0item(s)

You have no items in your shopping cart.

2% online discount,
easy payment
Reduced delivery costs
from $250
More than
50k products

SPME (Solid Phase Micro Extraction) is a procedure of sampling. Later the samples can be analyzed via GC or HPLC.

Sampling

Samples which are to be analyzed by gas chromatography are usually filled in so-called autosampler vials, which are sealed with a crimp lid. For sampling, an SPME needle is pierced through the septum into the sample vial. An SPME needle looks similar to a normal cannula because it is also made of stainless steel. However, this does not serve to remove or inject fluids, but helps in penetrating the septum. There is a fused silica (fused silica) or glass fiber (StableFlex) in the needle, coated on the end. This coated fiber absorbs analyte molecules from the sample. The sample can either be taken directly from the liquid phase or from the gas phase above the liquid, the so-called head-space. If the sample is to be taken out of the gas phase, a volatility of the analyte has to be given, so that enrichment from the gas phase is possible. More information about Headspace analysis can be found here.

Desorption

Following the extraction of the analyte from the liquid sample, is the desorption in order to release the analytes from the coated fiber of the SPME needle. Different methods are suitable depending on the choice of the desired analytical method. If sample is getting analyzed by of gas chromatography, the desorption can be carried out without further intermediate steps within the sample inlet of the gas chromatograph. In this case, the so called thermal desorption is used, using the high temperature within the chromatograph to dissolve the absorbed analyte molecules from the fiber and to convert them into the gas phase. The steps of thermal desorption of the analytes and transfer into the gas phase for the gas-chromatographic separation are connected and thus analyte loss is avoided or at least minimized. The temperatures of the Inlet depend on the respective temperature during the chromatographic separation, but usually are between 100 and 300 ° C.

The absorbed analyte molecules can also be dissolved from the fiber with the aid of a solvent. This method is used if later analysis is to be carried out by HPLC (high pressure liquid chromatography).

For more Information about the SPME coating materials, please click here.

Product was successfully added to your shopping cart.

SPME - Solid Phase Micro Extraction

Set Ascending Direction

  1. 1
  2. 2
  1. Agilent Technologies

    Vial, screw top, headspace, clear, round bottom, 10 mL, 23 x 46 mm

    Regular Price: $76.86

    Special Price $73.02

    sku: AG5188-5392
  2. Altmann Analytik

    ND20/ND18 20ml SPME vial, 75,5x22,5mm, clear, special crimp neck, 10x100/pac

    Regular Price: $376.05

    Special Price $357.25

    sku: AAV31202333
  3. SGE

    LINER VA78 0.75 MM ID SPME 5/PAK

    Regular Price: $183.18

    Special Price $174.02

    sku: SG092117
  4. Agilent Technologies

    SPME fiber holder for manual sampling

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896401
  5. Agilent Technologies

    Merlin Microseal SPME kit, for Varian/Bruker 1079 GCs, 23 gauge

    Regular Price: $807.03

    Special Price $766.68

    sku: AG392609901
  6. Agilent Technologies

    Merlin microseal,SPME repl,23GA

    Regular Price: $274.13

    Special Price $260.42

    sku: AG392609902
  7. Agilent Technologies

    Liner,UI SPME P+T strght 0.75mm 5 pcs. SHM

    Regular Price: $90.95

    Special Price $86.40

    sku: AG8001-0162
  8. Agilent Technologies

    Liner,UI SPME straight wl 0.8mm 5 pcs. V-B

    Regular Price: $172.94

    Special Price $164.29

    sku: AG8004-0176
  9. Agilent Technologies

    SPME Kit 4, PDMS, PDMS/DVB and Carboxen/PDMS

    Regular Price: $622.57

    Special Price $591.44

    sku: AGSU57324U
  10. Agilent Technologies

    SPME Fiber Carboxen/DVB/PDMS Aut 80U 1cm

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57329U
  11. Agilent Technologies

    SPME FibercarboWAX/Peg Manual 60um, 3 pcs.

    Regular Price: $777.57

    Special Price $738.69

    sku: AGSU57355U
  12. Agilent Technologies

    SPME Inlet Guide For Manual Injection

    Regular Price: $81.98

    Special Price $77.88

    sku: AGSU57356U
  13. Agilent Technologies

    SPME Fiber, PDMS, A/S, 24 ga, 100 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896302
  14. Agilent Technologies

    SPME Fiber DVB/PDMS Auto Merlin 65 µm, pk/3

    Regular Price: $719.92

    Special Price $683.92

    sku: AGSU57345U
  15. Agilent Technologies

    SPME Fiber, PDMS, manual, 24 ga, 100 D1288, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896301
  16. Agilent Technologies

    SPME Fiber, PDMS, A/S, 24 ga, 7 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896303
  17. Agilent Technologies

    SPME Fiber, PDMS, manual, 24 ga, 7 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896304
  18. Agilent Technologies

    SPME Fiber Polyacryl. Manual, 24 ga, 85 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896305
  19. Agilent Technologies

    SPME Fiber, Polyacrylic, A/S, 24 ga, 85 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896306
  20. Agilent Technologies

    SPME Kit 1: Polyacrylate, PDMS, PDMS

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896307
  21. Agilent Technologies

    Venturi kit, PEEK, for barrel nebulizer

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896308
  22. Agilent Technologies

    SPME Fiber PDMS, manual, 24 ga, 30 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896309
  23. Agilent Technologies

    SPME Fiber PDMS/DVB, manual, 24 ga, 65 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896313
  24. Agilent Technologies

    SPME Fiber PDMS/DVB A/S, 24 ga, 65 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896314
  25. Agilent Technologies

    SPME Fiber,Carboxen-PDMS, 24 ga, 75 µm, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896315
  26. Agilent Technologies

    SPME Fiber,Carbox.PDMS, A/S, 24 ga, 75 D1037, 3 pcs.

