Solvents for HPLC

With the increasing sensitivity of HPLC systems, it is becoming more and more important to use high-purity solvents in HPLC as mobile phases. In addition to chemicals from various manufacturers, we carry HPLC solvents at special prices from Merck, Riedel-deHaen and J.T. Baker in various degrees of purity:

Degree of purity HPLC grade Gradient grade LC-MS grade

Solutions

2-Propanol, Acetonitril, Methanol

Acetonitril, Methanol, Water

2-Propanol, Acetonitril, Methanol, Water

Properties

  • suitable for isocratic separations
  • designed for UV or fluorescence detection
  • low fluorescence, slight waste steam pressure
  • low acidity and alkalinity

  • high UV permeability for UV-Vis and fluorescence measurements
  • slight influence on the baselinie of UV detection
  • suitable for highly sensitive measure-ments

  • decreasing of trace minerals, organics and ions
  • very low ambient noise
  • suitable for for measurements with high sensitivity

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Product Image of Ethanol absolute for HPLC, Gradient Grade, 2.5 L
Ethanol absolute for HPLC, Gradient Grade, 2.5 L
Item number: AP221086.1612
Brand: Applichem

In stock

€49.75 €135.90
Product Image of ACETONITRILE LC-MS CHROMASOLV, Glass Bottle, 4 x 2.5 L
ACETONITRILE LC-MS CHROMASOLV, Glass Bottle, 4 x 2.5 L
Item number: RD34967-4X2.5L
Brand: Riedel-de Haen

In stock

€279.00 €1,398.45
Product Image of Water for HPLC, LC-MS Grade, 1L glass bottle, orderable only in packs of 6
Water for HPLC, LC-MS Grade, 1L glass bottle, orderable only in packs of 6
Item number: JB9825.1000GL
Brand: J.T. Baker

In stock

€35.91 €44.89
Product Image of 2-Propanol (Iso-Propanol) by Baker HPLC, 2,5L glass bottle, orderable only in packs of 4
2-Propanol (Iso-Propanol) by Baker HPLC, 2,5L glass bottle, orderable only in packs of 4
Item number: JB9095.2500
Brand: J.T. Baker

In stock

€57.20 €154.93
Product Image of N,N-Dimethylformamide HPLC grade,1 L
N,N-Dimethylformamide HPLC grade,1 L
Item number: AP361785.1611
Brand: Applichem

In stock

€56.65 €113.30
Product Image of Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L
Tetrahydrofuran (UV-IR-HPLC-GPC) PAI,2,5 L
Item number: AP361736.1612
Brand: Applichem

In stock

€269.80
Product Image of 2-Propanol (HPLC) PAI,2,5 L
2-Propanol (HPLC) PAI,2,5 L
Item number: AP361090.1612
Brand: Applichem

In stock

€129.50
Product Image of Ethanol absolute for UV, IR, HPLC, 1 L
Ethanol absolute for UV, IR, HPLC, 1 L
Item number: AP361086.1611
Brand: Applichem

In stock

€58.70
Product Image of Methanol G CHROMASOLV, gradient grade, for HPLC, ≥99.9%, Glass Bottle, 4 x 2.5 L
Methanol G CHROMASOLV, gradient grade, for HPLC, ≥99.9%, Glass Bottle, 4 x 2.5 L
Item number: RD34885-4X2.5L
Brand: Riedel-de Haen

In stock

€101.28 €337.59
Product Image of DICHLOROMETHANE, CHROMASOLV, FOR HPLC, ≥99.8%, with AMYLENE AS STABILIZER, Glass Bottle, 2.5 L
DICHLOROMETHANE, CHROMASOLV, FOR HPLC, ≥99.8%, with AMYLENE AS STABILIZER, Glass Bottle, 2.5 L
Item number: RD34856-2.5L
Brand: Riedel-de Haen

In stock

€40.08 €200.38
Product Image of Water for chromatography LiChrosolv, 1 L
Water for chromatography LiChrosolv, 1 L
Item number: MC1153331000
Brand: Merck

In stock

€26.00 €26.80
Product Image of Ethanol gradient grade for liquid chromatography LiChrosolv, 2,5 L
Ethanol gradient grade for liquid chromatography LiChrosolv, 2,5 L
Item number: MC1117272500
Brand: Merck

