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Reversed Phase (RP) Chromatography Columns

Reversed phase LC columns by top HPLC columns manufacturers are available at Analytics Shop. Now, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar (hydrophobic) stationary phase. Most of these protocols use as the mobile phase an aqueous blend of water with a miscible, polar organic solvent, such as acetonitrile or methanol. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica (sometimes called ODS) is the most popular type of reversed-phase HPLC packing. Reversed-phase HPLC is very widely applicable for small molecule analyses. Reversed- phase HPLC is a good choice for peptide and protein separations when short chain alkyl stationary phases are used.

Reversed phase HPLC is the first choice for:

- Neutral and non-polar compounds with a molecular weight less than 2000 Da

- Homologs and Benzologs

- Weak acids and bases

- Strong acids and bases (ion pair HPLC)

- Proteins and peptide

Narrow down your HPLC column search dimensions and quickly find an equivalent column in our HPLC Column Configurator.

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Reversed Phase (RP)

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  1. Thermo Fisher Scientific

    HPLC Column BDS-HYPERSIL-C18250x4.6mm 5µm

    $630.74
    sku: TF28105-254630
  2. Altmann Analytik

    HPLC Column Nucleosil 100Å C18 5µm 250x4.6mm

    $482.71
    sku: AANNS1C9-5025046
  3. Altmann Analytik

    HPLC Column Nucleosil 100Å C18 5µm 125x4mm

    $343.85
    sku: AANNS1C9-5012540
  4. Altmann Analytik

    HPLC Column Hypersil 120Å ODS 5µm 250x4.6mm

    $594.55
    sku: AANHSO-5025046
  5. Altmann Analytik

    HPLC Column Hypersil 120Å ODS 5µm 125x4.6mm

    $455.40
    sku: AANHSO-5012546
  6. Altmann Analytik

    HPLC Column Spherisorb 80Å ODS-2 5µm 125x4.6mm, endc.

    $409.98
    sku: AANSSO2-5012546
  7. Altmann Analytik

    HPLC Column Lichrospher 60Å RP-Select-B 5µm 125x4.6mm

    $426.08
    sku: AANLI6RS-5012546
  8. Altmann Analytik

    HPLC Column Lichrospher 60Å RP-Select-B 5µm 125x4mm

    $388.70
    sku: AANLI6RS-5012540
  9. Altmann Analytik

    HPLC Column Reprosil Pur 120Å C8 3µm 100x4.6mm

    $407.10
    sku: AANRSPU98-3010046
  10. Altmann Analytik

    HPLC Column Reprosil Pur 120Å ODS-3 5µm 150x4.6mm

    $434.70
    sku: AANRSPU93-5015046
  11. Altmann Analytik

    HPLC Column Reprosil Pur 120Å ODS-3 3µm 150x4.6mm

    $483.00
    sku: AANRSPU93-3015046
  12. Altmann Analytik

    HPLC Column Reprosil 100Å C8 4µm 125x4mm

    $407.10
    sku: AANRS9C8-4012540
  13. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 250x3 mm, endcapped

    $628.19
    sku: AANNS1C9A-5025030
  14. Altmann Analytik

    HPLC Column Nucleosil 100 C18-AB 5µm 125x3 mm, endcapped

    $515.78
    sku: AANNS1C9A-5012530
  15. Altmann Analytik

    HPLC Column Lichrospher 100Å RP18-endc. 5µm 250x4.6mm

    $545.68
    sku: AANLI1R9E-5025046
  16. Altmann Analytik

    HPLC Column Equisil Gold C18 3µm 150x3 mm, endcapped, 120A

    $524.40
    sku: AANEQGC9-3015030
  17. Thermo Fisher Scientific

    HPLC Column Hypersil ODS-2 Hypersil 125x4mm 5µm

    $555.12
    sku: TF31605-124030
  18. Thermo Fisher Scientific

    HPLC Column ODS HYPERSIL 250x4.6mm 5µm

    $579.17
    sku: TF30105-254630
  19. Thermo Fisher Scientific

    HPLC Column ODS HYPERSIL 150x4.6mm 5µm

    $555.12
    sku: TF30105-154630
  20. Thermo Fisher Scientific

    HPLC Column ODS HYPERSIL 100x4mm 3µm

    $577.16
    sku: TF30103-104030
  21. Macherey-Nagel

    HPLC Column NUCLEODUR 100-5 C18 ec 250x4,6mm

    $479.00
    sku: MN760002.46
  22. Macherey-Nagel

    HPLC Column NUCLEODUR 100-5 C18 ec 250x4mm

    $453.00
    sku: MN760002.40
  23. Macherey-Nagel

    HPLC Column, EC 125/4.6 NUCLEOSIL 100-7 C18

    $493.00
    sku: MN720951.46
  24. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEOSIL 100-3 C18

    $815.00
    sku: MN720133.46
  25. Macherey-Nagel

    HPLC Column, EC 150/4.6 NUCLEOSIL 100-5 C18

    $493.00
    sku: MN720120.46
  26. Macherey-Nagel

    HPLC Column, EC 125/4.6 NUCLEOSIL 120-3 C18

    $710.00
    sku: MN720040.46
  27. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEOSIL 100-10 C18

    $605.00
    sku: MN720023.46
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