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Solid phase extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated with liquid-liquid extraction can be prevented, such as incomplete phase separations, less-than-quantitative recoveries, use of expensive, breakable specialty glassware and disposal of large quantities of organic solvents. SPE is more efficient than liquid-liquid extraction, yields quantitative extractions that are easy to perform, is rapid and can be automated. Solvent use and lab time are reduced. SPE is used most often to prepare liquid samples and extract semi-volatile or nonvolatile analytes, but can also be used with solids that are pre-extracted into solvents. SPE products are excellent for sample extraction, concentration and cleanup. They are available in a wide variety of chemistries, adsorbents, and sizes. Selecting the most suitable product for each application and sample is important.

The advantage of the SPE is in the purity of the extracts. Thus, it is especially suitable for sophisticated analysis, such as trace analysis. With the right choice of SPE, sorbent required substances can be extracted and enriched selectively, even if they have different polarities. SPE cartridges are available with many different sorbents. These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase.

The following specifications of SPE cartridges are available:

  • Volume: 0.01 ml - 200 ml
  • Particle Size: 5 µm - 250 µm
  • Pore Size: 40 Å - 450 Å

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SPE Cartridges

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  1. Waters

    SPE Cartridge, OASIS HLB 3cc, 60 mg, 100/PK

    $300.00
    sku: WTWAT094226
  2. Agilent Technologies

    SPE Cartridge Bond Elut-PH, 500 mg, 3 ml, 50 pc/PAK

    $169.00
    sku: AG12102032
  3. Agilent Technologies

    SPE Cartridge Bond Elut-SCX, 500 mg, 3 ml, 50 pc/PAK

    $237.00
    sku: AG12102040
  4. Agilent Technologies

    SPE Cartridge Bond Elut-C18 EWP, 50 mg, 1 ml, 100 pc/PAK

    $245.00
    sku: AG12102136
  5. Agilent Technologies

    SPE Cartridge Mega Bond Elut-PSA, 1 g, 6 ml, 30 pc/PAK

    $201.00
    sku: AG12256140
  6. Agilent Technologies

    SPE Cartridge HF Mega BE-SI, 10 g,m 60 ml, 16 pc/PAK

    $209.00
    sku: AG14256034
  7. Macherey-Nagel

    SPE Cartridge, Chromafix C18 (S)

    $149.00
    sku: MN731801
  8. Thermo Fisher Scientific

    SPE Cartridge, 6 ml with 500 mg PSA, 250g Carbon, Teflon Frit, 30/PAK

    $288.54
    sku: TF60105-208
  9. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Retain PEP 500 mg, 6 ml 30/PAK

    $355.32
    sku: TF60107-206
  10. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Retain PEP 150 mg, 6 ml 30/PAK

    $195.30
    sku: TF60107-211
  11. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Silica, 500 mg/3 ml, 50/PAK

    $144.90
    sku: TF60108-315
    In Stock
  12. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Phenyl, 100 mg/1 ml, 100/PAK

    $286.02
    sku: TF60108-386
  13. Thermo Fisher Scientific

    SPE Cartridge, HyperSep C8, 200 mg/3 ml, 50/PAK

    $154.98
    sku: TF60108-393
  14. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Florisil, 50 mg, /1 ml, 100/PAK

    $318.78
    sku: TF60108-402
  15. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SCX, 50 mg, /1 ml, 100/PAK

    $268.38
    sku: TF60108-420
  16. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SCX, 1g/6 ml 30/PAK

    $219.24
    sku: TF60108-433
  17. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Phenyl 5 g,/25mL 20/PAK

    $260.82
    sku: TF60108-708
  18. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Verify-CX 500 mg/6 ml, 30/PAK

    $240.66
    sku: TF60108-723
  19. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Florisil 2g/15mL 20/PAK

