Reversed phase LC columns by top HPLC columns manufacturers are available at Analytics Shop. Now, because it is more reproducible and has broad applicability, reversed-phase chromatography is used for approximately 75% of all HPLC methods. The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar (hydrophobic) stationary phase. Most of these protocols use as the mobile phase an aqueous blend of water with a miscible, polar organic solvent, such as acetonitrile or methanol. This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica (sometimes called ODS) is the most popular type of reversed-phase HPLC packing. Reversed-phase HPLC is very widely applicable for small molecule analyses. Reversed- phase HPLC is a good choice for peptide and protein separations when short chain alkyl stationary phases are used.
Reversed phase HPLC is the first choice for:
- Neutral and non-polar compounds with a molecular weight less than 2000 Da