TLC Plates

Thin-Layer Chromatography (TLC) Plates

The principle of thin-layer chromatography (TLC) has been known for over 100 years. TLC is still a very common and popular separation technique for qualitative and quantitative analysis in the laboratory. Like all chromatographic methods, the aim is to separate a compound into its chemical components. The success of this method is due to its simplicity, also in terms of sample preparation, low cost, versatility, fast development time, high sensitivity and excellent reproducibility. Thin-layer chromatography is also highly efficient: up to 70 samples can be separated simultaneously on one TLC plate. TLC is used in many industries and research areas, e.g. clinical analysis, food chemistry and pharmaceutical production.

In our Analytics Shop you will find a large selection of TLC plates for your TLC analysis. TLC plates are available in a variety of materials like aluminium oxide, cellulose, diatomaceous earth, silica gel, Sil Gel, Silica Gel, with different pore sizes and modifications.

TLC accessories from all well-known manufacturers can also be found in the Analytics Shop. Please contact us if you have any questions on our products.

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Product Image of TLC-plates ALOX-25 UV254, 5x20cm, 100 pc/PAK
TLC-plates ALOX-25 UV254, 5x20cm, 100 pc/PAK
Item number: MN807021
Brand: Macherey-Nagel
$269.10 $299.00
Product Image of TLC-Plates POLYGRAM IONEX-25 SB-AC, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM IONEX-25 SB-AC, 20x20cm, 25 pc/PAK
Item number: MN806023
Brand: Macherey-Nagel
$263.70 $293.00
Product Image of TLC-Plates POLYGRAM IONEX-25 SA-Na, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM IONEX-25 SA-Na, 20x20cm, 25 pc/PAK
Item number: MN806013
Brand: Macherey-Nagel
$263.70 $293.00
Product Image of POLYGRAM SIL G, 2.5x7.5cm, 200 pc/PAK
POLYGRAM SIL G, 2.5x7.5cm, 200 pc/PAK
Item number: MN805902
Brand: Macherey-Nagel
$171.90 $191.00
Product Image of POLYGRAM SIL G/UV254, 2.5x7.5cm, 200 pc/PAK
POLYGRAM SIL G/UV254, 2.5x7.5cm, 200 pc/PAK
Item number: MN805901
Brand: Macherey-Nagel
$171.90 $191.00
Product Image of POLYGRAM SIL G, 4x8cm, 50 pc/PAK
POLYGRAM SIL G, 4x8cm, 50 pc/PAK
Item number: MN805032
Brand: Macherey-Nagel
$46.26 $51.40
Product Image of POLYGRAM SIL G UV254, 40x20cm, 25 pc/PAK
POLYGRAM SIL G UV254, 40x20cm, 25 pc/PAK
Item number: MN805024
Brand: Macherey-Nagel
$300.60 $334.00
Product Image of TLC-Plates POLYGRAM SIL G UV254, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM SIL G UV254, 20x20cm, 25 pc/PAK
Item number: MN805023
Brand: Macherey-Nagel
$169.20 $188.00
Product Image of POLYGRAM SIL G UV254, 5x20cm, 25 pc/PAK
POLYGRAM SIL G UV254, 5x20cm, 25 pc/PAK
Item number: MN805022
Brand: Macherey-Nagel
$92.70 $103.00
Product Image of TLC-Plates POLYGRAM SIL G UV254, 4x8cm, 50 pc/PAK
TLC-Plates POLYGRAM SIL G UV254, 4x8cm, 50 pc/PAK
Item number: MN805021
Brand: Macherey-Nagel
$46.26 $51.40
Product Image of POLYGRAm SIL G UV254, roll, 500x20cm, 1 pc/PAK
POLYGRAm SIL G UV254, roll, 500x20cm, 1 pc/PAK
Item number: MN805017
Brand: Macherey-Nagel
$160.20 $178.00
Product Image of POLYGRAM SIL G, 0.25 mm, 40x20cm, 25 pc/PAK
POLYGRAM SIL G, 0.25 mm, 40x20cm, 25 pc/PAK
Item number: MN805014
Brand: Macherey-Nagel
$300.60 $334.00
Product Image of POLYGRAM SIL G, 20x20cm, 25 pc/PAK
POLYGRAM SIL G, 20x20cm, 25 pc/PAK
Item number: MN805013
Brand: Macherey-Nagel
$169.20 $188.00
Product Image of POLYGRAM SIL G, 5x20cm, 50 pc/PAK
POLYGRAM SIL G, 5x20cm, 50 pc/PAK
Item number: MN805012
Brand: Macherey-Nagel
$92.70 $103.00
Product Image of POLYGRAM SIL N-HR UV254, 20x20cm, 25 pc/PAK
POLYGRAM SIL N-HR UV254, 20x20cm, 25 pc/PAK
Item number: MN804023
Brand: Macherey-Nagel
$166.50 $185.00
Product Image of POLYGRAM SIL N-HR UV254, 5x20cm, 50 pc/PAK
POLYGRAM SIL N-HR UV254, 5x20cm, 50 pc/PAK
Item number: MN804022
Brand: Macherey-Nagel
$96.30 $107.00
Product Image of TLC-Plates POLYGRAM POLYAMID-6 UV254, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM POLYAMID-6 UV254, 20x20cm, 25 pc/PAK
Item number: MN803023
Brand: Macherey-Nagel
$263.70 $293.00
Product Image of TLC-Plates POLYGRAM POLYAMID-6 UV254, 0.1 mm, 5x20cm, 50 pc/PAK
TLC-Plates POLYGRAM POLYAMID-6 UV254, 0.1 mm, 5x20cm, 50 pc/PAK
Item number: MN803022
Brand: Macherey-Nagel
$158.40 $176.00
Product Image of TLC-Plates POLYGRAM POLYAMID-6, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM POLYAMID-6, 20x20cm, 25 pc/PAK
Item number: MN803013
Brand: Macherey-Nagel
$263.70 $293.00
Product Image of TLC-Plates POLYGRAM POLYAMID-6, 5x20cm, 50 pc/PAK
TLC-Plates POLYGRAM POLYAMID-6, 5x20cm, 50 pc/PAK
Item number: MN803012
Brand: Macherey-Nagel
$158.40 $176.00
Product Image of TLC-Plates POLYGRAM ALOX N UV254, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM ALOX N UV254, 20x20cm, 25 pc/PAK
Item number: MN802023
Brand: Macherey-Nagel
$176.40 $196.00
Product Image of TLC-Plates POLYGRAM ALOX N UV254, 5x20cm, 50 pc/PAK
TLC-Plates POLYGRAM ALOX N UV254, 5x20cm, 50 pc/PAK
Item number: MN802022
Brand: Macherey-Nagel
$119.70 $133.00
Product Image of TLC-Plates POLYGRAM ALOX N UV254, 4x8cm, 50 pc/PAK
TLC-Plates POLYGRAM ALOX N UV254, 4x8cm, 50 pc/PAK
Item number: MN802021
Brand: Macherey-Nagel
$50.04 $55.60
Product Image of TLC-Plates POLYGRAM CEL 400 UV254, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 400 UV254, 20x20cm, 25 pc/PAK
Item number: MN801123
Brand: Macherey-Nagel
$189.00 $210.00
Product Image of TLC-Plates POLYGRAM CEL 400, 0.1 mm, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 400, 0.1 mm, 20x20cm, 25 pc/PAK
Item number: MN801113
Brand: Macherey-Nagel
$189.00 $210.00
Product Image of TLC-Plates POLYGRAM CEL 300 PEI UV254, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 300 PEI UV254, 20x20cm, 25 pc/PAK
Item number: MN801063
Brand: Macherey-Nagel
$234.00 $260.00
Product Image of TLC-Plates POLYGRAM CEL 300 PEI, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 300 PEI, 20x20cm, 25 pc/PAK
