Isolate and detect Listeria monocytogenes in food samples using Thermo Scientificâ„¢ Chromogenic Listeria Agar (ISO), a specially formulated medium according to ISO 11290-1 and 11290-2. Expression of the enzyme phospholipase C, produced by L. monocytogenes and Listeria ivanovii, creates a distinct opaque white halo around the L. monocolony as a diagnostic criterion for differentiating non-pathogenic Listeria spp. from pathogenic Listeria spp.