SPE Cartridges

Solid phase extraction (SPE) is an increasingly useful sample preparation technique. With SPE, many of the problems associated with liquid-liquid extraction can be prevented, such as incomplete phase separations, less-than-quantitative recoveries, use of expensive, breakable specialty glassware and disposal of large quantities of organic solvents. SPE is more efficient than liquid-liquid extraction, yields quantitative extractions that are easy to perform, is rapid and can be automated. Solvent use and lab time are reduced. SPE is used most often to prepare liquid samples and extract semi-volatile or nonvolatile analytes, but can also be used with solids that are pre-extracted into solvents. SPE products are excellent for sample extraction, concentration, and cleanup. They are available in a wide variety of chemistries, adsorbents, and sizes. Selecting the most suitable product for each application and sample is important.

The advantage of the SPE is in the purity of the extracts. Thus, it is especially suitable for sophisticated analysis, such as trace analysis. With the right choice of SPE, sorbent required substances can be extracted and enriched selectively, even if they have different polarities. SPE cartridges are available with many different sorbents. These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase.

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Product Image of Sep-Pak XPoSure Plus Short Cartridge, 350 mg Sorbent, 500-1000 µm, 20 pc/pk
Sep-Pak XPoSure Plus Short Cartridge, 350 mg Sorbent, 500-1000 µm, 20 pc/pk
Item number: WTWAT047205
Brand: Waters
$366.00
Product Image of SPE Cartridge, SEP-PAK VAC RC TC18 500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC RC TC18 500 mg, 50/PK
Item number: WTWAT043425
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC RC TC18 100 mg, 50/PK
SPE Cartridge, SEP-PAK VAC RC TC18 100 mg, 50/PK
Item number: WTWAT043410
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC FLORISIL 500 mg, 30/PK
SPE Cartridge, SEP-PAK VAC FLORISIL 500 mg, 30/PK
Item number: WTWAT043405
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC SILICA 6cc, 500 mg, 30/PK
SPE Cartridge, SEP-PAK VAC SILICA 6cc, 500 mg, 30/PK
Item number: WTWAT043400
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC C18 6cc, .5 g, 30/PK
SPE Cartridge, SEP-PAK VAC C18 6cc, .5 g, 30/PK
Item number: WTWAT043395
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC FLORISIL 6cc, 1G 30/PK
SPE Cartridge, SEP-PAK VAC FLORISIL 6cc, 1G 30/PK
Item number: WTWAT043390
Brand: Waters
$157.00
Product Image of SPE Cartridge, SEP-PAK VAC FLORISIL 12cc, 2G 20/PK
SPE Cartridge, SEP-PAK VAC FLORISIL 12cc, 2G 20/PK
Item number: WTWAT043385
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC Alumina N 20cc, 5 g, 20/PK
SPE Cartridge, SEP-PAK VAC Alumina N 20cc, 5 g, 20/PK
Item number: WTWAT043375
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC FLORISIL 20cc, 5 g, 20/PK
SPE Cartridge, SEP-PAK VAC FLORISIL 20cc, 5 g, 20/PK
Item number: WTWAT043370
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC SILICA 35cc, 10 g, 10/PK
SPE Cartridge, SEP-PAK VAC SILICA 35cc, 10 g, 10/PK
Item number: WTWAT043355
Brand: Waters
$188.00
Product Image of SPE Cartridge, SEP-PAK VAC C18 35cc, 10 g, 10/PK
SPE Cartridge, SEP-PAK VAC C18 35cc, 10 g, 10/PK
Item number: WTWAT043345
Brand: Waters
$251.00
Product Image of SPE Cartridge, DNPH-SILICA 20/PK
SPE Cartridge, DNPH-SILICA 20/PK
Item number: WTWAT037500
Brand: Waters
$366.00
Product Image of SPE Cartridge, SEP-PAK VAC RC SILICA 500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC RC SILICA 500 mg, 50/PK
Item number: WTWAT036950
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC RC C18 500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC RC C18 500 mg, 50/PK
Item number: WTWAT036945
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC RC C18 100 mg, 50/PK
SPE Cartridge, SEP-PAK VAC RC C18 100 mg, 50/PK
Item number: WTWAT036935
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC SILICA 20cc,/5 g, 20/PK
SPE Cartridge, SEP-PAK VAC SILICA 20cc,/5 g, 20/PK
Item number: WTWAT036930
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK VAC C18 20cc,/5 g, 20/PK
SPE Cartridge, SEP-PAK VAC C18 20cc,/5 g, 20/PK
Item number: WTWAT036925
Brand: Waters
$303.00
Product Image of SPE Cartridge, SEP-PAK VAC SILICA 12cc,/2G 20/PK
SPE Cartridge, SEP-PAK VAC SILICA 12cc,/2G 20/PK
Item number: WTWAT036920
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC C18 12cc,/2G 20/PK
SPE Cartridge, SEP-PAK VAC C18 12cc,/2G 20/PK
Item number: WTWAT036915
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC SIL 6cc,/1G 30/PK
