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Find and compare HPLC Columns with our HPLC Column Configurator

With the help of our Liquid Chromatography Column Configurator, you can easily find the HPLC column you are looking for. Choose from over 130,000 HPLC columns from more than 50 well-knowned manufacturers and brands, such as Agilent Technologies, Grace, Merck Millipore, Thermo Scientific, Waters etc. In addition, we offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative.

There are many different manufacturers of HPLC columns. If you are looking for a particular HPLC column for your laboratory analysis, we can provide you with an independent offer of comparable columns from different manufacturers. If you are not satisfied with the performance of a column, our sales team will be able to offer you alternatives to suit your requirements.

By using the drop-down menus on the left, you can easily select the column description, packing material, particle size and other specifications as well as the desired manufacturer. If you are unable to find a suitable HPLC column or have any questions, please feel free to contact us.

For use in regulated environments, our HPLC columns are labeled with the universal USP code. Further information on official methods and HPLC column comparisons can be found on the United States Pharmacopeia (USP) website. Furthermore, we have a list of USP column suggestions which can be downloaded here.

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HPLC Column Configurator

 
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  1. Altmann Analytik

    Column Storage Cabinet for 30 columns

    Regular Price: €390.00

    Special Price €350.00

    SKU: AAColStore30
    In Stock
  2. Agilent Technologies

    HPLC Column ZORBAX SB-C18, 80Å, 1,8 µm, 3 x 50 mm, 600 bar, SPL

    €628.00
    SKU: AG884950-576
  3. Altmann Analytik

    HPLC Column Nucleosil 100 C18, 5.0 µm, 4 x 125 mm

    Regular Price: €291.50

    Special Price €279.31

    SKU: AANNS1C9-5012540
    In Stock
  4. Altmann Analytik

    HPLC Column Lichrospher 60 RP-Select-B, 5.0 µm, 4 x 125 mm

    Regular Price: €344.50

    Special Price €316.94

    SKU: AANLI6RS-5012540
    In Stock
  5. Shodex

    HPLC Column Asahipak ODP-50 4E, 250 Å, 5 µm, 4.6 x 250 mm

    Regular Price: €875.56

    Special Price €700.45

    SKU: SDF7620003
    In Stock
  6. YMC

    UHPLC Column YMC-TriArt C18, 1,9µm, 75 x 3.0 mm

    €772.00
    SKU: YMTA12SP9-L503PT
    In Stock
  7. YMC

    HPLC Column YMC-Pack ODS-AQ, 12 nm, 5µm, 250 x 4.6 mm

    €666.00
    SKU: YMAQ12S05-2546WT
    In Stock
  8. Waters

    HPLC Column Symmetry C8 100Å 5µm 4.6x250mm

    €756.00
    SKU: WTWAT054270
    In Stock
  9. Waters

    HPLC Column Symmetry C18, 100Å 5µm 3.9x150mm

    €678.00
    SKU: WTWAT046980
    In Stock
  10. Waters

    HPLC Column XBridge C18, 130Å, 3.5µm 4.6x150mm

    Regular Price: €801.00

    Special Price €704.88

    SKU: WT186003034
    In Stock
  11. Thermo Fisher Scientific

    HPLC Column BDS-HYPERSIL-C18 250x4.6mm 5µm

    €579.13
    SKU: TF28105-254630
    In Stock
  12. Thermo Fisher Scientific

    ProPac WCX-10 Analytical Column(4 x 250 mm)

    Regular Price: €1,096.00

    Special Price €1,008.32

    SKU: TF054993
    In Stock
  13. Merck

    HPLC Column Chromolith Performance RP-18 endcapped 100x4.6mm, monolithic ready-to-use HPLC Column