Item number: MN801053
Brand: Macherey-Nagel
$234.00 $260.00
Product Image of TLC-Plates POLYGRAM CEL 300 UV254, 0.1 mm, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 300 UV254, 0.1 mm, 20x20cm, 25 pc/PAK
Item number: MN801023
Brand: Macherey-Nagel
$189.00 $210.00
Product Image of TLC-Plates POLYGRAM CEL 300 UV254, 0,1 mm, 5x20cm, 50 pc/PAK
TLC-Plates POLYGRAM CEL 300 UV254, 0,1 mm, 5x20cm, 50 pc/PAK
Item number: MN801022
Brand: Macherey-Nagel
$123.30 $137.00
Product Image of TLC-Plates POLYGRAM CEL 300, 0.1 mm, 20x20cm, 25 pc/PAK
TLC-Plates POLYGRAM CEL 300, 0.1 mm, 20x20cm, 25 pc/PAK
Item number: MN801013
Brand: Macherey-Nagel
$189.00 $210.00
Product Image of TLC-Plates POLYGRAM CEL 300, 0.1 mm, 4x8cm, 50 pc/PAK
TLC-Plates POLYGRAM CEL 300, 0.1 mm, 4x8cm, 50 pc/PAK
Item number: MN801011
Brand: Macherey-Nagel
$48.96 $54.40
Product Image of HPTLC silica gel 60 RP-18 F254s MS-grade 25 glass plates 20x10cm 25 pc/PAK
HPTLC silica gel 60 RP-18 F254s MS-grade 25 glass plates 20x10cm 25 pc/PAK
Item number: MC1511610001
Brand: Merck
$915.60
Product Image of TLC Silica gel 60 F254, aluminium sheets, 5x10cm, 50 pc/PAK
TLC Silica gel 60 F254, aluminium sheets, 5x10cm, 50 pc/PAK
Item number: MC1168340001
Brand: Merck
$133.20
Product Image of TLC silica gel 60 W, aluminium sheets, 20x20cm, 25 pc/PAK
TLC silica gel 60 W, aluminium sheets, 20x20cm, 25 pc/PAK
Item number: MC1164870001
Brand: Merck
$270.00
Product Image of TLC silica gel 60 W F254s , glass plates, 20x20cm, 25 pc/PAK
TLC silica gel 60 W F254s , glass plates, 20x20cm, 25 pc/PAK
Item number: MC1164850001
Brand: Merck
$374.40
Product Image of TLC silica gel 60 W F254s, aluminium sheets, 20x20cm, 25 pc/PAK
TLC silica gel 60 W F254s, aluminium sheets, 20x20cm, 25 pc/PAK
Item number: MC1164840001
Brand: Merck
$290.40
Product Image of HPTLC silica gel 60 CN F254s, glass plates, 10x10cm, 25 pc/PAK
HPTLC silica gel 60 CN F254s, glass plates, 10x10cm, 25 pc/PAK
Item number: MC1164640001
Brand: Merck
$1,081.33
Product Image of HPTLC silica gel 60 RP-18 F254s, glass plates 10x20cm, HPTLC glass plates 20x20cm, 25 pc/PAK
HPTLC silica gel 60 RP-18 F254s, glass plates 10x20cm, HPTLC glass plates 20x20cm, 25 pc/PAK
Item number: MC1162250001
Brand: Merck
$694.80
Product Image of HPTLC cellulose, aluminium sheets, 20x20cm, 25 pc/PAK
HPTLC cellulose, aluminium sheets, 20x20cm, 25 pc/PAK
Item number: MC1160920001
Brand: Merck
$565.20
Product Image of HPTLC silica gel 60 F254s, glass plates, 20x10cm, 25 pc/PAK
HPTLC silica gel 60 F254s, glass plates, 20x10cm, 25 pc/PAK
Item number: MC1156960001
Brand: Merck
$541.20
Product Image of TLC silica gel 60 RP-18 F254s, glass plates, 5x10cm, 25 pc/PAK
TLC silica gel 60 RP-18 F254s, glass plates, 5x10cm, 25 pc/PAK
Item number: MC1156850001
Brand: Merck
$328.80
Product Image of TLC silica gel 60 RP-8 F254s, glass plates, 5x10cm, 25 pc/PAK
TLC silica gel 60 RP-8 F254s, glass plates, 5x10cm, 25 pc/PAK
Item number: MC1156840001
Brand: Merck
$291.60
Product Image of TLC silica gel 60 RP-18 F254s, glass plates, 5x20cm, 50 pc/PAK
TLC silica gel 60 RP-18 F254s, glass plates, 5x20cm, 50 pc/PAK
Item number: MC1156830001
Brand: Merck
$681.60
Product Image of HPTLC silica gel 60 NH2F254s, glass plates, 10x10cm, 25 pc/PAK
HPTLC silica gel 60 NH2F254s, glass plates, 10x10cm, 25 pc/PAK
Item number: MC1156470001
Brand: Merck
$766.80
Product Image of HPTLC silica gel 60 WR F254s, glass plates, 20x10cm, 25 pc/PAK
HPTLC silica gel 60 WR F254s, glass plates, 20x10cm, 25 pc/PAK
Item number: MC1155520001
Brand: Merck
$847.20
Product Image of HPTLC silica gel 60 F254, glass plates, 20x20cm, Mindestbestellmenge 50 St.
HPTLC silica gel 60 F254, glass plates, 20x20cm, Mindestbestellmenge 50 St.
Item number: MC1155340001
Brand: Merck
$548.40
Product Image of HPTLC silica gel 60 RP-18 F254s with concentrating zone 20x2.5cm, glass plates, 20x10cm, 25 pc/PAK
HPTLC silica gel 60 RP-18 F254s with concentrating zone 20x2.5cm, glass plates, 20x10cm, 25 pc/PAK
Item number: MC1154980001
Brand: Merck
$1,224.00
Product Image of HPTLC LiChrospher® silica gel 60 F254s, glass plates, 20x10cm, 25 pc/PAK
HPTLC LiChrospher® silica gel 60 F254s, glass plates, 20x10cm, 25 pc/PAK
Item number: MC1154450001
Brand: Merck
$562.80
Product Image of TLC silica gel 60 RP-8 F254s, glass plates, 10x20cm, 50 pc/PAK
TLC silica gel 60 RP-8 F254s, glass plates, 10x20cm, 50 pc/PAK
Item number: MC1154240001
Brand: Merck
$1,328.00
Product Image of TLC silica gel 60 RP-18 F254s, glass plates, 10x20cm, 50 pc/PAK
TLC silica gel 60 RP-18 F254s, glass plates, 10x20cm, 50 pc/PAK
Item number: MC1154230001
Brand: Merck
$1,204.00

Selection of the Solvent
The solvent is selected according to elution strength. With the help of a quick test you can test different solvents and find the suitable one. 

Sample Application
It is common to use a glass capillary as a dot or short line. Too wide substance zones during application lead to poor separation, as the zones become even wider during separation. After application, the solvent is allowed to evaporate, which can be accelerated by using a dryer.

Development
A common method is to use a development chamber. The eluent is poured into the bottom of the chamber.  The saturation of the chamber with the solvent vapour is necessary for a reproducible running distance. The TLC plate with the sample is then positioned in the chamber. The separation is complete when the eluent has risen to the upper end of the TLC plate. Now the TLC plate can be removed and dried accordingly.

Evaluation
The evaluation depends on the aim of the chromatographic analysis. For qualitative determinations it is easiest to run comparison substances. Quantitative evaluations are possible via corresponding calibration measurements. The area of the substance spots is used or a photometric evaluation is carried out on the layer. However, the latter requires more equipment.

 

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