SPE Cartridge, SEP-PAK VAC SIL 6cc,/1G 30/PK
Item number: WTWAT036910
Brand: Waters
$157.00
Product Image of SPE Cartridge, SEP-PAK VAC C18 6cc,/1G 30/PK
SPE Cartridge, SEP-PAK VAC C18 6cc,/1G 30/PK
Item number: WTWAT036905
Brand: Waters
$157.00
Product Image of SPE Cartridge, SEP-PAK TC18 Cart 1cc,/100 mg, 100/PK
SPE Cartridge, SEP-PAK TC18 Cart 1cc,/100 mg, 100/PK
Item number: WTWAT036820
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK VAC TC18 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC TC18 3cc,/500 mg, 50/PK
Item number: WTWAT036815
Brand: Waters
$178.00
Product Image of SPE Cartridge, SEP-PAK PLUS TC18 50/PK
SPE Cartridge, SEP-PAK PLUS TC18 50/PK
Item number: WTWAT036810
Brand: Waters
$240.00
Product Image of SPE Cartridge, SEP-PAK LIGHT TC18 50/PK
SPE Cartridge, SEP-PAK LIGHT TC18 50/PK
Item number: WTWAT036805
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK ENVIRONMENTAL TC18 50/PK
SPE Cartridge, SEP-PAK ENVIRONMENTAL TC18 50/PK
Item number: WTWAT036800
Brand: Waters
$314.00
Product Image of SPE Cartridge, SEP-PAK VAC TC18 6cc, 1G 30/PK
SPE Cartridge, SEP-PAK VAC TC18 6cc, 1G 30/PK
Item number: WTWAT036795
Brand: Waters
$157.00
Product Image of SPE Cartridge, SEP-PAK VAC TC18 6cc,/500 mg, 30/PK
SPE Cartridge, SEP-PAK VAC TC18 6cc,/500 mg, 30/PK
Item number: WTWAT036790
Brand: Waters
$146.00
Product Image of SPE Cartridge, SEP-PAK VAC C8 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC C8 3cc,/500 mg, 50/PK
Item number: WTWAT036780
Brand: Waters
$178.00
Product Image of SPE Cartridge, SEP-PAK PLUS C8 50/PK
SPE Cartridge, SEP-PAK PLUS C8 50/PK
Item number: WTWAT036775
Brand: Waters
$240.00
Product Image of SPE Cartridge, SEP-PAK LIGHT C8 50/PK
SPE Cartridge, SEP-PAK LIGHT C8 50/PK
Item number: WTWAT036770
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK PLUS C18 2000/PK
SPE Cartridge, SEP-PAK PLUS C18 2000/PK
Item number: WTWAT036575
Brand: Waters
$7,210.00
Product Image of SPE Cartridge, SEP-PAK PLUS ENVIRONMENTAL C18 50/PK
SPE Cartridge, SEP-PAK PLUS ENVIRONMENTAL C18 50/PK
Item number: WTWAT023635
Brand: Waters
$314.00
Product Image of SPE Cartridge, SEP-PAK QMA Cart 1cc,/100 mg, 100/PK
SPE Cartridge, SEP-PAK QMA Cart 1cc,/100 mg, 100/PK
Item number: WTWAT023620
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK NH2 Cart 1cc,/100 mg, 100/PK
SPE Cartridge, SEP-PAK NH2 Cart 1cc,/100 mg, 100/PK
Item number: WTWAT023610
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK SILICA Cart 1cc,/100 mg, 100/PK
SPE Cartridge, SEP-PAK SILICA Cart 1cc,/100 mg, 100/PK
Item number: WTWAT023595
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK C18 Cart 1cc,/100 mg, 100/PK
SPE Cartridge, SEP-PAK C18 Cart 1cc,/100 mg, 100/PK
Item number: WTWAT023590
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK LIGHT Alumina N 50/PK
SPE Cartridge, SEP-PAK LIGHT Alumina N 50/PK
Item number: WTWAT023561
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK LIGHT Alumina A 50/PK
SPE Cartridge, SEP-PAK LIGHT Alumina A 50/PK
Item number: WTWAT023549
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK LIGHT SILICA 50/PK
SPE Cartridge, SEP-PAK LIGHT SILICA 50/PK
Item number: WTWAT023537
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK LIGHT CM 50/PK
SPE Cartridge, SEP-PAK LIGHT CM 50/PK
Item number: WTWAT023531
Brand: Waters
$230.00
Product Image of SPE Cartridge, SEP-PAK LIGHT QMA 50/PK
SPE Cartridge, SEP-PAK LIGHT QMA 50/PK
Item number: WTWAT023525
Brand: Waters
$230.00
Product Image of SPE Cartridge, SEP-PAK LIGHT NH2 50/PK
SPE Cartridge, SEP-PAK LIGHT NH2 50/PK
Item number: WTWAT023513
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK LIGHT C18 50/PK
SPE Cartridge, SEP-PAK LIGHT C18 50/PK
Item number: WTWAT023501
Brand: Waters
$219.00
Product Image of SPE Cartridge, SEP-PAK VAC QMA Cart 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK VAC QMA Cart 3cc,/500 mg, 50/PK
Item number: WTWAT020850
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK DIOL Cart 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK DIOL Cart 3cc,/500 mg, 50/PK
Item number: WTWAT020845
Brand: Waters
$209.00
Product Image of SPE Cartridge, SEP-PAK NH2 Cart 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK NH2 Cart 3cc,/500 mg, 50/PK
Item number: WTWAT020840
Brand: Waters
$188.00
Product Image of SPE Cartridge, SEP-PAK CN Cart 3cc,/500 mg, 50/PK
SPE Cartridge, SEP-PAK CN Cart 3cc,/500 mg, 50/PK
Item number: WTWAT020835
Brand: Waters
$272.00
Product Image of SPE Cartridge, Sep-Pak Vac Alumina N 3cc,/500 mg, 50/PK
SPE Cartridge, Sep-Pak Vac Alumina N 3cc,/500 mg, 50/PK
Item number: WTWAT020830
Brand: Waters
$178.00

