Ion exchange chromatography is used in practice primarily for the separation of amino acids as well as for technical water purification and drinking water production. In this process, the stationary phase carries ionizable groups. These may be covalently bound anions (cation exchangers) or cations (anion exchangers). The oppositely charged ions present in the mobile phase can thereby bind to the stationary phase. Due to the different strength of the affinity between the stationary and mobile phases, the individual compounds can be eluted separately from one another.
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