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HPLC Column Configurator
Find & Compare HPLC Columns
Here you can find the right column for your separation problem from around 30.000 HPLC columns by more than 10 different manufacturers. Compare the prices and application possibilities of the various suppliers. We carry HPLC columns from well-known manufacturers. As a good alternative we supply you with our brand Altmann Analytik HPLC columns which offers you the best quality at lower costs. If you can't find the right column, please don't hesitate to contact us.
HPLC columns in various specifications
Our HPLC column configurator helps you find the desired column quickly and easily. It allows you to filter out the wanted HPLC columns from all well-known manufacturers in just a few clicks. For use in the GLP/GMP regulated environment and in the pharmaceutical industry our products are marked with the universal USP code. Among other things you can select the following specifications freely:
- Material: Amino, C8, C18, Cyano, Diol, Phenyl, Silica etc.
- Pore Size: 25 A - 10000 A
- Particle Size: 1.5 µm - 60 µm
- Manufacturer: Agilent, Grace, Macherey-Nagel, Merck, Chiral, etc.
Permitted changes according to USP (United States Pharmacopeia)
The U.S. Pharmacopeia Convention is a scientific non-profit company that sets the standards for ingredients, concentration, quality and purity of medicines, food ingredients and food supplements that are manufactured, distributed and consumed all over the world. According to USP regulations, the following deviations may occur:
USP Chapter
- Column Length: ± 70%
- Column Inner Diameter: can be changed if the flow rate is kept constant.
- Particle Size: - 50%
- Flow Rate: ± 50%
- Ratio of components in the mobile phase: ± 30% or ± 10%
- pH value of the mobile phase: ± 0,2
- Salt concentration in the buffer: ± 10%
- Column Temperature: ± 10°C
- Wave Length of the UV-Vis detector: ± 3 nm
- Injection Volume: can be reduced until precision and detection limits are reached.
Permitted changes according to EP (European Pharmacopeia)
European Pharmacopeia Ph. Eur., Chapter 2.2.46
The European Pharmacopeia is a published collection of monographs describing the individual and general quality standards of ingredients, dosages and analytical methods of medicine. The aim is to set common quality standards across Europe to control the quality of medicines and other chemical products. According to the EP regulation, the following deviations can occur:
Isocratic elution
- Column Length: ± 70%
- Column Inner Diameter: ± 25%
- Particle Size: - 50%
- Flow Rate: ± 50%
- Ratio to components in the mobile phase: ± 30% or ± 2% absolute
- pH value of the mobile phase: ±0,2
- Salt concentration in the buffer: ± 10%
- Column Temperature: ± 10°C
- Wave Lenght of the detector: ± 3 nm
- Injection Volume: can be reduced until precision and detection limits are reached.
Gradient elution
- Column Length: ± 70%
- Column Inner Diameter: ± 25%
- Particle Size: no changes permitted
- Flow Rate: changes acceptable if column size is changed
- Ratio to components in the mobile phase: small changes in the composition of the mobile phase and the (density) grade are acceptable if the system configuration still meets the requirements.
- Dwell Volume: gradient instants can be used to compensate for differences in dwell volume between different systems.
- pH value of the mobile phase: no changes permitted
- Salt concentration in the buffer: no changes permitted
- Column Temperature: ± 5°C
- Wave Lenght of the detector: no deviations permitted
- Injection Volume: can be reduced until precision and detection limits are reached.
| Phase Name | USP Number | Possible Materials |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Altmann Reprosil Pur C18-AQ, 5μm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Altmann Reprosil 80 ODS-2, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Merck LiChrospher RP-18, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Merck Purospher Star RP-18, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Waters Spherisorb ODS-2, 5µm |
| Octadecylsilane chemically bound to porous silica gel, 1.8 to 10 μm particle size | L1 | Waters Symmetry C18, 5µm |
| Porous silica gel, 5 to 10 μm particle size | L3 | Altmann Reprosil-Pur Si |
| Porous silica gel, 5 to 10 μm particle size | L3 | Altmann Reprosil 80 Si |
| Porous silica gel, 5 to 10 μm particle size | L3 | Merck LiChrospher Si 60, 5µm |
| Porous silica gel, 5 to 10 μm particle size | L3 | Merck Chromolith Performance Si 100, 4.6mm |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Altmann Reprosil -Pur Basic C8 (HD) |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Altmann Reprospher C8 (DE) |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Waters Symmetry C8 |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Waters XBridge C8 |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Merck Purospher STAR RP-8 Endcapped 5µm |
| Octylsilane was chemically bound to completely porous silica gel, 1.8 to 10 μm particle size | L7 | Merck Chromolith Performance RP-8 endc., 4,6mm |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Altmann Reprosil 100 NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Altmann Reprosil-Pur NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Waters µBondapak NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Waters Spherisorb NH2 |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Merck LiChrospher 100 NH2, 5µm |
| A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel, 3 to 10 μm particle size | L8 | Merck Purospher STAR NH2, 5µm |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Altmann Reprosil 80 SCX |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Altmann Reprosil Saphir SCX |
| Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger, 3 to 10 μm particle size | L9 | Waters Spherisorb SCX |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Reprosil 100 CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Reprosil 80 CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Altmann Equisil CPS |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Waters µBondapak CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Waters Spherisorb CN |
| Nitrile groups chemically bound to porous silica gel, 3 to 10 μm particle size | L10 | Merck LiChrospher 100CN, 5µm |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Altmann Reprosil 100 Phenyl |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Altmann Reprosil 80 Phenyl |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Waters XBridge Phenyl 5µm |
| Phenyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L11 | Waters XTerra Phenyl 5µm |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Altmann Reprosil-Pur C1 |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Altmann Reprosil 80 C1 |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Waters Spherisorb C1 5µm |
| Trimethyl groups chemically bound to porous silica gel, 3 to 10 μm particle size | L13 | Waters Spherisorb C1 3µm |
| Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger, 5 to 10 μm particle size | L14 | Altmann Reprosil 80 SAX |
| Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger, 5 to 10 μm particle size | L14 | Waters Spherisorb SAX 5µm |
| Methylsilane groups chemically bound to completely porous silica gel, 3 to 10 μm particle size | L15 | Altmann Reprosil 80 C6 |
| Methylsilane groups chemically bound to completely porous silica gel, 3 to 10 μm particle size | L15 | Waters Spherisorb C6 5µm |
| Dimethylsilane chemically bound to porous silica gel, 3 to 10 μm particle size | L16 | Altmann Reprosil Gold 120 C2 |
| Dimethylsilane chemically bound to porous silica gel, 3 to 10 μm particle size | L16 | Altmann Reprosil Gold 300 C2 |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form, 7 to 11 μm particle size | L17 | Waters IC-pak cation |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form, 7 to 11 μm particle size | L17 | Waters IC-pak ion exclusion |
| Amino and cyano groups chemically bound to porous silica gel, 3 to 10 μm particle size | L18 | Altmann Repro-Gel H |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form, 9μm particle size | L19 | Altmann Reprogel Ca2+ |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form, 9μm particle size | L19 | Waters Sugar-Pak 1 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil 100 Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil-Pur Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Altmann Reprosil 80 Diol |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Waters Protein-Pak 60 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Waters BioSuite 250 |
| Dihydroxypropane groups chemically bound to porous silica gel, 5 to 10 μm particle size | L20 | Merck LiChrospher 100 Diol, 5µm |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 100 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 300 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Altmann Repromer 1000 RPS |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Waters Styragel HR4E |
| Stable, spherical styrene-divinylbenzene copolymer, 5 to 10 μm particle size | L21 | Shodex Shodex RSpak 613 |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Altmann Repromer SCX |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Waters IC-Pak Ion exclusion |
| A cation exchange resin of porous polystyrene having sulfonic acid groups, about 10 μm in particle size | L22 | Shodex Shodex SP-0810 |
| Ion exchange resin of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, approximately 10 μm particle size | L23 | Shodex Shodex IEC QA-825 |
| Pack with the ability to separate compounds (in a molecular weight range of 100 to 5000 daltons (determined with polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Polymethacrylic resin cross-linked with polyhydroxilated ether (surface containing residual carboxyl group content) was found to be appropriate | L25 | Shodex Shodex OHpak SB-802 HQ |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil 100 C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil-Pur C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Altmann Reprosil Gold C4 |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Waters Acquity UPLC BEH 300 C4 1.7µm |
| Methylsilane is chemically bound to completely porous silica gel, 5 to 10 μm particle size | L26 | Waters Symmetry 300 C4 |
| Chiral ligand exchange material with L-proline copper complex covalently bound to broken silica gel, 5 to 10 μm particle size | L32 | Altmann Reprosil Chiral-L-Prolin |
| Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form, 9μm particle size | L34 | Altmann Reprogel Pb 9µm |
| Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form, 9μm particle size | L34 | Shodex Shodex SP0810 |
| Polymethacrylate gel pack with the ability to separate proteins in a molecular weight range between 2,000 and 40,000 daltons by molecular size. | L37 | Shodex Shodex OHpak SB-803HQ |
| Size exclusion Pack for water soluble paints based on methacrylate | L38 | Shodex Shodex OHpak SB-802 HQ |
| Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin | L39 | Shodex Shodex OHpak SB-802 HQ |
| Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin | L39 | Shodex Shodex RSpak DM-614 |
| Cellulose tris-3,5-dimethylphenylcarbamate on porous silica gel, 5 to 20 μm particle size | L40 | Altmann Reprosil Chiral-OM |
| Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel, 5 μm particle size | L41 | Altmann Reprosil-AGP |
| Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel, 5 μm particle size | L41 | Chiral Chiral-AGP |
| Pentafluorophenyl groups are chemically bound to silica gel, 5 to 10 μm particle size | L43 | Altmann Reprosil Fluosil PFP |
| Pentafluorophenyl groups are chemically bound to silica gel, 5 to 10 μm particle size | L43 | Waters XSelect CSH Fl-Ph 5µ |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Altmann RCX-30 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton PRP-X110 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton RCX-10 |
| High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups, 8μm particle size | L47 | Hamilton RCX-30 |
| Amylose tris-3,5-dimethylphenylcarbamate on porous, spherical silica gel, 5 to 10 μm particle size | L51 | Altmann Reprosil Chiral-AM |
| Ovomucoid (chiral recognition protein). Chemically bound to silica particles, approximately 5μm particle size, 120 angstrom pore size | L57 | Agilent Ultron ES-OVM |
| Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the sodium (Na +) form, 7 to 11 μm particle size | L58 | Altmann Reprogel Na+ |
| Spherical, porous silica gel with a covalent surface modification with alkylamide groups with end capping, 3-5 μm particle size | L60 | Altmann Reprosil ABZ-Amid C18 |
| C30 silane is bound to a completely porous silica gel, 3 to 15 μm | L62 | Altmann Stability C30 |