Agilent Technologies has probably the widest range of high quality SPE cartridges. For over 30 years, the Bond Elut brand name has been synonymous with quality and consistency. Agilent offers SPE cartridges for almost any matrix with over 40 bonded silica phases for highly specialised methods and polymeric phases for rapid method development. Bond Elut also has probably the most versatile formats for SPE cartridges. 

We offer SPE cartridges from J.T. Baker, who have over 25 years of experience in this market. J.T. Baker has two product lines:

Bakerbond SPE columns - if speed, high separation performance and a good cost/benefit ratio are important to you for almost any SPE analysis.
Speedisk Extraction Disks - for high capacity, throughput, precision and sample utilisation.

We offer the full range of SPE cartridges from Grace. The sorbent producer Grace stands out for its wide range of filling materials for all applications. 

Chromabond SPE cartridges from the German brand Macherey-Nagel are subject to strict quality control to ensure perfect reproducibility from batch to batch and within each batch. Careful attention to grain size distribution and pore widths allows for homogeneous flow through the column. We offer the full range of Chromabond SPE cartridges, providing an answer to almost any application challenge.

LiChrolut sorbents from Merck Millipore are subjected to the most stringent quality controls. This ensures high batch-to-batch reproducibility. Merck SPE cartridges are characterized by the validated and certified production process, which guarantees highest purity, and their high capacity.

HyperSep SPE columns from Thermo Scientific are ideal for applications in pharmaceuticals, biochemistry, environmental analysis and food analysis. The HyperSep column range is particularly broad.

Thermo Scientific SOLA SPE columns are a revolution in SPE. As the first SPE products without frits, they offer greater reproducibility and purer, more consistent extracts with lower solvent requirements and higher sensitivity. As a result, they are often superior to conventional bulk-packed SPE products.

