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Hamilton HPLC Columns

Hamilton specializes in the design and manufacture of precision instrumentation, liquid handling and sampling products, and also offers a wide range of premium HPLC columns. At Analytics-Shop you will find a selection of nearly 200 columns in various dimensions. Use our HPLC column configurator to find the right column for you.

For more information on Hamilton columns, please see below.

Items 1-50 of 254

Product Image of HPLC Guard Column Starter Kit, PRP-X300, 12-20 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge
HPLC Guard Column Starter Kit, PRP-X300, 12-20 µm, 4.6 x 20 mm, 1 Holder, 1 Cartridge
Item number: HT79129
Brand: Hamilton

In stock

$238.86
Product Image of HPLC Guard Column HxSil C18, 10 µm, 4.6 x 33 mm
HPLC Guard Column HxSil C18, 10 µm, 4.6 x 33 mm
Item number: HT79923
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column HxSil C8, 10 µm, 4.6 x 33 mm
HPLC Guard Column HxSil C8, 10 µm, 4.6 x 33 mm
Item number: HT79922
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column PRP-h5, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-h5, 12-20 µm, 4.6 x 33 mm
Item number: HT79921
Brand: Hamilton
$368.00
Product Image of HPLC Guard Column PRP-3, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-3, 12-20 µm, 4.6 x 33 mm
Item number: HT79920
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column PRP-X500 C+, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X500 C+, 12-20 µm, 4.6 x 33 mm
Item number: HT79919
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column PRP-C18, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-C18, 12-20 µm, 4.6 x 33 mm
Item number: HT79918
Brand: Hamilton
$368.00
Product Image of HPLC Guard Column PRP-X400 H+, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X400 H+, 12-20 µm, 4.6 x 33 mm
Item number: HT79917
Brand: Hamilton
$357.71
Product Image of HPLC Guard Column PRP-X400, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X400, 12-20 µm, 4.6 x 33 mm
Item number: HT79916
Brand: Hamilton
$357.71
Product Image of HPLC Guard Column PRP-X300, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X300, 12-20 µm, 4.6 x 33 mm
Item number: HT79915
Brand: Hamilton
$357.71
Product Image of HPLC Guard Column PRP-X200, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X200, 12-20 µm, 4.6 x 33 mm
Item number: HT79914
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column PRP-X100, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-X100, 12-20 µm, 4.6 x 33 mm
Item number: HT79913
Brand: Hamilton
$356.57
Product Image of HPLC Guard Column PRP-1, 12-20 µm, 4.6 x 33 mm
HPLC Guard Column PRP-1, 12-20 µm, 4.6 x 33 mm
Item number: HT79912
Brand: Hamilton
$356.57
Product Image of HPLC Column HxSil C18, 100Å, 3µm, 2.1 x 50 mm
HPLC Column HxSil C18, 100Å, 3µm, 2.1 x 50 mm
Item number: HT79889
Brand: Hamilton
$522.29
Product Image of HPLC Column RCX-30, 100Å, 7 µm, 4.6 x 250 mm, PEEK
HPLC Column RCX-30, 100Å, 7 µm, 4.6 x 250 mm, PEEK
Item number: HT79877
Brand: Hamilton
$884.57
Product Image of HPLC Column PRP-X100, 100Å, 7µm, 50.8 x 250mm
HPLC Column PRP-X100, 100Å, 7µm, 50.8 x 250mm
Item number: HT79876
Brand: Hamilton
$37,813.27
Product Image of HPLC Column HxSil C18, 100Å, 5µm, 4.6 x 250 mm
HPLC Column HxSil C18, 100Å, 5µm, 4.6 x 250 mm
Item number: HT79869
Brand: Hamilton
$742.86
Product Image of HPLC Column HxSil C18, 100Å, 5µm, 4.6 x 150 mm
HPLC Column HxSil C18, 100Å, 5µm, 4.6 x 150 mm
Item number: HT79868
Brand: Hamilton
$708.57
Product Image of HPLC Guard Column Starter Set, PRP-X500 C(2+), 12-20 µm, 4.6 x 20 mm, 1 Holder, 2 Cartridges
HPLC Guard Column Starter Set, PRP-X500 C(2+), 12-20 µm, 4.6 x 20 mm, 1 Holder, 2 Cartridges
Item number: HT79866
Brand: Hamilton
$261.71
Product Image of HPLC Guard Cartridge PRP-X500 C(2+), 12-20 µm, 4.6 x 20 mm, 2 pc/PAK
HPLC Guard Cartridge PRP-X500 C(2+), 12-20 µm, 4.6 x 20 mm, 2 pc/PAK
Item number: HT79865
Brand: Hamilton
$266.29
Product Image of HPLC Column PRP-X800, 100Å, 7 µm, 4.1 x 150 mm
HPLC Column PRP-X800, 100Å, 7 µm, 4.1 x 150 mm
Item number: HT79855
Brand: Hamilton
$662.86
Product Image of HPLC Column PRP-X100, 100Å, 5 µm, 2.1 x 150 mm, PEEK
HPLC Column PRP-X100, 100Å, 5 µm, 2.1 x 150 mm, PEEK
Item number: HT79852
Brand: Hamilton
$837.71
Product Image of HPLC Guard Cartridge PRP-X800 PEEK, 7 µm, 3 x 8 mm, 5 pc/PAK
HPLC Guard Cartridge PRP-X800 PEEK, 7 µm, 3 x 8 mm, 5 pc/PAK