    Regular Price: $687.90

    Special Price $653.51

    sku: AG391896316
  27. Agilent Technologies

    SPME Fiber Holder

    Regular Price: $550.83

    Special Price $523.29

    sku: AG391896402
  28. Agilent Technologies

    Su57293 SPME Fiber

    Regular Price: $886.45

    Special Price $842.13

    sku: AGCP945462
  29. Agilent Technologies

    SPME Fiber 23Ga 85 µm Polyac 3 pcs.

    Regular Price: $766.04

    Special Price $727.74

    sku: AGSU57294U
  30. Agilent Technologies

    SPME Kit 2, carboxen/PDMS, PDMS/DVB, and polyacrylate

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57321U
  31. Agilent Technologies

    SPME Kit 3, PDMS/DVB, polyacrylate, PDMS

    Regular Price: $654.59

    Special Price $621.86

    sku: AGSU57323U
  32. Agilent Technologies

    SPME Kit 4, PDMS, PDMS/DVB and Carboxen/PDMS, for flavors and odors

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57325U
  33. Agilent Technologies

    SPME Fiber DVB/PDMS Man Stabflex 65 µm

    Regular Price: $649.47

    Special Price $617.00

    sku: AGSU57326U
  34. Agilent Technologies

    SPME Fiber, DVB/PDMS Auto Stabflex 65 µm

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57327U
  35. Agilent Technologies

    SPME Fiber Carbox/DVB/PDMS Man 50/30 1 cm

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57328U
  36. Agilent Technologies

    SPME Fiber Holder For Varian

    Regular Price: $525.21

    Special Price $498.95

    sku: AGSU57331
  37. Agilent Technologies

    SPME Fiber Carboxen/PDMS 85 µm Stableflex

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57335U
  38. Agilent Technologies

    SPME Fiber, PDMS, 100 µm, A/S,23Ga, pk/3

    Regular Price: $713.52

    Special Price $677.84

    sku: AGSU57341U
  39. Agilent Technologies

    SPME Fiber, PDMS, 100 µm, Pk/3,23Ga,Man

    Regular Price: $719.92

    Special Price $683.92

    sku: AGSU57342U
  40. Agilent Technologies

    SPME Fiber Carboxen/PDMS Auto Merlin 75 µm

    Regular Price: $719.92

    Special Price $683.92

    sku: AGSU57343U
  41. Agilent Technologies

    SPME Fiber Carboxen/PDMS Man Merlin 75 µm

    Regular Price: $614.88

    Special Price $584.14

    sku: AGSU57344U
  42. Agilent Technologies

    PME Fiber DVB/PDMS Man Merlin 65 µm, pk/3

    Regular Price: $719.92

    Special Price $683.92

    sku: AGSU57346U
  43. Agilent Technologies

    SPME fiber holder for CTC autosampler

    Regular Price: $593.10

    Special Price $563.45

    sku: AGSU57347U
  44. Agilent Technologies

    SPME Fiber Carboxen/DVB/PDMS 50/30 2 cm

    Regular Price: $687.90

    Special Price $653.51

    sku: AGSU57348U
  45. Agilent Technologies

    SPME CarboWAX-Peg 23Ga for A/S, 3 pcs.

    Regular Price: $741.70

    Special Price $704.62

    sku: AGSU57354U
  46. Agilent Technologies

    SPME 15 mL stand

    Regular Price: $538.02

    Special Price $511.12

    sku: AGSU57357U
  47. Agilent Technologies

    SPME Fiber PDMS 100 µm A/S Metal Alloy

    Regular Price: $816.00

    Special Price $775.20

    sku: AGSU57928U
  48. Agilent Technologies

    SPME Fiber Assortment 23Ga, 4 pcs.

    Regular Price: $928.73

    Special Price $882.29

    sku: AGSU57284U
  49. Agilent Technologies

    SPME Fiber, 7 µm PDMS,3 pcs., 23Ga, A/S

    Regular Price: $777.57

    Special Price $738.69

    sku: AGSU57291U
  50. Agilent Technologies

    SPME Fiber 23Ga DVB/Carboxen/PDMS 3 pcs.

    Regular Price: $777.57

    Special Price $738.69

    sku: AGSU57298U
Set Ascending Direction

  1. 1
  2. 2

 

Coating materials

Through the use of different coating materials, substance groups can be selectively absorbed. The commercially available adsorbing materials which differ greatly in their polarity include: carbowax, polydivinylbenzene, polyacrylate, carboxes and PDMS (polydimethylsiloxane). Depending on the analyte, combinations of different layer thicknesses and adsorbing materials are used.

The following table lists the fibers recommended by Merck for various types of analytes (MW = molar mass):

Analyt Type Recommended Fiber
Gases and low molecular weight compounds (MW 30-225) 75 µm/85 µm Carboxen/polydimethylsiloxane
Volatiles (MW 60-275) 100 µm polydimethylsiloxane
Volatiles, amines and nitro-aromatic compounds (MW 50-300) 65 µm polydimethylsiloxane/divinylbenzene
Polar semi-volatiles (MW 80-300) 85 µm polyacrylate
Non-polar high molecular weight compounds (MW 125-600) 7 µm polydimethylsiloxane
Non-polar semi-volatiles (MW 80-500) 30 µm polydimethylsiloxane
Alcohols and polar compounds (MW 40-275) 60 µm Carbowax (PEG)
Flavor compounds: volatiles and semi-volatiles, C3-C20 (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber
Trace compound analysis (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a 2 cm StableFlex fiber
Amines and polar compounds (HPLC use only) 60 µm polydimethylsiloxane/divinylbenzene

Here you can go back to the products.