Solvents for HPLC

High purity solvents for HPLC become increasingly important as mobile phase caused by increasing sensitivity of HPLC systems. Therefore the analytics-shop.com offers solutions with different levels of purity from J.T. Baker and Merck:

  HPLC grade Gradient grade LC-MS grade
Solutions  - 2-Propanol, Acetonitril
   Methanol, Water 
- Aceton, Dichlormethan,
   Isooctan, Tetrahydrofuran 
- 2-Propanol, Acetonitril,
   Methanol, Water 
Properties  - suitable for isocratic separations
- designed for UV or fluorescence
   detection

- low fluorescence, slight waste steam
   pressure

- low acidity and alkalinity 
- high UV permeability for UV-Vis
   and fluorescence measurements
- slight influence on the baselinie of UV
   detection
- suitable for highly sensitive measure-
   ments

- decreasing of trace minerals, organics
   and ions
- very low ambient noise
- suitable for for measurements with high
   sensitivity  

For more information about the levels of purity please click here.

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Product Image of Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Item number: MC1060072500
Brand: Merck
$192.00
Product Image of Acetonitrile hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Acetonitrile hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1000292500
Brand: Merck
$490.00
Product Image of Methanol hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Methanol hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1060352500
Brand: Merck
$197.00
Product Image of Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1153332500
Brand: Merck
$119.00
Product Image of Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L, can only be ordered in amounts of 4
Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L, can only be ordered in amounts of 4
Item number: MC1000142500
Brand: Merck
$173.00
Product Image of Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1000142511
Brand: Merck
$157.00
Product Image of Acetonitrile hypergrade for LC-MS LiChrosolv, 1 L
Acetonitrile hypergrade for LC-MS LiChrosolv, 1 L
Item number: MC1000291000
Brand: Merck
$235.00
Product Image of Ethanol denatured with about 1% methyl ethyl ketone for analysis EMSURE, 2,5 L
Ethanol denatured with about 1% methyl ethyl ketone for analysis EMSURE, 2,5 L
Item number: MC1009742511
Brand: Merck
$218.00
Product Image of Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1009832500
Brand: Merck
$267.00
Product Image of Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1009832511
Brand: Merck
$243.00
Product Image of Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
Item number: MC1060091011
Brand: Merck
$53.70
Product Image of Tetrahydrofuran for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Tetrahydrofuran for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1081012500
Brand: Merck
$489.00
Product Image of Ethanol gradient grade for liquid chromatography LiChrosolv, 2,5 L
Ethanol gradient grade for liquid chromatography LiChrosolv, 2,5 L
Item number: MC1117272500
Brand: Merck
$709.00
Product Image of Water for chromatography LiChrosolv, 1 L
Water for chromatography LiChrosolv, 1 L
Item number: MC1153331000
Brand: Merck
$57.00
Product Image of Water for analysis EMSURE, 10 L
Water for analysis EMSURE, 10 L
Item number: MC1167549010
Brand: Merck
$392.40
Product Image of Ethanol absolute EMPLURA, 2,5 L
Ethanol absolute EMPLURA, 2,5 L
Item number: MC8187602500
Brand: Merck
$146.00
Product Image of Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Acetone for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Item number: MC1000145000
Brand: Merck
$283.00
Product Image of Acetonitrile hypergrade for LC-MS LiChrosolv, 10 L
Acetonitrile hypergrade for LC-MS LiChrosolv, 10 L
Item number: MC1000299010
Brand: Merck
$2,014.00
Product Image of Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 4 L
Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 4 L
Item number: MC1000304000
Brand: Merck
$974.67
Product Image of Ethanol for spectroscopy Uvasol, 2,5 L
Ethanol for spectroscopy Uvasol, 2,5 L
Item number: MC1009802500
Brand: Merck
$450.00
Product Image of Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
Item number: MC1009831011
Brand: Merck
$116.00
Product Image of Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Item number: MC1009835000
Brand: Merck
$437.00
Product Image of Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 10 L
Ethanol absolute for analysis EMSURE ACS,ISO,Reag. Ph Eur, 10 L
Item number: MC1009836010
Brand: Merck
$939.60
Product Image of Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 1 L
Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 1 L
Item number: MC1060071000
Brand: Merck
$91.80
Product Image of Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1060092500
Brand: Merck
$123.00
Product Image of Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1060092511
Brand: Merck
$112.00
Product Image of Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Methanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Item number: MC1060095000
Brand: Merck
$202.00
Product Image of Methanol for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Methanol for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1060182500
Brand: Merck
$161.00
Product Image of Acetonitrile isocratic grade for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Acetonitrile isocratic grade for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1142912500
Brand: Merck
$359.00
Product Image of Solvent solvent for volumetric Karl Fischer titration with two component reagents apura, 1 L
Solvent solvent for volumetric Karl Fischer titration with two component reagents apura, 1 L
Item number: MC1880151000
Brand: Merck
$122.84
Product Image of Solvent solvent for volumetric Karl Fischer titration with two component reagents apura, 2,5 L
Solvent solvent for volumetric Karl Fischer titration with two component reagents apura, 2,5 L
Item number: MC1880152500
Brand: Merck
$252.56
Product Image of ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 1 L, This Product cannot be returned
ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 1 L, This Product cannot be returned
Item number: MC4809101000
Brand: Merck
$72.00
Product Image of ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 10 L, This Product cannot be returned
ETHANOL 20% FOR CLEANING OF BIOCHROMATOGRAPHY RESINS, 10 L, This Product cannot be returned
Item number: MC4809109010
Brand: Merck
$305.33
Product Image of Water for chromatography LiChrosolv, 4 L
Water for chromatography LiChrosolv, 4 L
Item number: MC1153334000
Brand: Merck
$288.00
Product Image of Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 1 L
Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 1 L
Item number: MC1037281002
Brand: Merck
$194.67
Product Image of Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 2 L
Water for UHPLC-MS,Lichrosolv,Supelco in borosilicate glass bottle, 2 L
Item number: MC1037282002
Brand: Merck
$296.00
Product Image of HPLC Flushing Solvent
HPLC Flushing Solvent
Item number: AGG1969-85026
Brand: Agilent Technologies
$163.00
Product Image of Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur
Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur
Item number: MC1060074000
Brand: Merck
$314.16
Product Image of Water with 0.1% (v/v) Acetic acid hypergrade for LC-MS LiChrosolv, 2500 ml
Water with 0.1% (v/v) Acetic acid hypergrade for LC-MS LiChrosolv, 2500 ml
Item number: MC1590072500
Brand: Merck
$108.67
Product Image of 1-Propanol for analysis EMSURE ACS,Reag. Ph Eur, 2,5 L
1-Propanol for analysis EMSURE ACS,Reag. Ph Eur, 2,5 L
Item number: MC1009972500
Brand: Merck
$308.00
Product Image of 2-Propanol gradient grade for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
2-Propanol gradient grade for liquid chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1010402500
Brand: Merck
$291.00
Product Image of 2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 1 L
Item number: MC1096341011
Brand: Merck
$81.90
Product Image of 2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 2,5 L
Item number: MC1096342511
Brand: Merck
$171.00
Product Image of 2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Item number: MC1096345000
Brand: Merck
$307.00
Product Image of 2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 10 L
2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 10 L
Item number: MC1096346010
Brand: Merck
$660.96
Product Image of 2-Propanol EMPLURA, 2,5 L
2-Propanol EMPLURA, 2,5 L
Item number: MC8187662500
Brand: Merck
$102.00
Product Image of Water solution, contains 10mmol ammonium acetate, CHROMASOLV(R), for HPLC, 4 x 4 L
Water solution, contains 10mmol ammonium acetate, CHROMASOLV(R), for HPLC, 4 x 4 L
Item number: SA667404-4X4L
Brand: Sigma-Aldrich
$591.00
Product Image of 2-Propanol for analysis EMSURE® ACS,ISO,Reag. Ph Eur, 25 L
2-Propanol for analysis EMSURE® ACS,ISO,Reag. Ph Eur, 25 L
Item number: MC1096346025
Brand: Merck
$1,177.20
Product Image of ACETONITRILE, FOR HPLC, GRADIENT GRADE,&
ACETONITRILE, FOR HPLC, GRADIENT GRADE,&
Item number: SA439134-18L
Brand: Sigma-Aldrich
$2,030.00
Product Image of ETHYLENE-D4 GLYCOL (OH,OD), 98 ATOM % D
ETHYLENE-D4 GLYCOL (OH,OD), 98 ATOM % D
Item number: SA347442-1G
Brand: Aldrich
$193.00

