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HPLC columns for ion exchange chromatography

In ion exchange chromatography, the net charge and the charge distribution on the surface of a protein determine the interaction of the protein with the charged groups on the surface of the packing material. The surface charges of protein and packing material must be opposite, so that an interaction can take place. The modification of different parameters, such as the pH value of the buffer, the buffer composition, the gradient of the gradient, and the salt with which the gradient is built, enable a wide range of possibilities for optimizing a successful separation. At each pH, a protein has a positive or negative net charge due to the charge state of the amino acids. Accordingly, this protein, if positively charged, will bind either to negatively charged materials or otherwise to positively charged materials. The separation takes place essentially according to charge and size of the ions. As materials for the gel matrix, resins such as polystyrene, cellulose, and crosslinked polyacrylamide or polydextran gels are used. It is distinguished by strong and weak anion or cation exchangers. Ion exchange chromatography is normally performed at pH values which leave the protein in its active conformation.

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Ion-Exchanger (IEC)

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  1. Altmann Analytik

    HPLC Column Partisil SAX 10µm 250x4

    Regular Price: $906.95

    Special Price $861.60

    sku: AANPSSX-10025040
  2. Agilent Technologies

    HPLC Column Agilent Bio SA x , 4.6 x 250 mm, 5µm, SS

    Regular Price: $1,863.86

    Special Price $1,770.67

    sku: AG5190-2465
  3. Hamilton

    PRP-X100 10µm 4.1x150mm

    Regular Price: $984.82

    Special Price $935.58

    sku: HT79434
  4. Hamilton

    PRP-X200 10µm 4.1x150mm

    Regular Price: $948.61

    Special Price $901.18

    sku: HT79441
  5. Hamilton

    PRP-X100 10µ 4.6x250mm PEEK

    Regular Price: $1,101.21

    Special Price $1,046.15

    sku: HT79455
  6. Shiseido

    Shiseido CAPCELL PAK SCX UG 80Å 5µm, 150mm x 4.6mm

    Regular Price: $608.32

    Special Price $577.90

    sku: SHI-77003
  7. Grace

    AdsorbosphereXL SCX Column 50x4.6mm 5µ old no. 20520

    Regular Price: $545.71

    Special Price $518.42

    sku: GR5125422
  8. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 100x2

    Regular Price: $535.46

    Special Price $508.69

    sku: AANHAPRR3-7010020
  9. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 100x3

    Regular Price: $563.64

    Special Price $535.46

    sku: AANHAPRR3-7010030
  10. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 100x4

    Regular Price: $563.64

    Special Price $535.46

    sku: AANHAPRR3-7010040
  11. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 100x4,6

    Regular Price: $620.00

    Special Price $589.00

    sku: AANHAPRR3-7010046
  12. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 125x2

    Regular Price: $605.91

    Special Price $575.61

    sku: AANHAPRR3-7012520
  13. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 125x3

    Regular Price: $605.91

    Special Price $575.61

    sku: AANHAPRR3-7012530
  14. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 125x4

    Regular Price: $605.91

    Special Price $575.61

    sku: AANHAPRR3-7012540
  15. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 125x4,6

    Regular Price: $634.10

    Special Price $602.40

    sku: AANHAPRR3-7012546
  16. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 150x2

    Regular Price: $634.10

    Special Price $602.40

    sku: AANHAPRR3-7015020
  17. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 150x3

    Regular Price: $690.46

    Special Price $655.94

    sku: AANHAPRR3-7015030
  18. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 150x4

    Regular Price: $690.46

    Special Price $655.94

    sku: AANHAPRR3-7015040
  19. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 150x4,6

    Regular Price: $789.10

    Special Price $749.65

    sku: AANHAPRR3-7015046
  20. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 250x2

    Regular Price: $775.01

    Special Price $736.26

    sku: AANHAPRR3-7025020
  21. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 250x3

    Regular Price: $831.37

    Special Price $789.80

    sku: AANHAPRR3-7025030
  22. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 250x4

    Regular Price: $831.37

    Special Price $789.80

    sku: AANHAPRR3-7025040
  23. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 250x4,6

    Regular Price: $930.01

    Special Price $883.51

    sku: AANHAPRR3-7025046
  24. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 300x2

