HPLC Column YMC-Pack CN, Microbore, 12nm, S-5 µm, 35 x 3.0 mm

YMC-Pack CN (Cyano): Features & Benefits

In reversed-phase mode, cyano columns are the most polar and least retentive of all reversed-phase packings.  Extremely hydrophobic compounds which do not elute in standard C18 and C8 packings can be retained using typical reversed-phase eluents.  In addition, the unique nitrile chemistry provides different selectivity from both phenyl and standard aliphatic (C18, C8, and C4) reversed-phase packings.  Large pore-size 300Å packing materials are also available for the analysis of biomolecules in order to utilize this unique selectivity.

In normal-phase mode, cyano is a bonded phase that can be used as an alternative to silica.  Bonded normal-phase columns have the advantage of faster equilibration and more uniform surface activity compared to bare silica gel.

To extend column lifetime, continued switching back and forth between normal- and reversed-phase solvents should be avoided.

• Useful in both normal- and reversed-phase applications
• Stable, high coverage, monomerically-bonded phase
• Fully endcapped
• Alternative selectivity
• Available in 300Å for biomolecule analysis

Useful in Both Normal-Phase and Reversed-Phase Modes

YMC-Pack CN can be used for both normal-phase and reversed-phase analyses due to the medium polarity of the cyanopropyl phase bonded to the silica base particle.  It can be used in normal-phase with low-polarity mobile phases such as hexane, or in reversed-phase with highly-polar mobile phases such as methanol and water.  YMC-Pack CN can be useful for shortening analysis time in situations where retention is substantial using C18 or C8, or when separation optimization is difficult.