In stock

€170.75 €284.58
Product Image of 2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
2-Propanol for analysis EMSURE ACS,ISO,Reag. Ph Eur, 5 L
Item number: MC1096345000
Brand: Merck

In stock

€180.62 €187.00
Product Image of HPLC Flushing Solvent
HPLC Flushing Solvent
Item number: AGG1969-85026
Brand: Agilent Technologies

In stock

€176.00
Product Image of Methanol HPLC > 99.9 %, gradient grade, 2,5 L
Methanol HPLC > 99.9 %, gradient grade, 2,5 L
Item number: BK12490.4700
Brand: Bernd Kraft

In stock

€49.59 €55.10
Product Image of 2-Propanol > 99.7 %, (iso-propyl alcohol), for analysis, 25 L
2-Propanol > 99.7 %, (iso-propyl alcohol), for analysis, 25 L
Item number: BK07029.8500
Brand: Bernd Kraft

In stock

€315.00
Product Image of Acetonitril, Baker HPLC Analyzed, Ultra Gradient HPLC Grade 2,5L, HPLC Grade,glass bottle, orderable only in packs of 4
Acetonitril, Baker HPLC Analyzed, Ultra Gradient HPLC Grade 2,5L, HPLC Grade,glass bottle, orderable only in packs of 4
Item number: JB9017.2500
Brand: J.T. Baker

In stock

€62.25 €328.29
Product Image of Acetonitril, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, Far UV for HPLC, orderable only in packs of 4
Acetonitril, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, Far UV for HPLC, orderable only in packs of 4
Item number: JB9012.2500GL
Brand: J.T. Baker

In stock

€44.75 €222.58
Product Image of Methanol, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, orderable only in packs of 4
Methanol, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, orderable only in packs of 4
Item number: JB8402.2500
Brand: J.T. Baker

In stock

€27.25 €81.61
Product Image of Acetonitrile (Reag. Ph. Eur.) for UHPLC, gradient grade (Supergradient), ACS, 2,5 L
Acetonitrile (Reag. Ph. Eur.) for UHPLC, gradient grade (Supergradient), ACS, 2,5 L
Item number: AP221881.1612
Brand: Applichem

In stock

€32.25 €199.00
Product Image of Methanol für UHPLC, gradient grade (Supergradient), ACS, 2,5L
Methanol für UHPLC, gradient grade (Supergradient), ACS, 2,5L
Item number: AP221091.1612
Brand: Applichem

In stock

€14.75 €68.10
Product Image of Water, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, orderable only in packs of 4
Water, Baker HPLC Analyzed, HPLC Gradient Grade 2,5L, orderable only in packs of 4
Item number: JB4218.2500
Brand: J.T. Baker

In stock

€24.75 €46.51
Product Image of Methanol, LC-MS CHROMASOLV(R), ≥99.9%, Glass Bottle, 4 x 2.5 L
Methanol, LC-MS CHROMASOLV(R), ≥99.9%, Glass Bottle, 4 x 2.5 L
Item number: RD34966-4X2.5L
Brand: Riedel-de Haen

In stock

€149.00 €297.19
Product Image of ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, Glass Bottle, 4 x 2.5 L
ACETONITRILE CHROMASOLV FOR HPLC, GRADIENT GRADE, Glass Bottle, 4 x 2.5 L
Item number: RD34851-4X2.5L
Brand: Riedel-de Haen

In stock

€179.00 €1,297.08
Product Image of Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Water for chromatography LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1153332500
Brand: Merck

In stock

€22.25 €55.40
Product Image of Methanol hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Methanol hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1060352500
Brand: Merck

In stock

€39.75 €113.00
Product Image of Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Methanol gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Item number: MC1060072500
Brand: Merck

In stock

€22.25 €112.00
Product Image of Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Acetonitrile gradient grade for liquid chromatography LiChrosolv Reag. Ph Eur, 2,5 L, orderable only in packs of 4
Item number: MC1000302500
Brand: Merck

In stock

€47.25 €359.00
Product Image of Acetonitrile hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Acetonitrile hypergrade for LC-MS LiChrosolv, 2,5 L, orderable only in packs of 4
Item number: MC1000292500
Brand: Merck

