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Solid phase extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated with liquid/liquid extraction can be prevented, such as incomplete phase separations, less-than-quantitative recoveries, use of expensive, breakable specialty glassware, and disposal of large quantities of organic solvents. SPE is more efficient than liquid/liquid extraction, yields quantitative extractions that are easy to perform, is rapid, and can be automated. Solvent use and lab time are reduced. SPE is used most often to prepare liquid samples and extract semivolatile or nonvolatile analytes, but also can be used with solids that are pre-extracted into solvents. SPE products are excellent for sample extraction, concentration, and cleanup. They are available in a wide variety of chemistries, adsorbents, and sizes. Selecting the most suitable product for each application and sample is important.

The advantage of the SPE is in the purity of the extracts. Thus it is especially suitable for sophisticated analyzes, such as e.g. trace analysis. With the right choice of SPE sorbent required substances can be extracted and enriched selectively, even if they have different polarities. SPE cartridges are available with many different sorbents. These correspond to the usual filling materials of HPLC columns, as are, for example, reversed phase, normal phase-, ion exchange- or the so-called mixed mode materials.

The following specifications of SPE cartridges are available:

  • Volume: 0.01 ml - 200 ml
  • Particle Size: 5 µm - 250 µm
  • Pore Size: 40 Å - 450 Å

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SPE Cartridges

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  1. Thermo Fisher Scientific

    SPE Cartridge, HyperSep Verify-CX 500 mg/6 ml, 30/PAK

    $204.24
    sku: TF60108-723
  2. Macherey-Nagel

    SPE Cartridge, Chromafix PS-Ag+ (S)

    $245.00
    sku: MN731866
  3. Supelco

    Supelclean EZ-POP NP, 12 ml, 20 pc/pak

    $177.50
    sku: SU54341-U
  4. Agilent Technologies

    Bond Elut LRC-Certify II, 200mg, 50 pcs.

    $225.53
    sku: AG12113051
  5. Agilent Technologies

    Bond Elut LRC-Certify, 300mg, 50 pcs.

    $216.07
    sku: AG12113052
  6. Agilent Technologies

    SPE Cartridge, Bond Elut-C18, 200 mg, 3 ml, 50 pcs.

    $216.07
    sku: AG12102025
  7. Agilent Technologies

    SPE Cartridge, Bond Elut C18, 500 mg, 3 ml, 50 pcs.

    $223.95
    sku: AG12102028
  8. Agilent Technologies

    SPE Cartridge, Bond Elut SI, 500 mg, 3 ml, 50 pcs.

    $216.07
    sku: AG12102037
  9. Agilent Technologies

    SPE Cartridge, Bond Elut PRS, 500 mg, 3 ml, 50 pcs.

    $313.85
    sku: AG12102039
  10. Agilent Technologies

    SPE Cartridge, Bond Elut-SCX, 500 mg, 3 ml, 50 pcs.

    $313.85
    sku: AG12102040
  11. Agilent Technologies

    SPE Cartridge, Bond Elut C18, 500 mg, 6 ml, 30 pcs.

    $153.86
    sku: AG12102052
  12. Agilent Technologies

    SPE Cartridge, Bond Elut Certify, 300 mg, 6 ml, 30 pcs.

    $153.86
    sku: AG12102082
  13. Agilent Technologies

    SPE Cartridge, Bond Elut-SAX, 100 mg, 3 ml, 50 pcs.

    $279.15
    sku: AG12102125
  14. Agilent Technologies

    SPE Cartridge, Bond Elut Plexa, 30 mg, 3 ml, 50 pcs.

    $196.02
    sku: AG12109303
  15. Agilent Technologies

    SPE Cartridge, Mega BE-C18, 1 g, 6 ml, 30 pcs.

    $206.61
    sku: AG12256001
  16. Agilent Technologies

    SPE Cartridge, Mega BE-SI, 5 g,m 20 ml, 20 pcs.

    $225.53
    sku: AG12256026
  17. Agilent Technologies

    SPE Cartridge, Mega BE-C18, 10 g,m 60ml, 16 pcs.

    $501.24
    sku: AG12256031
  18. Macherey-Nagel

    SPE Cartridge, Chromafix C18 ec (M)

    $170.00
    sku: MN731805
  19. Macherey-Nagel

    SPE Cartridge, Chromafix SB (M)

    $176.00
    sku: MN731835
  20. Agilent Technologies

    C18 ODS-Box, 50 x 3 ml tubes, 200 mg

    $200.30
    sku: AG5982-1132
  21. Agilent Technologies

    EVidEX - Box, 50x 3 ml tubes, 200 mg

    $241.30
    sku: AG5982-2332
  22. Agilent Technologies

    SCX Polymer - Box, 50x 3ml tubes, 60 mg

    $288.62
    sku: AG5982-3236
  23. Agilent Technologies

    Focus 10 mg 96-well SPE plate 1 pcs.

    $564.81
    sku: AGA59660
  24. Agilent Technologies

    SPE Cartridge, Mega Bond Elut PPL, 5 g,m 60ml, 16 pcs.

    $1,423.86
    sku: AG12256087
  25. Agilent Technologies

    SPE Cartridge, Dispersive SPE 2ml, High Pigment, EN

    $309.12
    sku: AG5982-5321
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SPE Phases

Reversed Phase:

C-18

octadecyl bonded silica

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-8

octyl bonded silica

 

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-4  

butyldimethyl bonded silica

 

Less hydrophobic than LC-8 or LC-18. For extraction of peptides and proteins.

Phenyl  

phenyl bonded silica

 

Slightly less retention than LC-18 or LC-8 material. For reversed phase extraction of nonpolar to moderately polar compounds, especially aromatic compounds.

 

Normal Phase:

CN  

cyanopropyl bonded silica

For reversed phase extraction of moderately polar compounds, normal phase extraction of polar compounds, such as aflatoxins, antibiotics, dyes, herbicides, pesticides, phenols, steroids. Weak cation exchange for carbohydrates and cationic compounds

Diol

diol bonded silica

 

For normal phase extraction of polar compounds.

NH2  

aminopropyl bonded silica

For normal phase extraction of polar compounds, weak anion exchange for carbohydrates, weak anions, and organic acids.

 

Ion Exchange:

SAX

quaternary amine bonded  silica with Cl- counterion

 
 

For strong anion exchange for anions, organic acids, nucleic acids, nucleotides, and surfactants

SCX  

sulfonic acid bonded silica  with Na+ counterion

 

For strong cation exchange for cations, antibiotics, drugs, organic bases, amino acids, catecholamines, herbicides, nucleic acid bases, nucleosides, and surfactants.

 

The following gives an overview of all SPE cartridges the analytics-shop.com offers of the most reknown producers.

Agilent SPE cartridges

                                                                                                                                                                                                   
AccuCAT Aktivkohle AL-A   AL-B AL-N Aminopropyl C1      
C18 C18 EWP C18 INT C18 OH C2 C8 CBA
Certify Certify II CH Cyano DEA Diol ENV
EnvirElut 1664 FL Focus LMS Nexus PBA PCB
PH Plexa PCK PPL PRS PSA SAX SCX
SI            

 

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