    Regular Price: €884.00

    Special Price €839.80

    SKU: MC1021290001
  14. Macherey-Nagel

    HPLC Column EC 125/4.6 NUCLEOSIL 100-5 C18

    €316.00
    SKU: MN720002.46
    In Stock
  15. Chiral

    HPLC Column CHIRALPAK® AD-H, 250 x 4,6 mm, 5 µm

    Regular Price: €1,760.00

    Special Price €1,707.20

    SKU: DAIC19325
    In Stock
  16. Agilent Technologies

    HPLC Column ZORBAX RR, SB-C18, 80Å, 3,5 µm, 4,6 x 100 mm

    Regular Price: €586.00

    Special Price €527.40

    SKU: AG861953-902
    In Stock
  17. GL Sciences

    HPLC Column Inertsil ODS-3 100Å, 3µm, 4.6 x 150mm

    €560.00
    SKU: GL5020-01771
    In Stock
  18. GL Sciences

    HPLC Column Inertsil ODS-3 100Å, 5µm, 4.6 x 250mm

    €570.00
    SKU: GL5020-01732
    In Stock
  19. Grace

    HPLC Column Alltima C18 250x4.6mm 5µm old no: 88056

    Regular Price: €808.00

    Special Price €727.20

    SKU: GR5129678
    In Stock
  20. Waters

    HPLC Column Atlantis dc18 3µm 4.6 x 150mm

    €756.00
    SKU: WT186001342
    In Stock
  21. Supelco

    HPLC Column Ascentis Express C18, 2.7 µm, 150 x 4.6 mm

    Regular Price: €724.00

    Special Price €687.80

    SKU: SU53829-U
  22. Supelco

    HPLC Column Discovery® C18, 5 µm, L x ID, 150 x 4.6 mm

    Regular Price: €825.71

    Special Price €784.42

    SKU: SU504955
  23. Osaka Soda (former Shiseido)

    HPLC Column Osaka Soda (Shiseido) CAPCELL PAK C18 SG 120Å 5µm, 250mm x 4.6mm

    Regular Price: €695.36

    Special Price €660.59

    SKU: SHI-12513
  24. Macherey-Nagel

    HPLC Column, EC 250/4.6 NUCLEOSIL 100-10 CN

    €386.00
    SKU: MN720024.46
    In Stock
  25. Macherey-Nagel

    HPLC Column EC 250/4 NUCLEOSIL 100-5 C8

    €365.00
    SKU: MN720013.40
    In Stock
  26. Macherey-Nagel

    HPLC Column EC 125/4 NUCLEOSIL 100-5 C18

    €300.00
    SKU: MN720002.40
    In Stock
  27. Chiral

    HPLC Column CHIRALPAK AGP, 100 x 4 mm, 5 µm

    Regular Price: €1,251.00

    Special Price €1,213.47

    SKU: DAIC30713
    In Stock
  28. Agilent Technologies

    HPLC Column InfinityLab PFC, 4.6 x 30 mm

    Regular Price: €663.00

    Special Price €643.11

    SKU: AG5062-8100
  29. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 5µm C18 AB, 3 x 50 mm

    €351.00
    SKU: MN720957.30
    In Stock
  30. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 3µm C18, 2 x 150 mm

    €427.00
    SKU: MN720949.20
    In Stock
  31. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 10µm C18, 4 x 200 mm

    €365.00
    SKU: MN720704.40
    In Stock
  32. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 3µm C18 HD, 3 x 125 mm

    €645.00
    SKU: MN720191.30
    In Stock
  33. Macherey-Nagel

    HPLC Column Nucleosil EC 120Å 5µm C18, 4 x 30 mm

    €216.00
    SKU: MN720162.40
    In Stock
  34. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 5µm NH2-RP, 3 x 250 mm

    €365.00
    SKU: MN720095.30RP
    In Stock
  35. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 3µm C8, 3 x 125 mm

    €435.00
    SKU: MN720071.30
    In Stock
  36. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 3µm C18, 3 x 125 mm

    €435.00
    SKU: MN720040.30
    In Stock
  37. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 5µm C18, 4.6 x 200 mm

    €386.00
    SKU: MN720006.46
    In Stock
  38. Macherey-Nagel

    HPLC Column Nucleosil EC 100Å 5µm C18, 4 x 200 mm

    €365.00
    SKU: MN720006.40
    In Stock
  39. Altmann Analytik

    HPLC Guard Column Reprosil Pur Basic C18, 100 Å, 3.0 µm, 4.6 x 5 mm, 5pc/PAK

    €270.00
    SKU: AAVRSPUB9-30546-5
    In Stock
  40. Altmann Analytik

    HPLC Column Eurospher C18, 100Å, 5.0 µm, 4 x 150 mm

    €286.00
    SKU: AANEUC9-5015040
  41. Altmann Analytik

    HPLC Column Reprosil 100 C18, 4.0 µm, 4 x 250 mm

    €385.00
    SKU: AANRS1C9-4025040
  42. Grom

    HPLC Column GromSil 120 ODS-5 ST 5µm, 125x4mm

    €350.00
    SKU: GMGSOD50512s1204
    In Stock
  43. Grom

    HPLC Column GromSil 80 Si NP-1 5µm, 250x4.6mm

    €395.00
    SKU: GMGSNP10508s2546
    In Stock
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HPLC Columns for every application

Find the right column from over 130,000 HPLC columns from more than 50 different manufacturers such as Agilent, Macherey-Nagel or Merck Millipore. We also offer our own brand of Altmann Analytik HPLC columns as a high-quality and cost-effective alternative. Compare prices and the application possibilities of the various manufacturers. If you are unable to find a suitable HPLC column, please feel free to contact us.