Information about the different levels of purity for HPLC 

 

For modern HPLC high purity solutions as mobile phases are increasingly needed. Our consumers can mainly choose between three different classes of purity for their individual analysis, HPLC grade, Gradient grade and LC-MS grade.

But whats the difference between the different qualities referring chromatography and between other high purity solvents? And why is it important to choose a solvent with reference to the performed application?

All qualities have the same basic material and a nominal high purity with low traces of xenobiotics (99.8 - 99.9 %, depending on the product) in common. But especially these minimal residue can cause problems in HPLC depending in the technic of detection, so that in this case "pure" is not equal to "pure".

The following describes the differences of the three levels of solvent purity and the reasons for using every quality for your application. 

HPLC grade

HPLC grade describes a solutions quality, that is mainly desinged for isocratic separations applying UV and fluorescence detection. These solvents are especially characerized by high purity combined with high UV permeability at all relevant wave lengths, low inherent fluorescence and waste steam pressure as well as low acidity and alkalinity.

The solvents undergo special treatment stages to remove potentially interferring substances. Thereby disturbing peaks are minimized and a low ambient noise can be enabled.

After the treatment these solutions are tested specificly for aptitude for fluorescence detection in HPLC. Through this quality controll continous purity of solvents as well as reproducability for your measurements can be guaranteed.

Gradient grade

For chromatographical separation in HPLC besides isocratic also gradient elutions are often applied. At these composition of mobile phase doesn´t change wuring measurement., so e.g. of reversed phase chromatography the organic part increases. Using solvents, that are not specially designed for gradient separation, can cause a distinct increasing of the base line at UV detection and can be sensitive for sensitivity of the method.

To ensure optimal chromatography taking gradient into account Gradient grade is further cleaned up. Besides suitability for UV and fluorescence detection all solutions are specially tested for applicability for gradiental separation, so that a charge consistent quality can be guaranteed for Gradient grade solutions.

The following figure shows a gradient profile to demonstrade the extraordinary results that can be attained with acetonitril Gradient grade. Only a low drift of the base line can be recorded.

Fig. 1: Drift of the base line using Gradient grade as solvent. 

LC-MS grade

With the success of (ultra) high pressure liquid chromatography mass spectroscopy (UHPLC-MS) many new requirements arised to the solvent used because of high detection sensitivity.

This way the focus by using mass spectroscopy lies especially on high ionisation efficience with concurrent low ion background. Therefore the solvents are applicable cleaned up. Thereby removing trace elements, organics and (metall) ions has first priority. These can cause an increased ambient noise and adduct formation with the substances, that should be analysed. The result is a decreased detection sensability.

For quality controll all solvents are tested versus Reserpin (m/z 609.49) as standard. The following figure shows the scan of a positiv and negativ ESI and APCi modus. The whole tested mass area displays a low noise ambient as well as no signals cause by impurities.

Fig. 2: Noise ambient of LC-MS measurements with LC-MS grade solvents.

Here you can go back to the products.