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Sigma-Aldrich HPLC Columns
Supelco HPLC columns
 Supelco HPLC columns are available in different configurations:
Column Description: Ascentis, Bioshell, Discovery, Supelcosil, Supelcoguard
Packing Material: CN, C8, C18, Amide, Phenyl Hexyl
Pore Size: 90Ã…, 100Ã…, 120Ã…, 300Ã…
If you have any questions regarding Supelco HPLC columns, please do not hesitate to contact our product experts. To compare HPLC columns of various manufacturers within seconds, you may use our very own HPLC Column Configurator.
Whether conventional HPLC, UHPLC, or in the analysis of complex compounds, products of Supelco are an
essential part of modern analysis. Through continuous development and innovation, including the Fused-Core Technology,
HPLC columns from Supelco are among the best in the world. In the following the best known and most common packing
materials are described in detail.
Supelco HPLC Columns: Column Material Ascentis
Ascentis HPLC columns provide high purity, stability, and thus highly reproducible results. Modern binding processes
optimize the stability of the phase, while undesirable secondary interactions are minimized. Depending on the separation
problem you can choose from a wide range of possible phases to achieve the best separation result. Ascentis HPLC columns
are ideal for the use in the LC-MS as in all other modern applications and are scalable form analytic to preparative.
| Phase | Particel Size (μm) | Pore Size (Å) | pH-Range | Carbon Load | Endcapping |
| C18 | 3, 5, 10 | 100 | 2-8 | 25 | Yes |
| C8 | 3, 5, 10 | 100 | 2-8 | 15 | Yes |
| Phenyl | 3, 5 | 100 | 2-8 | 19 | Yes |
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Column Material Ascentis Express
Based on Fused-Core ® technology, Ascentis Express HPLC columns offer significant advantages compared to similar columns with fully-porous particles. Ascentis Express provides the speed and effectiveness of <2 micron particles, but with half the pressure. Due the reduced pressure Ascentis Express columns can be used in conventional HPLC and LC-MS-, UPLC-, and ultra-high-pressure systems. The reduced pressure also allows the use of longer columns, enabling improved resolution. Ascentis Express columns combine all these significant properties with high load capacity and excellent durability.
| Phase | Particel Size (μm) | Pore Size (Å) | pH-Range | Carbon Load | Endcapping |
| C18 | 2.7, 5 | 90 | 2-9 | 25 | Yes |
| C8 | 2.7, 5 | 90 | 2-9 | 15 | Yes |
| RIP-Amide | 2.7, 5 | 90 | 2-9 | 19 | Yes |
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Column Material Discovery
Sigma Aldrich Discovery HPLC Columns provide excellent peak sharpening, combined with improved resolution and efficiency.
Available are a wide field of phases, scalable from preparative to the analytical. Discovery HS columns are specially
designed pharmaceutical analysis and purifications.
| Phase | Particel Size (μm) | Pore Size (Å) | pH-Range | Carbon Load | Endcapping |
| C18 | 5 | 180 | 2-8 | 12 | Yes |
| C8 | 5 | 180 | 2-8 | 7,5 | Yes |
| Cyano | 5 | 180 | 2-8 | 4,5 | Yes |
| HS C18 | 3, 5, 10 | 120 | 2-8 | 20 | Yes |
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Column Material apHera
Sigma-Aldrich HPLC columns apHera have been developed to combine the advantages of silica and polystyrene columns.
apHera HPLC columns offer the same separation efficiency, but additionally allow the benefits of using buffered and
alkaline solutions. Furthermore, they allow to work in a pH range of 2-12. apHera amino-column based on covalently
bound amino groups and are ideally optimized for use in the LC-MS. They also have been specially optimized for the
separation of mono-and oligosaccharides.
| Phase | Particel Size (μm) | Pore Size (Å) | pH-Range | Carbon Load | Endcapping |
| C18 | 5, 9, 13 | 300 | 2-12 | 17 | - |
| C8 | 5 | 300 | 2-12 | 10 | - |
| C4 | 5 | 300 | 2-12 | 6 | - |
| NH2 | 5, 9, 13 | 300 | 2-13 | 0 | - |
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Column Material Kromasil
Kromasil silicon-based HPLC columns are scalable from analytical to industrial scale and offer at all sizes the same
excellent effectiveness. They are characterized by high chemical and mechanical stability, which results in long life
and high loading-capacity. Due to their excellent chemical stability, the reproducibility of the results obtained is
optimized, while minimizing unwanted effects.
| Phase | Particel Size (μm) | Pore Size (Å) | pH-Range | Carbon Load | Endcapping |
| C18 | 3.5, 5 | 100, 300 | -Â | 20 | - |
| C8 | 3.5, 5, 7, 10 | 100, 300 | -Â | 12 | - |
| C4 | 3.5, 5, 7, 10 | 100, 300 | -Â | 8 | - |
| Silica | 3.5, 5 | 60, 100 | -Â | 0 | - |
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Column Material Supelcosil:
Supelcosil silica-based HPLC columns are available in over 40 different bonded phases, in a large range of particle sizes
and column configurations and from analytical to preparative scale. Depending on the separation problem, there is always
the right phase to receive the best possible speration result.
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If there are any questions or problems with our products the appointment our competent team will help you personally and
without any obligation.