NP columns for Normal Phase Chromatography

Prior to the development of the Reversed Phase (RP), Normal Phase Chromatography was the most common separation mode. We offer Normal Phase (NP) HPLC columns by all well-known manufacturers here.

In Normal Phase Chromatography, the mobile phase is non-polar and the stationary phase is polar (common: silica gel). This technique relies on the interaction of analytes with polar functional groups on the surface of the stationary phase. This interaction is strongest when using non-polar solvents as the mobile phase. The least polar compounds elute first and the most polar compounds elute last.

Normal Phase Chromatography is a very effective separation method because a wide range of solvents can be used to fine-tune the selectivity of a separation. However, it has become less popular with many chromatographers because of the complexity involved. Under certain conditions, long equilibration times or reproducibility problems may occur. This is due mainly to the sensitivity of the technique to the presence of low concentrations of polar contaminants in the mobile phase. Controlling these problems effectively produces better chromatograms than reverse phase methods as the commonly used solvents have a lower viscosity.

HPLC columns for Reversed Phase (RP) Chromatography

In Reversed Phase Chromatography, the polarity of the phases are "reversed". The mobile phase is polar and the stationary phase is non-polar. As it is one of the most popular separation modes in chromatography, all related products of all well-known manufacturers are available here.

The non-polar side chains in Reversed Phase Chromatography columns are bound either to a polymer or to a structure made of silica gel, which leads to them being hydrophobic. The longer the chain, the more non-polar the phases are. Polar analytes are eluted from the column first, followed by the non-polar analytes. Reversed phase is more widely practised than normal phased as they can be used universally for polar and non-polar analytes. In addition, this method is very sensitive and flexible as small changes in the composition of the mobile phase (e.g. salts, pH, organic solvents) or the temperature can completely change the separation properties of the system. RP-HPLC is used especially in numerous applications of UV spectroscopy (LC-UV).

Chiral columns for Chiral/Enantiomeric chromatography

Unlike normal HPLC columns, chiral or enantiomeric HPLC can also be used to separate and determine chiral compounds. This requires special chiral stationary phases that have fixed chiral functionalities. At Analytics-Shop, we have over 3,300 different chiral columns from different manufacturers, including the high-quality columns from Chiral Technologies as well as lower-priced alternatives of the same quality from YMC and our own Altmann Analytik brand.

Special columns such as chiral or enantiomeric HPLC columns are required to separate chiral compounds. With suitable interaction, these enable the separation of the enantiomers which have almost identical physical and chemical properties. Enantiomers are molecules with a chiral center. Diastereomers are molecules with two or more chiral centers, differ in their chemical properties and can be separated using chromatography. The separation of enantiomers is based on the formation of diastereomers and their interaction with other chiral molecules like chiral stationary phases (e.g. silica gels) in chromatographic separations. A conventional solvent is used as the eluent.

UHPLC and UPLC® columns

With Ultra High Performance Liquid Chromatography (UHPLC), dramatic increases in resolution, speed and sensitivity of HPLC can be achieved by using short and thin columns. The particles of the filling material have diameters of less than 2 µm, which results in an improved separation performance compared to standard HPLC. The increased total surface area of the filling material gives the analyte a higher adsorption ability. Analysis times are reduced due to the shorter columns and less solvent is required.

Columns for Hydrophilic Interaction Chromatography (HILIC)

Hydrophilic interaction chromatography (HILIC) is a popular alternative to normal phase and reverse phase chromatography. Similar to NP, buffer systems are used as stationary phases in HILIC mode. These are aqueous buffer systems with organic solvents, e.g. B. acetonitrile. In HILIC mode, water is the strongest eluant. HILIC columns are suitable for the separation of polar compounds such as carbohydrates, polar metabolites and hydrophilic compounds regardless of charge and molecular size. An extensive range of HILIC columns from established quality manufacturer YMC is available in our shop.

