SPME

SPME (Solid Phase Micro Extraction) is a procedure of sampling. Later the samples can be analyzed via GC or HPLC. The sampling by SPME, which no solvent, was developed and patented by the chemist Janusz Pawlizyn. Marketing is carried out by the only licensee SUPELCO, which was part of the Sigma-Aldrich group and is now part of Merck.

For more Information about the SPME and coating materials, please click here.

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Product Image of ND20/ND18 20ml SPME vial, 75,5x22,5mm, clear, special crimp neck, 10 x 100 pc
ND20/ND18 20ml SPME vial, 75,5x22,5mm, clear, special crimp neck, 10 x 100 pc
Item number: AAV31202333
Brand: Altmann Analytik
€329.00
Product Image of Inlet Liner, Ultra Inert, splitless, straight, 0.75 x 95 mm, Shimadzu, for SPME P+T, 5 pc/PAK
Inlet Liner, Ultra Inert, splitless, straight, 0.75 x 95 mm, Shimadzu, for SPME P+T, 5 pc/PAK
Item number: AG8001-0162
Brand: Agilent Technologies

In stock

€99.00
Product Image of SMART SPME Arrow 1.10 mm: DVB/PDMS, phase thickness 120 µm, phase length 20 mm, violet
SMART SPME Arrow 1.10 mm: DVB/PDMS, phase thickness 120 µm, phase length 20 mm, violet
Item number: TF36SA12E1-SM
Brand: Thermo Fisher Scientific

