HPLC Inline-Filter

In addition to regular maintenance of the HPLC and UHPLC system it is recommended to use an inline-filter to protect the column and the plant. We offer  inline-filters of world-known manufacturers such as Agilent Technologies, Vici Jour and Upchurch Scientific.To keep HPLC and UHPLC systems free of contaminants that can damage the analytical column or even the entire system, the highest purity for all solutions that come into direct contact with the plant is necessary.  The housing of the inline-filter is made of PEEK or stainless steel with a frit in the interior and standard fittings at both ends. There are inline-filters, which are completely replaced if necessary, or a variant in which only the frit must be replaced.

If you have any questions while choosing a product, feel free to contact us.

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Product Image of Line Filter Assembly, equivalent product to Shimadzu 228-35871-96
Line Filter Assembly, equivalent product to Shimadzu 228-35871-96
Item number: 228-35871-96ASP
Brand: Altmann Analytik

In stock

$70.85
Product Image of In-Line precolumn Filter kit
In-Line precolumn Filter kit
Item number: WTWAT084560
Brand: Waters
$390.00
Product Image of Precolumn MicroFilter, 0.5µm, PEEK, for 1/16'' OD, with 5 PEEK frits, 1pc/PAK
Precolumn MicroFilter, 0.5µm, PEEK, for 1/16'' OD, with 5 PEEK frits, 1pc/PAK
Item number: UPM-560
Brand: Upchurch Scientific

In stock

$218.30
Product Image of Precolumn Filter, PEEK, 2µm, 1pc/PAK
Precolumn Filter, PEEK, 2µm, 1pc/PAK
Item number: UPA-355
Brand: Upchurch Scientific

In stock

$118.27
Product Image of Precolumn Filter Assy 2µm, 1pc/PAK
Precolumn Filter Assy 2µm, 1pc/PAK
Item number: UPA-315
Brand: Upchurch Scientific