Columns for Supercritical Fluid Chromatography (SFC)

Significant advantages of Supercritical Liquid Chromatography (SFC) columns compared to HPLC columns:

  • Inexpensive: reduced solvent comsumption
  • Environmentally friendly
  • Time-saving: quick separation with high resolution
  • Flexible: chiral and achiral phases

Supercritical fluids show low viscosities and higher diffusivities when used as a mobile phase, resulting in narrower peaks due to rapid diffusion and faster elution and less pressure drop through the column. Supercritical fluids combine the benefits of liquids and gases, hence enabling the SFC technique to combine the best aspects of HPLC and gas chromatography (GC). In most cases, a supercritical fluid such as carbon dioxide is used as the mobile phase. The low viscosity of supercritical carbon dioxide enables analytical separations that are 3-5 times faster than those for normal phase HPLC. The speed of SFC separations, the preservation of organic solvents and more concentrated product fractions make SFC a preferred chromatographic technique for separating and purifying chemical mixtures.

SFC is an environmentally friendly separation technique that makes use of CO2-based mobile phases. The use of high-performance preparative columns (internal diameter of 10 - 50 mm) with a large number of particle sizes from 3 - 20 μm leads to the quick separation and recovery of cleaned components.

Nano HPLC columns

Nano HPLC uses columns with very small inner diameters. Columns with internal diameters of 75 µm, 100 µm or 150 µm are commonly used. Due to the reduction in the internal diameter, injection and flow rates must be reduced which is particularly advantageous when only small or diluted sample quantities are available. The smaller nano HPLC columns lead to an increased sensitivity with lower solvent consumption. Nano columns are able to maintain a high concentration of the injected sample and to direct approx. 40-50 % of the sample to the detector. Nano HPLC has a high separation efficiency compared to the traditional HPLC technique.

Columns for Gel Permeation Chromatography (GPC) & Size Exclusion Chromatography (SEC)

Gel permeation chromatography (GPC) is particularly suitable for the separation of non-polar molecules and can be used for a wide range of solvents, from non-polar organics to aqueous applications. GPC/SEC columns are packed with very small porous beads. In GPC/SEC, the molecules are separated according to size (size exclusion chromatography, SEC). The smaller molecules can enter the pores more easily and therefore spend more time in these pores, eluting last. Conversely, larger molecules spend little if any time in the pores and are eluted quickly. The GPC columns are filled with a microporous packing material and gel, hence the name gel permeation.

Ion Exchange Chromatography (IEC)

Ion exchange chromatography (IEC) separates molecules based on the respective charged groups on the surface of a protein. Molecules undergo electrostatic interactions wirh opposite charges on the stationary phase matrix.

The pH value of the buffer, the buffer concentration, packing of the columns and the salt gradient, in which the salt ions compete with the desired proteins to bind to charged groups on the surface of the medium, are important aspects affecting the results of IEC. At any pH, a protein has a net positive or negative charge due to the state of charge of the amino acids. If this protein is positively charged, it will bind to negatively charged materials and vice versa. The separation is based essentially on the charge and size of the ions.

Resins such as polystyrene, cellulose and crosslinked polyacrylamide or polydextran gels are used as mediums for ion exchange. The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged and anion-exchange chromatography is when the stationary phase is positively charged. Ion exchange chromatography is usually performed at pH values in which the protein has a net charge opposite to that of the stationary phase matrix.

Columns with polar bound phases

Polar-bound phases are based on silica gels which have chains attached onto the silica structure with functional groups. Chromatography makes use of the difference in polarity to separate a mixture. Separation takes place through different mechanisms and often as a result of the combination of several processes like adsorption, distribution, ion exchange, molecular size exclusion etc.

Permitted changes according to USP (United States Pharmacopeia)

The U.S. Pharmacopeia Convention is a scientific non-profit company that sets the standards for ingredients, concentration, quality and purity of medicines, food ingredients and food supplements that are manufactured, distributed and consumed all over the world. According to USP regulations, the following deviations may occur:

USP Chapter

  1. Column Length± 70%
  2. Column Inner Diameter: can be changed if the flow rate is kept constant.
  3. Particle Size: - 50%
  4. Flow Rate± 50%
  5. Ratio of components in the mobile phase± 30% or ± 10%
  6. pH value of the mobile phase± 0,2
  7. Salt concentration in the buffer± 10%
  8. Column Temperature± 10°C
  9. Wave Length of the UV-Vis detector± 3 nm
  10. Injection Volume: can be reduced until precision and detection limits are reached.

