 70