    $156.24
    sku: TF60108-735
  20. Thermo Fisher Scientific

    SPE Cartridge, HYPERSEP VERIFY-CX 200 mg, 3 ml, 50/PAK

    $173.88
    sku: TF60108-777
  21. Thermo Fisher Scientific

    SPE Cartridge, SOLA-AX SPE Cartridge 10mg/1 ml pack of 100 pc

    $240.66
    sku: TF60109-003
  22. Thermo Fisher Scientific

    SPE Cartridge, SOLA WCX SPE, 10mg/1 mlCartridge 100 pack

    $239.40
    sku: TF60109-004
  23. Thermo Fisher Scientific

    SPE Cartridge, SOLA WAX SPE, 10mg/1 mlCartridge 100 pack

    $239.40
    sku: TF60109-005
  24. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SLE 1g/6 ml,pH7, 30 pack

    $123.48
    sku: TF60109-1000-6-7
  25. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SLE 2g/12 ml,pH7, 20 pack

    $139.86
    sku: TF60109-2000-12-7
  26. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SLE 300 mg, 96 w/plate pH7, 1 pack

    $253.26
    sku: TF60109-300-2-7W
  27. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SLE 4g/25mL,pH9, 15 pack

    $163.80
    sku: TF60109-4000-25-9
  28. Thermo Fisher Scientific

    SPE Cartridge, HyperSep SLE 400mg, 96 w/plate pH7, 1 pack

    $253.26
    sku: TF60109-400-2-7W
  29. Thermo Fisher Scientific

    SPE Cartridge, SOLA WAX SPE, 10mg/2mL 96well plate

    $325.08
    sku: TF60309-005
  30. Thermo Fisher Scientific

    SPE Cartridge, 3.0x10mm ID Retain PEP

    $430.92
    sku: TF60310-202
  31. Thermo Fisher Scientific

    SPE Cartridge, 2.1x10mm ID Retain-CX

    $434.70
    sku: TF60310-301
  32. Thermo Fisher Scientific

    SPE Cartridge, 3.0x10mm ID Retain-CX

    $434.70
    sku: TF60310-302
  33. Thermo Fisher Scientific

    SPE Cartridge, 2.1x20mm Retain-AX

    $534.24
    sku: TF60312-401
  34. Waters

    SPE Cartridge, Oasis MCX Platte 30 mg, 1/PK

    $389.00
    sku: WT186000248
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SPE Phases

Reversed Phase:

C-18

octadecyl bonded silica

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-8

octyl bonded silica

 

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-4  

butyldimethyl bonded silica

 

Less hydrophobic than LC-8 or LC-18. For extraction of peptides and proteins.

Phenyl  

phenyl bonded silica

 

Slightly less retention than LC-18 or LC-8 material. For reversed phase extraction of nonpolar to moderately polar compounds, especially aromatic compounds.

 

Normal Phase:

CN  

cyanopropyl bonded silica

For reversed phase extraction of moderately polar compounds, normal phase extraction of polar compounds, such as aflatoxins, antibiotics, dyes, herbicides, pesticides, phenols, steroids. Weak cation exchange for carbohydrates and cationic compounds

Diol

diol bonded silica

 

For normal phase extraction of polar compounds.

NH2  

aminopropyl bonded silica

For normal phase extraction of polar compounds, weak anion exchange for carbohydrates, weak anions, and organic acids.

 

Ion Exchange:

SAX

quaternary amine bonded  silica with Cl- counterion

 
 

For strong anion exchange for anions, organic acids, nucleic acids, nucleotides, and surfactants

SCX  

sulfonic acid bonded silica  with Na+ counterion

 

For strong cation exchange for cations, antibiotics, drugs, organic bases, amino acids, catecholamines, herbicides, nucleic acid bases, nucleosides, and surfactants.

 

The following gives an overview of all SPE cartridges the analytics-shop.com offers of the most reknown producers.

Agilent SPE cartridges

                                                                                                                                                                                                   
AccuCAT Aktivkohle AL-A   AL-B AL-N Aminopropyl C1      
C18 C18 EWP C18 INT C18 OH C2 C8 CBA
Certify Certify II CH Cyano DEA Diol ENV
EnvirElut 1664 FL Focus LMS Nexus PBA PCB
PH Plexa PCK PPL PRS PSA SAX SCX
SI            

 

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