Permitted changes according to EP (European Pharmacopeia)

European Pharmacopeia Ph. Eur., Chapter 2.2.46

The European Pharmacopeia is a published collection of monographs describing the individual and general quality standards of ingredients, dosages and analytical methods of medicine. The aim is to set common quality standards across Europe to control the quality of medicines and other chemical products. According to the EP regulation, the following deviations can occur:

Isocratic elution

  1. Column Length: ± 70%
  2. Column Inner Diameter: ± 25%
  3. Particle Size: - 50%
  4. Flow Rate: ± 50%
  5. Ratio to components in the mobile phase: ± 30% or ± 2% absolute
  6. pH value of the mobile phase: ±0,2
  7. Salt concentration in the buffer: ± 10%
  8. Column Temperature: ± 10°C
  9. Wave Lenght of the detector: ± 3 nm
  10. Injection Volume: can be reduced until precision and detection limits are reached.

Gradient elution

  1. Column Length: ± 70%
  2. Column Inner Diameter: ± 25%
  3. Particle Size: no changes permitted
  4. Flow Rate: changes acceptable if column size is changed
  5. Ratio to components in the mobile phase: small changes in the composition of the mobile phase and the (density) grade are acceptable if the system configuration still meets the requirements.
  6. Dwell Volume: gradient instants can be used to compensate for differences in dwell volume between different systems.
  7. pH value of the mobile phase: no changes permitted
  8. Salt concentration in the buffer: no changes permitted
  9. Column Temperature: ± 5°C
  10. Wave Lenght of the detector: no deviations permitted
  11. Injection Volume: can be reduced until precision and detection limits are reached.
Phase Name USP Number Column Recommendations
Octadecylsilane chemically bound to porous silica gel L1 Potential Columns
Porous silica gel L3 Potential Columns
Octylsilane was chemically bound to completely porous silica gel L7 Potential Columns
A substantially monomolecular layer of aminopropylsilane, chemically bound to completely porous silica gel L8 Potential Columns
Broken or spherical, completely porous silica gel, with chemically bound, strongly acidic cation exchanger L9 Potential Columns
Nitrile groups chemically bound to porous silica gel L10 Potential Columns
Phenyl groups chemically bound to porous silica gel L11 Potential Columns
Trimethyl groups chemically bound to porous silica gel L13 Potential Columns
Silica gel with chemically bound, strongly basic, quaternary ammonium ion exchanger L14 Potential Columns
Methylsilane groups chemically bound to completely porous silica gel L15 Potential Columns
Dimethylsilane chemically bound to porous silica gel L16 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the hydrogen (H +) form L17 Potential Columns
Amino and cyano groups chemically bound to porous silica gel, 3 to 10 μm particle size L18 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the calcium (Ca2 +) form L19 Potential Columns
Dihydroxypropane groups chemically bound to porous silica gel L20 Potential Columns
Stable, spherical styrene-divinylbenzene copolymer L21 Potential Columns
A cation exchange resin of porous polystyrene having sulfonic acid groups L22 Potential Columns
Ion exchange resin of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups L23 Potential Columns
Pack with the ability to separate compounds (in a molecular weight range of 100 to 5000 daltons (determined with polyethylene oxide) applied to neutral, anionic and cationic water-soluble polymers. Polymethacrylic resin cross-linked with polyhydroxilated ether (surface containing residual carboxyl group content) was found to be appropriate L25 Potential Columns
Methylsilane is chemically bound to completely porous silica gel L26 Potential Columns
Chiral ligand exchange material with L-proline copper complex covalently bound to broken silica gel L32 Potential Columns
Strong cation exchange resin from sulfonated cross-linked PS / DVB copolymer in lead (Pb) form L34 Potential Columns
Polymethacrylate gel pack with the ability to separate proteins in a molecular weight range between 2,000 and 40,000 daltons by molecular size. L37 Potential Columns
Size exclusion Pack for water soluble paints based on methacrylate L38 Potential Columns
Hydrophilic Polyhydroxy Methacrylate gel of completely porous, spherical resin L39 Potential Columns
Cellulose tris-3,5-dimethylphenylcarbamate on porous silica gel L40 Potential Columns
Immobilized α 1 -acid glycoprotein (α-AGP) on spherical silica gel L41 Potential Columns
Pentafluorophenyl groups are chemically bound to silica gel L43 Potential Columns
High-capacity anion exchanger, microporous substrate, fully functionalized with triethylamine groups L47 Potential Columns
Amylose tris-3,5-dimethylphenylcarbamate on porous, spherical silica gel L51 Potential Columns
Ovomucoid (chiral recognition protein). Chemically bound to silica particles L57 Potential Columns
Strong cation exchange resin from a sulfonated cross-linked PS / DVB copolymer in the sodium (Na +) form L58 Potential Columns
Spherical, porous silica gel with a covalent surface modification with alkylamide groups with end capping L60 Potential Columns
C30 silane is bound to a completely porous silica gel L62 Potential Columns

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