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Thin-Layer Chromatography (TLC) Plates

The principle of thin-layer chromatography (TLC) has been known for over 100 years. The TLC is still a very common and popular separation technique for qualitative and quantitative analysis in the laboratory. As for all chromatographic methods, the aim is to separate a compound into its chemical components. The success of this method is based on its simplicity, also in terms of sample preparation, low cost, versatility, fast development time, high sensitivity and excellent reproducibility. The high efficiency is another advantage of thin-layer chromatography: up to 70 samples can be separated simultaneously on a TLC plate. The TLC is used in many industries and research areas, e.g. clinical analysis, food chemistry and pharmaceutical production.

At Analytics-Shop you will find a large selection of TLC plates for your TLC analysis. The TLC plates are available in the following specifications:

Pore size: 25A - 150A
Basis material: aluminium oxide, cellulose, diatomaceous earth, silica gel, Sil Gel, Silica Gel
Format: 1x3cm - 500x20cm
Modifications: CN, G, GF, GHL, GHLF, H, HF, HL, HLF, NH2, RP C18, RP-18, RP-18F, RP-2, RP-8

Further TLC accessories from all well-known manufacturers can also be found in the Analytics Shop. Do you have any questions about our products? Please contact us. We will be happy to advise you.

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TLC Plates

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  1. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 10x20cm, 50 pc/PAK

    $222.00
    sku: MN809022
  2. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 20x20cm, 25 pc/PAK

    $196.00
    sku: MN809023
  3. Macherey-Nagel

    TLC-plates Nano-SIL-20, 5x5cm, 100 pc/PAK

    $263.00
    sku: MN811011
  4. Macherey-Nagel

    TLC-Plates ALUGRAM Xtra SIL G, 20x20cm, 25 pc/PAK

    $155.00
    sku: MN818233
  5. Macherey-Nagel

    TLC-Plates ADAMANT, 0.25mm, 10x10cm, 25 pc/PAK

    $85.00
    sku: MN821050
  6. Macherey-Nagel

    TLC-plates SIL G-25, 5x20cm, 100 pc/PAK

    $253.00
    sku: MN809011
  7. Macherey-Nagel

    TLC-plates SIL G-25, 10x20cm, 50 pc/PAK

    $222.00
    sku: MN809012
  8. Macherey-Nagel

    TLC-plates SIL G-25, 20x20cm, 25 pc/PAK

    $196.00
    sku: MN809013
  9. Macherey-Nagel

    TLC-plates SIL G-25, 5x10cm, 50 pc/PAK

    $151.00
    sku: MN809017
  10. Macherey-Nagel

    TLC-plates SIL G-25, 5x10cm, 200 pc/PAK

    $329.00
    sku: MN809017.200
  11. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 10x10cm, 25 pc/PAK

    $85.00
    sku: MN809020
  12. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 5x20cm, 100 pc/PAK

    $253.00
    sku: MN809021
  13. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 5x10cm, 50 pc/PAK

