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SPME (Solid Phase Micro Extraction) is a procedure of sampling. Later the samples can be analyzed via GC or HPLC.

Sampling

Samples which are to be analyzed by gas chromatography are usually filled in so-called autosampler vials, which are sealed with a crimp lid. For sampling, an SPME needle is pierced through the septum into the sample vial. An SPME needle looks similar to a normal cannula because it is also made of stainless steel. However, this does not serve to remove or inject fluids, but helps in penetrating the septum. There is a fused silica (fused silica) or glass fiber (StableFlex) in the needle, coated on the end. This coated fiber absorbs analyte molecules from the sample. The sample can either be taken directly from the liquid phase or from the gas phase above the liquid, the so-called head-space. If the sample is to be taken out of the gas phase, a volatility of the analyte has to be given, so that enrichment from the gas phase is possible. More information about Headspace analysis can be found here.

Desorption

Following the extraction of the analyte from the liquid sample, is the desorption in order to release the analytes from the coated fiber of the SPME needle. Different methods are suitable depending on the choice of the desired analytical method. If sample is getting analyzed by of gas chromatography, the desorption can be carried out without further intermediate steps within the sample inlet of the gas chromatograph. In this case, the so called thermal desorption is used, using the high temperature within the chromatograph to dissolve the absorbed analyte molecules from the fiber and to convert them into the gas phase. The steps of thermal desorption of the analytes and transfer into the gas phase for the gas-chromatographic separation are connected and thus analyte loss is avoided or at least minimized. The temperatures of the Inlet depend on the respective temperature during the chromatographic separation, but usually are between 100 and 300 ° C.

The absorbed analyte molecules can also be dissolved from the fiber with the aid of a solvent. This method is used if later analysis is to be carried out by HPLC (high pressure liquid chromatography).

For more Information about the SPME coating materials, please click here.

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SPME - Solid Phase Micro Extraction

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  1. Agilent Technologies

    SPME fiber holder for manual sampling

    $828.00
    sku: AG391896401
  2. Agilent Technologies

    Merlin Microseal SPME kit, for Varian/Bruker 1079 GCs, 23 gauge

    $805.00
    sku: AG392609901
  3. Agilent Technologies

    Merlin microseal,SPME repl,23GA

    $254.15
    sku: AG392609902
  4. Agilent Technologies

    Liner,UI SPME P+T strght 0.75mm 5 pcs. SHM

    $85.10
    sku: AG8001-0162
  5. Agilent Technologies

    Liner,UI SPME straight wl 0.8mm 5 pcs. V-B

    $159.85
    sku: AG8004-0176
  6. Agilent Technologies

    SPME Kit 4, PDMS, PDMS/DVB and Carboxen/PDMS

    $603.75
    sku: AGSU57324U
  7. Agilent Technologies

    SPME Fiber Carboxen/DVB/PDMS Aut 80U 1cm

    $845.25
    sku: AGSU57329U
  8. Agilent Technologies

    SPME FibercarboWAX/Peg Manual 60um, 3 pcs.

    $765.90
    sku: AGSU57355U
  9. Agilent Technologies

    SPME Inlet Guide For Manual Injection

    $83.95
    sku: AGSU57356U
  10. Agilent Technologies

    SPME Fiber, PDMS, A/S, 24 ga, 100 µm, 3 pcs.

    $665.85
    sku: AG391896302
  11. Agilent Technologies

    SPME Fiber DVB/PDMS Auto Merlin 65 µm, pk/3

    $665.85
    sku: AGSU57345U
  12. Thermo Fisher Scientific

    18MM MAG SCREW CAP, SPME .045''W/ PTFE/SIL SEPTA-125/PK

    $73.74
    sku: TFC4020-48
  13. Agilent Technologies

    SPME Fiber, PDMS, manual, 24 ga, 100 D1288, 3 pcs.

    $665.85
    sku: AG391896301
  14. Agilent Technologies

    SPME Fiber, PDMS, A/S, 24 ga, 7 µm, 3 pcs.

    $665.85
    sku: AG391896303
  15. Agilent Technologies

    SPME Fiber, PDMS, manual, 24 ga, 7 µm, 3 pcs.

    $665.85
    sku: AG391896304
  16. Agilent Technologies

    SPME Fiber Polyacryl. Manual, 24 ga, 85 µm, 3 pcs.

    $665.85
    sku: AG391896305
  17. Agilent Technologies

    SPME Fiber, Polyacrylic, A/S, 24 ga, 85 µm, 3 pcs.

    $665.85
    sku: AG391896306
  18. Agilent Technologies

    SPME Kit 1: Polyacrylate, PDMS, PDMS

    $665.85
    sku: AG391896307
  19. Agilent Technologies

    Venturi kit, PEEK, for barrel nebulizer

    $665.85
    sku: AG391896308
  20. Agilent Technologies

    SPME Fiber PDMS, manual, 24 ga, 30 µm, 3 pcs.

    $665.85
    sku: AG391896309
  21. Agilent Technologies

    SPME Fiber PDMS/DVB, manual, 24 ga, 65 µm, 3 pcs.

