Oxoid AnaeroGen 2.5 l bags, 10 bags/pak, for usage with Anaerobic container with volume of 2-3L, Durability days: 610

For the generation of an anaerobic atmosphere in the cultivation of anaerobes. With the OXOID AnaeroGen, anaerobic conditions for the cultivation of fastidious anaerobes can be achieved quickly and reliably. The AnaeroGen bag rapidly absorbs the atmospheric oxygen present in the sealed anaerobic pot and simultaneously evolves carbon dioxide. This new product differs from conventional gas generators in that no hydrogen is released during the chemical reaction, so no catalyst is needed. Furthermore, the addition of water is not necessary.

AnaeroGen reduces the atmospheric oxygen content in a closed anaerobic digester to below 1% within 30 minutes. The carbon dioxide content is then between 9–13%, and the pressure usually drops to about -0.2 bar. Each AnaeroGen paper bag is individually wrapped in foil. The reaction starts as soon as the AnaeroGen paper bag is exposed to air. Therefore, it is absolutely necessary to remove the paper bag immediately after opening the outer foil, place it in the anaerobic digester and close the lid. This process should not take longer than one minute. The reaction of ascorbic acid with oxygen is exothermic, and the temperature of the AnaeroGen paper bag should not exceed 60°C.

Application

• Place inoculated plates in the appropriate anaerobic well. Petri dishes that allow gas exchange should be used, e.g. plastic Petri dishes with aeration cams.
• Päckchen with anaerobic indicator öffnen and pull out the strip about 10 mm. Insert the indicator päckchen into the smaller clamp of the plate rack.
• Tear open the foil of an AnaeroGen bag at the marked point and remove the AnaeroGen paper bag.
• Fix the AnaeroGen paper bag immediately in the corresponding holder of the plate rack.
• Close the anaerobic digester as quickly as possible.
• Remove the plates from the anaerobic digester after the corresponding incubation time and read off. If a new incubation is required, a new AnaeroGen bag must be used (see 1–4).
• Used AnaeroGen bags can be disposed of with the solid laboratory waste.

Quality control In the laboratory, the anaerobic system used should be checked at certain intervals to see whether the appropriate
atmospheric conditions are achieved for the respective microorganisms. Recommended control strains are:

• Clostridium novyii ATCC 9690 growth
• Micrococcus luteus ATCC 9341 no growth

Storage and Shelf Life

• Storage: 2–25°C.
• Shelf life: see label.

Additional notes The AnaeroGen- paper bag heats up as soon as it is exposed to air. Opening the foil, inserting and closing the anaerobic cup should not take longer than one minute. If the bag is exposed to air for longer than about one minute, reactivity will decrease and anaerobic conditions may not be achieved. The AnaeroGen paper bags may still be slightly reactive after removal from the anaerobic digester and should be allowed to cool to room temperature before disposal.