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Solid phase extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated with liquid-liquid extraction can be prevented, such as incomplete phase separations, less-than-quantitative recoveries, use of expensive, breakable specialty glassware and disposal of large quantities of organic solvents. SPE is more efficient than liquid-liquid extraction, yields quantitative extractions that are easy to perform, is rapid and can be automated. Solvent use and lab time are reduced. SPE is used most often to prepare liquid samples and extract semi-volatile or nonvolatile analytes, but can also be used with solids that are pre-extracted into solvents. SPE products are excellent for sample extraction, concentration and cleanup. They are available in a wide variety of chemistries, adsorbents, and sizes. Selecting the most suitable product for each application and sample is important.

The advantage of the SPE is in the purity of the extracts. Thus, it is especially suitable for sophisticated analysis, such as trace analysis. With the right choice of SPE, sorbent required substances can be extracted and enriched selectively, even if they have different polarities. SPE cartridges are available with many different sorbents. These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase.

The following specifications of SPE cartridges are available:

  • Volume: 0.01 ml - 200 ml
  • Particle Size: 5 µm - 250 µm
  • Pore Size: 40 Å - 450 Å

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SPE Cartridges

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  1. Waters

    SPE Cartridge, OASIS HLB 3cc, 60 mg, 100/PK

    $312.22
    sku: WTWAT094226
  2. Agilent Technologies

    Amino (NH2) - Box, 50x 3 ml tubes, 500 m

    $249.01
    sku: AG5982-1835
  3. Agilent Technologies

    Carbon - Box, 50x 3 ml tubes, 250 mg

    $361.87
    sku: AG5982-4432
  4. Macherey-Nagel

    CHROMABOND column PS-H+ 6 ml, 500 mg, PP, 30/PAK

    $222.00
    sku: MN730377
  5. Macherey-Nagel

    CHROMABOND column PS-OH- 6 ml, 500 mg, PP, 30/PAK

    $222.00
    sku: MN730378
  6. Waters

    SPE Cartridge, Bulk Porapak Rxn CX 200ml

    $526.67
    sku: WT186004569
  7. Agilent Technologies

    SPE Cartridge, Bond Elut-PH, 500 mg, 3 ml, 50 pcs.

    $147.00
    sku: AG12102032
  8. Agilent Technologies

    SPE Cartridge, Bond Elut-C18 EWP, 50 mg, 1 ml, 100 pcs.

    $204.00
    sku: AG12102136
  9. Agilent Technologies

    SPE Cartridge, Bond Elut Plexa PCX, 200 mg, 6 ml, 30 pcs.

    $189.00
    sku: AG12108206
  10. Agilent Technologies

    SPE Cartridge, Mega BE-C18, 5 g,m 20 ml, 20 pcs.

    $272.00
    sku: AG12256023
  11. Agilent Technologies

    SPE Cartridge, Mega Bond Elut-PSA, 1 g, 6 ml, 30 pcs.

    $175.00
    sku: AG12256140
  12. Agilent Technologies

    SPE Cartridge, HF Mega BE-SI, 10 g,m 60mL, 16 pcs.

    $174.00
    sku: AG14256034
  13. Macherey-Nagel

    SPE Cartridge, ChromaBond C18 ec, 1 mL, 100 mg, MPS

    $307.00
    sku: MN730011MPS
  14. Macherey-Nagel

    SPE Cartridge, ChromaBond C18 ec, 3 mL, 200 mg, MPS

    $199.00
    sku: MN730012MPS
  15. Macherey-Nagel

    SPE Cartridge, ChromaBond C18 ec, 3 mL, 500 mg, MPS

    $214.00
    sku: MN730013MPS
  16. Macherey-Nagel

    SPE Cartridge, ChromaBond C18 ec, 6 mL, 1000 mg

    $218.00
    sku: MN730015G
  17. Macherey-Nagel

    SPE Cartridge, ChromaBond NH2, 3 mL, 500 mg

    $299.00
    sku: MN730033G
  18. Macherey-Nagel

    SPE Cartridge, ChromaBond SiOH, 6 mL, 500 mg, 30

    $185.00
    sku: MN730070G
  19. Macherey-Nagel

    SPE Cartridge, ChromaBond SiOH, 3 mL, 500 mg

    $246.00
    sku: MN730073G
  20. Macherey-Nagel

    SPE Cartridge, ChromaBond SiOH, 6 mL, 1000 mg

    $193.00
    sku: MN730075G
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SPE Phases

Reversed Phase:

C-18

octadecyl bonded silica

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-8

octyl bonded silica

 

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-4  

butyldimethyl bonded silica

 

Less hydrophobic than LC-8 or LC-18. For extraction of peptides and proteins.

Phenyl  

phenyl bonded silica

 

Slightly less retention than LC-18 or LC-8 material. For reversed phase extraction of nonpolar to moderately polar compounds, especially aromatic compounds.

 

Normal Phase:

CN  

cyanopropyl bonded silica

For reversed phase extraction of moderately polar compounds, normal phase extraction of polar compounds, such as aflatoxins, antibiotics, dyes, herbicides, pesticides, phenols, steroids. Weak cation exchange for carbohydrates and cationic compounds

Diol

diol bonded silica

 

For normal phase extraction of polar compounds.

NH2  

aminopropyl bonded silica

For normal phase extraction of polar compounds, weak anion exchange for carbohydrates, weak anions, and organic acids.

 

Ion Exchange:

SAX

quaternary amine bonded  silica with Cl- counterion

 
 

For strong anion exchange for anions, organic acids, nucleic acids, nucleotides, and surfactants

SCX  

sulfonic acid bonded silica  with Na+ counterion

 

For strong cation exchange for cations, antibiotics, drugs, organic bases, amino acids, catecholamines, herbicides, nucleic acid bases, nucleosides, and surfactants.

 

The following gives an overview of all SPE cartridges the analytics-shop.com offers of the most reknown producers.

Agilent SPE cartridges

                                                                                                                                                                                                   
AccuCAT Aktivkohle AL-A   AL-B AL-N Aminopropyl C1      
C18 C18 EWP C18 INT C18 OH C2 C8 CBA
Certify Certify II CH Cyano DEA Diol ENV
EnvirElut 1664 FL Focus LMS Nexus PBA PCB
PH Plexa PCK PPL PRS PSA SAX SCX
SI            

 

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