Item number: HT79833
Brand: Hamilton
$282.29
Product Image of HPLC Guard Cartridge PRP-X800, 7 µm, 2.1 x 20 mm, 5 pc/PAK
HPLC Guard Cartridge PRP-X800, 7 µm, 2.1 x 20 mm, 5 pc/PAK
Item number: HT79832
Brand: Hamilton
$257.14
Product Image of HPLC Guard Column Starter Set, PRP-X800 PEEK, 7 µm, 3 x 8 mm, 1 Holder, 2 Cartridges
HPLC Guard Column Starter Set, PRP-X800 PEEK, 7 µm, 3 x 8 mm, 1 Holder, 2 Cartridges
Item number: HT79831
Brand: Hamilton
$322.29
Product Image of HPLC Guard Column Starter Set, PRP-X800, 7 µm, 2.1 x 20 mm, 1 Holder, 2 Cartridges
HPLC Guard Column Starter Set, PRP-X800, 7 µm, 2.1 x 20 mm, 1 Holder, 2 Cartridges
Item number: HT79830
Brand: Hamilton
$235.43
Product Image of HPLC Column PRP-X800, 100Å, 7 µm, 4.1 x 250 mm
HPLC Column PRP-X800, 100Å, 7 µm, 4.1 x 250 mm
Item number: HT79828
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 4.1 x 250 mm
HPLC Column PRP-1, 100Å, 5 µm, 4.1 x 250 mm
Item number: HT79820
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-X100, 100Å, 5 µm, 4.1 x 150 mm
HPLC Column PRP-X100, 100Å, 5 µm, 4.1 x 150 mm
Item number: HT79812
Brand: Hamilton
$788.57
Product Image of HPLC Column PRP-X100, 100Å, 5 µm, 4.1 x 50 mm
HPLC Column PRP-X100, 100Å, 5 µm, 4.1 x 50 mm
Item number: HT79810
Brand: Hamilton
$617.14
Product Image of HPLC Column RCX-30, 100Å, 7 µm, 4.1 x 250 mm
HPLC Column RCX-30, 100Å, 7 µm, 4.1 x 250 mm
Item number: HT79803
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 7 x 305 mm
HPLC Column PRP-1, 100Å, 5 µm, 7 x 305 mm
Item number: HT79795
Brand: Hamilton
$1,238.86
Product Image of HPLC Column PRP-3, 300Å, 10 µm, 4.1 x 250 mm
HPLC Column PRP-3, 300Å, 10 µm, 4.1 x 250 mm
Item number: HT79794
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 2.1 x 100 mm
HPLC Column PRP-1, 100Å, 5 µm, 2.1 x 100 mm
Item number: HT79790
Brand: Hamilton
$553.14
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 2.3 x 25 mm
HPLC Column PRP-1, 100Å, 5 µm, 2.3 x 25 mm
Item number: HT79789
Brand: Hamilton
$346.08
Product Image of HPLC Column PRP-X300, 100Å, 7 µm, 1 x 50 mm
HPLC Column PRP-X300, 100Å, 7 µm, 1 x 50 mm
Item number: HT79783
Brand: Hamilton
$1,272.39
Product Image of HPLC Column PRP-X110, 100Å, 7 µm, 1 x 100 mm
HPLC Column PRP-X110, 100Å, 7 µm, 1 x 100 mm
Item number: HT79776
Brand: Hamilton
$1,954.19
Product Image of HPLC Column PRP-X110, 100Å, 7 µm, 1 x 50 mm
HPLC Column PRP-X110, 100Å, 7 µm, 1 x 50 mm
Item number: HT79775
Brand: Hamilton
$1,272.39
Product Image of HPLC Column PRP-1, 100Å, 7 µm, 1 x 50 mm
HPLC Column PRP-1, 100Å, 7 µm, 1 x 50 mm
Item number: HT79755
Brand: Hamilton
$493.71
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 1 x 250 mm
HPLC Column PRP-1, 100Å, 5 µm, 1 x 250 mm
Item number: HT79754
Brand: Hamilton
$1,800.95
Product Image of HPLC Column PRP-1, 100Å, 5 µm, 1 x 150 mm
HPLC Column PRP-1, 100Å, 5 µm, 1 x 150 mm
Item number: HT79753
Brand: Hamilton
$906.29
Product Image of HPLC Column PRP-X110S, 100Å, 7 µm, 2.1 x 100 mm, PEEK
HPLC Column PRP-X110S, 100Å, 7 µm, 2.1 x 100 mm, PEEK
Item number: HT79743
Brand: Hamilton
$617.14
Product Image of HPLC Column PRP-X110S, 100Å, 7 µm, 4.6 x 250 mm, PEEK
HPLC Column PRP-X110S, 100Å, 7 µm, 4.6 x 250 mm, PEEK
Item number: HT79741
Brand: Hamilton
$1,018.29
Product Image of HPLC Column PRP-X110, 100Å, 7 µm, 4.6 x 150 mm, PEEK
HPLC Column PRP-X110, 100Å, 7 µm, 4.6 x 150 mm, PEEK
Item number: HT79738
Brand: Hamilton
$724.57
Product Image of HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 250 mm
HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 250 mm
Item number: HT79735
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-X110, 100Å, 7 µm, 4.1 x 250 mm
HPLC Column PRP-X110, 100Å, 7 µm, 4.1 x 250 mm
Item number: HT79734
Brand: Hamilton
$805.71
Product Image of HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 150 mm
HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 150 mm
Item number: HT79733
Brand: Hamilton
$662.86
Product Image of HPLC Column PRP-X110, 100Å, 7 µm, 4.1 x 150 mm
HPLC Column PRP-X110, 100Å, 7 µm, 4.1 x 150 mm
Item number: HT79732
Brand: Hamilton
$662.86
Product Image of HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 100 mm
HPLC Column PRP-X110S, 100Å, 7 µm, 4.1 x 100 mm
Item number: HT79731
Brand: Hamilton
$617.14
Product Image of HPLC Guard Cartridge PRP-X110 PEEK, 12-20 µm, 3 x 8 mm, 5 pc/PAK
HPLC Guard Cartridge PRP-X110 PEEK, 12-20 µm, 3 x 8 mm, 5 pc/PAK
Item number: HT79729
Brand: Hamilton
$282.29

