The amount of sorbent and the size of the SPE cartridge is generally dependent on your sample: A simple approximation of how much sample can be loaded on an SPE cartridge is 5-10% of the sorbent weight.
3ml tubes are a popular size of cartridge and a good starting point for many applications. Smaller tube dimensions (1 ml) contain smaller amounts of sorbent and require less solvent for elution. This can make a more efficient separation which is useful if evaporation is required. Larger tubes can be used when larger volumes of solvent are required, for example where there is more than one extraction step.
Where compounds are difficult to retain on a column, then a larger sorbent amount can also be helpful.
|Sample size||Sorbent mass||Tube volume||Minimum Elution volume|
|2.5 - 10 mg||50 - 100 mg||1 ml||100 - 200 µl|
|25 - 100 mg||500 mg||3 ml||1 - 3 ml|
|25 - 100 mg||0.5 - 1 g||6 ml||2 - 6 ml|
|100 - 200 mg||2 g||12 ml||10 - 20 ml|
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