Measurement Systems and Measuring Instruments
Exact measurment systems and mesauring instruments are essential for optimal and correct results. We offer a variety of different products for determining air pressure, refractive index, temperature, pH value, weight and time. At Analytics-Shop you will find refractometers, thermometers, barometers, scales and pH meters from various different brands.
Column description
Here you will find the brand name of the respective manufacturer for the column, e.g. "XBridge" columns from Waters. It is not necessarily recognizable which type of column the respective name refers to. If you have any questions, please do not hesitate to contact us.
Package specification
The packing specification describes the material of the stationary phase. The name indicates whether a functional group is bound to the carrier material (silica gel, polymer) and, if so, which functional group. Depending on the bound functional group (e.g. C18 chain), this results in the separation mode of the column (reversed phase).
Length
Depending on the type of application or system, different lengths of HPLC columns are used. The length of the column together with the internal diameter defines the column volume. For preparative separations, for example, columns from 250 mm in length with a larger internal diameter are often used; this allows more material to be separated in one run. If you have any questions about suitable HPLC column dimensions for your analysis, please do not hesitate to contact us.
Inner diameter
In addition to the length of the column, the inner diameter (ID) determines the column volume. The larger the internal diameter, the larger the volume and the higher the consumption of solvent. The inner diameter also influences the concentration of the analyte in the detector. For analyses where only a small amount of sample is available, it is advisable to use the smallest possible inner diameter. If an existing method is optimized from a larger to a smaller inner diameter, it must be ensured that the flow is adjusted for a comparable separation so that a constant linear velocity u is obtained
Particle Size
The choice of particle size for the column depends initially on the HPLC system used. Particle sizes <2µm can be used with a UHPLC. In an HPLC system, which can operate at pressures of up to 400 bar, particles of 5 µm are commonly used. The smaller the particle in the column, the higher the resulting back pressure in the HPLC system.
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You can also find further information in the catalogues of the respective brands and manufacturers. In the Brands & Catalogues section, we provide all current catalogues for download.
Can we help you choose the right product for your application? Our product experts will be happy to advise you .