In stock

$123.06
Product Image of Precolumn Filter Insert Assembly
Precolumn Filter Insert Assembly
Item number: WTWAT088084
Brand: Waters
$27.00
Product Image of Line Filter, SS, for Shimadzu model LC-30AD
Line Filter, SS, for Shimadzu model LC-30AD
Item number: 228-35871-99ASP
Brand: Altmann Analytik
$152.77
Product Image of Inline Filter, 0,5 µm, PEEK, kit, Chip Cube LC system
Inline Filter, 0,5 µm, PEEK, kit, Chip Cube LC system
Item number: AG5067-1582
Brand: Agilent Technologies
$474.96
Product Image of Inline Filter, SS, 0,2 µm, 2, 1 mm, for RRLC, con. Capill.
Inline Filter, SS, 0,2 µm, 2, 1 mm, for RRLC, con. Capill.
Item number: AG5067-1551
Brand: Agilent Technologies
$293.29
Product Image of Chemical Trap, with 4 O-RIngs, 2 pc/PAK
Chemical Trap, with 4 O-RIngs, 2 pc/PAK
Item number: AGG1544-80530
Brand: Agilent Technologies
$157.00
Product Image of Frit, for Agilent 1290 In-line Filter, 0.3 µm, 5 pc/PAK
Frit, for Agilent 1290 In-line Filter, 0.3 µm, 5 pc/PAK
Item number: 5023-0271ASP
Brand: Altmann Analytik
$134.00
Product Image of Guard Column Filter Kit Filter L-column, 10-32 Thread, Type Waters/Waters, include Holder and Frit
Guard Column Filter Kit Filter L-column, 10-32 Thread, Type Waters/Waters, include Holder and Frit
Item number: CILC653002
Brand: CERi
$277.00 $277.03
Product Image of Guard Column Filter Kit L-column, 10-32 Thread, Type Waters/UPLC®, include Holder and Frit
Guard Column Filter Kit L-column, 10-32 Thread, Type Waters/UPLC®, include Holder and Frit
Item number: CILC653004
Brand: CERi
$277.00 $277.03
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 0.2µm, 10/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 0.2µm, 10/PAK
Item number: OZ150405264
Brand: Optimize
$1,280.92
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 0.2µm, 5/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 0.2µm, 5/PAK
Item number: OZ150405263
Brand: Optimize
$680.23
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 0.5µm, 10/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 0.5µm, 10/PAK
Item number: OZ150405262
Brand: Optimize
$1,280.92
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 0.5µm, 5/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 0.5µm, 5/PAK
Item number: OZ150405261
Brand: Optimize
$680.23
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 2µm, 10/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 2µm, 10/PAK
Item number: OZ150405260
Brand: Optimize
$1,280.92
Product Image of EXP2 Pre-Column Filter Cartridge, 3 mm, 2µm, 5/PAK
EXP2 Pre-Column Filter Cartridge, 3 mm, 2µm, 5/PAK
Item number: OZ150405259
Brand: Optimize
$680.23
Product Image of Optimize EXP Stem Filter Kit 0.5µm
Optimize EXP Stem Filter Kit 0.5µm
Item number: OZ150404123
Brand: Optimize
$732.35
Product Image of Optimize EXP Stem Filter Kit 0.2µm
Optimize EXP Stem Filter Kit 0.2µm
Item number: OZ150404122
Brand: Optimize
$732.35
Product Image of Optimize EXP Pre-Column Filter Cartridge, 0.2µm, 10/PAK
Optimize EXP Pre-Column Filter Cartridge, 0.2µm, 10/PAK
Item number: OZ150403097
Brand: Optimize
$1,197.25
Product Image of Optimize EXP Pre-Column Filter Cartridge, 0.2µm, 5/PAK
Optimize EXP Pre-Column Filter Cartridge, 0.2µm, 5/PAK
Item number: OZ150403096
Brand: Optimize
$636.35
Product Image of Optimize EXP Pre-Column Filter Cartridge, 0.5µm, 10/PAK
Optimize EXP Pre-Column Filter Cartridge, 0.5µm, 10/PAK
Item number: OZ150403094
Brand: Optimize
$1,197.25
Product Image of Optimize EXP Pre-Column Filter Cartridge, 0.5µm, 5/PAK
Optimize EXP Pre-Column Filter Cartridge, 0.5µm, 5/PAK
Item number: OZ150403093
Brand: Optimize
$636.35
Product Image of Filter (Last Drop) with 5µm Pat Filter
Filter (Last Drop) with 5µm Pat Filter
Item number: WTPSL901294
Brand: Waters
$116.00
Product Image of Filter (Last Drop) with 2µm Pat Filter
Filter (Last Drop) with 2µm Pat Filter
Item number: WTPSL901292
Brand: Waters
$158.00
Product Image of Filter (Last Drop) with 10µm SS Filter
Filter (Last Drop) with 10µm SS Filter
Item number: WTPSL901291
Brand: Waters
$106.00
Product Image of Filter (Last Drop) with, 2µm SS Filter
Filter (Last Drop) with, 2µm SS Filter
Item number: WTPSL901290
Brand: Waters
$106.00
Product Image of Filter, PEEK, In-Line, complete, frit Ti PEEK-encased 5 um
Filter, PEEK, In-Line, complete, frit Ti PEEK-encased 5 um
Item number: JR-68256
Brand: Vici Jour
$112.13
Product Image of Sure-Guard, disposable In-Line Filter, TI frit 2.0 um, minimum order amount = 4 pcs
Sure-Guard, disposable In-Line Filter, TI frit 2.0 um, minimum order amount = 4 pcs
Item number: JR-0611-TI2
Brand: Vici Jour
$36.40
Product Image of Sure-Guard, disposable In-Line Filter, TI frit 0.5 um, minimum order amount = 4 pcs
Sure-Guard, disposable In-Line Filter, TI frit 0.5 um, minimum order amount = 4 pcs
Item number: JR-0611-TI05
Brand: Vici Jour
$36.40
Product Image of Sure-Guard, disposable In-Line Filter,SS frit 2.0 um, minimum order amount = 4 pcs
Sure-Guard, disposable In-Line Filter,SS frit 2.0 um, minimum order amount = 4 pcs
Item number: JR-0611-SS2
Brand: Vici Jour
$36.40
Product Image of Sure-Guard, disposable In-Line Filter,SS frit 0.5 um, minimum order amount = 4 pcs
Sure-Guard, disposable In-Line Filter,SS frit 0.5 um, minimum order amount = 4 pcs
Item number: JR-0611-SS05
Brand: Vici Jour
$36.40
Product Image of Precolumn MicroFilter, 0.5µm, PEEK, for 1/16'' OD, with 1 PEEK frit, 1pc/PAK
Precolumn MicroFilter, 0.5µm, PEEK, for 1/16'' OD, with 1 PEEK frit, 1pc/PAK
Item number: UPM-560-1
Brand: Upchurch Scientific
$480.62
Product Image of Precolumn MicroFilter, 2µm, for 1/16'' OD, with 1 SSfrit, 1pc/PAK
Precolumn MicroFilter, 2µm, for 1/16'' OD, with 1 SSfrit, 1pc/PAK
Item number: UPM-555-1
Brand: Upchurch Scientific
$188.09
Product Image of SemiPrep Filter, 5µm, for 3/16'' OD, 1pc/PAK
SemiPrep Filter, 5µm, for 3/16'' OD, 1pc/PAK
Item number: UPA-510
Brand: Upchurch Scientific
$223.99
Product Image of Inline Filter Assembly, 0.5µm, 1pc/PAK
Inline Filter Assembly, 0.5µm, 1pc/PAK
Item number: UPA-431
Brand: Upchurch Scientific
$102.16
Product Image of Inline Filter Assembly, 2µm, 1pc/PAK
Inline Filter Assembly, 2µm, 1pc/PAK
Item number: UPA-430
Brand: Upchurch Scientific
$67.62
Product Image of Inline Filter, metal free, 10µm, for 1/16'' OD, 1pc/PAK
Inline Filter, metal free, 10µm, for 1/16'' OD, 1pc/PAK
Item number: UPA-425
Brand: Upchurch Scientific
$39.36
Product Image of Low Pressure Inline Filter, 2 µm, for 1/8'' OD, 1pc/PAK
Low Pressure Inline Filter, 2 µm, for 1/8'' OD, 1pc/PAK
Item number: UPA-416
Brand: Upchurch Scientific
$54.60
Product Image of Low Pressure Inline Filter, 2 µm, for 1/16'' OD, minimum order amount 5 pcs.
Low Pressure Inline Filter, 2 µm, for 1/16'' OD, minimum order amount 5 pcs.
Item number: UPA-415
Brand: Upchurch Scientific
$54.60
Product Image of SemiPrep Inline Filter Assembly, 10µm, 1pc/PAK
SemiPrep Inline Filter Assembly, 10µm, 1pc/PAK
Item number: UPA-411
Brand: Upchurch Scientific
$217.55
Product Image of SemiPrep Inline Filter Assembly, 2µm, 1pc/PAK
SemiPrep Inline Filter Assembly, 2µm, 1pc/PAK
Item number: UPA-410
Brand: Upchurch Scientific
$217.55
Product Image of SemiPrep Filter Assembly, 10µm, 1pc/PAK
SemiPrep Filter Assembly, 10µm, 1pc/PAK
Item number: UPA-360
Brand: Upchurch Scientific
$388.31
Product Image of Precolumn Filter, PEEK, 0.5µm, 1pc/PAK
Precolumn Filter, PEEK, 0.5µm, 1pc/PAK
Item number: UPA-356
Brand: Upchurch Scientific
$118.81
Product Image of Filter Cap Precolumn Biocomp, 1pc/PAK
Filter Cap Precolumn Biocomp, 1pc/PAK
Item number: UPA-355-02
Brand: Upchurch Scientific
$51.47
Product Image of Filter Body Precolumn Biocomp, 1pc/PAK
Filter Body Precolumn Biocomp, 1pc/PAK
Item number: UPA-355-01
Brand: Upchurch Scientific
$51.95
Product Image of SemiPrep Filter Assembly, 10 µm, 1pc/PAK
SemiPrep Filter Assembly, 10 µm, 1pc/PAK
Item number: UPA-330
Brand: Upchurch Scientific
$213.62
Product Image of Precolumn Filter, 0.5µm, 0, 84µl swept volume, 1pc/PAK
Precolumn Filter, 0.5µm, 0, 84µl swept volume, 1pc/PAK
Item number: UPA-318
Brand: Upchurch Scientific
$159.31