In stock

€67.25 €413.00
Product Image of Methanol, Baker HPLC Analyzed, LC-MS Grade 2,5L, glass bottle, orderable only in packs of 4
Methanol, Baker HPLC Analyzed, LC-MS Grade 2,5L, glass bottle, orderable only in packs of 4
Item number: JB9822.2500GL
Brand: J.T. Baker
€90.05 €112.56
Product Image of Acetonitril LC-MS, 2,5L glass bottle, orderable only in packs of 4
Acetonitril LC-MS, 2,5L glass bottle, orderable only in packs of 4
Item number: JB9821.2500GL
Brand: J.T. Baker
€327.75 €409.69
Product Image of TRIFLUOROACETIC ACID, FOR HPLC, Glass Bottle, 250 ml
TRIFLUOROACETIC ACID, FOR HPLC, Glass Bottle, 250 ml
Item number: FL91707-250ML
Brand: Fluka

In stock

€1,263.99
Product Image of TRIFLUOROACETIC ACID, FOR HPLC in AMPOULE
TRIFLUOROACETIC ACID, FOR HPLC in AMPOULE
Item number: FL91707-10X1ML
Brand: Fluka
€360.61
Product Image of SULFURIC ACID 50%, FOR HPLC, Glass Bottle, 500 ml
SULFURIC ACID 50%, FOR HPLC, Glass Bottle, 500 ml
Item number: FL84733-500ML
Brand: Fluka
€359.85
Product Image of SULFURIC ACID 50%, FOR HPLC, Glass Bottle, 100 ml
SULFURIC ACID 50%, FOR HPLC, Glass Bottle, 100 ml
Item number: FL84733-100ML
Brand: Fluka
€90.87
Product Image of Phosphoric acid, for HPLC, 85-90%, Plastic Bottle, 500 ml
Phosphoric acid, for HPLC, 85-90%, Plastic Bottle, 500 ml
Item number: FL79606-500ML
Brand: Fluka
€76.87 €85.41
Product Image of Phosphoric acid, for HPLC, 85-90%, Plastic Bottle, 100 ml
Phosphoric acid, for HPLC, 85-90%, Plastic Bottle, 100 ml
Item number: FL79606-100ML
Brand: Fluka
€34.92 €38.80
Product Image of Sodium hydroxide solution, for HPLC, 50%, Plastic Bottle, 2.5 L
Sodium hydroxide solution, for HPLC, 50%, Plastic Bottle, 2.5 L
Item number: FL71686-2.5L
Brand: Fluka
€176.83
Product Image of Sodium hydroxide solution, for HPLC, 50%, Plastic Bottle, 1 L
Sodium hydroxide solution, for HPLC, 50%, Plastic Bottle, 1 L
Item number: FL71686-1L
Brand: Fluka

In stock

€81.41
Product Image of Sodium phosphate dibasic dihydrate, for HPLC, ≥98.5%, Plastic Bottle, 250 g
Sodium phosphate dibasic dihydrate, for HPLC, ≥98.5%, Plastic Bottle, 250 g
Item number: FL71633-250G
Brand: Fluka

In stock

€98.99
Product Image of SODIUM ACETATE ANHYDROUS, FOR HPLC, Glass Bottle, 50 g
SODIUM ACETATE ANHYDROUS, FOR HPLC, Glass Bottle, 50 g
Item number: FL71185-50G
Brand: Fluka
€84.05
Product Image of SODIUM ACETATE ANHYDROUS, FOR HPLC, Glass Bottle, 250 g
SODIUM ACETATE ANHYDROUS, FOR HPLC, Glass Bottle, 250 g
Item number: FL71185-250G
Brand: Fluka

