Find the right column from over 120,000 HPLC columns from more than 30 different manufacturers. We also offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative. Compare prices and the application possibilities of the various manufacturers. If you are unable to find a suitable HPLC column, please feel free to contact us.
Prior to the development of the Reversed Phase (RP), Normal Phase Chromatography was the most common separation mode. We offer Normal Phase (NP) HPLC columns by all well-known manufacturers here.
In Normal Phase Chromatography, the mobile phase is non-polar and the stationary phase is polar (common: silica gel). This technique relies on the interaction of analytes with polar functional groups on the surface of the stationary phase. This interaction is strongest when using non-polar solvents as the mobile phase. The least polar compounds elute first and the most polar compounds elute last.
Normal Phase Chromatography is a very effective separation method because a wide range of solvents can be used to fine-tune the selectivity of a separation. However, it has become less popular with many chromatographers because of the complexity involved. Under certain conditions, long equilibration times or reproducibility problems may occur. This is due mainly to the sensitivity of the technique to the presence of low concentrations of polar contaminants in the mobile phase. Controlling these problems effectively produces better chromatograms than reverse phase methods as the commonly used solvents have a lower viscosity.
In Reversed Phase Chromatography, the polarity of the phases are "reversed". The mobile phase is polar and the stationary phase is non-polar. As it is one of the most popular separation modes in chromatography, all related products of all well-known manufacturers are available here.
The non-polar side chains in Reversed Phase Chromatography columns are bound either to a polymer or to a structure made of silica gel, which leads to them being hydrophobic. The longer the chain, the more non-polar the phases are. Polar analytes are eluted from the column first, followed by the non-polar analytes. Reversed phase is more widely practised than normal phased as they can be used universally for polar and non-polar analytes. In addition, this method is very sensitive and flexible as small changes in the composition of the mobile phase (e.g. salts, pH, organic solvents) or the temperature can completely change the separation properties of the system. RP-HPLC is used especially in numerous applications of UV spectroscopy (LC-UV).
Unlike normal HPLC columns, chiral or enantiomeric HPLC can also be used to separate and determine chiral compounds. This requires special chiral stationary phases that have fixed chiral functionalities. In the Analytics Shop, we have over 1,400 different chiral columns from different manufacturers, including the high-quality columns from Chiral Technologies as well as lower-priced alternatives of the same quality from YMC and our own Altmann Analytik brand.
Special columns such as chiral or enantiomeric HPLC columns are required to separate chiral compounds. With suitable interaction, these enable the separation of the enantiomers which have almost identical physical and chemical properties. Enantiomers are molecules with a chiral center. Diastereomers are molecules with two or more chiral centers, differ in their chemical properties and can be separated using chromatography. The separation of enantiomers is based on the formation of diastereomers and their interaction with other chiral molecules like chiral stationary phases (e.g. silica gels) in chromatographic separations. A conventional solvent is used as the eluent.
With Ultra High Performance Liquid Chromatography (UHPLC), dramatic increases in resolution, speed and sensitivity of HPLC can be achieved by using short and thin columns. The particles of the filling material have diameters of less than 2 µm, which results in an improved separation performance compared to standard HPLC. The increased total surface area of the filling material gives the analyte a higher adsorption ability. Analysis times are reduced due to the shorter columns and less solvent is required.
Hydrophilic interaction chromatography (HILIC) is a popular alternative to normal phase and reverse phase chromatography. Similar to NP, buffer systems are used as stationary phases in HILIC mode. These are aqueous buffer systems with organic solvents, e.g. B. acetonitrile. In HILIC mode, water is the strongest eluant. HILIC columns are suitable for the separation of polar compounds such as carbohydrates, polar metabolites and hydrophilic compounds regardless of charge and molecular size. An extensive range of HILIC columns from established quality manufacturer YMC is available in our shop.
Significant advantages of Supercritical Liquid Chromatography (SFC) columns compared to HPLC columns:
Supercritical fluids show low viscosities and higher diffusivities when used as a mobile phase, resulting in narrower peaks due to rapid diffusion and faster elution and less pressure drop through the column. Supercritical fluids combine the benefits of liquids and gases, hence enabling the SFC technique to combine the best aspects of HPLC and gas chromatography (GC). In most cases, a supercritical fluid such as carbon dioxide is used as the mobile phase. The low viscosity of supercritical carbon dioxide enables analytical separations that are 3-5 times faster than those for normal phase HPLC. The speed of SFC separations, the preservation of organic solvents and more concentrated product fractions make SFC a preferred chromatographic technique for separating and purifying chemical mixtures.
SFC is an environmentally friendly separation technique that makes use of CO2-based mobile phases. The use of high-performance preparative columns (internal diameter of 10 - 50 mm) with a large number of particle sizes from 3 - 20 μm leads to the quick separation and recovery of cleaned components.
