Immobilization Kit, EZ-Link, for Affinity Purification

EZ Link Immobilization Kit Formulation: Multi-component kit
Sufficient For:
Immobilizing 1 to 20mg of protein per column Kit

Contents:
EZ Link Spin Columns, 1ml, 5 columns
Binding Buffer, 500 ml
Reductant Solution, 5M, 0.5ml
Blocking Buffer, 60ml
Column Accessories

Highlights :
Activated Resin – Aldehyde-activated crosslinked 6% beaded agarose, immobilize molecules via primary amines (-NH2)

Coupling Capacity – 20 mg/per ml resin
Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
Stable, permanent immobilization – Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Multiple formats – A wide variety to choose from bottled resin slurries, complete purification kits and high-throughput compatible 96-well filter plates.
Cost-effective – reuse the same batch of resin at least five times