    $151.00
    sku: MN809027
  14. Macherey-Nagel

    TLC-plates SIL G-25 UV254, 5x10cm, 200 pc/PAK

    $329.00
    sku: MN809027.200
  15. Macherey-Nagel

    TLC-plates SIL G-50, 20x20cm, 20 pc/PAK

    $261.00
    sku: MN809051
  16. Macherey-Nagel

    TLC-plates SIL G-50 UV254, 20x20cm, 20 pc/PAK

    $261.00
    sku: MN809053
  17. Macherey-Nagel

    TLC-plates SIL G-100, 20x20cm, 15 pc/PAK

    $271.00
    sku: MN809061
  18. Macherey-Nagel

    TLC-plates SIL G-100 UV254, 20x20cm, 15 pc/PAK

    $271.00
    sku: MN809063
  19. Macherey-Nagel

    TLC-plates SIL G-200, 20x20cm 12 pc/PAK

    $295.00
    sku: MN809073
  20. Macherey-Nagel

    TLC-plates SIL G-200 UV254, 20x20cm, 12 pc/PAK

    $295.00
    sku: MN809083
  21. Macherey-Nagel

    TLC-plates SIL G-25 UV 254+366, 5x20cm 100 pc/PAK

    $253.00
    sku: MN809121
  22. Macherey-Nagel

    TLC-plates SIL G-25 UV 254+366, 10x20cm 50 pc/PAK

    $222.00
    sku: MN809122
  23. Macherey-Nagel

    TLC-plates SIL G-25 UV 254+366, 20x20cm 25 pc/PAK

    $185.00
    sku: MN809123
  24. Macherey-Nagel

    TLC-plates Nano-SIL-20, 10x10cm, 25 pc/PAK

    $200.00
    sku: MN811012
  25. Macherey-Nagel

    TLC-plates Nano-SIL-20, 10x20cm, 50 pc/PAK

    $474.00
    sku: MN811013
  26. Macherey-Nagel

    TLC-plates Nano-SIL-20 UV254, 5x5cm, 100 pc/PAK

    $263.00
    sku: MN811021
  27. Macherey-Nagel

    TLC-plates Nano-SIL-20 UV254, 10x10cm, 25 pc/PAK

    $200.00
    sku: MN811022
  28. Macherey-Nagel

    TLC-plates Nano-SIL-20 UV254, 10x20cm, 50 pc/PAK

    $474.00
    sku: MN811023
  29. Macherey-Nagel

    TLC-plates DURASIL-25, 10x20cm, 50 pc/PAK

    $222.00
    sku: MN812003
  30. Macherey-Nagel

    TLC-plates DURASIL-25, 20x20cm, 25 pc/PAK

    $196.00
    sku: MN812004
  31. Macherey-Nagel

    TLC-plates DURASIL-25 UV254, 5x10cm, 50 pc/PAK

    $151.00
    sku: MN812005
  32. Macherey-Nagel

    TLC-plates DURASIL-25 UV254, 5x10cm, 200 pc/PAK

    $329.00
    sku: MN812005.200
  33. Macherey-Nagel

    TLC-plates DURASIL-25 UV254, 5x20cm, 100 pc/PAK

    $253.00
    sku: MN812006
  34. Macherey-Nagel

    TLC-plates DURASIL-25 UV254, 10x20cm, 50 pc/PAK

    $222.00
    sku: MN812007
  35. Macherey-Nagel

    TLC-plates DURASIL-25 UV254, 20x20cm, 25 pc/PAK

    $196.00
    sku: MN812008
  36. Macherey-Nagel

    HPTLC-Pl. Nano-DURASIL-20, 10x10cm, 25 pc/PAK

    $200.00
    sku: MN812010
  37. Macherey-Nagel

    HPTLC-Pl. Nano-DURASIL-20, 10x20cm, 50 pc/PAK

    $474.00
    sku: MN812011
  38. Macherey-Nagel

    HPTLC-Pl. Nano-DURASIL-20 UV254, 10x10cm, 25 pc/PAK

    $200.00
    sku: MN812013
  39. Macherey-Nagel

    HPTLC-Pl. Nano-DURASIL-20 UV254, 10x20cm, 50 pc/PAK

    $474.00
    sku: MN812014
  40. Macherey-Nagel

    TLC-Plates ALUGRAM Xtra SIL G, 5x20cm, 50 pc/PAK

    $86.00
    sku: MN818232
  41. Macherey-Nagel

    TLC-Plates ADAMANT UV254, 0.25mm, 5x10cm, 50 pc/PAK

    $146.03
    sku: MN821010
  42. Macherey-Nagel

    TLC-Plates ADAMANT UV254, 0.25mm,5x10cm, 200 pc/PAK

    $318.97
    sku: MN821010.200
  43. Macherey-Nagel

    TLC-Plates ADAMANT, 0.25mm, 5x10cm, 50 pc/PAK

    $151.00
    sku: MN821040
  44. Macherey-Nagel

    TLC-Plates ADAMANT, 0.25mm, 5x10cm, 200 pc/PAK

    $329.00
    sku: MN821040.200
  45. Macherey-Nagel

    TLC-Plates ADAMANT, 0.25mm, 20x20cm, 25 pc/PAK

    $196.00
    sku: MN821060
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Performing a TLC separation

Selection of the Solvent
The solvent is selected according to elution strength. With the help of a quick test you can test different solvents and find the suitable one. 

Sample Application
It is common to use a glass capillary as a dot or short line. Too wide substance zones during application lead to poor separation, as the zones become even wider during separation. After application, the solvent is allowed to evaporate, which can be accelerated by using a dryer.

Development
A common method is to use a development chamber. The eluent is poured into the bottom of the chamber.  The saturation of the chamber with the solvent vapour is necessary for a reproducible running distance. The TLC plate with the sample is then positioned in the chamber. The separation is complete when the eluent has risen to the upper end of the TLC plate. Now the TLC plate can be removed and dried accordingly.

Evaluation
The evaluation depends on the aim of the chromatographic analysis. For qualitative determinations it is easiest to run comparison substances. Quantitative evaluations are possible via corresponding calibration measurements. The area of the substance spots is used or a photometric evaluation is carried out on the layer. However, the latter requires more equipment.