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Thin-layer chromatography is performed on a glass, plastic, or aluminium plate, which is coated with a thin layer of adsorbent material. The sample is applied on one end of the plate, and a suitable solvent is allowed to rise up the plate by capillary forces. Since different substances are moving up the thin-layer chromatography plate at different rates, they can be separated, identified and analyzed.
Advantages in TLC Method of Analysis: