Whatman HPLC Columns

Whatman HPLC Coumns

Whatman offers a wide range of different HPLC column materials for every application. Thereby have to be differentiated between Normal Phase Media, Ion Exchange Media and Reverse Phase Media. The respective products for each application choosen from Partisil and PartiSphere product lines as well as the corresponding properties con be found in the following.

Normal Phase Media

Partisil Silica (5 and 10 µm)
This material is made out of high purity nad is used as stationary phase for adsorption chromatography. Partisil 10 is mainly employed for routine separations with high flow through rates and low pressure. On the contrary Partisil 5 is applicable in high resolution and high speed analyses. Partisil Silica is the basic material for every Partisil HPLC columns endueded with functional groups.

PartiSphere Spherical Silica (5 µm)
PartiSphere is charaterized by homogenous particles and narrow particle size distribution. The results of this are sharp separation peaks and excellent reproducability. This material is the basic for every PartiSphere packing material.

Ion Exchange Media

Partisil SAX (5 und 10 µm)
This packing material is a strong anion exchanger based on quaternary ammonium groups. Delivered as H2PO4 in methanol Partisil 10 SAX is often quoted in academic papers and international known for high separation performance of nucleotides. Combined with a solvecon as mobile phase Partisil SAX is stable in a pH range of 1.5 to 7.5. Furthermore this HPLC columns can be used for separating nucleic acids, organic acids and anorganic anions.

Partisil SCX (5 und 10 µm)
This HPLC column packing material is based on aromatic benzene sulfonic acid groups. Partisil SCX is especially suitable for separating nucleotides, amino acids, poly amines, drugs and other cationic groups. If the column is loaded with complex binders it can also be used for ligand exchange chromatography. Partisil SCX is stable at a pH range of 1.5 to 7.5.
 

PartiSphere SAX und SCX (5 µm)
PartiSphere SAX is a strong ion exchanger based on homgenous spherical silica particles with a tight particle size distribution. Furthermore this HPLC column material delivers very sharp peaks.

Reverse Phase Media

Partisil ODS (10 µm)
Partisil ODS is a C18 phase HPLC column with a carbon load of 5 % suitable for normal adsorption as well as for reversed phase chromatography.

Partisil ODS-2 (10 µm)
The high carbon load of 16 % of this polymeric phase constitutes it to one of the most nonpolar reversed phase HPLC columns with the highest retention capicity. caused by this property Partisil ODS-2 is an alternative for end capped C18 columns. The high loading capicity as well as the 10 µm large particles are suited for preparative work.
 

Partisil ODS-3 (5 und 10 µm)
Partisil ODS-3 is a C18 HPLC column made out of a polymere with a carbon load of 10.5 %. Partisil ODS-3 is the means of choice, if velocity, efficience and resolution are needed for C18 based applications. Furthermore this packing material of Whatman is end capped to reduce interferences caused by not deactivated silanole groups and to avoid the need of clocking ions. Partisil ODS-3 is used in many applications, in which optimal selectivity is important. Thereby pharmaceutical as well as natural products, together with food, biological substances and contamination determination in environment and industry are considerable application areas.
 

PartiSphere C-18 (5 µm)
PartiSphere C18 is a silica based HPLC column with homogenous spherical particles for high separation performance.

PartiSphere RTF-C18 (5 µm)
PartiSphere RTF (reduced tailing factor) is a base-deactivated C18 HPLC column. By producing this HPLC columns a company own process for an effective deactivation of free silanole groups is applicable. Additionally this packing material is extremly reliable and can be used at a pH range of 2 to 8 without any losses of performance. PartiSphere RTF-C18 is suitable for separating basic components, which have to be handled without using amino-modified mobile phases.

Partisil C8 (5 und 10 µm)
This is a momomeric, end capped C8 phase with a carbon load of 8.5 %. Partisil C8 is especially charaterized by high efficience, fast mass trensfer, as well as excellent peak sharpness and high stability toward various water based media and mixtures. This product is recommended for ion pairing chromatography.

PartiSphere C-8 (5 µm)
PartiSphere C-8 is a octyl binding phase made out of homogenous, spericalparticles with maximum resolution.

UniPrep C-8 (5 µm)
UniPrep C8 is a hydrophibic HPLC column with octyle chains as functional groups on the surface of the silica particles. This HPLC column was specially designed for the use of water based media.

Partisil PAC (5 und 10 µm) 
This is a polar amino and cyano binding phase with secundary amino groups for excellent thermal and chemical stability. The extraordinary selectivity and the fast adjustment of the equilibrium allow to applicable a wide range of chromatography types, e, g, adsorption, reversed phase and weak anion exchange. Partisil PAC is the besst HPLC column for separating hydrocarbons.
 

PartiSphere PAC (5 µm)
PartiSphere PAC is a polar cyano and amino binding phase with homogenous, spherical particles for maximum resolution.