In stock

€309.48
Product Image of ACETIC ACID SOLUTION, FOR HPLC, Glass Bottle, 500 ml
ACETIC ACID SOLUTION, FOR HPLC, Glass Bottle, 500 ml
Item number: FL45754-500ML
Brand: Fluka
€300.12
Product Image of ACETIC ACID SOLUTION, FOR HPLC, Glass Bottle, 100 ml
ACETIC ACID SOLUTION, FOR HPLC, Glass Bottle, 100 ml
Item number: FL45754-100ML
Brand: Fluka
€75.15
Product Image of TRIFLUOROACETIC ACID, CHROMASOLV(R), FOR HPLC, ≥99.0%, Glass Bottle, 1 L
TRIFLUOROACETIC ACID, CHROMASOLV(R), FOR HPLC, ≥99.0%, Glass Bottle, 1 L
Item number: FL302031-1L
Brand: Fluka
€1,464.47
Product Image of TRIFLUOROACETIC ACID, CHROMASOLV(R), FOR HPLC, >=99.0%, old no. SA302031-100ML
TRIFLUOROACETIC ACID, CHROMASOLV(R), FOR HPLC, >=99.0%, old no. SA302031-100ML
Item number: FL302031-100ML-M
Brand: Fluka
€204.17 €226.86
Product Image of Triethylammine, for HPLC in AMPOULE
Triethylammine, for HPLC in AMPOULE
Item number: FL17924-10X2ML
Brand: Fluka
€156.96
Product Image of AMMONIUM ACETATE PURISS. P. A., FOR HPLC, > 99.0% (NT), Glass Bottle, 50 g
AMMONIUM ACETATE PURISS. P. A., FOR HPLC, > 99.0% (NT), Glass Bottle, 50 g
Item number: FL17836-50G
Brand: Fluka
€83.79 €93.10
Product Image of AMMONIUM ACETATE PURISS. P. A., FOR HPLC, > 99.0% (NT), Glass Bottle, 250 g
AMMONIUM ACETATE PURISS. P. A., FOR HPLC, > 99.0% (NT), Glass Bottle, 250 g
Item number: FL17836-250G
Brand: Fluka
€276.02 €345.02
Product Image of EDTA DISO-SA DIHYDRATE, FOR HPLC, Glass Bottle, 50 g
EDTA DISO-SA DIHYDRATE, FOR HPLC, Glass Bottle, 50 g
Item number: FL03682-50G
Brand: Fluka
€287.89

Information about the different levels of purity for HPLC 

 

For modern HPLC high purity solutions as mobile phases are increasingly needed. Our consumers can mainly choose between three different classes of purity for their individual analysis, HPLC grade, Gradient grade and LC-MS grade.

But whats the difference between the different qualities referring chromatography and between other high purity solvents? And why is it important to choose a solvent with reference to the performed application?

All qualities have the same basic material and a nominal high purity with low traces of xenobiotics (99.8 - 99.9 %, depending on the product) in common. But especially these minimal residue can cause problems in HPLC depending in the technic of detection, so that in this case "pure" is not equal to "pure".

The following describes the differences of the three levels of solvent purity and the reasons for using every quality for your application. 

HPLC grade

HPLC grade describes a solutions quality, that is mainly desinged for isocratic separations applying UV and fluorescence detection. These solvents are especially characerized by high purity combined with high UV permeability at all relevant wave lengths, low inherent fluorescence and waste steam pressure as well as low acidity and alkalinity.

The solvents undergo special treatment stages to remove potentially interferring substances. Thereby disturbing peaks are minimized and a low ambient noise can be enabled.

After the treatment these solutions are tested specificly for aptitude for fluorescence detection in HPLC. Through this quality controll continous purity of solvents as well as reproducability for your measurements can be guaranteed.

Gradient grade

For chromatographical separation in HPLC besides isocratic also gradient elutions are often applied. At these composition of mobile phase doesn´t change wuring measurement., so e.g. of reversed phase chromatography the organic part increases. Using solvents, that are not specially designed for gradient separation, can cause a distinct increasing of the base line at UV detection and can be sensitive for sensitivity of the method.

To ensure optimal chromatography taking gradient into account Gradient grade is further cleaned up. Besides suitability for UV and fluorescence detection all solutions are specially tested for applicability for gradiental separation, so that a charge consistent quality can be guaranteed for Gradient grade solutions.

The following figure shows a gradient profile to demonstrade the extraordinary results that can be attained with acetonitril Gradient grade. Only a low drift of the base line can be recorded.

Fig. 1: Drift of the base line using Gradient grade as solvent. 

LC-MS grade

With the success of (ultra) high pressure liquid chromatography mass spectroscopy (UHPLC-MS) many new requirements arised to the solvent used because of high detection sensitivity.

This way the focus by using mass spectroscopy lies especially on high ionisation efficience with concurrent low ion background. Therefore the solvents are applicable cleaned up. Thereby removing trace elements, organics and (metall) ions has first priority. These can cause an increased ambient noise and adduct formation with the substances, that should be analysed. The result is a decreased detection sensability.

For quality controll all solvents are tested versus Reserpin (m/z 609.49) as standard. The following figure shows the scan of a positiv and negativ ESI and APCi modus. The whole tested mass area displays a low noise ambient as well as no signals cause by impurities.

<

Fig. 2: Noise ambient of LC-MS measurements with LC-MS grade solvents.