Use of inline filters in chromatography

Impurities can not only get into the system from outside but also from the system itself. The main sources are mainly the piston seals the pump or the rotor seal the injection valve, which can produce abrasion with increasing operating time. This abrasion can lead to deposits / blockage on the stationary phase or in the HPLC system.

In addition to regular maintenance of the system it is recommended to use an inline filter to protect the column and the plant. But what is the inline filter and why makes its use a lot of sense? And what is important in choosing the right inline filter?

The in-line filter can be positioned at various locations of the HPLC system. If the inline filter is installed between the pump and the injector, particles and impurities are removed from the eluent. Likewise, abrasion of the piston seal is intercepted, so that the rotor seal of the injection valve is protected from abrasion and fine particles. When positioning directly in front of the column after the injection valve and contaminants from the sample are removed and abrasion of the rotor seals.

In addition to the inline filter it is usually recommended to use a guard column. Molecules that can be attached irreversibly to the main column can not be retained with the inline filter. Inline filters are available in various designs. In addition to housing, which is either made of PEEK or stainless steel can also choose between different fittings. These depend on the place where the inline filter should be installed. Thus, there are also housing, which are provided for the direct connection to the analytical column.

Accessories for the inline filters: frits

The frits for the inline filters are usually made of stainless steel and are often surrounded by a ring of PEEK. This serves as a seal ring, while minimizing the dead volume of the inline filter, so that no negative effects such as peak broadening can be expected. Stainless steel frits can be used for the current analysis. For applications requiring high corrosion resistance and biocompatibility, frits made of other materials such as Titanium or polymers are available. Also, the surrounding ring can be made of other materials, such as of PTFE. The user then has the possibility to choose a matching inline filter depending on the sample to be analyzed and the chemical properties of the mobile phase.

Typical pore sizes of the frits are 2 and 0.5 microns. When using columns with a particle size of <5 micron, frits with 0.5 microns should be used. For UHPLC systems and columns with particle sizes below 2 microns using frits with a pore size of 0.2 microns is recommended. The maximum pressure is 1000 bar and they have a low dead volume.

In the long term, the use of inline filters in the HPLC analysis has many advantages and saves money. Maintenance costs will be reduced and the facility and the separation column with usage of the inline filter will guarantee an extended service life.