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VDSpher HPLC Columns - made in Germany

VDSpher HPLC Columns by the German manufacturer VDS optilab are based on spherical silica gels and offer a very wide range of pore sizes and particle diameters to solve all separation problems. VDSpher phases are based on two different basic silica gels: VDSpher Classic and VDSpher PUR. All VDSpher Classic & PUR silica gels are suitable for analytical, semi-preparative and preparative scale. To achieve the best possible column packing, VDSpher PUR phases are a good choice for analytical HPLC and VDSpher Classic phases are especially recommended for preparative chromatography. U-VDSpher columns are ideal for UHPLC applications. 

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VDS optilab HPLC Columns

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  1. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 100 mm

    Regular Price: $492.00

    Special Price $442.80

    sku: VDVPE18DG020100
  2. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 125 mm

    Regular Price: $492.00

    Special Price $442.80

    sku: VDVPE18DG020125
  3. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 150 mm

    Regular Price: $517.20

    Special Price $465.48

    sku: VDVPE18DG020150
  4. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 200 mm

    Regular Price: $537.60

    Special Price $483.84

    sku: VDVPE18DG020200
  5. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 250 mm

    Regular Price: $555.60

    Special Price $500.04

    sku: VDVPE18DG020250
  6. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 30 mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG020030
  7. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 33 (40) mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG020033
  8. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 50 mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG020050
  9. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 53 (60) mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG020053
  10. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 2 x 75 mm

    Regular Price: $474.00

    Special Price $426.60

    sku: VDVPE18DG020075
  11. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 100 mm

    Regular Price: $482.40

    Special Price $434.16

    sku: VDVPE18DG030100
  12. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 125 mm

    Regular Price: $482.40

    Special Price $434.16

    sku: VDVPE18DG030125
  13. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 150 mm

    Regular Price: $505.20

    Special Price $454.68

    sku: VDVPE18DG030150
  14. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 200 mm

    Regular Price: $518.40

    Special Price $466.56

    sku: VDVPE18DG030200
  15. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 250 mm

    Regular Price: $532.80

    Special Price $479.52

    sku: VDVPE18DG030250
  16. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 30 mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG030030
  17. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 300 mm

    Regular Price: $553.20

    Special Price $497.88

    sku: VDVPE18DG030300
  18. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 33 (40) mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG030033
  19. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 50 mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG030050
  20. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 53 (60) mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG030053
  21. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 3 x 75 mm

    Regular Price: $468.00

    Special Price $421.20

    sku: VDVPE18DG030075
  22. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 100 mm

    Regular Price: $482.40

    Special Price $434.16

    sku: VDVPE18DG040100
  23. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 125 mm

    Regular Price: $482.40

    Special Price $434.16

    sku: VDVPE18DG040125
  24. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 150 mm

    Regular Price: $505.20

    Special Price $454.68

    sku: VDVPE18DG040150
  25. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 200 mm

    Regular Price: $518.40

    Special Price $466.56

    sku: VDVPE18DG040200
  26. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 250 mm

    Regular Price: $532.80

    Special Price $479.52

    sku: VDVPE18DG040250
  27. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 30 mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG040030
  28. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 300 mm

    Regular Price: $553.20

    Special Price $497.88

    sku: VDVPE18DG040300
  29. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 33 (40) mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG040033
  30. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 50 mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG040050
  31. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 53 (60) mm

    Regular Price: $452.40

    Special Price $407.16

    sku: VDVPE18DG040053
  32. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4 x 75 mm

    Regular Price: $468.00

    Special Price $421.20

    sku: VDVPE18DG040075
  33. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 100 mm

    Regular Price: $492.00

    Special Price $442.80

    sku: VDVPE18DG046100
  34. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 125 mm

    Regular Price: $492.00

    Special Price $442.80

    sku: VDVPE18DG046125
  35. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 150 mm

    Regular Price: $517.20

    Special Price $465.48

    sku: VDVPE18DG046150
  36. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 200 mm

    Regular Price: $537.60

    Special Price $483.84

    sku: VDVPE18DG046200
  37. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 250 mm

    Regular Price: $555.60

    Special Price $500.04

    sku: VDVPE18DG046250
  38. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 30 mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG046030
  39. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 300 mm