    Regular Price: $831.37

    Special Price $789.80

    sku: AANHAPRR3-7030020
  25. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 300x3

    Regular Price: $915.92

    Special Price $870.12

    sku: AANHAPRR3-7030030
  26. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 300x4

    Regular Price: $915.92

    Special Price $870.12

    sku: AANHAPRR3-7030040
  27. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 300x4,6

    Regular Price: $972.28

    Special Price $923.67

    sku: AANHAPRR3-7030046
  28. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 40x2

    Regular Price: $436.82

    Special Price $414.98

    sku: AANHAPRR3-704020
  29. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 50x2

    Regular Price: $450.91

    Special Price $428.36

    sku: AANHAPRR3-705020
  30. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 50x3

    Regular Price: $436.82

    Special Price $414.98

    sku: AANHAPRR3-705030
  31. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 50x4

    Regular Price: $436.82

    Special Price $414.98

    sku: AANHAPRR3-705040
  32. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 50x4,6

    Regular Price: $479.09

    Special Price $455.14

    sku: AANHAPRR3-705046
  33. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 75x3

    Regular Price: $535.46

    Special Price $508.69

    sku: AANHAPRR3-707530
  34. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 75x4

    Regular Price: $535.46

    Special Price $508.69

    sku: AANHAPRR3-707540
  35. Altmann Analytik

    HPLC Column Hamilton PRP-RCX-30 7µm 75x4,6

    Regular Price: $563.64

    Special Price $535.46

    sku: AANHAPRR3-707546
  36. Altmann Analytik

    HPLC Column Partisil SAX 10µm 100x2

    Regular Price: $584.14

    Special Price $554.93

    sku: AANPSSX-10010020
  37. Altmann Analytik

    HPLC Column Partisil SAX 10µm 100x3

    Regular Price: $614.88

    Special Price $584.14

    sku: AANPSSX-10010030
  38. Altmann Analytik

    HPLC Column Partisil SAX 10µm 100x4

    Regular Price: $614.88

    Special Price $584.14

    sku: AANPSSX-10010040
  39. Altmann Analytik

    HPLC Column Partisil SAX 10µm 100x4,6

    Regular Price: $676.37

    Special Price $642.55

    sku: AANPSSX-10010046
  40. Altmann Analytik

    HPLC Column Partisil SAX 10µm 125x2

    Regular Price: $661.00

    Special Price $627.95

    sku: AANPSSX-10012520
  41. Altmann Analytik

    HPLC Column Partisil SAX 10µm 125x3

    Regular Price: $661.00

    Special Price $627.95

    sku: AANPSSX-10012530
  42. Altmann Analytik

    HPLC Column Partisil SAX 10µm 125x4

    Regular Price: $661.00

    Special Price $627.95

    sku: AANPSSX-10012540
  43. Altmann Analytik

    HPLC Column Partisil SAX 10µm 125x4,6

    Regular Price: $691.74

    Special Price $657.15

    sku: AANPSSX-10012546
  44. Altmann Analytik

    HPLC Column Partisil SAX 10µm 150x2

    Regular Price: $691.74

    Special Price $657.15

    sku: AANPSSX-10015020
  45. Altmann Analytik

    HPLC Column Partisil SAX 10µm 150x3

    Regular Price: $753.23

    Special Price $715.57

    sku: AANPSSX-10015030
  46. Altmann Analytik

    HPLC Column Partisil SAX 10µm 150x4

    Regular Price: $753.23

    Special Price $715.57

    sku: AANPSSX-10015040
  47. Altmann Analytik

    HPLC Column Partisil SAX 10µm 150x4,6

    Regular Price: $860.83

    Special Price $817.79

    sku: AANPSSX-10015046
  48. Altmann Analytik

    HPLC Column Partisil SAX 10µm 250x2

    Regular Price: $845.46

    Special Price $803.19

    sku: AANPSSX-10025020
  49. Altmann Analytik

    HPLC Column Partisil SAX 10µm 250x3

    Regular Price: $906.95

    Special Price $861.60

    sku: AANPSSX-10025030
  50. Altmann Analytik

    HPLC Column Partisil SAX 10µm 250x4,6

    Regular Price: $1,014.55

    Special Price $963.82

    sku: AANPSSX-10025046
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