Items 1-50 of 254

PRP

Hamilton Polymers - Poly(styrene divinylbenzene) or PS-DVB is a robust polymer material used as the base framework for all the Hamilton polymer HPLC packing materials. The high degree of cross-linking with divinylbenzene makes the polymer bead extremely durable for high pressure applications. The most important properties are:

  • Can handle pressures over 5000psi
  • Have a usable pH range of 1-13 (allows the separation of biological samples in their natural state)
  • Polymer columns can be cleaned with 0.1 M sodium hydroxide to remove strongly retained material.
  • Swelling in organic solvents such as THF or chloroform is negligible because of the cross linking
  • Can be operated at much higher temperatures as compared to silica based materials
  • Excellent sample recoveries due to the lack of acidic silanol groups
  • PS‐DVB resins are similar in retention characteristics to a C18 but do have a slightly different selectivity in some cases.

Phase Particle Size in µm
PRP-1 3, 5, 7, 10
PRP-h1 5
PRP-3 3, 10
PRP-h5 5

Anion Exchange Columns

Six polymeric column packings for the separation of inorganic and organic anions, DNA and carbohydrates are offered from Hamilton. The most important properties of each column are:

  • X100: Polymeric column packing for separation of inorganic nad organic anions from 10 ppb to 500 ppm.
  • X110/110S: Polymeric anion exchange column packing for separation of inorganic and organic anions from 20 ppb to 20 ppm.
  • X500: Polymeric strong-base anion exchange packing with limited porosity for separation of proteins.
  • X600: Polymeric weak-base anion exchange packing for gradient separation of proteins and DNA oligomers.
  • RCX-10: Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates.
  • RCX-30: Polymeric anion exchange packing for isocratic or gradient separation of complex carbohydrates.

Phase Particle Size in µm
PRP-X100 3 | 10
PRP-X600 7
PRP-X110 7
PRP-X500 7
PRP-X110S 7

Cation Exchange Columns

A few polymeric column packings for separation of inorganic and organic cations, glyphosate, organic acids, and sugars are offered from Hamilton. The most important properties are:

  • X200: Analyze inorganic and organic cations using conductivity or UV detection. Separate mono or divalent cations depending on mobile phase conditions from 20 ppb to 200 ppm.
  • X400: Analyze glyphosate and its metabolite in drinking water. The PRP-X400 provides unique hydrophilic interaction separations.
  • X800: Separate transition metals or mono and divalent cations in the same run.
  • HC75: Calcium, hydrogen and lead form columns for the separation of mono and disaccharides, organic acids, sugars, and sugar alcohols.

Phase Particle Size in µm
PRP-X200 10
PRP-X800 7
PRP-X400 7
HC-75 9

Ion Exclusion Columns

Hamilton also offers one HPLC column for ion exclusion chromatography, PRP-X300. This HPLC column is a polymeric, ion exclusion column packing for the separation of alcohols and organic acids 

Phase Particle Size in µm
PRP-X300 7

HX-Sil

Hamilton HxSil C8 and C18 columns exhibit greater retention than most columns. This allows the separation of compounds that are not sufficiently retained on other C8 or C18 columns.

  • C8: Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C18.
  • C18: Hamilton HxSil C18 columns are well end-capped for retention time reproducibility and durability. HxSil C18 columns exhibit greater retention than most columns and enhances the separation of compounds that are not sufficiently retained on other C18 columns.

Phase Particle Size in µm
C18 3 | 5
C8 5