    Regular Price: $577.20

    Special Price $519.48

    sku: VDVPE18DG046300
  40. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 33 (40) mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG046033
  41. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 50 mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG046050
  42. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 53 (60) mm

    Regular Price: $458.40

    Special Price $412.56

    sku: VDVPE18DG046053
  43. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 3 µm, 4.6 x 75 mm

    Regular Price: $474.00

    Special Price $426.60

    sku: VDVPE18DG046075
  44. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 100 mm

    Regular Price: $354.00

    Special Price $318.60

    sku: VDVPE18DJ020100
  45. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 125 mm

    Regular Price: $354.00

    Special Price $318.60

    sku: VDVPE18DJ020125
  46. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 150 mm

    Regular Price: $374.40

    Special Price $336.96

    sku: VDVPE18DJ020150
  47. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 200 mm

    Regular Price: $381.60

    Special Price $343.44

    sku: VDVPE18DJ020200
  48. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 250 mm

    Regular Price: $388.80

    Special Price $349.92

    sku: VDVPE18DJ020250
  49. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 30 mm

    Regular Price: $333.60

    Special Price $300.24

    sku: VDVPE18DJ020030
  50. VDS

    HPLC Column VDSpher PUR 100 C18-M-SE, 5 µm, 2 x 33 (40) mm

    Regular Price: $333.60

    Special Price $300.24

    sku: VDVPE18DJ020033
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VDSpher PUR and VDSpher Classic 

VDSpher phases by optilab are based on two different basic silica gels: VDSpher Classic & PUR, which differ mainly in their purity. The purity of the basic silica gel has a significant impact on the chromatographic separation because the metal content changes the properties of the silica gel and the surface. Hence, the interactions between the metal parts and the electron-rich analytes need to be considered. Due to the higher metal content of the VDSpher Classic 100 C18-E phase the analytes were retarded much stronger as compared to VDSpher PUR 100 C18-E.

All VDSpher Classic & PUR silica gels are suitable for analytical, semi-preparative and preparative scale. To achieve the best possible column packing, VDSpher PUR phases are recommended for analytical issues and VDSpher Classic phases for preparative purposes.

Normally, the standard phase VDSpher PUR 100 C18-E is a good starting point for many separations, e. g. for the isolation of natural substances, for the determination of caffeine in coffee or tea and even for the determination of amino acids. VDSpher PUR 100 C18-E is of medium hydrophobicity and shows a low silanophilic activity.

 

VDSpher MS Phases

Columns of smaller dimensions are usually preferred in LC/MS analysis. To profit from a high resolution, silica gel with a small particle size is recommended for the column. Generally, any VDSpher column can be used for LC/MS. Mass spectrometry is a particularly sensitive detection method and displays very low impurities, e.g. carry-overs from previous runs (“carry-over effect“). VDSpher MS phases are designed specially to avoid these effects.

 

VDSpher OptiAqua and VDSpher OptiAqua PUR 

VDSpher OptiAqua phases are ideally suited for very hydrophilic applications. A special polar endcapping makes it possible to use 100% water as a solvent while still maintaining reversed phase properties. 

On the basis of VDSpher Classic & PUR, six VDSpher OptiAqua phases with different modifications as well as pore sizes and particle diameters have been developed. With these phases, numerous applications can be performed in preparative as well as analytical HPLC. Also, analytes of different sizes can be handled optimally. Thus, different molar mass ranges can be covered, depending on the pore size of the modified silica gel. 

VDSpher OptiAqua phases can be used e.g. to separate polar substances. Typical analytes are antibiotics, biomolecules, drugs, nucleobases, organic acids, parabens, pesticides, sulfonamides, water-soluble vitamins and xanthines.

All VDSpher OptiAqua phases are pH-stable in a range of 2 to 7.5 and can be used without any problem up to a temperature of 60 °C.

 

VDSpher OptiBio and VDSpher OptiBio PUR

VDSpher OptiBio have been developed for biochemical analysis such as protein and peptide separation. Numerous separation phases with different modifications, pore sizes and particle diameters are available. With VDSpher OptiBio columns e. g. antibodies, oligo-nucleotides, peptides and proteins as well as other biomolecules can be examined.

All VDSpher OptiBio modifications are pH-stable over a large range and can be used without any problem up to a temperature of 65 °C.