In stock

€531.02
Product Image of Smart SPME Fiber, Carbon WR, 95 µm x 10 mm, Dark blue, 3 pc/PAK
Smart SPME Fiber, Carbon WR, 95 µm x 10 mm, Dark blue, 3 pc/PAK
Item number: TF36SP09C3-SM
Brand: Thermo Fisher Scientific
€784.26
Product Image of Magnetic Screw Thread Cap 18mm with PTFE/Silicone for SPME Headspace Vials, 1000/PAK
Magnetic Screw Thread Cap 18mm with PTFE/Silicone for SPME Headspace Vials, 1000/PAK
Item number: RE23093
Brand: Restek
€906.67
Product Image of Vials, crimp top, for Thin Seal, 20 mL, clear glass vial (flat top), closure type
Vials, crimp top, for Thin Seal, 20 mL, clear glass vial (flat top), closure type
Item number: SUSU860104
Brand: Supelco
€86.10
Product Image of Headspace vial, screw top, rounded bottom (vial only), 10 mL, clear glass, thread 18
Headspace vial, screw top, rounded bottom (vial only), 10 mL, clear glass, thread 18
Item number: SUSU860099
Brand: Supelco
€83.64
Product Image of Custodion SPME Syringe, 19 Gauge Blunt Needle, with DVB/PDMS Fiber
Custodion SPME Syringe, 19 Gauge Blunt Needle, with DVB/PDMS Fiber
Item number: PENTSC19SNB171
Brand: PerkinElmer
€1,410.00
Product Image of SPME Syringe 19Ga BT with DVB/PDMS 3 Rep Fibers
SPME Syringe 19Ga BT with DVB/PDMS 3 Rep Fibers
Item number: PENTSC19SN3B171
Brand: PerkinElmer
€1,830.00
Product Image of SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 24 Gauge, for AS und manual Holder, 3/PAK
SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 24 Gauge, for AS und manual Holder, 3/PAK
Item number: SU57931-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 23 Gauge, for AS und manual Holder, 3/PAK
SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 23 Gauge, for AS und manual Holder, 3/PAK
Item number: SU57923-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 24 Gauge, for manual Holder, 3/PAK
SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 24 Gauge, for manual Holder, 3/PAK
Item number: SU57921-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 23 Gauge, for manual Holder, 3/PAK
SPME Fiber Assembly PDMS/DVB, Nitinol-Core, 65 µm, 23 Gauge, for manual Holder, 3/PAK
Item number: SU57916-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 24 Gauge, for AS und manual Holder, 3/PAK
SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 24 Gauge, for AS und manual Holder, 3/PAK
Item number: SU57908-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 23 Gauge, for AS und manual Holder, 3/PAK
SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 23 Gauge, for AS und manual Holder, 3/PAK
Item number: SU57907-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 24 Gauge, for manual Holder, 3/PAK
SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 24 Gauge, for manual Holder, 3/PAK
Item number: SU57904-U
Brand: Supelco
€775.71
Product Image of SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 23 Gauge, for manual Holder, 3/PAK
SPME Fiber Assembly Carboxen/PDMS, Nitinol-Core, 75 µm, 23 Gauge, for manual Holder, 3/PAK
Item number: SU57901-U
Brand: Supelco
€775.71
Product Image of PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Thermo Trace Ultra
PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Thermo Trace Ultra
Item number: RE27364
Brand: Restek
€1,124.00
Product Image of PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Thermo Trace 1300/1310
PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Thermo Trace 1300/1310
Item number: RE27363
Brand: Restek
€2,492.00
Product Image of PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Agilent 7890
PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Agilent 7890
Item number: RE27362
Brand: Restek
€2,539.00
Product Image of PAL SPME Arrow Conversion Kit with 1.5 mmmiroseal Kit for Split/Splitless Injector Agilent 6890
PAL SPME Arrow Conversion Kit with 1.5 mmmiroseal Kit for Split/Splitless Injector Agilent 6890
Item number: RE27361
Brand: Restek
€1,762.00
Product Image of PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Shimadzu 2010
PAL SPME Arrow Conversion Kit with 1.5 mm Microseal Kit for Split/Splitless Injector Shimadzu 2010
Item number: RE27360
Brand: Restek
€2,098.00
Product Image of PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Thermo Trace Ultra
PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Thermo Trace Ultra
Item number: RE27359
Brand: Restek
€1,124.00
Product Image of PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitess Injector Thermo Trace 1300/1310
PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitess Injector Thermo Trace 1300/1310
Item number: RE27358
Brand: Restek
€2,492.00
Product Image of PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Agilent 7890
PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Agilent 7890
Item number: RE27357
Brand: Restek
€2,638.00
Product Image of PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Agilent 6890
PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Agilent 6890
Item number: RE27356
Brand: Restek
€1,762.00
Product Image of PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Shimadzu 2010
