Wort broth (base) is used for the cultivation, isolation, and enumeration or enrichment of yeasts. The addition of certain indicator dyes to the Wort broth base allows differentiation between yeast and bacterial colonies. The accompanying bacterial flora is weakly suppressed by the pH value of 5.0 and largely by a pH of 3.5. Wort broth (base) is suitable for the cultivation, isolation, and enumeration or enrichment of fungi, especially yeasts.