Nano HPLC uses columns with very small inner diameters. Columns with internal diameters of 75 µm, 100 µm or 150 µm are commonly used. Due to the reduction in the internal diameter, injection and flow rates must be reduced which is particularly advantageous when only small or diluted sample quantities are available. The smaller nano HPLC columns lead to an increased sensitivity with lower solvent consumption. Nano columns are able to maintain a high concentration of the injected sample and to direct approx. 40-50 % of the sample to the detector. Nano HPLC has a high separation efficiency compared to the traditional HPLC technique.
Gel permeation chromatography (GPC) is particularly suitable for the separation of non-polar molecules and can be used for a wide range of solvents, from non-polar organics to aqueous applications. GPC/SEC columns are packed with very small porous beads. In GPC/SEC, the molecules are separated according to size (size exclusion chromatography, SEC). The smaller molecules can enter the pores more easily and therefore spend more time in these pores, eluting last. Conversely, larger molecules spend little if any time in the pores and are eluted quickly. The GPC columns are filled with a microporous packing material and gel, hence the name gel permeation.
Ion exchange chromatography (IEC) separates molecules based on the respective charged groups on the surface of a protein. Molecules undergo electrostatic interactions wirh opposite charges on the stationary phase matrix.
The pH value of the buffer, the buffer concentration, packing of the columns and the salt gradient, in which the salt ions compete with the desired proteins to bind to charged groups on the surface of the medium, are important aspects affecting the results of IEC. At any pH, a protein has a net positive or negative charge due to the state of charge of the amino acids. If this protein is positively charged, it will bind to negatively charged materials and vice versa. The separation is based essentially on the charge and size of the ions.
Resins such as polystyrene, cellulose and crosslinked polyacrylamide or polydextran gels are used as mediums for ion exchange. The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged and anion-exchange chromatography is when the stationary phase is positively charged. Ion exchange chromatography is usually performed at pH values in which the protein has a net charge opposite to that of the stationary phase matrix.
Polar-bound phases are based on silica gels which have chains attached onto the silica structure with functional groups. Chromatography makes use of the difference in polarity to separate a mixture. Separation takes place through different mechanisms and often as a result of the combination of several processes like adsorption, distribution, ion exchange, molecular size exclusion etc.
The U.S. Pharmacopeia Convention is a scientific non-profit company that sets the standards for ingredients, concentration, quality and purity of medicines, food ingredients and food supplements that are manufactured, distributed and consumed all over the world. According to USP regulations, the following deviations may occur:
USP Chapter
European Pharmacopeia Ph. Eur., Chapter 2.2.46
The European Pharmacopeia is a published collection of monographs describing the individual and general quality standards of ingredients, dosages and analytical methods of medicine. The aim is to set common quality standards across Europe to control the quality of medicines and other chemical products. According to the EP regulation, the following deviations can occur:
Isocratic elution
Gradient elution
| Phase Name | USP Number | Possible Materials |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Altmann Reprosil Pur C18-AQ, 5μm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Altmann Reprosil 80 ODS-2, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Merck LiChrospher RP-18, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Merck Purospher Star RP-18, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Waters Spherisorb ODS-2, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Waters Symmetry C18, 5µm |
| Porous silica gel, 5 to 10 μm particle size | L3 | Altmann Reprosil-Pur Si |
| Porous silica gel, 5 to 10 μm particle size | L3 | Altmann Reprosil 80 Si |
| Porous silica gel, 5 to 10 μm particle size | L3 | Merck LiChrospher Si 60, 5µm |
| Porous silica gel, 5 to 10 μm particle size | L3 | Merck Chromolith Performance Si 100, 4.6mm |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Altmann Reprosil -Pur Basic C8 (HD) |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Altmann Reprospher C8 (DE) |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Waters Symmetry C8 |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Waters XBridge C8 |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Merck Purospher STAR RP-8 Endcapped 5µm |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Merck Chromolith Performance RP-8 endc., 4,6mm |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Altmann Reprosil 100 NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Altmann Reprosil-Pur NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Waters µBondapak NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Waters Spherisorb NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Merck LiChrospher 100 NH2, 5µm |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Merck Purospher STAR NH2, 5µm |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Altmann Reprosil 80 SCX |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Altmann Reprosil Saphir SCX |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Waters Spherisorb SCX |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Reprosil 100 CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Reprosil 80 CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Equisil CPS |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Waters µBondapak CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Waters Spherisorb CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Merck LiChrospher 100CN, 5µm |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Altmann Reprosil 100 Phenyl |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Altmann Reprosil 80 Phenyl |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Waters XBridge Phenyl 5µm |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Waters XTerra Phenyl 5µm |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Altmann Reprosil-Pur C1 |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Altmann Reprosil 80 C1 |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Waters Spherisorb C1 5µm |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Waters Spherisorb C1 3µm |
| Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger, 5 to 10 μm particle size | L14 | Altmann Reprosil 80 SAX |
| Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger, 5 to 10 μm particle size | L14 | Waters Spherisorb SAX 5µm |
| Methylsilane groups chemically bound to completely porous silica gel, 3 to 10 μm particle size | L15 | Altmann Reprosil 80 C6 |
| Methylsilane groups chemically bound to completely porous silica gel, 3 to 10 μm particle size | L15 | Waters Spherisorb C6 5µm |
| Dimethylsilane chemically bound to porous silica gel, 3 to 10 μm particle size | L16 | Altmann Reprosil Gold 120 C2 |
| Dimethylsilane chemically bound to porous silica gel, 3 to 10 μm particle size | L16 | Altmann Reprosil Gold 300 C2 |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form, 7 to 11 μm particle size | L17 | Waters IC-pak cation |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form, 7 to 11 μm particle size | L17 | Waters IC-pak ion exclusion |
| Amino and cyano groups chemically bound to porous silica gel, 3 to 10 μm particle size | L18 | Altmann Repro-Gel H |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form, 9μm particle size | L19 | Altmann Reprogel Ca2+ |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form, 9μm particle size | L19 | Waters Sugar-Pak 1 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil 100 Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil-Pur Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil 80 Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Waters Protein-Pak 60 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Waters BioSuite 250 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Merck LiChrospher 100 Diol, 5µm |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 100 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 300 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 1000 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Waters Styragel HR4E |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Shodex Shodex RSpak 613 |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Altmann Repromer SCX |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Waters IC-Pak Ion exclusion |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Shodex Shodex SP-0810 |
| Ion exchange resin of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, approximately 10 μm particle size | L23 | Shodex Shodex IEC QA-825 |
| Pack with the ability to separate compounds (in a molecular weight range of 100 to 5000 daltons (determined with polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Polymethacrylic resin cross-linked with polyhydroxilated ether (surface containing residual carboxyl group content) was found to be appropriate | L25 | Shodex Shodex OHpak SB-802 HQ |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil 100 C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil-Pur C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil Gold C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Waters Acquity UPLC BEH 300 C4 1.7µm |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Waters Symmetry 300 C4 |
| Chiral ligand exchange material with L-proline copper complex covalently bound to broken silica gel, 5 to 10 μm particle size | L32 | Altmann Reprosil Chiral-L-Prolin |
| Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form, 9μm particle size | L34 | Altmann Reprogel Pb 9µm |
| Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form, 9μm particle size | L34 | Shodex Shodex SP0810 |
| Polymethacrylate gel pack with the ability to separate proteins in a molecular weight range between 2,000 and 40,000 daltons by molecular size. | L37 | Shodex Shodex OHpak SB-803HQ |
| Size exclusion Pack for water soluble paints based on methacrylate | L38 | Shodex Shodex OHpak SB-802 HQ |
| Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin | L39 | Shodex Shodex OHpak SB-802 HQ |
| Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin | L39 | Shodex Shodex RSpak DM-614 |
| Cellulose tris-3,5-dimethylphenylcarbamate on porous silica gel, 5 to 20 μm particle size | L40 | Altmann Reprosil Chiral-OM |
| Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel, 5 μm particle size | L41 | Altmann Reprosil-AGP |
| Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel, 5 μm particle size | L41 | Chiral Chiral-AGP |
| Pentafluorophenyl groups are chemically bound to silica gel, 5 to 10 μm particle size | L43 | Altmann Reprosil Fluosil PFP |
| Pentafluorophenyl groups are chemically bound to silica gel, 5 to 10 μm particle size | L43 | Waters XSelect CSH Fl-Ph 5µ |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Altmann RCX-30 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton PRP-X110 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton RCX-10 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton RCX-30 |
| Amylose tris-3,5-dimethylphenylcarbamate on porous, spherical silica gel, 5 to 10 μm particle size | L51 | Altmann Reprosil Chiral-AM |
| Ovomucoid (chiral recognition protein). Chemically bound to silica particles, approximately 5μm particle size, 120 angstrom pore size | L57 | Agilent Ultron ES-OVM |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the sodium (Na +) form, 7 to 11 μm particle size | L58 | Altmann Reprogel Na+ |
| Spherical, porous silica gel with a covalent surface modification with alkylamide groups with end capping, 3-5 μm particle size | L60 | Altmann Reprosil ABZ-Amid C18 |
| C30 silane is bound to a completely porous silica gel, 3 to 15 μm | L62 | Altmann Stability C30 |