    $665.85
    sku: AG391896313
  22. Agilent Technologies

    SPME Fiber PDMS/DVB A/S, 24 ga, 65 µm, 3 pcs.

    $665.85
    sku: AG391896314
  23. Agilent Technologies

    SPME Fiber,Carboxen-PDMS, 24 ga, 75 µm, 3 pcs.

    $665.85
    sku: AG391896315
  24. Agilent Technologies

    SPME Fiber,Carbox.PDMS, A/S, 24 ga, 75 D1037, 3 pcs.

    $665.85
    sku: AG391896316
  25. Agilent Technologies

    SPME Fiber Holder

    $526.70
    sku: AG391896402
  26. Agilent Technologies

    Su57293 SPME Fiber

    $831.45
    sku: AGCP945462
  27. Agilent Technologies

    SPME Fiber 23Ga 85 µm Polyac 3 pcs.

    $739.45
    sku: AGSU57294U
  28. Agilent Technologies

    SPME Kit 2, carboxen/PDMS, PDMS/DVB, and polyacrylate

    $698.05
    sku: AGSU57321U
  29. Agilent Technologies

    SPME Kit 3, PDMS/DVB, polyacrylate, PDMS

    $633.65
    sku: AGSU57323U
  30. Agilent Technologies

    SPME Fiber DVB/PDMS Man Stabflex 65 µm

    $629.05
    sku: AGSU57326U
  31. Agilent Technologies

    SPME Fiber, DVB/PDMS Auto Stabflex 65 µm

    $665.85
    sku: AGSU57327U
  32. Agilent Technologies

    SPME Fiber Carbox/DVB/PDMS Man 50/30 1 cm

    $665.85
    sku: AGSU57328U
  33. Agilent Technologies

    SPME Fiber Holder For Varian

    $508.30
    sku: AGSU57331
  34. Agilent Technologies

    SPME Fiber Carboxen/PDMS 85 µm Stableflex

    $635.95
    sku: AGSU57335U
  35. Agilent Technologies

    SPME Fiber, PDMS, 100 µm, A/S,23Ga, pk/3

    $660.10
    sku: AGSU57341U
  36. Agilent Technologies

    SPME Fiber, PDMS, 100 µm, Pk/3,23Ga,Man

    $665.85
    sku: AGSU57342U
  37. Agilent Technologies

    SPME Fiber Carboxen/PDMS Auto Merlin 75 µm

    $665.85
    sku: AGSU57343U
  38. Agilent Technologies

    SPME Fiber Carboxen/PDMS Man Merlin 75 µm

    $569.25
    sku: AGSU57344U
  39. Agilent Technologies

    PME Fiber DVB/PDMS Man Merlin 65 µm, pk/3

    $696.90
    sku: AGSU57346U
  40. Agilent Technologies

    SPME fiber holder for CTC autosampler

    $573.85
    sku: AGSU57347U
  41. Agilent Technologies

    SPME Fiber Carboxen/DVB/PDMS 50/30 2 cm

    $657.80
    sku: AGSU57348U
  42. Agilent Technologies

    SPME CarboWAX-Peg 23Ga for A/S, 3 pcs.

    $716.45
    sku: AGSU57354U
  43. Agilent Technologies

    SPME 15 mL stand

    $571.55
    sku: AGSU57357U
  44. Agilent Technologies

    SPME Fiber PDMS 100 µm A/S Metal Alloy

    $832.60
    sku: AGSU57928U
  45. Agilent Technologies

    SPME Fiber Assortment 23Ga, 4 pcs.

    $897.00
    sku: AGSU57284U
  46. Agilent Technologies

    SPME Fiber, 7 µm PDMS,3 pcs., 23Ga, A/S

    $752.10
    sku: AGSU57291U
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Coating materials

Through the use of different coating materials, substance groups can be selectively absorbed. The commercially available adsorbing materials which differ greatly in their polarity include: carbowax, polydivinylbenzene, polyacrylate, carboxes and PDMS (polydimethylsiloxane). Depending on the analyte, combinations of different layer thicknesses and adsorbing materials are used.

The following table lists the fibers recommended by Merck for various types of analytes (MW = molar mass):

Analyt Type Recommended Fiber
Gases and low molecular weight compounds (MW 30-225) 75 µm/85 µm Carboxen/polydimethylsiloxane
Volatiles (MW 60-275) 100 µm polydimethylsiloxane
Volatiles, amines and nitro-aromatic compounds (MW 50-300) 65 µm polydimethylsiloxane/divinylbenzene
Polar semi-volatiles (MW 80-300) 85 µm polyacrylate
Non-polar high molecular weight compounds (MW 125-600) 7 µm polydimethylsiloxane
Non-polar semi-volatiles (MW 80-500) 30 µm polydimethylsiloxane
Alcohols and polar compounds (MW 40-275) 60 µm Carbowax (PEG)
Flavor compounds: volatiles and semi-volatiles, C3-C20 (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber
Trace compound analysis (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a 2 cm StableFlex fiber
Amines and polar compounds (HPLC use only) 60 µm polydimethylsiloxane/divinylbenzene

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