Imtakt HPLC Columns
Scherzo C18-Group
The Scherzo C18 phase is the first multi mode ODS phase on the market. Unlike common ODS columns Scherzo HPLC columns can be used without adding ion pair reagents. Furthermore these columns present anions as well as cations.
The hydrophobic property can be compared to other ODS HPLC columns. This way substance analysis combined with conventional ODS phases, like Unison UK-C18, are very effective for process development.
The Scherzo C18 product group can be divided into three packing specifications, which differ in ion capacity.
Scherzo SS-C18
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Scherzo SM-C18
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Scherzo SW-C18
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- ODS column loaded with a high amount of strong ionic ligands
- most effecitve for improved retention of hybrid ions and low ionic samples
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- ODS columns loaded with weak ionic ligands
- particluarly effective for se-parating alkalic/ acid com-posits at neutral pH condi-tions
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- ODS column loaded with extremly strong ionic ligands
- effective for highly ionic elutions
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Forms of separation of Scherzo C18 columns:
The Scherzo C18 product group (SS-C18, SM-C18, SW-C18) is not only composed of ODS ligands, but also of anionic and cationic ligands.
Separation Type
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Static Phase
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Chemical Effect
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reversed phase |
octadecyl |
increasing composition of organic solutions de-creases retention |
anion exchange |
cation |
increased ion strength (salt or acid concentration) decreases retention for acid samples |
cation exchange |
anion |
increasing salt concentration decreases retention for alkalic solutions. |
normale phase |
cation/anion |
polar samples, that can not be absorbed in 100 % aqueous elutents, can be absorbed with an < 50 % solution caused by electrostatic interactions. |