Solvents for HPLC

High purity solvents for HPLC become increasingly important as mobile phase caused by increasing sensitivity of HPLC systems. Therefore the analytics-shop.com offers solutions with different levels of purity from J.T. Baker and Merck:

Degree of purity HPLC grade Gradient grade LC-MS grade

Solutions

2-Propanol, Acetonitril, Methanol

Acetonitril, Methanol, Water

2-Propanol, Acetonitril, Methanol, Water

Properties

  • suitable for isocratic separations
  • designed for UV or fluorescence detection
  • low fluorescence, slight waste steam pressure
  • low acidity and alkalinity

  • high UV permeability for UV-Vis and fluorescence measurements
  • slight influence on the baselinie of UV detection
  • suitable for highly sensitive measure-ments

  • decreasing of trace minerals, organics and ions
  • very low ambient noise
  • suitable for for measurements with high sensitivity


For more information about the levels of purity please click here.

14 Items

Product Image of HPLC Flushing Solvent
HPLC Flushing Solvent
Item number: AGG1969-85026
Brand: Agilent Technologies
$168.00
Product Image of 2-Propanol for analysis EMSURE® ACS,ISO,Reag. Ph Eur, 25 L
2-Propanol for analysis EMSURE® ACS,ISO,Reag. Ph Eur, 25 L
Item number: MC1096346025
Brand: Merck
$1,177.20
Product Image of Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1153332500
Brand: Merck

In stock

$119.00
Product Image of Solvent Cabinet with 4 bottles
Solvent Cabinet with 4 bottles
Item number: AG5067-1531
Brand: Agilent Technologies
$668.00
Product Image of Solvents-Plus Checkout Mix 3 x 2mL
Solvents-Plus Checkout Mix 3 x 2mL
Item number: AGG3440-85012
Brand: Agilent Technologies
Product Image of Silylating Mixture I, according to Sweeley, 10 x 1 ml
Silylating Mixture I, according to Sweeley, 10 x 1 ml
Item number: SU85431-10X1ML
Brand: Supelco
$190.67
Product Image of Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 2 L
Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 2 L
Item number: MC1037282002
Brand: Merck
$296.00
Product Image of Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 1 L
Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 1 L
Item number: MC1037281002
Brand: Merck
$194.67
Product Image of Water with 0.1% (v/v) Acetic acid hypergrade for LC-MS LiChrosolv, 2500 ml
Water with 0.1% (v/v) Acetic acid hypergrade for LC-MS LiChrosolv, 2500 ml
Item number: MC1590072500
Brand: Merck
$108.67
Product Image of Water for chromatography LiChrosolv, 4 L
Water for chromatography LiChrosolv, 4 L
Item number: MC1153334000
Brand: Merck
$288.00
Product Image of ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 10 L, This Product cannot be returned
ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 10 L, This Product cannot be returned
Item number: MC4809109010
Brand: Merck
$305.33
Product Image of ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 1 L, This Product cannot be returned
ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 1 L, This Product cannot be returned
Item number: MC4809101000
Brand: Merck
$72.00
Product Image of Solvent Cabinet for 1200 Infinity Series
Solvent Cabinet for 1200 Infinity Series
Item number: AG5065-9981
Brand: Agilent Technologies
$279.00
Product Image of Acetonitrile, 1 Liter
Acetonitrile, 1 Liter
Item number: AGG2453-85050
Brand: Agilent Technologies
$195.00

14 Items

Information about the different levels of purity for HPLC 

 

For modern HPLC high purity solutions as mobile phases are increasingly needed. Our consumers can mainly choose between three different classes of purity for their individual analysis, HPLC grade, Gradient grade and LC-MS grade.

But whats the difference between the different qualities referring chromatography and between other high purity solvents? And why is it important to choose a solvent with reference to the performed application?

All qualities have the same basic material and a nominal high purity with low traces of xenobiotics (99.8 - 99.9 %, depending on the product) in common. But especially these minimal residue can cause problems in HPLC depending in the technic of detection, so that in this case "pure" is not equal to "pure".

The following describes the differences of the three levels of solvent purity and the reasons for using every quality for your application. 

HPLC grade

HPLC grade describes a solutions quality, that is mainly desinged for isocratic separations applying UV and fluorescence detection. These solvents are especially characerized by high purity combined with high UV permeability at all relevant wave lengths, low inherent fluorescence and waste steam pressure as well as low acidity and alkalinity.

The solvents undergo special treatment stages to remove potentially interferring substances. Thereby disturbing peaks are minimized and a low ambient noise can be enabled.

After the treatment these solutions are tested specificly for aptitude for fluorescence detection in HPLC. Through this quality controll continous purity of solvents as well as reproducability for your measurements can be guaranteed.

Gradient grade

For chromatographical separation in HPLC besides isocratic also gradient elutions are often applied. At these composition of mobile phase doesn´t change wuring measurement., so e.g. of reversed phase chromatography the organic part increases. Using solvents, that are not specially designed for gradient separation, can cause a distinct increasing of the base line at UV detection and can be sensitive for sensitivity of the method.

To ensure optimal chromatography taking gradient into account Gradient grade is further cleaned up. Besides suitability for UV and fluorescence detection all solutions are specially tested for applicability for gradiental separation, so that a charge consistent quality can be guaranteed for Gradient grade solutions.

The following figure shows a gradient profile to demonstrade the extraordinary results that can be attained with acetonitril Gradient grade. Only a low drift of the base line can be recorded.

Fig. 1: Drift of the base line using Gradient grade as solvent. 

LC-MS grade

With the success of (ultra) high pressure liquid chromatography mass spectroscopy (UHPLC-MS) many new requirements arised to the solvent used because of high detection sensitivity.

This way the focus by using mass spectroscopy lies especially on high ionisation efficience with concurrent low ion background. Therefore the solvents are applicable cleaned up. Thereby removing trace elements, organics and (metall) ions has first priority. These can cause an increased ambient noise and adduct formation with the substances, that should be analysed. The result is a decreased detection sensability.

For quality controll all solvents are tested versus Reserpin (m/z 609.49) as standard. The following figure shows the scan of a positiv and negativ ESI and APCi modus. The whole tested mass area displays a low noise ambient as well as no signals cause by impurities.

Fig. 2: Noise ambient of LC-MS measurements with LC-MS grade solvents.

Here you can go back to the products.