Waters' Sep-Pak SPE cartridges have a wide range of phases for almost all applications. No other SPE cartridge is found so frequently in scientific papers. Waters certification guarantees the low presence of extractable residues. This benefits customers in environmental, pharmaceutical and food analysis.

Waters' Oasis SPE cartridges are very well suited for difficult and highly selective separations of samples. The advantages of using Oasis cartridges are high selectivity, reduced matrix effects and high sensitivity for small molecules and therapeutic peptides.

Reversed Phase

 

C-18

octadecyl bonded silica

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-8

octyl bonded silica

 

For reversed phase extraction of nonpolar to moderately polar compounds, such as antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons, parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins.

C-4  

butyldimethyl bonded silica

 

Less hydrophobic than LC-8 or LC-18. For extraction of peptides and proteins.

Phenyl  

phenyl bonded silica

 

Slightly less retention than LC-18 or LC-8 material. For reversed phase extraction of nonpolar to moderately polar compounds, especially aromatic compounds.

 

Normal Phase

 


CN  

cyanopropyl bonded silica

For reversed phase extraction of moderately polar compounds, normal phase extraction of polar compounds, such as aflatoxins, antibiotics, dyes, herbicides, pesticides, phenols, steroids. Weak cation exchange for carbohydrates and cationic compounds

Diol

diol bonded silica

 

For normal phase extraction of polar compounds.

NH2  

aminopropyl bonded silica

For normal phase extraction of polar compounds, weak anion exchange for carbohydrates, weak anions, and organic acids.

 

Ion Exchange

 

SAX

quaternary amine bonded  silica with Cl- counterion

 
 

For strong anion exchange for anions, organic acids, nucleic acids, nucleotides, and surfactants

SCX  

sulfonic acid bonded silica  with Na+ counterion

 

For strong cation exchange for cations, antibiotics, drugs, organic bases, amino acids, catecholamines, herbicides, nucleic acid bases, nucleosides, and surfactants.

 

 

Agilent

                                                                                                                                                                                                   
AccuCAT Aktivkohle AL-A   AL-B AL-N Aminopropyl C1      
C18 C18 EWP C18 INT C18 OH C2 C8 CBA
Certify Certify II CH Cyano DEA Diol ENV
EnvirElut 1664 FL Focus LMS Nexus PBA PCB
PH Plexa PCK PPL PRS PSA SAX SCX
SI            


Grace

                                                                                                                                                                                                   
Reversed Phase
Prevail C18 Standard C18 High-Flow C18 Large Pore C8    
Normal Phase
Silica Aminopropyl Cyanopropyl Diol Florisil Florisil-PR Alumina
Speciality Sorbent Specifications
Carbograph SCX SAX        

 

J.T. Baker (Bakerbond)

                                                                                                                                                                                                   
Reversed Phase
C18 C18 Light Load C18 Polar Plus C8 C2 Cyclohexyl Phenyl
Normal Phase
Cyano Amino Diol        
Absorption
Silica Gel Florisil Alumina        
Ion Exchange
Amino Quart. Amine COOH SO2OH      
Size Exclusion
Sephadex G25 Butyl HI-Propyl CBX PEI    

 

Merck Millipore (Lichrolut)

                                                                                                                                                                                                   
CN EN NH2 RP-18 RP-18e SCX TSC

 

Thermo Scientific

                                                                                                                                                                                                   
Reversed Phase (Hypersep)
Retain PEP C18 C8 Phenyl      
Normal Phase/ Reversed Phase (Hypersep)
Silica Florisil          
Normal Phase (Hypercarb)
Cyano Diol Aminopropyl        
Ion Exchange (Hypercarb)
Retain-CX Retain-AX Verify-CX Verify-AX  SCX SAX   

 

Waters (Oasis)

                                                                                                                                                                                                   
Reversed Phase
Oasis HLB Sep-Pak tC18 Sep-Pak C18 Sep-Pak C8 Sep-Pak tC2    
Reversed Phase/ Normal Phase (Sep-Pak)
Aminopropyl Cyanopropyl Diol        
Normal Phase (Sep-Pak)
Silica Florisil Alumina        
Ion Exchange & Mixed Mode
Oasis MCX Oasis WCX Accell Plus CM Oasis MAX Oasis WAX   Â