Avantor HPLC Columns

Alltima HPLC Columns

The Alltima HPLC column range was developed by Alltech. Hichrom acquired the column range from Grace. Alltima phases are acid and base-deactivated, giving excellent peak shape for acids, bases, and neutrals in a single run. Polymerically bonded and double-endcapped for long column lifetimes, Alltima columns are great general purpose columns.

Key features:

• Base deactivated silica
• Stable bonding for long column lifetime
• Symmetrical peak shape

Alltima HP HPLC Columns

Alltima HP columns offer a range of different phase chemistries based on high purity silica. The AlltimaHP product family combines the selectivity and performance needed to overcome even challenging separation needs. The low column bleed makes these columns ideal for microbore applications.

Key features:

• High purity silica
• Excellent column stability
• Low to no detectable column bleed pH s
• tability from 1 to 10
• Multiple selectivity options

Apollo HPLC Columns

Originally an Alltech brand, Hichrom acquired the Apollo range of HPLC columns from Grace. Apollo HPLC columns are based on high purity, base deactivated silica for powerful separations at an economical price. They are ideal for routine analysis in educational laboratories.

Key features:

• Easy scale-up from analytical to prep
• Extended pH stability from 1.5 to 10.5

Genesis HPLC Columns

Genesis phases are based on high purity, metal-free, spherical silica. They are suitable for the analysis of a wide range of compounds.

Key features:

• Good peak shape and reproducibility
• Long column lifetime
• pH stability 1 to 10

Prevail HPLC Columns

The Prevail range exhibits long lifetimes in both highly aqueous and highly organic mobile phases. The stability of these phases is such that a single column can be switched between highly aqueous, for analysis of highly polar analytes, and highly organic, for strong retention of hydrophobic analytes.

Key features:

• Stable from highly organic to highly aqueous
• Speciality phases for specific applications
• Excellent sensitivity with microbore and ELSD applications

Vydac HPLC Columns

Vydac 300 Å columns are a commonly employed range in bioseparations. Following the acquisition of Grace HPLC ranges by Hichrom, Vydac columns continue to be available exclusively from Hichrom in capillary to microbore and analytical to preparative dimensions.

Separate biomolecules from small peptides to large intact proteins with the Vydac family of reversed-phase columns (including Vydac TP, MS, Denali, and Everest) and ion exchange (Vydac 302IC) columns. Reversed-phase columns for a polypeptide separation should be considered on the basis of the polypeptide’s hydrophobicity, with molecular weight as a secondary consideration.

Vydac TP

Vydac TP reversed-phase material consists of aliphatic groups bonded to the surface of 300 Å pore diameter silica. The large pores of the TP silica give polypeptide molecules complete access to the interior of the silica pores. Vydac TP silica is the standard that has defined large pore HPLC for polypeptide separations for nearly two decades.

Key features:

• Long column lifetime and negligible phase leaching
• Reliable protein purifications, scalable from analytical to preparative scale
• Referenced in a large number of patents and publications

Vydac MS

Vydac MS is a further development of the Vydac range for reversedphase separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact proteins.

Key features:

• 300 Å pore size spherical silica
• Four reversed-phase chemistries
• Excellent peak shape with little or no TFA
• High protein recoveries make scale-up easy

Vydac Everest

Everest columns (238EV) have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Leading 300 Å C18 chemistries have had carbon coverage in the 2.8 to 3.6 µmol m−2 range. Everest C18 coverage is in excess of 4 µmol m−2 and approximates the theoretical limit based on surface area. The increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.

Key features:

• Unique selectivity for hydrophilic and hydrophobic peptides
• 300 Å pore size spherical silica
• Excellent sensitivity with little or no TFA in mobile phase
• Ideal for complex enzymatic digests (>12 proteins)

Vydac Denali

Vydac Denali (238DE) is a 120 Å C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes. It has applications for small molecule analyses of interest to pharmaceutical and environmental laboratories.

Key features:

• High retentiveness
• LC/MS of small molecules
• Fully scalable from capillary to process