CX-1 pH Gradient Buffer B, pH 10,2, 1 L

Easy to optimize and automate, these buffers are suitable for HPLC separation of most monoclonal antibody (mAb) biotherapeutics. CX-1 pH gradient buffers are ready for use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a variety of mAb charge variants through a general HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.
< br>pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

Robust and reproducible gradients
Prediction of pH of biomolecules< br>Easy method optimization
Easy automation
Applicable to most mAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and expected retention of the charge variants and to use a narrower pH range to achieve a higher resolution separation.