PAL SPME Arrow Conversion Kit with 1.1 mm Microseal Kit for Split/Splitless Injector Shimadzu 2010
Item number: RE27355
Brand: Restek
€2,058.00
Product Image of PDMS SPME Fiber, fiber thickness 100µm, fiber length 10mm, red, 3 pc/PAK
PDMS SPME Fiber, fiber thickness 100µm, fiber length 10mm, red, 3 pc/PAK
Item number: TF36SP10P3
Brand: Thermo Fisher Scientific
€693.64
Product Image of PDMS SPME Fiber, fiber thickness 100µm, fiber length 10mm, red, 1 pc/PAK
PDMS SPME Fiber, fiber thickness 100µm, fiber length 10mm, red, 1 pc/PAK
Item number: TF36SP10P1
Brand: Thermo Fisher Scientific
€277.95
Product Image of Carbon WR SPME Fiber, fiber thickness 95µm, fiber length 10mm, dark blue, 3 pc/PAK
Carbon WR SPME Fiber, fiber thickness 95µm, fiber length 10mm, dark blue, 3 pc/PAK
Item number: TF36SP09C3
Brand: Thermo Fisher Scientific
€693.64
Product Image of Carbon WR SPME Fiber, fiber thickness 95µm, fiber length 10mm, dark blue, 1 pc/PAK
Carbon WR SPME Fiber, fiber thickness 95µm, fiber length 10mm, dark blue, 1 pc/PAK
Item number: TF36SP09C1
Brand: Thermo Fisher Scientific
€277.95
Product Image of SPME fibers, selection of 5 different fibers with fiber length 10mm and various. fiber thickness
SPME fibers, selection of 5 different fibers with fiber length 10mm and various. fiber thickness
Item number: TF36SP08M5
Brand: Thermo Fisher Scientific
€1,259.39
Product Image of Acrylate SPME Fiber, fiber thickness 85µm, fiber length 10mm, gray, 3 pc/PAK
Acrylate SPME Fiber, fiber thickness 85µm, fiber length 10mm, gray, 3 pc/PAK
Item number: TF36SP08A3
Brand: Thermo Fisher Scientific
€693.64
Product Image of Acrylate SPME Fiber, fiber thickness 85µm, fiber length 10mm, gray, 1 pc/PAK
Acrylate SPME Fiber, fiber thickness 85µm, fiber length 10mm, gray, 1 pc/PAK
Item number: TF36SP08A1
Brand: Thermo Fisher Scientific
€285.33
Product Image of DVB / PDMS SPME Fiber, fiber thickness 65µm, fiber length 10mm, violet, 1 pc/PAK
DVB / PDMS SPME Fiber, fiber thickness 65µm, fiber length 10mm, violet, 1 pc/PAK
Item number: TF36SP06E3
Brand: Thermo Fisher Scientific
€707.17
Product Image of DVB / PDMS SPME Fiber, fiber thickness 65µm, fiber length 10mm, violet, 1 pc/PAK
DVB / PDMS SPME Fiber, fiber thickness 65µm, fiber length 10mm, violet, 1 pc/PAK
Item number: TF36SP06E1
Brand: Thermo Fisher Scientific
€277.95
Product Image of DVB / Carbon WR / PDMS SPME Fiber, fiber thickness 50/30µm, fiber length 10mm, dark gray, 3 pc/PAK
DVB / Carbon WR / PDMS SPME Fiber, fiber thickness 50/30µm, fiber length 10mm, dark gray, 3 pc/PAK
Item number: TF36SP05T3
Brand: Thermo Fisher Scientific
€693.64
Product Image of DVB / Carbon WR / PDMS SPME Fiber, fiber thickness 50/30µm, fiber length 10mm, dark gray, 1 pc/PAK
DVB / Carbon WR / PDMS SPME Fiber, fiber thickness 50/30µm, fiber length 10mm, dark gray, 1 pc/PAK
Item number: TF36SP05T1
Brand: Thermo Fisher Scientific
€285.33
Product Image of PDMS SPME Fiber, fiber thickness 30µm, fiber length 10mm, golden, 3 pc/PAK
PDMS SPME Fiber, fiber thickness 30µm, fiber length 10mm, golden, 3 pc/PAK
Item number: TF36SP03P3
Brand: Thermo Fisher Scientific
€693.64
Product Image of SPME fibers, selection of 5 different fibers with fiber length 10mm and various. fiber thickness
SPME fibers, selection of 5 different fibers with fiber length 10mm and various. fiber thickness
Item number: TF36SP03M5
Brand: Thermo Fisher Scientific
€1,296.27
Product Image of PDMS SPME Fiber, fiber thickness 7µm, fiber length 10mm, green, 3 pc/PAK
PDMS SPME Fiber, fiber thickness 7µm, fiber length 10mm, green, 3 pc/PAK
Item number: TF36SP01P3
Brand: Thermo Fisher Scientific
€693.64
Product Image of PDMS SPME Fiber, fiber thickness 7µm, fiber length 10mm, green, 1 pc/PAK
PDMS SPME Fiber, fiber thickness 7µm, fiber length 10mm, green, 1 pc/PAK
Item number: TF36SP01P1
Brand: Thermo Fisher Scientific
€285.33
Product Image of PDMS SPME Fiber, fiber thickness 30µm, fiber length 10mm, golden, 1 pc/PAK
PDMS SPME Fiber, fiber thickness 30µm, fiber length 10mm, golden, 1 pc/PAK
Item number: TF36SP03P1
Brand: Thermo Fisher Scientific
€277.95
Product Image of Screw Cap Vial, Amber For SPME, 10ml, 100/PAK
Screw Cap Vial, Amber For SPME, 10ml, 100/PAK
Item number: SUSU860100
Brand: Supelco
€74.39
Product Image of Screw Cap Vial, Amber For SPME, 20ml, 100/PAK
Screw Cap Vial, Amber For SPME, 20ml, 100/PAK
Item number: SUSU860098
Brand: Supelco
€134.71
Product Image of Screw Cap Vial, Clear For SPME, 20ml, 100/PAK
Screw Cap Vial, Clear For SPME, 20ml, 100/PAK
Item number: SUSU860097
Brand: Supelco
€94.89
Product Image of 0.60µm PEG 23Ga SPME FFA, 3/PAK
0.60µm PEG 23Ga SPME FFA, 3/PAK
Item number: SUFFA57354-U
Brand: Supelco
€822.77
Product Image of 0.85µm CAR/PDMS 24Ga SPME FFA, 3/PAK
0.85µm CAR/PDMS 24Ga SPME FFA, 3/PAK
Item number: SUFFA57335-U
Brand: Supelco
€738.26
Product Image of 0.65µm PDMS-DVB 24Ga SPME FFA, 3/PAK
0.65µm PDMS-DVB 24Ga SPME FFA, 3/PAK
Item number: SUFFA57327-U
Brand: Supelco
€738.26
Product Image of 0.30µm PDMS 24Ga SPME FFA, 3/PAK
0.30µm PDMS 24Ga SPME FFA, 3/PAK
Item number: SUFFA57309
Brand: Supelco
€738.26

