The ACQUITY Premier Peptide HSS T3 Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes such as phosphorylated peptides. The unique column design includes a fully integrated and easily replaceable 5mm pre-column that serves to protect and extend the life of the full-length analytical column, but adds no dispersion and has no impact on the resolution of your analytical separations. VanGuard FIT Cartridge replacements contain the same qualified sorbent as the analytical column and can easily be replaced.