Graduated Pipettes are pipettes equipped with a volume scale and suitable to be used for different liquid volumes. Due to their capacity of different liquid volumes, they are more flexible, but also more inaccurate than bulb pipettes which only have one or two marks for a determined volume.
German physician Heinrich Schnitger developed the microliter or piston stroke pipette (PSP) in 1957. A microliter pipette works more precisely than conventional glass pipettes and is suitable for the dosage of even the smallest volumes from 0.1μl to 5000μl. Microliter pipettes work according to the principle of displacement: The device contains a moving piston that, when pushed down, displaces the air column below it or pulls it upwards in the upward movement and thereby also the liquid to be pipetted into the inserted pipette tip. This pipette tip is a disposable plastic article and is the only component to get in contact with the liquid. Directly displaced piston stroke pipettes are suitable for pipetting even very viscous solutions and use special pipette tips with a built-in piston, which directly comes into contact with the medium and thus eliminates the air cushion. Among other things, we supply microliter pipettes from the well-known manufacturers Eppendorf, Brand, Vitlab and many others.
Sapphire pipettes from Greiner Bio-One are piston-stroke pipettes with variable volume adjustment, for optimal performance, high user comfort and maximum lifetime in daily use. The pipettes are equipped with an ergonomic finger hook. In combination with a state-of-the-art mechanism, the force required for pipetting is reduced, guaranteeing comfortable and fatigue-free pipetting. The 3-position ejector lever allows relaxed handling for both right- and left-handed users.
The pipettes feature a color-coded control knob, a large volume display, a robust ejection mechanism and universal tip holders. The pipettes are fully autoclavable.
Sapphire pipettes meet the requirements of the ISO 8655 standard, which serves as the basis for determining the accuracy and precision of piston-stroke pipettes. When combined with Sapphire pipette tips, they have one of the lowest measurement deviations compared to other manufacturers and, when used together, provide a fully optimized and harmonized liquid handling solution.
Sapphire tips from Greiner Bio-One are available in eight different sizes ranging in volume from 10 μl to 1250 μl, including an extended 10 μl tip for holding smaller sample volumes.
The new generation of Sapphire pipette tips features an improved tip design with a smooth surface to minimize liquid residues and thus the loss of valuable reagents and samples. The Sapphire low-retention surface minimizes sample material sticking to the inside of the tip. This ensures the highest accuracy, even with detergents and other viscous or complex samples.
The slim tip shape can reduce the risk of contamination during pipetting. The tips are made of highly transparent medical grade polypropylene and provide perfect sample visibility. The volume can be read quickly and easily thanks to the printed graduation. The tip sleeve is flexible and thus provides a perfect hold. The universal fit allows use with all common pipettes, including multichannel pipettes.
Sapphire filter tips are particularly safe: they reliably prevent the transfer of biological material and aerosols without any adverse effect on the accuracy of the pipetted volume. The filters are manufactured without additives, so samples can continue to be used even after accidental contact. Tips are free of detectable DNase, RNase, human DNA and PCR inhibitors, pyrogen-free and non-cytotoxic, and tested for leachables.
Greiner Bio-One Sapphire tips are available in sterile and non-sterile versions and are available as standard, filter, low retention and filter-low retention tips. Depending on the version, Sapphire tips are packaged in racks, bags or as refill units.