Samples which are to be analyzed by gas chromatography are usually filled in so-called autosampler vials, which are sealed with a crimp lid. For sampling, an SPME needle is pierced through the septum into the sample vial. An SPME needle looks similar to a normal cannula because it is also made of stainless steel. However, this does not serve to remove or inject fluids, but helps in penetrating the septum. There is a fused silica (fused silica) or glass fiber (StableFlex) in the needle, coated on the end. This coated fiber absorbs analyte molecules from the sample. The sample can either be taken directly from the liquid phase or from the gas phase above the liquid, the so-called head-space. If the sample is to be taken out of the gas phase, a volatility of the analyte has to be given, so that enrichment from the gas phase is possible. More information about Headspace analysis can be found here.

Desorption

Following the extraction of the analyte from the liquid sample, is the desorption in order to release the analytes from the coated fiber of the SPME needle. Different methods are suitable depending on the choice of the desired analytical method. If sample is getting analyzed by of gas chromatography, the desorption can be carried out without further intermediate steps within the sample inlet of the gas chromatograph. In this case, the so called thermal desorption is used, using the high temperature within the chromatograph to dissolve the absorbed analyte molecules from the fiber and to convert them into the gas phase. The steps of thermal desorption of the analytes and transfer into the gas phase for the gas-chromatographic separation are connected and thus analyte loss is avoided or at least minimized. The temperatures of the Inlet depend on the respective temperature during the chromatographic separation, but usually are between 100 and 300 ° C.

The absorbed analyte molecules can also be dissolved from the fiber with the aid of a solvent. This method is used if later analysis is to be carried out by HPLC (high pressure liquid chromatography).

Coating materials

Through the use of different coating materials, substance groups can be selectively absorbed. The commercially available adsorbing materials which differ greatly in their polarity include: carbowax, polydivinylbenzene, polyacrylate, carboxes and PDMS (polydimethylsiloxane). Depending on the analyte, combinations of different layer thicknesses and adsorbing materials are used.

The following table lists the fibers recommended by Merck for various types of analytes (MW = molar mass):

Analyt Type Recommended Fiber
Gases and low molecular weight compounds (MW 30-225) 75 µm/85 µm Carboxen/polydimethylsiloxane
Volatiles (MW 60-275) 100 µm polydimethylsiloxane
Volatiles, amines and nitro-aromatic compounds (MW 50-300) 65 µm polydimethylsiloxane/divinylbenzene
Polar semi-volatiles (MW 80-300) 85 µm polyacrylate
Non-polar high molecular weight compounds (MW 125-600) 7 µm polydimethylsiloxane
Non-polar semi-volatiles (MW 80-500) 30 µm polydimethylsiloxane
Alcohols and polar compounds (MW 40-275) 60 µm Carbowax (PEG)
Flavor compounds: volatiles and semi-volatiles, C3-C20 (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber
Trace compound analysis (MW 40-275) 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a 2 cm StableFlex fiber
Amines and polar compounds (HPLC use only) 60 µm polydimethylsiloxane/divinylbenzene

Advantages of SPME:

  • Solvent-free micro-extraction technology for almost all samples and matrices
  • easy to automate
  • compatible with GC
  • reusable
  • inexpensive
  • fast
  • does not destroy the sample

 

For further information on SPME we recommend the How to get started guide by Merck!

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